Quantitative bioimaging of platinum group elements in tumor spheroids
Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elem...
Gespeichert in:
| Veröffentlicht in: | Analytica chimica acta 2016-09, Vol.938, p.106-113 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 113 |
|---|---|
| container_issue | |
| container_start_page | 106 |
| container_title | Analytica chimica acta |
| container_volume | 938 |
| creator | Niehoff, Ann-Christin Grünebaum, Jonas Moosmann, Aline Mulac, Dennis Söbbing, Judith Niehaus, Rebecca Buchholz, Rebecca Kröger, Sabrina Wiehe, Arno Wagner, Sylvia Sperling, Michael von Briesen, Hagen Langer, Klaus Karst, Uwe |
| description | Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method.
[Display omitted]
•A method for quantitative elemental imaging analysis in tumor spheroids was developed.•Laser ablation-inductively coupled plasma-mass spectrometry imaging was used.•External quantification was carried out by elemental standards embedded in gelatin.•Enrichment of platinum and palladium complexes in the spheroids was shown. |
| doi_str_mv | 10.1016/j.aca.2016.07.021 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1819432265</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S000326701630842X</els_id><sourcerecordid>1819432265</sourcerecordid><originalsourceid>FETCH-LOGICAL-c353t-99a14bdb172cfa81138659f2a0b76ef9b1a3f950a3dcd928fc6a9358f8a21b2a3</originalsourceid><addsrcrecordid>eNp9kEtLxDAUhYMozjj6A9xIl25ac5PpI7iSYXyAIIKuQ5rejBnapibtgP_eyIwuXd0H5xw4HyGXQDOgUNxsM6VVxuKa0TKjDI7IHKqSp0vOlsdkTinlKStKOiNnIWzjyYAuT8mMlQUIKticrF8n1Y92VKPdYVJbZzu1sf0mcSYZ2vjtpy7ZeDcNCbbYYT-GxPbJOHXOJ2H4QO9sE87JiVFtwIvDXJD3-_Xb6jF9fnl4Wt09p5rnfEyFULCsmxpKpo2qAHhV5MIwReuyQCNqUNyInCre6EawyuhCCZ5XplIMaqb4glzvcwfvPicMo-xs0Ni2qkc3BQkViNidFXmUwl6qvQvBo5GDj938lwQqf-jJrYz05A89SUsZ0UTP1SF-qjts_hy_uKLgdi_AWHJn0cugLfYaG-tRj7Jx9p_4byuzgBw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1819432265</pqid></control><display><type>article</type><title>Quantitative bioimaging of platinum group elements in tumor spheroids</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Niehoff, Ann-Christin ; Grünebaum, Jonas ; Moosmann, Aline ; Mulac, Dennis ; Söbbing, Judith ; Niehaus, Rebecca ; Buchholz, Rebecca ; Kröger, Sabrina ; Wiehe, Arno ; Wagner, Sylvia ; Sperling, Michael ; von Briesen, Hagen ; Langer, Klaus ; Karst, Uwe</creator><creatorcontrib>Niehoff, Ann-Christin ; Grünebaum, Jonas ; Moosmann, Aline ; Mulac, Dennis ; Söbbing, Judith ; Niehaus, Rebecca ; Buchholz, Rebecca ; Kröger, Sabrina ; Wiehe, Arno ; Wagner, Sylvia ; Sperling, Michael ; von Briesen, Hagen ; Langer, Klaus ; Karst, Uwe</creatorcontrib><description>Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method.
