Overexpression of CXCR7 induces angiogenic capacity of human hepatocellular carcinoma cells via the AKT signaling pathway
Angiogenesis is essential for tumor growth, especially in hepatocellular carcinoma (HCC). The hypervascularity is associated with poor prognosis and highly invasive HCC. The C-X-C chemokine receptor type 7 (CXCR7) has been implied overexpressed in many tumor types. Our study aimed to investigate the...
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| Veröffentlicht in: | Oncology reports 2016-10, Vol.36 (4), p.2275-2281 |
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| description | Angiogenesis is essential for tumor growth, especially in hepatocellular carcinoma (HCC). The hypervascularity is associated with poor prognosis and highly invasive HCC. The C-X-C chemokine receptor type 7 (CXCR7) has been implied overexpressed in many tumor types. Our study aimed to investigate the CXCR7 function in HCC. The tube formation, Transwell migration assay of human umbilical vein endothelial cells (HUVECs) and chicken chorioallantoic membrane (CAM) assay were used. We confirmed that CXCR7 induces angiogenic capacity. Moreover, overexpressing CXCR7 increased the phosphorylated (but not total) AKT expression in HCC cells. Furthermore, overexpressing CXCR7 increased the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 in HCC cells. Additionally, inhibition of AKT by LY294002 abrogated CXCR7-induced angiogenic capacity in HCC cells. Our study suggested that CXCR7 plays an important pro-angiogenic role in HCC via activation of the AKT pathway. So CXCR7 may be a potential target for anti-angiogenic therapy in HCC. |
| doi_str_mv | 10.3892/or.2016.5045 |
| format | Article |
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The hypervascularity is associated with poor prognosis and highly invasive HCC. The C-X-C chemokine receptor type 7 (CXCR7) has been implied overexpressed in many tumor types. Our study aimed to investigate the CXCR7 function in HCC. The tube formation, Transwell migration assay of human umbilical vein endothelial cells (HUVECs) and chicken chorioallantoic membrane (CAM) assay were used. We confirmed that CXCR7 induces angiogenic capacity. Moreover, overexpressing CXCR7 increased the phosphorylated (but not total) AKT expression in HCC cells. Furthermore, overexpressing CXCR7 increased the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 in HCC cells. Additionally, inhibition of AKT by LY294002 abrogated CXCR7-induced angiogenic capacity in HCC cells. Our study suggested that CXCR7 plays an important pro-angiogenic role in HCC via activation of the AKT pathway. So CXCR7 may be a potential target for anti-angiogenic therapy in HCC.</description><identifier>ISSN: 1021-335X</identifier><identifier>EISSN: 1791-2431</identifier><identifier>DOI: 10.3892/or.2016.5045</identifier><identifier>PMID: 27572688</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Angiogenesis ; C-X-C chemokine receptor type 7 ; Carcinoma, Hepatocellular - drug therapy ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - pathology ; Care and treatment ; Cell adhesion & migration ; Cell growth ; Cell Line, Tumor ; Cell receptors ; Development and progression ; Embryos ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Health aspects ; Hepatitis ; hepatocellular carcinoma ; Hepatoma ; Human Umbilical Vein Endothelial Cells ; Humans ; Interleukin-6 - biosynthesis ; Interleukin-6 - genetics ; Interleukin-8 - biosynthesis ; Interleukin-8 - genetics ; Liver cancer ; Liver Neoplasms - genetics ; Liver Neoplasms - pathology ; Metastasis ; Molecular Targeted Therapy ; Neovascularization ; Neovascularization, Pathologic - drug therapy ; Neovascularization, Pathologic - genetics ; Neovascularization, Pathologic - pathology ; Physiology ; Proto-Oncogene Proteins c-akt - biosynthesis ; Proto-Oncogene Proteins c-akt - genetics ; Receptors, CXCR - biosynthesis ; Receptors, CXCR - genetics ; Signal Transduction - genetics ; Tumors ; Vascular endothelial growth factor</subject><ispartof>Oncology reports, 2016-10, Vol.36 (4), p.2275-2281</ispartof><rights>Copyright © 2016, Spandidos Publications</rights><rights>COPYRIGHT 2016 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-fd50e7479e242c39e03181fde010261c14c87afa2ba8dcfca8fe2248a97f6ef03</citedby><cites>FETCH-LOGICAL-c486t-fd50e7479e242c39e03181fde010261c14c87afa2ba8dcfca8fe2248a97f6ef03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27572688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Yuhui</creatorcontrib><creatorcontrib>Teng, Fei</creatorcontrib><creatorcontrib>Wang, Geying</creatorcontrib><creatorcontrib>Nie, Zhiyu</creatorcontrib><title>Overexpression