Osmolality and composition of the activating solution affects motility of fresh and frozen Prochilodus lineatus sperm differently
•Activating solution osmolality affects motility of fresh and frozen prochilod sperm.•Activating solution composition affects motility of fresh but not frozen sperm.•Prochilod sperm show short period of motility which decreases fast after activation. Osmolality and composition of the activating solu...
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| Veröffentlicht in: | Animal reproduction science 2016-10, Vol.173, p.73-79 |
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| creator | Viveiros, Ana T.M. Leal, Marcelo C. França, Thales S. Almeida, Izabella L.G. Isaú, Ziara A. |
| description | •Activating solution osmolality affects motility of fresh and frozen prochilod sperm.•Activating solution composition affects motility of fresh but not frozen sperm.•Prochilod sperm show short period of motility which decreases fast after activation.
Osmolality and composition of the activating solution on motility of fresh and frozen Prochilodus lineatus sperm were evaluated. Sperm was triggered in 11 solutions prepared with reverse osmosis (RO) water (∼0mOsmkg−1), and glucose or NaCl adjusted to 50, 100, 150, 200 and 250mOsmkg−1. Sperm motility rate and velocities (curvilinear=VCL, among others) were evaluated in fresh sperm at 10, 30 and 50s post-activation (spa), and in frozen sperm at 10 spa only. Sperm was frozen under a standardized methodology for this species. Fresh sperm motility was higher in samples triggered in RO (91%), in glucose at all osmolalities (90–92%) and in 50–150mOsmkg−1 NaCl (88–91%) than that in 200–250mOsmkg−1 NaCl (74–80%). Motility decreased (P |
| doi_str_mv | 10.1016/j.anireprosci.2016.08.014 |
| format | Article |
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Osmolality and composition of the activating solution on motility of fresh and frozen Prochilodus lineatus sperm were evaluated. Sperm was triggered in 11 solutions prepared with reverse osmosis (RO) water (∼0mOsmkg−1), and glucose or NaCl adjusted to 50, 100, 150, 200 and 250mOsmkg−1. Sperm motility rate and velocities (curvilinear=VCL, among others) were evaluated in fresh sperm at 10, 30 and 50s post-activation (spa), and in frozen sperm at 10 spa only. Sperm was frozen under a standardized methodology for this species. Fresh sperm motility was higher in samples triggered in RO (91%), in glucose at all osmolalities (90–92%) and in 50–150mOsmkg−1 NaCl (88–91%) than that in 200–250mOsmkg−1 NaCl (74–80%). Motility decreased (P<0.05) as a function of time after activation in samples activated in RO and in NaCl but not in glucose. Samples activated in 100–250mOsmkg−1 glucose yielded motility above 80%, at 50 spa. Curvilinear velocity was higher (P<0.05) in glucose-activated samples (322–357μms−1) compared to that activated in NaCl (192–283μms−1) and in RO (298μms−1). Frozen sperm motility and velocities were similar when triggered in RO, glucose or NaCl and were higher at 0–150 mOsm kg−1 (69–78% motility; 163–208μms−1 VCL) than at 200–250mOsmkg−1 (34–59% motility; 127–168μms−1 VCL). High sperm motility with fast velocity for a long period is achieved at 100–150mOsmkg−1, in glucose solution for fresh sperm and in glucose or NaCl for frozen sperm.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2016.08.014</identifier><identifier>PMID: 27601320</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; CASA ; Characiformes - physiology ; Freezing ; Hydrogen-Ion Concentration ; Male ; Neotropical ; Osmolar Concentration ; Osmotic pressure ; Semen ; Semen Preservation - veterinary ; Sodium Chloride - chemistry ; Sodium Chloride - pharmacology ; Sperm Motility - drug effects ; Sperm Motility - physiology ; Spermatozoa - physiology</subject><ispartof>Animal reproduction science, 2016-10, Vol.173, p.73-79</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-a8ed96a137b24c1c339d76c41f3c1fb13181acfcc80c4de31d227993d4c758b03</citedby><cites>FETCH-LOGICAL-c447t-a8ed96a137b24c1c339d76c41f3c1fb13181acfcc80c4de31d227993d4c758b03</cites><orcidid>0000-0003-2380-9497 ; 0000-0002-5071-4971</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.anireprosci.2016.08.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27601320$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Viveiros, Ana T.M.</creatorcontrib><creatorcontrib>Leal, Marcelo C.</creatorcontrib><creatorcontrib>França, Thales S.</creatorcontrib><creatorcontrib>Almeida, Izabella L.G.</creatorcontrib><creatorcontrib>Isaú, Ziara A.</creatorcontrib><title>Osmolality and composition of the activating solution affects motility of fresh and frozen Prochilodus lineatus sperm differently</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>•Activating solution osmolality affects motility of fresh and frozen prochilod sperm.•Activating solution composition affects motility of fresh but not frozen sperm.•Prochilod sperm show short period of motility which decreases fast after activation.
