Biophysical characterization of the domain association between cytosolic A and B domains of the mannitol transporter enzymes II(Mtl) in the presence and absence of a connecting linker

The mannitol transporter enzyme II(Mtl) of the bacterial phosphotransferase system is a multi-domain protein that catalyzes mannitol uptake and phosphorylation. Here we investigated the domain association between cytosolic A and B domains of enzyme II(Mtl) , which are natively connected in Escherich...

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Veröffentlicht in:	Protein science 2016-10, Vol.25 (10), p.1803-1811
Hauptverfasser:	Lee, Ko On, Kim, Eun-Hee, Kim, Gowoon, Jung, Jea Yeon, Katayama, Shigeru, Nakamura, Soichiro, Suh, Jeong-Yong
Format:	Artikel
Sprache:	eng
Schlagworte:	Carrier Proteins - chemistry Carrier Proteins - genetics Carrier Proteins - metabolism Cytosol - chemistry Cytosol - metabolism Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli Proteins - chemistry Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Protein Domains Thermoanaerobacter - enzymology Thermoanaerobacter - genetics
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