of CXCR7 induces angiogenic capacity of human hepatocellular carcinoma cells via the AKT signaling pathway</title><title>Oncology reports</title><addtitle>Oncol Rep</addtitle><description>Angiogenesis is essential for tumor growth, especially in hepatocellular carcinoma (HCC). The hypervascularity is associated with poor prognosis and highly invasive HCC. The C-X-C chemokine receptor type 7 (CXCR7) has been implied overexpressed in many tumor types. Our study aimed to investigate the CXCR7 function in HCC. The tube formation, Transwell migration assay of human umbilical vein endothelial cells (HUVECs) and chicken chorioallantoic membrane (CAM) assay were used. We confirmed that CXCR7 induces angiogenic capacity. Moreover, overexpressing CXCR7 increased the phosphorylated (but not total) AKT expression in HCC cells. Furthermore, overexpressing CXCR7 increased the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 in HCC cells. Additionally, inhibition of AKT by LY294002 abrogated CXCR7-induced angiogenic capacity in HCC cells. Our study suggested that CXCR7 plays an important pro-angiogenic role in HCC via activation of the AKT pathway. So CXCR7 may be a potential target for anti-angiogenic therapy in HCC.</description><subject>Angiogenesis</subject><subject>C-X-C chemokine receptor type 7</subject><subject>Carcinoma, Hepatocellular - drug therapy</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Care and treatment</subject><subject>Cell adhesion & migration</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell receptors</subject><subject>Development and progression</subject><subject>Embryos</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Hepatitis</subject><subject>hepatocellular carcinoma</subject><subject>Hepatoma</subject><subject>Human Umbilical Vein Endothelial Cells</subject><subject>Humans</subject><subject>Interleukin-6 - biosynthesis</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukin-8 - biosynthesis</subject><subject>Interleukin-8 - genetics</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - pathology</subject><subject>Metastasis</subject><subject>Molecular Targeted Therapy</subject><subject>Neovascularization</subject><subject>Neovascularization, Pathologic - drug therapy</subject><subject>Neovascularization, Pathologic - genetics</subject><subject>Neovascularization, Pathologic - pathology</subject><subject>Physiology</subject><subject>Proto-Oncogene Proteins c-akt - biosynthesis</subject><subject>Proto-Oncogene Proteins c-akt - genetics</subject><subject>Receptors, CXCR - biosynthesis</subject><subject>Receptors, CXCR - genetics</subject><subject>Signal Transduction - genetics</subject><subject>Tumors</subject><subject>Vascular endothelial growth factor</subject><issn>1021-335X</issn><issn>1791-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpt0t1r1TAUAPAiipvTN58lIIgP9pqPNkkfLxe_cDCQCXsrZ-nJbUab1KSd3v_elM3NieQh4fA7yTnkFMVLRjdCN_x9iBtOmdzUtKofFcdMNazklWCP85lyVgpRXxwVz1K6opQrKpunxRFXteJS6-PicHaNEX9NEVNywZNgye5i900R57vFYCLg9y7s0TtDDExg3HxYUb-M4EmPE8zB4DAsA8QMonE-jEDWUCLXDsjcI9l-PSfJ7T0Mzu9JTul_wuF58cTCkPDF7X5SfP_44Xz3uTw9-_Rltz0tTaXlXNqupqgq1SCvuBENUsE0sx3S3J1khlVGK7DAL0F3xhrQFjmvNDTKSrRUnBRvb-6dYvixYJrb0aW1PvAYltQyzWndCCV0pq__oVdhibnsrBrBpeK6YfdqDwO2ztswRzDrpe22klJXspZ1Vpv_qLw6HJ0JHq3L8QcJb_5K6BGGuU9hWOb8LekhfHcDTQwpRbTtFN0I8dAy2q4j0YbYriPRriOR-avbppbLEbs7_GcG7h9OE_jOdSHdmRBLIUtalTxr8RtwOLzc</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Chen, Yuhui</creator><creator>Teng, Fei</creator><creator>Wang, Geying</creator><creator>Nie, Zhiyu</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20161001</creationdate><title>Overexpression of CXCR7 induces angiogenic capacity of human hepatocellular carcinoma cells via the AKT signaling pathway</title><author>Chen, Yuhui ; Teng, Fei ; Wang, Geying ; Nie, Zhiyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-fd50e7479e242c39e03181fde010261c14c87afa2ba8dcfca8fe2248a97f6ef03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Angiogenesis</topic><topic>C-X-C chemokine receptor type 7</topic><topic>Carcinoma, Hepatocellular - drug therapy</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Care and treatment</topic><topic>Cell adhesion & migration</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell receptors</topic><topic>Development and progression</topic><topic>Embryos</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Hepatitis</topic><topic>hepatocellular carcinoma</topic><topic>Hepatoma</topic><topic>Human Umbilical Vein Endothelial Cells</topic><topic>Humans</topic><topic>Interleukin-6 - biosynthesis</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukin-8 - biosynthesis</topic><topic>Interleukin-8 - genetics</topic><topic>Liver cancer</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - pathology</topic><topic>Metastasis</topic><topic>Molecular Targeted Therapy</topic><topic>Neovascularization</topic><topic>Neovascularization, Pathologic - drug therapy</topic><topic>Neovascularization, Pathologic - genetics</topic><topic>Neovascularization, Pathologic - pathology</topic><topic>Physiology</topic><topic>Proto-Oncogene Proteins c-akt - biosynthesis</topic><topic>Proto-Oncogene Proteins c-akt - genetics</topic><topic>Receptors, CXCR - biosynthesis</topic><topic>Receptors, CXCR - genetics</topic><topic>Signal Transduction - genetics</topic><topic>Tumors</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Yuhui</creatorcontrib><creatorcontrib>Teng, Fei</creatorcontrib><creatorcontrib>Wang, Geying</creatorcontrib><creatorcontrib>Nie, Zhiyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Oncology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Yuhui</au><au>Teng, Fei</au><au>Wang, Geying</au><au>Nie, Zhiyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of CXCR7 induces angiogenic capacity of human hepatocellular carcinoma cells via the AKT signaling pathway</atitle><jtitle>Oncology reports</jtitle><addtitle>Oncol Rep</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>36</volume><issue>4</issue><spage>2275</spage><epage>2281</epage><pages>2275-2281</pages><issn>1021-335X</issn><eissn>1791-2431</eissn><abstract>Angiogenesis is essential for tumor growth, especially in hepatocellular carcinoma (HCC). The hypervascularity is associated with poor prognosis and highly invasive HCC. The C-X-C chemokine receptor type 7 (CXCR7) has been implied overexpressed in many tumor types. Our study aimed to investigate the CXCR7 function in HCC. The tube formation, Transwell migration assay of human umbilical vein endothelial cells (HUVECs) and chicken chorioallantoic membrane (CAM) assay were used. We confirmed that CXCR7 induces angiogenic capacity. Moreover, overexpressing CXCR7 increased the phosphorylated (but not total) AKT expression in HCC cells. Furthermore, overexpressing CXCR7 increased the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 in HCC cells. Additionally, inhibition of AKT by LY294002 abrogated CXCR7-induced angiogenic capacity in HCC cells. Our study suggested that CXCR7 plays an important pro-angiogenic role in HCC via activation of the AKT pathway. So CXCR7 may be a potential target for anti-angiogenic therapy in HCC.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>27572688</pmid><doi>10.3892/or.2016.5045</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Oncology reports, 2016-10, Vol.36 (4), p.2275-2281 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Angiogenesis C-X-C chemokine receptor type 7 Carcinoma, Hepatocellular - drug therapy Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - pathology Care and treatment Cell adhesion & migration Cell growth Cell Line, Tumor Cell receptors Development and progression Embryos Gene expression Gene Expression Regulation, Neoplastic Genetic aspects Health aspects Hepatitis hepatocellular carcinoma Hepatoma Human Umbilical Vein Endothelial Cells Humans Interleukin-6 - biosynthesis Interleukin-6 - genetics Interleukin-8 - biosynthesis Interleukin-8 - genetics Liver cancer Liver Neoplasms - genetics Liver Neoplasms - pathology Metastasis Molecular Targeted Therapy Neovascularization Neovascularization, Pathologic - drug therapy Neovascularization, Pathologic - genetics Neovascularization, Pathologic - pathology Physiology Proto-Oncogene Proteins c-akt - biosynthesis Proto-Oncogene Proteins c-akt - genetics Receptors, CXCR - biosynthesis Receptors, CXCR - genetics Signal Transduction - genetics Tumors Vascular endothelial growth factor |
| title | Overexpression of CXCR7 induces angiogenic capacity of human hepatocellular carcinoma cells via the AKT signaling pathway |
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