Suppressed recombination around the MXC3 locus, a major gene for resistance to poplar leaf rust
A positional cloning strategy is being implemented in Populus for the isolation of the dominant MXC3 allele, which confers resistance to poplar leaf rust caused by Melampsora×columbiana (pathotype 3). AFLP markers were used to saturate the chromosomal region around the MXC3 locus in a large (n=1,902...
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| Veröffentlicht in: | Theoretical and applied genetics 2001-12, Vol.103 (8), p.1129-1137 |
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| container_title | Theoretical and applied genetics |
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| creator | STIRLING, B NEWCOMBE, G VREBALOV, J BOSDET, I BRADSHAW, H. D |
| description | A positional cloning strategy is being implemented in Populus for the isolation of the dominant MXC3 allele, which confers resistance to poplar leaf rust caused by Melampsora×columbiana (pathotype 3). AFLP markers were used to saturate the chromosomal region around the MXC3 locus in a large (n=1,902) Populus trichocarpa×P. deltoides (T×D) mapping pedigree segregating 1:1 for rust resistance and susceptibility. The high-resolution linkage map developed around the MXC3 locus contains 19 AFLP markers and spans a genetic distance of 2.73 cM. Of the 19 AFLP markers, seven were found to co-segregate with the locus. One co-segregating AFLP marker, CCG.GCT_01, was converted to an STS marker (BVS1) and used to identify a physical contig of overlapping BAC clones from the MXC3 region. Genetic and physical mapping of markers isolated from the BAC contig failed to delimit the MXC3 locus within a 300-kb interval defined by the overlapping BAC clones. This result indicates a >25-fold reduction in recombination frequency in the MXC3 region compared to the average rate of recombination for the Populus genome.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1007/s001220100721 |
| format | Article |
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D</creator><creatorcontrib>STIRLING, B ; NEWCOMBE, G ; VREBALOV, J ; BOSDET, I ; BRADSHAW, H. D</creatorcontrib><description>A positional cloning strategy is being implemented in Populus for the isolation of the dominant MXC3 allele, which confers resistance to poplar leaf rust caused by Melampsora×columbiana (pathotype 3). AFLP markers were used to saturate the chromosomal region around the MXC3 locus in a large (n=1,902) Populus trichocarpa×P. deltoides (T×D) mapping pedigree segregating 1:1 for rust resistance and susceptibility. The high-resolution linkage map developed around the MXC3 locus contains 19 AFLP markers and spans a genetic distance of 2.73 cM. Of the 19 AFLP markers, seven were found to co-segregate with the locus. One co-segregating AFLP marker, CCG.GCT_01, was converted to an STS marker (BVS1) and used to identify a physical contig of overlapping BAC clones from the MXC3 region. Genetic and physical mapping of markers isolated from the BAC contig failed to delimit the MXC3 locus within a 300-kb interval defined by the overlapping BAC clones. This result indicates a >25-fold reduction in recombination frequency in the MXC3 region compared to the average rate of recombination for the Populus genome.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s001220100721</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Agronomy. Soil science and plant productions ; Bacterial artificial chromosomes ; Biological and medical sciences ; Cloning ; Fundamental and applied biological sciences. 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D</creatorcontrib><title>Suppressed recombination around the MXC3 locus, a major gene for resistance to poplar leaf rust</title><title>Theoretical and applied genetics</title><description>A positional cloning strategy is being implemented in Populus for the isolation of the dominant MXC3 allele, which confers resistance to poplar leaf rust caused by Melampsora×columbiana (pathotype 3). AFLP markers were used to saturate the chromosomal region around the MXC3 locus in a large (n=1,902) Populus trichocarpa×P. deltoides (T×D) mapping pedigree segregating 1:1 for rust resistance and susceptibility. The high-resolution linkage map developed around the MXC3 locus contains 19 AFLP markers and spans a genetic distance of 2.73 cM. Of the 19 AFLP markers, seven were found to co-segregate with the locus. One co-segregating AFLP marker, CCG.GCT_01, was converted to an STS marker (BVS1) and used to identify a physical contig of overlapping BAC clones from the MXC3 region. Genetic and physical mapping of markers isolated from the BAC contig failed to delimit the MXC3 locus within a 300-kb interval defined by the overlapping BAC clones. This result indicates a >25-fold reduction in recombination frequency in the MXC3 region compared to the average rate of recombination for the Populus genome.[PUBLICATION ABSTRACT]</description><subject>Agronomy. Soil science and plant productions</subject><subject>Bacterial artificial chromosomes</subject><subject>Biological and medical sciences</subject><subject>Cloning</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics and breeding of economic plants</subject><subject>Genomes</subject><subject>Infections</subject><subject>Leaves</subject><subject>Medical research</subject><subject>Melamspora columbiana</subject><subject>Molecular and cellular biology</subject><subject>MXC3 gene</subject><subject>Pathogens</subject><subject>Pest resistance</subject><subject>Plant diseases</subject><subject>Plant pathogens</subject><subject>Populus deltoides</subject><subject>Populus trichocarpa</subject><subject>Varietal selection. 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Specialized plant breeding, plant breeding aims</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>STIRLING, B</creatorcontrib><creatorcontrib>NEWCOMBE, G</creatorcontrib><creatorcontrib>VREBALOV, J</creatorcontrib><creatorcontrib>BOSDET, I</creatorcontrib><creatorcontrib>BRADSHAW, H. 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| ispartof | Theoretical and applied genetics, 2001-12, Vol.103 (8), p.1129-1137 |
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| subjects | Agronomy. Soil science and plant productions Bacterial artificial chromosomes Biological and medical sciences Cloning Fundamental and applied biological sciences. Psychology Genetics and breeding of economic plants Genomes Infections Leaves Medical research Melamspora columbiana Molecular and cellular biology MXC3 gene Pathogens Pest resistance Plant diseases Plant pathogens Populus deltoides Populus trichocarpa Varietal selection. Specialized plant breeding, plant breeding aims |
| title | Suppressed recombination around the MXC3 locus, a major gene for resistance to poplar leaf rust |
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