Effects of acute exercise on the gluconeogenic capacity of periportal and perivenous hepatocytes

1 Département de Kinésiologie and 2 Département de Pharmacologie, Université de Montréal, Montréal, Québec, Canada H3C 3J7 The present study was conducted to examine the effect of a single bout of exercise (rodent treadmill, 60 min at 26 m/min, 0% grade) on the gluconeogenic activity of periportal hepatocytes (PP-H) and perivenous hepatocytes (PV-H) in fasted (18 h) rats. Isolated PP-H and PV-H, obtained by selective destruction following liver perfusion with digitonin and collagenase, were incubated with saturating concentrations of alanine (Ala; 20 mM) or a mixture of lactate and pyruvate (Lac+Pyr; 20:2 mM) to determine the glucose production flux ( J glucose ) in the incubation medium. Results show that, in the resting conditions, J glucose from all exogenous substrates was significantly higher ( P