Extractable and non-extractable polyphenols from blueberries modulate LPS-induced expression of iNOS and COX-2 in RAW264.7 macrophages via the NF-κB signalling pathway
BACKGROUND Plant polyphenols are rich in blueberries that have a wide range of properties beneficial to human health. There are two types, according to the solubility of polyphenols, which were defined as extractable polyphenols (EPP) and non‐extractable polyphenols (NEPP), respectively. At present,...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2016-08, Vol.96 (10), p.3393-3400 |
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| creator | Cheng, Anwei Han, Caijing Fang, Xixiu Sun, Jinyue Chen, Xiangyan Wan, Fachun |
| description | BACKGROUND
Plant polyphenols are rich in blueberries that have a wide range of properties beneficial to human health. There are two types, according to the solubility of polyphenols, which were defined as extractable polyphenols (EPP) and non‐extractable polyphenols (NEPP), respectively. At present, in most of reports, ‘total polyphenol’ refers only to EPP excluding NEPP. In this paper, the effects of EPP and NEPP on lipopolysaccharides (LPS) induced production of nitric oxide (NO) and gene expression of inducible NO synthase (iNOS) and cyclooxygenase‐2 (COX‐2) in RAW264.7 cells via nuclear factor‐κB (NF‐κB) signalling pathway were compared.
RESULTS
The results showed that EPP and NEPP from blueberries significantly inhibited the LPS‐induced production of NO and gene expression of iNOS and COX‐2 in cells. The constitutive level of p65 sub‐unit of NF‐κB was obviously detected after the treatments with EPP or NEPP. By contrast, the level of phosphorylated p65 (P‐p65) was strongly inhibited by EPP or NEPP. EPP had a stronger inhibition on the gene expression of iNOS and COX‐2 than that of NEPP.
CONCLUSION
These findings of inhibition of iNOS and COX‐2 mRNA expression through the suppression of NF‐κB suggest that EPP and ENPP from blueberries have significant anti‐inflammatory effect and may be a potential medicine. © 2015 Society of Chemical Industry |
| doi_str_mv | 10.1002/jsfa.7519 |
| format | Article |
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Plant polyphenols are rich in blueberries that have a wide range of properties beneficial to human health. There are two types, according to the solubility of polyphenols, which were defined as extractable polyphenols (EPP) and non‐extractable polyphenols (NEPP), respectively. At present, in most of reports, ‘total polyphenol’ refers only to EPP excluding NEPP. In this paper, the effects of EPP and NEPP on lipopolysaccharides (LPS) induced production of nitric oxide (NO) and gene expression of inducible NO synthase (iNOS) and cyclooxygenase‐2 (COX‐2) in RAW264.7 cells via nuclear factor‐κB (NF‐κB) signalling pathway were compared.
RESULTS
The results showed that EPP and NEPP from blueberries significantly inhibited the LPS‐induced production of NO and gene expression of iNOS and COX‐2 in cells. The constitutive level of p65 sub‐unit of NF‐κB was obviously detected after the treatments with EPP or NEPP. By contrast, the level of phosphorylated p65 (P‐p65) was strongly inhibited by EPP or NEPP. EPP had a stronger inhibition on the gene expression of iNOS and COX‐2 than that of NEPP.
CONCLUSION
These findings of inhibition of iNOS and COX‐2 mRNA expression through the suppression of NF‐κB suggest that EPP and ENPP from blueberries have significant anti‐inflammatory effect and may be a potential medicine. © 2015 Society of Chemical Industry</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.7519</identifier><identifier>PMID: 26538333</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Anti-Inflammatory Agents ; Blueberries ; Blueberry Plants ; Cyclooxygenase 2 - genetics ; cyclooxygenase-2 ; extractable polyphenols ; Foods ; Fruit - chemistry ; Gene expression ; Gene Expression - drug effects ; inducible nitric oxide synthase ; Inhibition ; Lipopolysaccharides - pharmacology ; Macrophages - drug effects ; Macrophages - metabolism ; Mice ; NF-kappa B - antagonists & inhibitors ; NF-kappa B - metabolism ; Nitric Oxide Synthase Type II - genetics ; non-extractable polyphenols ; nuclear factor-κB ; Pathways ; Phosphorylation - drug effects ; Polyphenols ; Polyphenols - isolation & purification ; Polyphenols - pharmacology ; RAW 264.7 Cells ; RNA, Messenger - analysis ; Signal Transduction - drug effects ; Signalling ; Solubility ; Transcription Factor RelA - metabolism ; Vaccinium</subject><ispartof>Journal of the science of food and agriculture, 2016-08, Vol.96 (10), p.3393-3400</ispartof><rights>2015 Society of Chemical Industry</rights><rights>2015 Society of Chemical Industry.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.7519$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.7519$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26538333$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheng, Anwei</creatorcontrib><creatorcontrib>Han, Caijing</creatorcontrib><creatorcontrib>Fang, Xixiu</creatorcontrib><creatorcontrib>Sun, Jinyue</creatorcontrib><creatorcontrib>Chen, Xiangyan</creatorcontrib><creatorcontrib>Wan, Fachun</creatorcontrib><title>Extractable and non-extractable polyphenols from blueberries modulate LPS-induced expression of iNOS and COX-2 in RAW264.7 macrophages via the NF-κB signalling pathway</title><title>Journal of the science of food and agriculture</title><addtitle>J. Sci. Food Agric</addtitle><description>BACKGROUND
Plant polyphenols are rich in blueberries that have a wide range of properties beneficial to human health. There are two types, according to the solubility of polyphenols, which were defined as extractable polyphenols (EPP) and non‐extractable polyphenols (NEPP), respectively. At present, in most of reports, ‘total polyphenol’ refers only to EPP excluding NEPP. In this paper, the effects of EPP and NEPP on lipopolysaccharides (LPS) induced production of nitric oxide (NO) and gene expression of inducible NO synthase (iNOS) and cyclooxygenase‐2 (COX‐2) in RAW264.7 cells via nuclear factor‐κB (NF‐κB) signalling pathway were compared.
