Inhibitory effect of myb7 uORF on downstream gene expression in homologous (rice) and heterologous (tobacco) systems
The rice myb7 mRNA contains in its long leader an upstream open reading frame (uORF) putatively coding for a 40 amino acid peptide. uORFs have been found in the leader of mRNAs encoding transcriptional factors or other proteins involved in cellular growth and development. They are thought to transla...
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| Veröffentlicht in: | Plant molecular biology 2002-02, Vol.48 (3), p.309-318 |
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| creator | Locatelli, Franca Magnani, Enrico Vighi, Cristina Lanzanova, Chiara Coraggio, Immacolata |
| description | The rice myb7 mRNA contains in its long leader an upstream open reading frame (uORF) putatively coding for a 40 amino acid peptide. uORFs have been found in the leader of mRNAs encoding transcriptional factors or other proteins involved in cellular growth and development. They are thought to translationally regulate the expression of downstream ORFs. Here, we showed the ability of the myb7 uORF to inhibit the expression of downstream reporter genes both in homologous (rice) and heterologous (tobacco) systems. This effect seems partially related to its translation, as indicated by the comparison with the mutagenized uORF. In both systems most of the inhibitory effect was due to the presence of the intercistronic region, in disagreement with the Kozak model. Moreover, replacing the uORF or the intercistronic region with a different one, we demonstrated that the inhibitory effect strictly depends on their co-presence. Finally, in vitro assays showed that the myb7 uORF is translated and inhibits the downstream ORF translation. |
| doi_str_mv | 10.1023/A:1013340004348 |
| format | Article |
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They are thought to translationally regulate the expression of downstream ORFs. Here, we showed the ability of the myb7 uORF to inhibit the expression of downstream reporter genes both in homologous (rice) and heterologous (tobacco) systems. This effect seems partially related to its translation, as indicated by the comparison with the mutagenized uORF. In both systems most of the inhibitory effect was due to the presence of the intercistronic region, in disagreement with the Kozak model. Moreover, replacing the uORF or the intercistronic region with a different one, we demonstrated that the inhibitory effect strictly depends on their co-presence. Finally, in vitro assays showed that the myb7 uORF is translated and inhibits the downstream ORF translation.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1023/A:1013340004348</identifier><identifier>PMID: 11855732</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Amino acids ; Bacteria ; Chloramphenicol O-Acetyltransferase - genetics ; Chloramphenicol O-Acetyltransferase - metabolism ; DNA-Binding Proteins - genetics ; Gene expression ; Gene Expression Regulation, Plant ; Glucuronidase - genetics ; Glucuronidase - metabolism ; myb7 gene ; Nicotiana - genetics ; Nicotiana tabacum ; Open Reading Frames - genetics ; Oryza - genetics ; Oryza sativa ; Plant Proteins - genetics ; Protein Biosynthesis ; Proto-Oncogene Proteins c-myb ; Protoplasts - metabolism ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism</subject><ispartof>Plant molecular biology, 2002-02, Vol.48 (3), p.309-318</ispartof><rights>Kluwer Academic Publishers 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c311t-24866f3fa2e917f96b7afd2f6aff3e646a8812a1e710ee9d9fd05a68088336403</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11855732$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Locatelli, Franca</creatorcontrib><creatorcontrib>Magnani, Enrico</creatorcontrib><creatorcontrib>Vighi, Cristina</creatorcontrib><creatorcontrib>Lanzanova, Chiara</creatorcontrib><creatorcontrib>Coraggio, Immacolata</creatorcontrib><title>Inhibitory effect of myb7 uORF on downstream gene expression in homologous (rice) and heterologous (tobacco) systems</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>The rice myb7 mRNA contains in its long leader an upstream open reading frame (uORF) putatively coding for a 40 amino acid peptide. uORFs have been found in the leader of mRNAs encoding transcriptional factors or other proteins involved in cellular growth and development. They are thought to translationally regulate the expression of downstream ORFs. Here, we showed the ability of the myb7 uORF to inhibit the expression of downstream reporter genes both in homologous (rice) and heterologous (tobacco) systems. This effect seems partially related to its translation, as indicated by the comparison with the mutagenized uORF. In both systems most of the inhibitory effect was due to the presence of the intercistronic region, in disagreement with the Kozak model. Moreover, replacing the uORF or the intercistronic region with a different one, we demonstrated that the inhibitory effect strictly depends on their co-presence. Finally, in vitro assays showed that the myb7 uORF is translated and inhibits the downstream ORF translation.