Detection of Clostridium botulinum neurotoxin type A using immuno‐PCR

Aims: An immuno‐polymerase chain reaction (immuno‐PCR) has been developed for the sensitive detection of antigens, which greatly extends the detection limits of immunoassays. In the current study, the method was applied to the detection of Clostridium botulinum neurotoxin type A (BTx‐A). Methods and...

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Veröffentlicht in:	Letters in applied microbiology 2001-05, Vol.32 (5), p.321-325
Hauptverfasser:	WU, H. C, HUANG, Y. L, LAI, S. C, HUANG, Y. Y, SHAIO, M. F
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - immunology Bacterial Proteins - isolation & purification Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Biotechnology Botulinum Toxins, Type A - immunology Botulinum Toxins, Type A - isolation & purification Botulinum Toxins, Type A - toxicity Clostridium botulinum Clostridium botulinum - enzymology DNA Primers - genetics Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Immunological technics applied to diagnosis Industrial applications and implications. Economical aspects Microbiology neurotoxin A Polymerase Chain Reaction - methods Sensitivity and Specificity
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