Seminal plasma transforming growth factor-β, activin A and follistatin fluctuate within men over time
Abstract STUDY QUESTION Do seminal plasma transforming growth factor-β (TGFB) cytokines vary within individuals over time, and does this relate to sperm parameters, age or prior abstinence? SUMMARY ANSWER Activin A and follistatin, and to a lesser extent TGFB1, TGFB2 and TGFB3, vary within individua...
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| Veröffentlicht in: | Human reproduction (Oxford) 2016-10, Vol.31 (10), p.2183-2191 |
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| creator | Sharkey, David J. Tremellen, Kelton P. Briggs, Nancy E. Dekker, Gustaaf A. Robertson, Sarah A. |
| description | Abstract
STUDY QUESTION
Do seminal plasma transforming growth factor-β (TGFB) cytokines vary within individuals over time, and does this relate to sperm parameters, age or prior abstinence?
SUMMARY ANSWER
Activin A and follistatin, and to a lesser extent TGFB1, TGFB2 and TGFB3, vary within individuals over time, in association with duration of abstinence.
WHAT IS ALREADY KNOWN
Seminal plasma TGFB cytokines can influence sperm function and reproductive success through interactions with the female reproductive tract after coitus. Over time, individual sperm parameters fluctuate considerably. Whether seminal fluid TGFB cytokines vary similarly, and the determinants of any variance, is unknown.
STUDY DESIGN, SIZE, DURATION
Between two and seven semen samples were collected from each of 14 fertile donors at 6–10 week intervals over the course of 12 months, then seminal plasma cytokines and sperm parameters were measured.
PARTICIPANTS/MATERIALS, SETTING AND METHOD
The concentrations and total amounts per ejaculate of TGFB1, TGFB2, TGFB3, activin A and follistatin were determined using commercial assays. Sperm parameters were assessed according to WHO IV standards. Mixed model analysis was utilised to determine the relative contribution of between- and within-individual factors to the variance. Relationships between cytokines and sperm parameters, as well as effect of age and duration of abstinence, were investigated by correlation analysis.
MAIN RESULTS AND THE ROLE OF CHANCE
Within-individual variability contributed to the total variance for all cytokines and sperm parameters, and was a stronger determinant than between-individual variability for activin A and follistatin as well as for total sperm concentration and sperm motility. Positive correlations between each of the three TGFB isoforms, and activin and follistatin, suggest co-regulation of synthesis. Duration of abstinence influenced total content of TGFB1, TGFB2, activin A and follistatin. TGFB1 correlated inversely with age.
LIMITATIONS, REASONS FOR CAUTION
A limited number of donors from a single clinic were investigated. Clinical information on BMI, nutrition, smoking and other lifestyle factors was unavailable. Further studies are required to determine whether the findings can be generalised to larger populations and different ethnicities.
WIDER IMPLICATIONS OF THE FINDINGS
These data reveal substantial variation over time in seminal fluid cytokines and indicate that repeated analyses are required t |
| doi_str_mv | 10.1093/humrep/dew185 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1822115387</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/humrep/dew185</oup_id><sourcerecordid>1822115387</sourcerecordid><originalsourceid>FETCH-LOGICAL-c326t-7053f6ba506de01e2e6613080ebd99dcba5a722dbfc29043c78a632cfb4bb7503</originalsourceid><addsrcrecordid>eNqFkMtOwzAQRS0EoqWwZIu8ZEGoH42TLKuKl1SJBbCOHGfcBtlxsJ1W_BYfwjcR1AJLVjO6c3RHOgidU3JNScGn69566KY1bGmeHqAxnQmSMJ6SQzQmTOQJpYKO0EkIr4QMay6O0YhlghRFno6RfgLbtNLgzshgJY5etkE7P4QrvPJuG9dYSxWdTz4_rvCwNZumxXMs2xprZ0wTooxDok2vYi8j4G0T10NgocVuAx7HxsIpOtLSBDjbzwl6ub15Xtwny8e7h8V8mSjOREwyknItKpkSUQOhwEAIyklOoKqLolbDRWaM1ZVWrCAzrrJcCs6UrmZVlaWET9Dlrrfz7q2HEEvbBAXGyBZcH0qaM0ZpyvNsQJMdqrwLwYMuO99Y6d9LSspvteVObblTO_AX--q-slD_0j8u_367vvun6wt0aoce</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1822115387</pqid></control><display><type>article</type><title>Seminal plasma transforming growth factor-β, activin A and follistatin fluctuate within men over time</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Sharkey, David J. ; Tremellen, Kelton P. ; Briggs, Nancy E. ; Dekker, Gustaaf A. ; Robertson, Sarah A.</creator><creatorcontrib>Sharkey, David J. ; Tremellen, Kelton P. ; Briggs, Nancy E. ; Dekker, Gustaaf A. ; Robertson, Sarah A.</creatorcontrib><description>Abstract
STUDY QUESTION
Do seminal plasma transforming growth factor-β (TGFB) cytokines vary within individuals over time, and does this relate to sperm parameters, age or prior abstinence?