</description><subject>Amino acids</subject><subject>Bacteria</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Glucuronidase - genetics</subject><subject>Glucuronidase - metabolism</subject><subject>myb7 gene</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana tabacum</subject><subject>Open Reading Frames - genetics</subject><subject>Oryza - genetics</subject><subject>Oryza sativa</subject><subject>Plant Proteins - genetics</subject><subject>Protein Biosynthesis</subject><subject>Proto-Oncogene Proteins c-myb</subject><subject>Protoplasts - metabolism</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - 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genetics</topic><topic>Chloramphenicol O-Acetyltransferase - metabolism</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Glucuronidase - genetics</topic><topic>Glucuronidase - metabolism</topic><topic>myb7 gene</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana tabacum</topic><topic>Open Reading Frames - genetics</topic><topic>Oryza - genetics</topic><topic>Oryza sativa</topic><topic>Plant Proteins - genetics</topic><topic>Protein Biosynthesis</topic><topic>Proto-Oncogene Proteins c-myb</topic><topic>Protoplasts - metabolism</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Locatelli, Franca</creatorcontrib><creatorcontrib>Magnani, Enrico</creatorcontrib><creatorcontrib>Vighi, Cristina</creatorcontrib><creatorcontrib>Lanzanova, Chiara</creatorcontrib><creatorcontrib>Coraggio, Immacolata</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Locatelli, Franca</au><au>Magnani, Enrico</au><au>Vighi, Cristina</au><au>Lanzanova, Chiara</au><au>Coraggio, Immacolata</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effect of myb7 uORF on downstream gene expression in homologous (rice) and heterologous (tobacco) systems</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>2002-02-01</date><risdate>2002</risdate><volume>48</volume><issue>3</issue><spage>309</spage><epage>318</epage><pages>309-318</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>The rice myb7 mRNA contains in its long leader an upstream open reading frame (uORF) putatively coding for a 40 amino acid peptide. uORFs have been found in the leader of mRNAs encoding transcriptional factors or other proteins involved in cellular growth and development. They are thought to translationally regulate the expression of downstream ORFs. Here, we showed the ability of the myb7 uORF to inhibit the expression of downstream reporter genes both in homologous (rice) and heterologous (tobacco) systems. This effect seems partially related to its translation, as indicated by the comparison with the mutagenized uORF. In both systems most of the inhibitory effect was due to the presence of the intercistronic region, in disagreement with the Kozak model. Moreover, replacing the uORF or the intercistronic region with a different one, we demonstrated that the inhibitory effect strictly depends on their co-presence. Finally, in vitro assays showed that the myb7 uORF is translated and inhibits the downstream ORF translation.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>11855732</pmid><doi>10.1023/A:1013340004348</doi><tpages>10</tpages></addata></record> |
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| ispartof | Plant molecular biology, 2002-02, Vol.48 (3), p.309-318 |
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| subjects | Amino acids Bacteria Chloramphenicol O-Acetyltransferase - genetics Chloramphenicol O-Acetyltransferase - metabolism DNA-Binding Proteins - genetics Gene expression Gene Expression Regulation, Plant Glucuronidase - genetics Glucuronidase - metabolism myb7 gene Nicotiana - genetics Nicotiana tabacum Open Reading Frames - genetics Oryza - genetics Oryza sativa Plant Proteins - genetics Protein Biosynthesis Proto-Oncogene Proteins c-myb Protoplasts - metabolism Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism |
| title | Inhibitory effect of myb7 uORF on downstream gene expression in homologous (rice) and heterologous (tobacco) systems |
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