Protein Based Localized Surface Plasmon Resonance Gas Sensing
We apply the localized surface plasrnon resonance （LSPR） of gold nanoparticles （GNPs） covalently coupled with cytochrorne c （cyt c） to create a nanobiosensor for detecting hydrogen sulfide （H2S） in the range of 15 lOOppb. Monolayer formation of GNPs on glass surface functionalized with 3-aminopropyl...
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| Veröffentlicht in: | Chinese physics letters 2015, Vol.32 (1), p.166-169, Article 018701 |
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| creator | Omidi, Meisam Amoabediny, Gh Yazdian, F. Habibi-Rezaei, M. |
| description | We apply the localized surface plasrnon resonance （LSPR） of gold nanoparticles （GNPs） covalently coupled with cytochrorne c （cyt c） to create a nanobiosensor for detecting hydrogen sulfide （H2S） in the range of 15 lOOppb. Monolayer formation of GNPs on glass surface functionalized with 3-aminopropyltrirnethoxysilane （APTMS） is performed for fabricating a chip-based format of the optical transducer. By chemical introduction of short-chain thiol derivatives on cyt c protein shell via its lysine residues, a very fast self-assembled rnonolayer （SAM） of cyt c is formed on the GNPs. Significant shifts in the LSPR peak （△λLSPR） are observed by reacting H2S with cyt c. Results show a linear relationship between △λLSPR and H2S concentration. Furthermore, shifts in the LSPR peak are reversible and the peak positions return to their pre-exposure values once the H2S is removed. The experirnental results strongly indicate that the protein based LSPR chip can be successfully used as a simple, fast, sensitive and quantitative sensor for H2S detection. |
| doi_str_mv | 10.1088/0256-307X/32/1/018701 |
| format | Article |
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Monolayer formation of GNPs on glass surface functionalized with 3-aminopropyltrirnethoxysilane （APTMS） is performed for fabricating a chip-based format of the optical transducer. By chemical introduction of short-chain thiol derivatives on cyt c protein shell via its lysine residues, a very fast self-assembled rnonolayer （SAM） of cyt c is formed on the GNPs. Significant shifts in the LSPR peak （△λLSPR） are observed by reacting H2S with cyt c. Results show a linear relationship between △λLSPR and H2S concentration. Furthermore, shifts in the LSPR peak are reversible and the peak positions return to their pre-exposure values once the H2S is removed. 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Monolayer formation of GNPs on glass surface functionalized with 3-aminopropyltrirnethoxysilane （APTMS） is performed for fabricating a chip-based format of the optical transducer. By chemical introduction of short-chain thiol derivatives on cyt c protein shell via its lysine residues, a very fast self-assembled rnonolayer （SAM） of cyt c is formed on the GNPs. Significant shifts in the LSPR peak （△λLSPR） are observed by reacting H2S with cyt c. Results show a linear relationship between △λLSPR and H2S concentration. Furthermore, shifts in the LSPR peak are reversible and the peak positions return to their pre-exposure values once the H2S is removed. 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| fulltext | fulltext |
| identifier | ISSN: 0256-307X |
| ispartof | Chinese physics letters, 2015, Vol.32 (1), p.166-169, Article 018701 |
| issn | 0256-307X 1741-3540 1741-3540 |
| language | eng |
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| source | Institute of Physics Journals; Alma/SFX Local Collection |
| subjects | Deltas Derivatives Format Glass Nanostructure Plasmons Proteins Transducers 变换装置 国民生产总值 气敏 生物传感器 硫化氢检测 蛋白质 表面等离子体共振 金纳米粒子 |
| title | Protein Based Localized Surface Plasmon Resonance Gas Sensing |
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