Osmolality and composition of the activating solution on motility of fresh and frozen Prochilodus lineatus sperm were evaluated. Sperm was triggered in 11 solutions prepared with reverse osmosis (RO) water (∼0mOsmkg−1), and glucose or NaCl adjusted to 50, 100, 150, 200 and 250mOsmkg−1. Sperm motility rate and velocities (curvilinear=VCL, among others) were evaluated in fresh sperm at 10, 30 and 50s post-activation (spa), and in frozen sperm at 10 spa only. Sperm was frozen under a standardized methodology for this species. Fresh sperm motility was higher in samples triggered in RO (91%), in glucose at all osmolalities (90–92%) and in 50–150mOsmkg−1 NaCl (88–91%) than that in 200–250mOsmkg−1 NaCl (74–80%). Motility decreased (P<0.05) as a function of time after activation in samples activated in RO and in NaCl but not in glucose. Samples activated in 100–250mOsmkg−1 glucose yielded motility above 80%, at 50 spa. Curvilinear velocity was higher (P<0.05) in glucose-activated samples (322–357μms−1) compared to that activated in NaCl (192–283μms−1) and in RO (298μms−1). Frozen sperm motility and velocities were similar when triggered in RO, glucose or NaCl and were higher at 0–150 mOsm kg−1 (69–78% motility; 163–208μms−1 VCL) than at 200–250mOsmkg−1 (34–59% motility; 127–168μms−1 VCL). High sperm motility with fast velocity for a long period is achieved at 100–150mOsmkg−1, in glucose solution for fresh sperm and in glucose or NaCl for frozen sperm.</description><subject>Animals</subject><subject>CASA</subject><subject>Characiformes - physiology</subject><subject>Freezing</subject><subject>Hydrogen-Ion Concentration</subject><subject>Male</subject><subject>Neotropical</subject><subject>Osmolar Concentration</subject><subject>Osmotic pressure</subject><subject>Semen</subject><subject>Semen Preservation - veterinary</subject><subject>Sodium Chloride - chemistry</subject><subject>Sodium Chloride - pharmacology</subject><subject>Sperm Motility - drug effects</subject><subject>Sperm Motility - physiology</subject><subject>Spermatozoa - physiology</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUuPFCEUhYnROO3oXzC4c1Mlj7KglqbjK5lkXOia0JeLTYcqWqAmaXf-c5nu0bh0Bbmc891wDiGvOOs54-ObQ2-XkPGYU4HQizbqme4ZHx6RDddKdkJI8ZhsmFS6G6RgV-RZKQfGmBrH6Sm5EmpkvM035NdtmVO0MdQTtYujkOZjKqGGtNDkad0jtVDDna1h-U5Liuv5yXqPUAudUw1nb9P6jGV_hvicfuJCv-QE-xCTWwuNYUFb26UcMc_UhQbIuNR4ek6eeBsLvng4r8m3D--_bj91N7cfP2_f3XQwDKp2VqObRsul2okBOEg5OTXCwL0E7ndccs0teADNYHAouRNCTZN0A6i3esfkNXl94bbYfqxYqplDAYzRLpjWYrgWnE1Kc9Gk00UKLeGS0ZtjDrPNJ8OZuW_AHMw_DZj7BgzTpjXQvC8f1qy7Gd1f55_Im2B7EWD77F3AbBoCF0DXgFCNS-E_1vwGJoKheg</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Viveiros, Ana T.M.</creator><creator>Leal, Marcelo C.</creator><creator>França, Thales S.</creator><creator>Almeida, Izabella L.G.</creator><creator>Isaú, Ziara A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2380-9497</orcidid><orcidid>https://orcid.org/0000-0002-5071-4971</orcidid></search><sort><creationdate>201610</creationdate><title>Osmolality and composition of the activating solution affects motility of fresh and frozen Prochilodus lineatus sperm differently</title><author>Viveiros, Ana T.M. ; Leal, Marcelo C. ; França, Thales S. ; Almeida, Izabella L.G. ; Isaú, Ziara A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-a8ed96a137b24c1c339d76c41f3c1fb13181acfcc80c4de31d227993d4c758b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>CASA</topic><topic>Characiformes - physiology</topic><topic>Freezing</topic><topic>Hydrogen-Ion Concentration</topic><topic>Male</topic><topic>Neotropical</topic><topic>Osmolar Concentration</topic><topic>Osmotic pressure</topic><topic>Semen</topic><topic>Semen Preservation - veterinary</topic><topic>Sodium Chloride - chemistry</topic><topic>Sodium Chloride - pharmacology</topic><topic>Sperm Motility - drug effects</topic><topic>Sperm Motility - physiology</topic><topic>Spermatozoa - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Viveiros, Ana T.M.