[Display omitted]
•A method for quantitative elemental imaging analysis in tumor spheroids was developed.•Laser ablation-inductively coupled plasma-mass spectrometry imaging was used.•External quantification was carried out by elemental standards embedded in gelatin.•Enrichment of platinum and palladium complexes in the spheroids was shown.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2016.07.021</identifier><identifier>PMID: 27619092</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Cell Line, Tumor ; Humans ; LA-ICP-MS ; Mass Spectrometry - methods ; Neoplasms - chemistry ; Neoplasms - metabolism ; Palladium - chemistry ; Palladium - pharmacokinetics ; Photosensitizer ; Platinum Compounds - analysis ; Pt cytostatics ; Quantification ; Tumor spheroids</subject><ispartof>Analytica chimica acta, 2016-09, Vol.938, p.106-113</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-99a14bdb172cfa81138659f2a0b76ef9b1a3f950a3dcd928fc6a9358f8a21b2a3</citedby><cites>FETCH-LOGICAL-c353t-99a14bdb172cfa81138659f2a0b76ef9b1a3f950a3dcd928fc6a9358f8a21b2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aca.2016.07.021$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27619092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Niehoff, Ann-Christin</creatorcontrib><creatorcontrib>Grünebaum, Jonas</creatorcontrib><creatorcontrib>Moosmann, Aline</creatorcontrib><creatorcontrib>Mulac, Dennis</creatorcontrib><creatorcontrib>Söbbing, Judith</creatorcontrib><creatorcontrib>Niehaus, Rebecca</creatorcontrib><creatorcontrib>Buchholz, Rebecca</creatorcontrib><creatorcontrib>Kröger, Sabrina</creatorcontrib><creatorcontrib>Wiehe, Arno</creatorcontrib><creatorcontrib>Wagner, Sylvia</creatorcontrib><creatorcontrib>Sperling, Michael</creatorcontrib><creatorcontrib>von Briesen, Hagen</creatorcontrib><creatorcontrib>Langer, Klaus</creatorcontrib><creatorcontrib>Karst, Uwe</creatorcontrib><title>Quantitative bioimaging of platinum group elements in tumor spheroids</title><title>Analytica chimica acta</title><addtitle>Anal Chim Acta</addtitle><description>Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method.
[Display omitted]
•A method for quantitative elemental imaging analysis in tumor spheroids was developed.•Laser ablation-inductively coupled plasma-mass spectrometry imaging was used.•External quantification was carried out by elemental standards embedded in gelatin.•Enrichment of platinum and palladium complexes in the spheroids was shown.</description><subject>Cell Line, Tumor</subject><subject>Humans</subject><subject>LA-ICP-MS</subject><subject>Mass Spectrometry - methods</subject><subject>Neoplasms - chemistry</subject><subject>Neoplasms - metabolism</subject><subject>Palladium - chemistry</subject><subject>Palladium - pharmacokinetics</subject><subject>Photosensitizer</subject><subject>Platinum Compounds - analysis</subject><subject>Pt cytostatics</subject><subject>Quantification</subject><subject>Tumor spheroids</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAUhYMozjj6A9xIl25ac5PpI7iSYXyAIIKuQ5rejBnapibtgP_eyIwuXd0H5xw4HyGXQDOgUNxsM6VVxuKa0TKjDI7IHKqSp0vOlsdkTinlKStKOiNnIWzjyYAuT8mMlQUIKticrF8n1Y92VKPdYVJbZzu1sf0mcSYZ2vjtpy7ZeDcNCbbYYT-GxPbJOHXOJ2H4QO9sE87JiVFtwIvDXJD3-_Xb6jF9fnl4Wt09p5rnfEyFULCsmxpKpo2qAHhV5MIwReuyQCNqUNyInCre6EawyuhCCZ5XplIMaqb4glzvcwfvPicMo-xs0Ni2qkc3BQkViNidFXmUwl6qvQvBo5GDj938lwQqf-jJrYz05A89SUsZ0UTP1SF-qjts_hy_uKLgdi_AWHJn0cugLfYaG-tRj7Jx9p_4byuzgBw</recordid><startdate>20160928</startdate><enddate>20160928</enddate><creator>Niehoff, Ann-Christin</creator><creator>Grünebaum, Jonas</creator><creator>Moosmann, Aline</creator><creator>Mulac, Dennis</creator><creator>Söbbing, Judith</creator><creator>Niehaus, Rebecca</creator><creator>Buchholz, Rebecca</creator><creator>Kröger, Sabrina</creator><creator>Wiehe, Arno</creator><creator>Wagner, Sylvia</creator><creator>Sperling, Michael</creator><creator>von Briesen, Hagen</creator><creator>Langer, Klaus</creator><creator>Karst, Uwe</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160928</creationdate><title>Quantitative