A Novel c-Myc- responsive Gene, JPO1, Participates in Neoplastic Transformation
We have identified a novel c-Myc-responsive gene, named JPO1, by representational difference analysis.JPO1 responds to two inducible c-Myc systems and behaves as a direct c-Myc target gene. JPO1 mRNA expression is readily detectable in the thymus, small intestine, and colon, whereas expression is re...
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| Veröffentlicht in: | The Journal of biological chemistry 2001-12, Vol.276 (51), p.48276-48284 |
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| container_title | The Journal of biological chemistry |
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| creator | Prescott, Julia E. Osthus, Rebecca C. Lee, Linda A. Lewis, Brian C. Shim, Hyunsuk Barrett, John F. Guo, Qingbin Hawkins, Anita L. Griffin, Constance A. Dang, Chi V. |
| description | We have identified a novel c-Myc-responsive gene, named JPO1, by representational difference analysis.JPO1 responds to two inducible c-Myc systems and behaves as a direct c-Myc target gene. JPO1 mRNA expression is readily detectable in the thymus, small intestine, and colon, whereas expression is relatively low in spleen, bone marrow, and peripheral leukocytes. We cloned a full-length JPO1 cDNA that encodes a 47-kDa nuclear protein. To determine the role of JPO1 in Myc-mediated cellular phenotypes, stable Rat1a fibroblasts overexpressing JPO1 were tested and compared with transformed Rat1a-Myc cells. Although JPO1 has a diminished transforming activity as compared with c-Myc, JPO1 complements a transformation-defective Myc Box II mutant in the Rat1a transformation assay. This complementation provides evidence for a genetic link between c-Myc and JPO1. Similar to c-Myc, JPO1 overexpression enhances the clonogenicity of CB33 human lymphoblastoid cells in methylcellulose assays. These observations suggest that JPO1 participates in c-Myc-mediated transformation, supporting an emerging concept that c-Myc target genes constitute nodal points in a network of pathways that lead from c-Myc to various Myc-related phenotypes and ultimately to tumorigenesis. |
| doi_str_mv | 10.1074/jbc.M107357200 |
| format | Article |
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JPO1 mRNA expression is readily detectable in the thymus, small intestine, and colon, whereas expression is relatively low in spleen, bone marrow, and peripheral leukocytes. We cloned a full-length JPO1 cDNA that encodes a 47-kDa nuclear protein. To determine the role of JPO1 in Myc-mediated cellular phenotypes, stable Rat1a fibroblasts overexpressing JPO1 were tested and compared with transformed Rat1a-Myc cells. Although JPO1 has a diminished transforming activity as compared with c-Myc, JPO1 complements a transformation-defective Myc Box II mutant in the Rat1a transformation assay. This complementation provides evidence for a genetic link between c-Myc and JPO1. Similar to c-Myc, JPO1 overexpression enhances the clonogenicity of CB33 human lymphoblastoid cells in methylcellulose assays. These observations suggest that JPO1 participates in c-Myc-mediated transformation, supporting an emerging concept that c-Myc target genes constitute nodal points in a network of pathways that lead from c-Myc to various Myc-related phenotypes and ultimately to tumorigenesis.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M107357200</identifier><identifier>PMID: 11598121</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; c-Myc gene ; Cell Transformation, Neoplastic - genetics ; Chromosome Mapping ; Chromosomes, Human, Pair 2 ; Cloning, Molecular ; COS Cells ; DNA, Complementary ; Genes, myc ; Genetic Complementation Test ; Humans ; JPO1 gene ; Molecular Sequence Data ; Nuclear Proteins - chemistry ; Nuclear Proteins - genetics ; Nuclear Proteins - physiology ; Rats</subject><ispartof>The Journal of biological chemistry, 2001-12, Vol.276 (51), p.48276-48284</ispartof><rights>2001 © 2001 ASBMB. 