Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation
We describe a one-step microfludics-based immunomagnetic isolation method to isolate CTCs directly from the whole blood of lung adenocarcinoma patients. This method avoids harsh sample preparation and enrichment steps, and therefore preserves the viability of CTCs during the in vitro isolation. Impo...
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| Veröffentlicht in: | Analyst (London) 2016-01, Vol.141 (12), p.3621-3625 |
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| container_issue | 12 |
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| container_title | Analyst (London) |
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| creator | Wang, Zhihua Wu, Wenjun Wang, Zhuo Tang, Ying Deng, Yuliang Xu, Ling Tian, Jianhui Shi, Qihui |
| description | We describe a one-step microfludics-based immunomagnetic isolation method to isolate CTCs directly from the whole blood of lung adenocarcinoma patients. This method avoids harsh sample preparation and enrichment steps, and therefore preserves the viability of CTCs during the in vitro isolation. Importantly, isolated, magnetic bead-bearing CTCs are concentrated in a small volume of culture medium with a high CTC density. High cell viability and culturing density promote the ex vivo expansion of limited numbers of CTCs. Expanded CTCs are characterized at the genetic, protein and metabolic levels. |
| doi_str_mv | 10.1039/c5an02554k |
| format | Article |
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This method avoids harsh sample preparation and enrichment steps, and therefore preserves the viability of CTCs during the in vitro isolation. Importantly, isolated, magnetic bead-bearing CTCs are concentrated in a small volume of culture medium with a high CTC density. High cell viability and culturing density promote the ex vivo expansion of limited numbers of CTCs. 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Expanded CTCs are characterized at the genetic, protein and metabolic levels.</description><subject>Blood</subject><subject>Cell Count</subject><subject>Cell Culture Techniques</subject><subject>Culture</subject><subject>Density</subject><subject>Genetics</subject><subject>Humans</subject><subject>Immunomagnetic Separation</subject><subject>Lung Neoplasms - diagnosis</subject><subject>Lungs</subject><subject>Microfluidic Analytical Techniques</subject><subject>Neoplastic Cells, Circulating</subject><subject>Preserves</subject><subject>Proteins</subject><subject>Viability</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1OwzAQhC0EoqVw4QGQjwgpYMex4xxRVX5EBRc4R45jV4bYDnFclbfHpYUrl12t5tNIMwvAOUbXGJHqRlLhUE5p8XEAppiwIqM054dgihAiWc5oMQEnIbynEyOKjsEkZ5yXZZVPQb_YwLVZe6g2vXDBeAe9htIMMnZiNG4Fu5jGGK0foFRdF2AjgmrhFnQqC6PqoTVy8LqLpjUyZDvdWBudt2Ll1GgkNMFv_bw7BUdadEGd7fcMvN0tXucP2fLl_nF-u8wk4WzMSEuaVuSskUQrTpDmTCspWyob0ciiLIuKo0qjJIgCN6XCpBKkyCsmsJK4JDNwufPtB_8ZVRhra8I2gHDKx1BjnhpDnBH0P1pWNNXFK5rQqx2aAocwKF33g7Fi-KoxqrfPqOf09vnnGU8Jvtj7xsaq9g_9bZ98A-Zxhv0</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Wang, Zhihua</creator><creator>Wu, Wenjun</creator><creator>Wang, Zhuo</creator><creator>Tang, Ying</creator><creator>Deng, Yuliang</creator><creator>Xu, Ling</creator><creator>Tian, Jianhui</creator><creator>Shi, Qihui</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20160101</creationdate><title>Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation</title><author>Wang, Zhihua ; Wu, Wenjun ; Wang, Zhuo ; Tang, Ying ; Deng, Yuliang ; Xu, Ling ; Tian, Jianhui ; Shi, Qihui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-3d3bda26bc3fe830f86feccd5cbabc47749809f00f8a41b7e139a34296a1ec173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Blood</topic><topic>Cell Count</topic><topic>Cell Culture Techniques</topic><topic>Culture</topic><topic>Density</topic><topic>Genetics</topic><topic>Humans</topic><topic>Immunomagnetic Separation</topic><topic>Lung Neoplasms - diagnosis</topic><topic>Lungs</topic><topic>Microfluidic Analytical Techniques</topic><topic>Neoplastic Cells, Circulating</topic><topic>Preserves</topic><topic>Proteins</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Zhihua</creatorcontrib><creatorcontrib>Wu, Wenjun</creatorcontrib><creatorcontrib>Wang, Zhuo</creatorcontrib><creatorcontrib>Tang, Ying</creatorcontrib><creatorcontrib>Deng, Yuliang</creatorcontrib><creatorcontrib>Xu, Ling</creatorcontrib><creatorcontrib>Tian, Jianhui</creatorcontrib><creatorcontrib>Shi, Qihui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Zhihua</au><au>Wu, Wenjun</au><au>Wang, Zhuo</au><au>Tang, Ying</au><au>Deng, Yuliang</au><au>Xu, Ling</au><au>Tian, Jianhui</au><au>Shi, Qihui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>141</volume><issue>12</issue><spage>3621</spage><epage>3625</epage><pages>3621-3625</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>We describe a one-step microfludics-based immunomagnetic isolation method to isolate CTCs directly from the whole blood of lung adenocarcinoma patients. 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| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Blood Cell Count Cell Culture Techniques Culture Density Genetics Humans Immunomagnetic Separation Lung Neoplasms - diagnosis Lungs Microfluidic Analytical Techniques Neoplastic Cells, Circulating Preserves Proteins Viability |
| title | Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation |
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