bioimaging of platinum group elements in tumor spheroids</title><author>Niehoff, Ann-Christin ; Grünebaum, Jonas ; Moosmann, Aline ; Mulac, Dennis ; Söbbing, Judith ; Niehaus, Rebecca ; Buchholz, Rebecca ; Kröger, Sabrina ; Wiehe, Arno ; Wagner, Sylvia ; Sperling, Michael ; von Briesen, Hagen ; Langer, Klaus ; Karst, Uwe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-99a14bdb172cfa81138659f2a0b76ef9b1a3f950a3dcd928fc6a9358f8a21b2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Cell Line, Tumor</topic><topic>Humans</topic><topic>LA-ICP-MS</topic><topic>Mass Spectrometry - methods</topic><topic>Neoplasms - chemistry</topic><topic>Neoplasms - metabolism</topic><topic>Palladium - chemistry</topic><topic>Palladium - pharmacokinetics</topic><topic>Photosensitizer</topic><topic>Platinum Compounds - analysis</topic><topic>Pt cytostatics</topic><topic>Quantification</topic><topic>Tumor spheroids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Niehoff, Ann-Christin</creatorcontrib><creatorcontrib>Grünebaum, Jonas</creatorcontrib><creatorcontrib>Moosmann, Aline</creatorcontrib><creatorcontrib>Mulac, Dennis</creatorcontrib><creatorcontrib>Söbbing, Judith</creatorcontrib><creatorcontrib>Niehaus, Rebecca</creatorcontrib><creatorcontrib>Buchholz, Rebecca</creatorcontrib><creatorcontrib>Kröger, Sabrina</creatorcontrib><creatorcontrib>Wiehe, Arno</creatorcontrib><creatorcontrib>Wagner, Sylvia</creatorcontrib><creatorcontrib>Sperling, Michael</creatorcontrib><creatorcontrib>von Briesen, Hagen</creatorcontrib><creatorcontrib>Langer, Klaus</creatorcontrib><creatorcontrib>Karst, Uwe</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Niehoff, Ann-Christin</au><au>Grünebaum, Jonas</au><au>Moosmann, Aline</au><au>Mulac, Dennis</au><au>Söbbing, Judith</au><au>Niehaus, Rebecca</au><au>Buchholz, Rebecca</au><au>Kröger, Sabrina</au><au>Wiehe, Arno</au><au>Wagner, Sylvia</au><au>Sperling, Michael</au><au>von Briesen, Hagen</au><au>Langer, Klaus</au><au>Karst, Uwe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative bioimaging of platinum group elements in tumor spheroids</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2016-09-28</date><risdate>2016</risdate><volume>938</volume><spage>106</spage><epage>113</epage><pages>106-113</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><abstract>Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method.
[Display omitted]
•A method for quantitative elemental imaging analysis in tumor spheroids was developed.•Laser ablation-inductively coupled plasma-mass spectrometry imaging was used.•External quantification was carried out by elemental standards embedded in gelatin.•Enrichment of platinum and palladium complexes in the spheroids was shown.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27619092</pmid><doi>10.1016/j.aca.2016.07.021</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2670 |
| ispartof | Analytica chimica acta, 2016-09, Vol.938, p.106-113 |
| issn | 0003-2670 1873-4324 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1819432265 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Cell Line, Tumor Humans LA-ICP-MS Mass Spectrometry - methods Neoplasms - chemistry Neoplasms - metabolism Palladium - chemistry Palladium - pharmacokinetics Photosensitizer Platinum Compounds - analysis Pt cytostatics Quantification Tumor spheroids |
| title | Quantitative bioimaging of platinum group elements in tumor spheroids |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T10%3A13%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitative%20bioimaging%20of%20platinum%20group%20elements%20in%20tumor%20spheroids&rft.jtitle=Analytica%20chimica%20acta&rft.au=Niehoff,%20Ann-Christin&rft.date=2016-09-28&rft.volume=938&rft.spage=106&rft.epage=113&rft.pages=106-113&rft.issn=0003-2670&rft.eissn=1873-4324&rft_id=info:doi/10.1016/j.aca.2016.07.021&rft_dat=%3Cproquest_cross%3E1819432265%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1819432265&rft_id=info:pmid/27619092&rft_els_id=S000326701630842X&rfr_iscdi=true |