SUMMARY ANSWER
Activin A and follistatin, and to a lesser extent TGFB1, TGFB2 and TGFB3, vary within individuals over time, in association with duration of abstinence.
WHAT IS ALREADY KNOWN
Seminal plasma TGFB cytokines can influence sperm function and reproductive success through interactions with the female reproductive tract after coitus. Over time, individual sperm parameters fluctuate considerably. Whether seminal fluid TGFB cytokines vary similarly, and the determinants of any variance, is unknown.
STUDY DESIGN, SIZE, DURATION
Between two and seven semen samples were collected from each of 14 fertile donors at 6–10 week intervals over the course of 12 months, then seminal plasma cytokines and sperm parameters were measured.
PARTICIPANTS/MATERIALS, SETTING AND METHOD
The concentrations and total amounts per ejaculate of TGFB1, TGFB2, TGFB3, activin A and follistatin were determined using commercial assays. Sperm parameters were assessed according to WHO IV standards. Mixed model analysis was utilised to determine the relative contribution of between- and within-individual factors to the variance. Relationships between cytokines and sperm parameters, as well as effect of age and duration of abstinence, were investigated by correlation analysis.
MAIN RESULTS AND THE ROLE OF CHANCE
Within-individual variability contributed to the total variance for all cytokines and sperm parameters, and was a stronger determinant than between-individual variability for activin A and follistatin as well as for total sperm concentration and sperm motility. Positive correlations between each of the three TGFB isoforms, and activin and follistatin, suggest co-regulation of synthesis. Duration of abstinence influenced total content of TGFB1, TGFB2, activin A and follistatin. TGFB1 correlated inversely with age.
LIMITATIONS, REASONS FOR CAUTION
A limited number of donors from a single clinic were investigated. Clinical information on BMI, nutrition, smoking and other lifestyle factors was unavailable. Further studies are required to determine whether the findings can be generalised to larger populations and different ethnicities.
WIDER IMPLICATIONS OF THE FINDINGS
These data reveal substantial variation over time in seminal fluid cytokines and indicate that repeated analyses are required to gain precise representative data on an individual's status. Within-individual variation in seminal fluid components should be taken into account when investigating seminal fluid cytokines.
STUDY FUNDING/COMPETING INTERESTS
This study was supported by grants from the National Health and Medical Research Council of Australia, ID453556 and APP1041332. The authors have no competing interests to disclose.</description><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/dew185</identifier><identifier>PMID: 27609985</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Activins - analysis ; Adolescent ; Adult ; Age Factors ; Aging - physiology ; Follistatin - analysis ; Humans ; Male ; Middle Aged ; Semen - chemistry ; Semen Analysis ; Sperm Count ; Transforming Growth Factor beta - analysis ; Young Adult</subject><ispartof>Human reproduction (Oxford), 2016-10, Vol.31 (10), p.2183-2191</ispartof><rights>The Author 2016. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.For Permissions, please email: journals.permissions@oup.com 2016</rights><rights>The Author 2016. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.For Permissions, please email: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c326t-7053f6ba506de01e2e6613080ebd99dcba5a722dbfc29043c78a632cfb4bb7503</citedby><cites>FETCH-LOGICAL-c326t-7053f6ba506de01e2e6613080ebd99dcba5a722dbfc29043c78a632cfb4bb7503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27609985$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sharkey, David J.</creatorcontrib><creatorcontrib>Tremellen, Kelton P.</creatorcontrib><creatorcontrib>Briggs, Nancy E.</creatorcontrib><creatorcontrib>Dekker, Gustaaf A.</creatorcontrib><creatorcontrib>Robertson, Sarah A.</creatorcontrib><title>Seminal plasma transforming growth factor-β, activin A and follistatin fluctuate within men over time</title><title>Human reproduction (Oxford)</title><addtitle>Hum Reprod</addtitle><description>Abstract
STUDY QUESTION
Do seminal plasma transforming growth factor-β (TGFB) cytokines vary within individuals over time, and does this relate to sperm parameters, age or prior abstinence?