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JPO1 mRNA expression is readily detectable in the thymus, small intestine, and colon, whereas expression is relatively low in spleen, bone marrow, and peripheral leukocytes. We cloned a full-length JPO1 cDNA that encodes a 47-kDa nuclear protein. To determine the role of JPO1 in Myc-mediated cellular phenotypes, stable Rat1a fibroblasts overexpressing JPO1 were tested and compared with transformed Rat1a-Myc cells. Although JPO1 has a diminished transforming activity as compared with c-Myc, JPO1 complements a transformation-defective Myc Box II mutant in the Rat1a transformation assay. This complementation provides evidence for a genetic link between c-Myc and JPO1. Similar to c-Myc, JPO1 overexpression enhances the clonogenicity of CB33 human lymphoblastoid cells in methylcellulose assays. These observations suggest that JPO1 participates in c-Myc-mediated transformation, supporting an emerging concept that c-Myc target genes constitute nodal points in a network of pathways that lead from c-Myc to various Myc-related phenotypes and ultimately to tumorigenesis.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>c-Myc gene</subject><subject>Cell Transformation, Neoplastic - genetics</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 2</subject><subject>Cloning, Molecular</subject><subject>COS Cells</subject><subject>DNA, Complementary</subject><subject>Genes, myc</subject><subject>Genetic Complementation Test</subject><subject>Humans</subject><subject>JPO1 gene</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Proteins - chemistry</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - physiology</subject><subject>Rats</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1r3DAQhkVpaLZJrz0WHUpP8UajD1t7DCHNB0k2hxR6E7I07irYlit5t-TfV2UXcupcZhiedxgeQj4DWwJr5PlL65YPZRKq4Yy9IwtgWlRCwc_3ZMEYh2rFlT4mH3N-YaXkCj6QYwC10sBhQdYX9DHusKeuenh1FU2YpzjmsEN6jSOe0bunNZzRJ5vm4MJkZ8w0jPQR49TbXHb0OdkxdzENdg5xPCVHne0zfjr0E_Lj-9Xz5U11v76-vby4r5xo-FyhUJ2va4W1ckIwzZpa1q1srVp5LpXwrKs9iFo34DyojjMPllvVtdKLFjtxQr7t704p_t5ins0QssO-tyPGbTagOUipoYDLPehSzDlhZ6YUBpteDTDzT6EpCs2bwhL4cri8bQf0b_jBWQG-7oFN-LX5ExKaNkS3wcHwpjYKjNRlKJjeY1g07AImk13A0aEvETcbH8P_XvgLvEOJqw</recordid><startdate>20011221</startdate><enddate>20011221</enddate><creator>Prescott, Julia E.</creator><creator>Osthus, Rebecca C.</creator><creator>Lee, Linda A.</creator><creator>Lewis, Brian C.</creator><creator>Shim, Hyunsuk</creator><creator>Barrett, John F.</creator><creator>Guo, Qingbin</creator><creator>Hawkins, Anita L.</creator><creator>Griffin, Constance A.</creator><creator>Dang, Chi V.</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20011221</creationdate><title>A Novel c-Myc- responsive Gene, JPO1, Participates in Neoplastic Transformation</title><author>Prescott, Julia E. ; 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JPO1 mRNA expression is readily detectable in the thymus, small intestine, and colon, whereas expression is relatively low in spleen, bone marrow, and peripheral leukocytes. We cloned a full-length JPO1 cDNA that encodes a 47-kDa nuclear protein. To determine the role of JPO1 in Myc-mediated cellular phenotypes, stable Rat1a fibroblasts overexpressing JPO1 were tested and compared with transformed Rat1a-Myc cells. Although JPO1 has a diminished transforming activity as compared with c-Myc, JPO1 complements a transformation-defective Myc Box II mutant in the Rat1a transformation assay. This complementation provides evidence for a genetic link between c-Myc and JPO1. Similar to c-Myc, JPO1 overexpression enhances the clonogenicity of CB33 human lymphoblastoid cells in methylcellulose assays. 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| subjects | Amino Acid Sequence Animals Base Sequence c-Myc gene Cell Transformation, Neoplastic - genetics Chromosome Mapping Chromosomes, Human, Pair 2 Cloning, Molecular COS Cells DNA, Complementary Genes, myc Genetic Complementation Test Humans JPO1 gene Molecular Sequence Data Nuclear Proteins - chemistry Nuclear Proteins - genetics Nuclear Proteins - physiology Rats |
| title | A Novel c-Myc- responsive Gene, JPO1, Participates in Neoplastic Transformation |
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