Flow cytometry‐based TCR‐ligand Koff‐rate assay for fast avidity screening of even very small antigen‐specific T cell populations ex vivo

High epitope‐specific sensitivity of CD8+ T cells is required for optimal immune protection against intracellular pathogens as well as certain malignancies. The quality of antigen recognition of CD8+ T cells is usually described as “avidity” to its cognate peptide MHCI complex. T cell avidity is mai...

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Veröffentlicht in:	Cytometry. Part A 2016-09, Vol.89 (9), p.816-825
Hauptverfasser:	Nauerth, Magdalena, Stemberger, Christian, Mohr, Fabian, Weißbrich, Bianca, Schiemann, Matthias, Germeroth, Lothar, Busch, Dirk H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens - immunology CD8-Positive T-Lymphocytes - immunology direct avidity screening dissociation kinetics Epitopes, T-Lymphocyte - immunology Flow Cytometry - methods Humans Ligands Lymphocyte Activation - immunology MHC multimer double staining Mice online injection Receptors, Antigen, T-Cell - immunology structural TCR avidity T cell functionality T cell therapy T-Lymphocytes - immunology TCR‐ligand‐koff‐rate temperature controlled analysis uninterrupted acquisition
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