RESULTS
The results showed that EPP and NEPP from blueberries significantly inhibited the LPS‐induced production of NO and gene expression of iNOS and COX‐2 in cells. The constitutive level of p65 sub‐unit of NF‐κB was obviously detected after the treatments with EPP or NEPP. By contrast, the level of phosphorylated p65 (P‐p65) was strongly inhibited by EPP or NEPP. EPP had a stronger inhibition on the gene expression of iNOS and COX‐2 than that of NEPP.
CONCLUSION
These findings of inhibition of iNOS and COX‐2 mRNA expression through the suppression of NF‐κB suggest that EPP and ENPP from blueberries have significant anti‐inflammatory effect and may be a potential medicine. © 2015 Society of Chemical Industry</description><subject>Animals</subject><subject>Anti-Inflammatory Agents</subject><subject>Blueberries</subject><subject>Blueberry Plants</subject><subject>Cyclooxygenase 2 - genetics</subject><subject>cyclooxygenase-2</subject><subject>extractable polyphenols</subject><subject>Foods</subject><subject>Fruit - chemistry</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>inducible nitric oxide synthase</subject><subject>Inhibition</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - metabolism</subject><subject>Mice</subject><subject>NF-kappa B - antagonists & inhibitors</subject><subject>NF-kappa B - metabolism</subject><subject>Nitric Oxide Synthase Type II - genetics</subject><subject>non-extractable polyphenols</subject><subject>nuclear factor-κB</subject><subject>Pathways</subject><subject>Phosphorylation - drug effects</subject><subject>Polyphenols</subject><subject>Polyphenols - isolation & purification</subject><subject>Polyphenols - pharmacology</subject><subject>RAW 264.7 Cells</subject><subject>RNA, Messenger - analysis</subject><subject>Signal Transduction - drug effects</subject><subject>Signalling</subject><subject>Solubility</subject><subject>Transcription Factor RelA - metabolism</subject><subject>Vaccinium</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks1y0zAUhT0MDA2FBS_AaMnGqX4sWVqGTBPoZBIgMO1OI8lKoiJLxrJp8kY8Aw_BMxGT0mEHq3vnnu-czT1Z9hLBMYIQX9ymjRqXFIlH2QhBUeYQIvg4Gx01nFNU4LPsWUq3EEIhGHuanWFGCSeEjLLvl_uuVaZT2lugQgVCDLn969ZEf2h2NkSfwKaNNdC-t9q2rbMJ1LHqveosWLxf5y5UvbEVsPumtSm5GEDcALdcrX8HT1c3OQYugI-Ta8yKcQlqZdrY7NT2mPTNKdDtLFjO8p8_3oDktkF578IWNKrb3anD8-zJRvlkX9zP8-zz7PLT9G2-WM3fTSeL3BWYityQEmOuEGeVYQpRVBmjEROaC0qEhiXhHFKNFdKIo2qDmNWGCIxooU0BFTnPXp9ymzZ-7W3qZO2Ssd6rYGOfJOKYFpyTkvwHCjkTBBH6b7QUlDNcsgF9dY_2uraVbFpXq_Yg__zsCFycgDvn7eFBR1AOZZBDGeRQBnm1nk2G5ejITw6XOrt_cKj2i2QlKam8Xs4lmos5_3Al5A35BbiGth0</recordid><startdate>20160801</startdate><enddate>20160801</enddate><creator>Cheng, Anwei</creator><creator>Han, Caijing</creator><creator>Fang, Xixiu</creator><creator>Sun, Jinyue</creator><creator>Chen, Xiangyan</creator><creator>Wan, Fachun</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>20160801</creationdate><title>Extractable and non-extractable polyphenols from blueberries modulate LPS-induced expression of iNOS and COX-2 in RAW264.7 macrophages via the NF-κB signalling pathway</title><author>Cheng, Anwei ; Han, Caijing ; Fang, Xixiu ; Sun, Jinyue ; Chen, Xiangyan ; Wan, Fachun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i4259-c37228a186dc6a151dccb169b89539b0738805b2a1b181df16ebc392154bc40a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents</topic><topic>Blueberries</topic><topic>Blueberry Plants</topic><topic>Cyclooxygenase 2 - genetics</topic><topic>cyclooxygenase-2</topic><topic>extractable polyphenols</topic><topic>Foods</topic><topic>Fruit - chemistry</topic><topic>Gene expression</topic><topic>Gene Expression - drug effects</topic><topic>inducible nitric oxide synthase</topic><topic>Inhibition</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - metabolism</topic><topic>Mice</topic><topic>NF-kappa B - antagonists & inhibitors</topic><topic>NF-kappa B - metabolism</topic><topic>Nitric Oxide Synthase Type