</creatorcontrib><creatorcontrib>Leal, Marcelo C.</creatorcontrib><creatorcontrib>França, Thales S.</creatorcontrib><creatorcontrib>Almeida, Izabella L.G.</creatorcontrib><creatorcontrib>Isaú, Ziara A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Viveiros, Ana T.M.</au><au>Leal, Marcelo C.</au><au>França, Thales S.</au><au>Almeida, Izabella L.G.</au><au>Isaú, Ziara A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osmolality and composition of the activating solution affects motility of fresh and frozen Prochilodus lineatus sperm differently</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2016-10</date><risdate>2016</risdate><volume>173</volume><spage>73</spage><epage>79</epage><pages>73-79</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>•Activating solution osmolality affects motility of fresh and frozen prochilod sperm.•Activating solution composition affects motility of fresh but not frozen sperm.•Prochilod sperm show short period of motility which decreases fast after activation.
Osmolality and composition of the activating solution on motility of fresh and frozen Prochilodus lineatus sperm were evaluated. Sperm was triggered in 11 solutions prepared with reverse osmosis (RO) water (∼0mOsmkg−1), and glucose or NaCl adjusted to 50, 100, 150, 200 and 250mOsmkg−1. Sperm motility rate and velocities (curvilinear=VCL, among others) were evaluated in fresh sperm at 10, 30 and 50s post-activation (spa), and in frozen sperm at 10 spa only. Sperm was frozen under a standardized methodology for this species. Fresh sperm motility was higher in samples triggered in RO (91%), in glucose at all osmolalities (90–92%) and in 50–150mOsmkg−1 NaCl (88–91%) than that in 200–250mOsmkg−1 NaCl (74–80%). Motility decreased (P<0.05) as a function of time after activation in samples activated in RO and in NaCl but not in glucose. Samples activated in 100–250mOsmkg−1 glucose yielded motility above 80%, at 50 spa. Curvilinear velocity was higher (P<0.05) in glucose-activated samples (322–357μms−1) compared to that activated in NaCl (192–283μms−1) and in RO (298μms−1). Frozen sperm motility and velocities were similar when triggered in RO, glucose or NaCl and were higher at 0–150 mOsm kg−1 (69–78% motility; 163–208μms−1 VCL) than at 200–250mOsmkg−1 (34–59% motility; 127–168μms−1 VCL). High sperm motility with fast velocity for a long period is achieved at 100–150mOsmkg−1, in glucose solution for fresh sperm and in glucose or NaCl for frozen sperm.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27601320</pmid><doi>10.1016/j.anireprosci.2016.08.014</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-2380-9497</orcidid><orcidid>https://orcid.org/0000-0002-5071-4971</orcidid></addata></record> |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals CASA Characiformes - physiology Freezing Hydrogen-Ion Concentration Male Neotropical Osmolar Concentration Osmotic pressure Semen Semen Preservation - veterinary Sodium Chloride - chemistry Sodium Chloride - pharmacology Sperm Motility - drug effects Sperm Motility - physiology Spermatozoa - physiology |
| title | Osmolality and composition of the activating solution affects motility of fresh and frozen Prochilodus lineatus sperm differently |
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