A Relation Between Cell Cycle and Intestinal Metaplasia in Oesophageal Biopsies Using Optical and Digital Microscopy
Protein expression changes in relation to cell cycles provide important information, and it may represent a new method for an early diagnosis of metaplasia – dysplasia – adenocarcinoma sequence. We investigated potential changes in cell cycle genes such as protooncogenes (PCNA, EGFR), tumour suppres...
Gespeichert in:
| Veröffentlicht in: | Pathology oncology research 2015-07, Vol.21 (3), p.669-673 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 673 |
|---|---|
| container_issue | 3 |
| container_start_page | 669 |
| container_title | Pathology oncology research |
| container_volume | 21 |
| creator | Mate, Miklos Molnar, Bela |
| description | Protein expression changes in relation to cell cycles provide important information, and it may represent a new method for an early diagnosis of metaplasia – dysplasia – adenocarcinoma sequence. We investigated potential changes in cell cycle genes such as protooncogenes (PCNA, EGFR), tumour suppressor gene (p53), apoptotic TUNNEL (Tdt mediated dUTP nick and labelling) gene, as well as small intestinal mucus antigen (SIMA) and large intestinal mucus antigen (LIMA), which accumulates in metaplastic epithelium due to the inflammatory process in routine oesophageal biopsies using immunohistochemistry. Oesophageal biopsies were taken from patients with Barrett’s oesophagus (
n
= 30), reflux oesophagitis (
n
= 30), healthy oesophagus (
n
= 30) and healthy cardia (
n
= 10). Immunohistochemical signalling was carried out by Streptavidin-Biotin-AEC (aminoetil-carbazol). Expression of PCNA was statistically significantly lower in healthy oesophagus (
p
0.05). |
| doi_str_mv | 10.1007/s12253-014-9873-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1819147250</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1819147250</sourcerecordid><originalsourceid>FETCH-LOGICAL-c427t-d9ef50f36fb0476efde3d1ee51f8ac01f38985db4ffa35eeff34cd39b35a66cc3</originalsourceid><addsrcrecordid>eNqFkV1LHDEYhYNUqlV_QG8k0JveTM33JJe6_RIsC0WvQzbzZhuZzYyTLGX_fTNdLVIQr_LxPueEk4PQe0o-UULai0wZk7whVDRGt7zRB-iYSs4apkn7pu4ZNY0wUh2hdznfk6pRRr1FR0y2oh7oMSqX-Cf0rsQh4SsovwESXkDf48XO94Bd6vB1KpBLTK7HP6C4sXc5OhwTXkIexl9uDXVyFYcxR8j4Lse0xsuxRF-vZ_3nuI5lFkc_DdkP4-4UHQbXZzh7XE_Q3dcvt4vvzc3y2_Xi8qbxgrWl6QwESQJXYUVEqyB0wDsKIGnQzhMauDZadisRguMSIAQufMfNikunlPf8BH3c-47T8LCtIewmZl_TuQTDNluqqaGiZZK8jiqtiNLCiIp--A-9H7ZT_Z6_lKRCSC0rRffUHDpPEOw4xY2bdpYSO7dn9-3Z2p6d27O6as4fnberDXT_FE91VYDtgVxHaQ3Ts6dfdP0DFVilfQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1685144585</pqid></control><display><type>article</type><title>A Relation Between Cell Cycle and Intestinal Metaplasia in Oesophageal Biopsies Using Optical and Digital Microscopy</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Mate, Miklos ; Molnar, Bela</creator><creatorcontrib>Mate, Miklos ; Molnar, Bela</creatorcontrib><description>Protein expression changes in relation to cell cycles provide important information, and it may represent a new method for an early diagnosis of metaplasia – dysplasia – adenocarcinoma sequence. We investigated potential changes in cell cycle genes such as protooncogenes (PCNA, EGFR), tumour suppressor gene (p53), apoptotic TUNNEL (Tdt mediated dUTP nick and labelling) gene, as well as small intestinal mucus antigen (SIMA) and large intestinal mucus antigen (LIMA), which accumulates in metaplastic epithelium due to the inflammatory process in routine oesophageal biopsies using immunohistochemistry. Oesophageal biopsies were taken from patients with Barrett’s oesophagus (
n
= 30), reflux oesophagitis (
n
= 30), healthy oesophagus (
n
= 30) and healthy cardia (
n