II - genetics</topic><topic>non-extractable polyphenols</topic><topic>nuclear factor-κB</topic><topic>Pathways</topic><topic>Phosphorylation - drug effects</topic><topic>Polyphenols</topic><topic>Polyphenols - isolation & purification</topic><topic>Polyphenols - pharmacology</topic><topic>RAW 264.7 Cells</topic><topic>RNA, Messenger - analysis</topic><topic>Signal Transduction - drug effects</topic><topic>Signalling</topic><topic>Solubility</topic><topic>Transcription Factor RelA - metabolism</topic><topic>Vaccinium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, Anwei</creatorcontrib><creatorcontrib>Han, Caijing</creatorcontrib><creatorcontrib>Fang, Xixiu</creatorcontrib><creatorcontrib>Sun, Jinyue</creatorcontrib><creatorcontrib>Chen, Xiangyan</creatorcontrib><creatorcontrib>Wan, Fachun</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Anwei</au><au>Han, Caijing</au><au>Fang, Xixiu</au><au>Sun, Jinyue</au><au>Chen, Xiangyan</au><au>Wan, Fachun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extractable and non-extractable polyphenols from blueberries modulate LPS-induced expression of iNOS and COX-2 in RAW264.7 macrophages via the NF-κB signalling pathway</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J. Sci. Food Agric</addtitle><date>2016-08-01</date><risdate>2016</risdate><volume>96</volume><issue>10</issue><spage>3393</spage><epage>3400</epage><pages>3393-3400</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><abstract>BACKGROUND
Plant polyphenols are rich in blueberries that have a wide range of properties beneficial to human health. There are two types, according to the solubility of polyphenols, which were defined as extractable polyphenols (EPP) and non‐extractable polyphenols (NEPP), respectively. At present, in most of reports, ‘total polyphenol’ refers only to EPP excluding NEPP. In this paper, the effects of EPP and NEPP on lipopolysaccharides (LPS) induced production of nitric oxide (NO) and gene expression of inducible NO synthase (iNOS) and cyclooxygenase‐2 (COX‐2) in RAW264.7 cells via nuclear factor‐κB (NF‐κB) signalling pathway were compared.
RESULTS
The results showed that EPP and NEPP from blueberries significantly inhibited the LPS‐induced production of NO and gene expression of iNOS and COX‐2 in cells. The constitutive level of p65 sub‐unit of NF‐κB was obviously detected after the treatments with EPP or NEPP. By contrast, the level of phosphorylated p65 (P‐p65) was strongly inhibited by EPP or NEPP. EPP had a stronger inhibition on the gene expression of iNOS and COX‐2 than that of NEPP.
CONCLUSION
These findings of inhibition of iNOS and COX‐2 mRNA expression through the suppression of NF‐κB suggest that EPP and ENPP from blueberries have significant anti‐inflammatory effect and may be a potential medicine. © 2015 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>26538333</pmid><doi>10.1002/jsfa.7519</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Anti-Inflammatory Agents Blueberries Blueberry Plants Cyclooxygenase 2 - genetics cyclooxygenase-2 extractable polyphenols Foods Fruit - chemistry Gene expression Gene Expression - drug effects inducible nitric oxide synthase Inhibition Lipopolysaccharides - pharmacology Macrophages - drug effects Macrophages - metabolism Mice NF-kappa B - antagonists & inhibitors NF-kappa B - metabolism Nitric Oxide Synthase Type II - genetics non-extractable polyphenols nuclear factor-κB Pathways Phosphorylation - drug effects Polyphenols Polyphenols - isolation & purification Polyphenols - pharmacology RAW 264.7 Cells RNA, Messenger - analysis Signal Transduction - drug effects Signalling Solubility Transcription Factor RelA - metabolism Vaccinium |
| title | Extractable and non-extractable polyphenols from blueberries modulate LPS-induced expression of iNOS and COX-2 in RAW264.7 macrophages via the NF-κB signalling pathway |
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