Paradoxical performance of tryptophan synthase gene trp1+ in transformations of the basidiomycete Coprinopsis cinerea

Several transformation strains of Coprinopsis cinerea carry the defective tryptophan synthase allele trp1-1 , 1-6 which can be complemented by introduction of the trp1 + wild-type gene. Regularly in C. cinerea , single- trp1 + -vector transformations yield about half the numbers of clones than cotra...

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Veröffentlicht in:	Applied microbiology and biotechnology 2016-10, Vol.100 (20), p.8789-8807
Hauptverfasser:	Dörnte, Bastian, Kües, Ursula
Format:	Artikel
Sprache:	eng
Schlagworte:	Agaricales - enzymology Agaricales - genetics Agaricales - metabolism Applied Genetics and Molecular Biotechnology Biomedical and Life Sciences Biotechnology Genetic Complementation Test Homologous Recombination Life Sciences Microbial Genetics and Genomics Microbiology Plasmids Transformation, Genetic Tryptophan Synthase - genetics Tryptophan Synthase - metabolism
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