SUMMARY ANSWER
Activin A and follistatin, and to a lesser extent TGFB1, TGFB2 and TGFB3, vary within individuals over time, in association with duration of abstinence.
WHAT IS ALREADY KNOWN
Seminal plasma TGFB cytokines can influence sperm function and reproductive success through interactions with the female reproductive tract after coitus. Over time, individual sperm parameters fluctuate considerably. Whether seminal fluid TGFB cytokines vary similarly, and the determinants of any variance, is unknown.
STUDY DESIGN, SIZE, DURATION
Between two and seven semen samples were collected from each of 14 fertile donors at 6–10 week intervals over the course of 12 months, then seminal plasma cytokines and sperm parameters were measured.
PARTICIPANTS/MATERIALS, SETTING AND METHOD
The concentrations and total amounts per ejaculate of TGFB1, TGFB2, TGFB3, activin A and follistatin were determined using commercial assays. Sperm parameters were assessed according to WHO IV standards. Mixed model analysis was utilised to determine the relative contribution of between- and within-individual factors to the variance. Relationships between cytokines and sperm parameters, as well as effect of age and duration of abstinence, were investigated by correlation analysis.
MAIN RESULTS AND THE ROLE OF CHANCE
Within-individual variability contributed to the total variance for all cytokines and sperm parameters, and was a stronger determinant than between-individual variability for activin A and follistatin as well as for total sperm concentration and sperm motility. Positive correlations between each of the three TGFB isoforms, and activin and follistatin, suggest co-regulation of synthesis. Duration of abstinence influenced total content of TGFB1, TGFB2, activin A and follistatin. TGFB1 correlated inversely with age.
LIMITATIONS, REASONS FOR CAUTION
A limited number of donors from a single clinic were investigated. Clinical information on BMI, nutrition, smoking and other lifestyle factors was unavailable. Further studies are required to determine whether the findings can be generalised to larger populations and different ethnicities.
WIDER IMPLICATIONS OF THE FINDINGS
These data reveal substantial variation over time in seminal fluid cytokines and indicate that repeated analyses are required to gain precise representative data on an individual's status. Within-individual variation in seminal fluid components should be taken into account when investigating seminal fluid cytokines.
STUDY FUNDING/COMPETING INTERESTS
This study was supported by grants from the National Health and Medical Research Council of Australia, ID453556 and APP1041332. The authors have no competing interests to disclose.</description><subject>Activins - analysis</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Age Factors</subject><subject>Aging - physiology</subject><subject>Follistatin - analysis</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Semen - chemistry</subject><subject>Semen Analysis</subject><subject>Sperm Count</subject><subject>Transforming Growth Factor beta - analysis</subject><subject>Young Adult</subject><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EoqWwZIu8ZEGoH42TLKuKl1SJBbCOHGfcBtlxsJ1W_BYfwjcR1AJLVjO6c3RHOgidU3JNScGn69566KY1bGmeHqAxnQmSMJ6SQzQmTOQJpYKO0EkIr4QMay6O0YhlghRFno6RfgLbtNLgzshgJY5etkE7P4QrvPJuG9dYSxWdTz4_rvCwNZumxXMs2xprZ0wTooxDok2vYi8j4G0T10NgocVuAx7HxsIpOtLSBDjbzwl6ub15Xtwny8e7h8V8mSjOREwyknItKpkSUQOhwEAIyklOoKqLolbDRWaM1ZVWrCAzrrJcCs6UrmZVlaWET9Dlrrfz7q2HEEvbBAXGyBZcH0qaM0ZpyvNsQJMdqrwLwYMuO99Y6d9LSspvteVObblTO_AX--q-slD_0j8u_367vvun6wt0aoce</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Sharkey, David J.</creator><creator>Tremellen, Kelton P.</creator><creator>Briggs, Nancy E.</creator><creator>Dekker, Gustaaf A.</creator><creator>Robertson, Sarah A.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20161001</creationdate><title>Seminal plasma transforming growth factor-β, activin A and follistatin fluctuate within men over time</title><author>Sharkey, David J. ; Tremellen, Kelton P. ; Briggs, Nancy E. ; Dekker, Gustaaf A. ; Robertson, Sarah A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-7053f6ba506de01e2e6613080ebd99dcba5a722dbfc29043c78a632cfb4bb7503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Activins - analysis</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Age Factors</topic><topic>Aging - physiology</topic><topic>Follistatin - analysis</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Semen - chemistry</topic><topic>Semen Analysis</topic><topic>Sperm Count</topic><topic>Transforming Growth Factor beta - analysis</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sharkey, David J.</creatorcontrib><creatorcontrib>Tremellen, Kelton P.</creatorcontrib><creatorcontrib>Briggs, Nancy E.</creatorcontrib><creatorcontrib>Dekker, Gustaaf A.</creatorcontrib><creatorcontrib>Robertson, Sarah A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharkey, David J.</au><au>Tremellen, Kelton P.</au><au>Briggs, Nancy E.</au><au>Dekker, Gustaaf A.</au><au>Robertson, Sarah A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Seminal plasma transforming growth factor-β, activin A and follistatin fluctuate within men over time</atitle><jtitle>Human reproduction (Oxford)</jtitle><addtitle>Hum Reprod</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>31</volume><issue>10</issue><spage>2183</spage><epage>2191</epage><pages>2183-2191</pages><issn>0268-1161</issn><eissn>1460-2350</eissn><abstract>Abstract
STUDY QUESTION
Do seminal plasma transforming growth factor-β (TGFB) cytokines vary within individuals over time, and does this relate to sperm parameters, age or prior abstinence?
SUMMARY ANSWER
Activin A and follistatin, and to a lesser extent TGFB1, TGFB2 and TGFB3, vary within individuals over time, in association with duration of abstinence.
WHAT IS ALREADY KNOWN
Seminal plasma TGFB cytokines can influence sperm function and reproductive success through interactions with the female reproductive tract after coitus. Over time, individual sperm parameters fluctuate considerably. Whether seminal fluid TGFB cytokines vary similarly, and the determinants of any variance, is unknown.
STUDY DESIGN, SIZE, DURATION
Between two and seven semen samples were collected from each of 14 fertile donors at 6–10 week intervals over the course of 12 months, then seminal plasma cytokines and sperm parameters were measured.
PARTICIPANTS/MATERIALS, SETTING AND METHOD
The concentrations and total amounts per ejaculate of TGFB1, TGFB2, TGFB3, activin A and follistatin were determined using commercial assays. Sperm parameters were assessed according to WHO IV standards. Mixed model analysis was utilised to determine the relative contribution of between- and within-individual factors to the variance. Relationships between cytokines and sperm parameters, as well as effect of age and duration of abstinence, were investigated by correlation analysis.
MAIN RESULTS AND THE ROLE OF CHANCE
Within-individual variability contributed to the total variance for all cytokines and sperm parameters, and was a stronger determinant than between-individual variability for activin A and follistatin as well as for total sperm concentration and sperm motility. Positive correlations between each of the three TGFB isoforms, and activin and follistatin, suggest co-regulation of synthesis. Duration of abstinence influenced total content of TGFB1, TGFB2, activin A and follistatin. TGFB1 correlated inversely with age.
LIMITATIONS, REASONS FOR CAUTION
A limited number of donors from a single clinic were investigated. Clinical information on BMI, nutrition, smoking and other lifestyle factors was unavailable. Further studies are required to determine whether the findings can be generalised to larger populations and different ethnicities.
WIDER IMPLICATIONS OF THE FINDINGS
These data reveal substantial variation over time in seminal fluid cytokines and indicate that repeated analyses are required to gain precise representative data on an individual's status. Within-individual variation in seminal fluid components should be taken into account when investigating seminal fluid cytokines.
STUDY FUNDING/COMPETING INTERESTS
This study was supported by grants from the National Health and Medical Research Council of Australia, ID453556 and APP1041332. The authors have no competing interests to disclose.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>27609985</pmid><doi>10.1093/humrep/dew185</doi><tpages>9</tpages></addata></record> |
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| ispartof | Human reproduction (Oxford), 2016-10, Vol.31 (10), p.2183-2191 |
| issn | 0268-1161 1460-2350 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Activins - analysis Adolescent Adult Age Factors Aging - physiology Follistatin - analysis Humans Male Middle Aged Semen - chemistry Semen Analysis Sperm Count Transforming Growth Factor beta - analysis Young Adult |
| title | Seminal plasma transforming growth factor-β, activin A and follistatin fluctuate within men over time |
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