= 10). Immunohistochemical signalling was carried out by Streptavidin-Biotin-AEC (aminoetil-carbazol). Expression of PCNA was statistically significantly lower in healthy oesophagus (
p
< 0.05) versus reflux oesophagitis and Barrett’s oesophagus. However, no significant change was detected in the expression of SIMA and LIMA in intestinal metaplasia. Further, EGFR, p53 and TUNNEL levels were significantly different in healthy versus Barrett’s oesophagus. Manual counting using virtual microscopy was comparable with the result using conventional light microscopy, but the former is significantly quicker. There was no difference between manual and automated cell counting (
p
> 0.05).</description><identifier>ISSN: 1219-4956</identifier><identifier>EISSN: 1532-2807</identifier><identifier>DOI: 10.1007/s12253-014-9873-8</identifier><identifier>PMID: 25740071</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Barrett Esophagus - metabolism ; Barrett Esophagus - pathology ; Barrett Esophagus - surgery ; Biomarkers - metabolism ; Biomedical and Life Sciences ; Biomedicine ; Biopsy ; Cancer Research ; Cell Cycle ; Esophagus - metabolism ; Esophagus - pathology ; Esophagus - surgery ; Humans ; Image Interpretation, Computer-Assisted ; Immunoenzyme Techniques ; Immunology ; Intestinal Diseases - metabolism ; Intestinal Diseases - pathology ; Intestinal Diseases - surgery ; Metaplasia - metabolism ; Metaplasia - pathology ; Metaplasia - surgery ; Microscopy - instrumentation ; Oncology ; Optical Imaging ; Pathology ; Prognosis</subject><ispartof>Pathology oncology research, 2015-07, Vol.21 (3), p.669-673</ispartof><rights>Arányi Lajos Foundation 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c427t-d9ef50f36fb0476efde3d1ee51f8ac01f38985db4ffa35eeff34cd39b35a66cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12253-014-9873-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12253-014-9873-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27915,27916,41479,42548,51310</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25740071$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mate, Miklos</creatorcontrib><creatorcontrib>Molnar, Bela</creatorcontrib><title>A Relation Between Cell Cycle and Intestinal Metaplasia in Oesophageal Biopsies Using Optical and Digital Microscopy</title><title>Pathology oncology research</title><addtitle>Pathol. Oncol. Res</addtitle><addtitle>Pathol Oncol Res</addtitle><description>Protein expression changes in relation to cell cycles provide important information, and it may represent a new method for an early diagnosis of metaplasia – dysplasia – adenocarcinoma sequence. We investigated potential changes in cell cycle genes such as protooncogenes (PCNA, EGFR), tumour suppressor gene (p53), apoptotic TUNNEL (Tdt mediated dUTP nick and labelling) gene, as well as small intestinal mucus antigen (SIMA) and large intestinal mucus antigen (LIMA), which accumulates in metaplastic epithelium due to the inflammatory process in routine oesophageal biopsies using immunohistochemistry. Oesophageal biopsies were taken from patients with Barrett’s oesophagus (
n
= 30), reflux oesophagitis (
n
= 30), healthy oesophagus (
n
= 30) and healthy cardia (
n
= 10). Immunohistochemical signalling was carried out by Streptavidin-Biotin-AEC (aminoetil-carbazol). Expression of PCNA was statistically significantly lower in healthy oesophagus (
p
< 0.05) versus reflux oesophagitis and Barrett’s oesophagus. However, no significant change was detected in the expression of SIMA and LIMA in intestinal metaplasia. Further, EGFR, p53 and TUNNEL levels were significantly different in healthy versus Barrett’s oesophagus. Manual counting using virtual microscopy was comparable with the result using conventional light microscopy, but the former is significantly quicker. There was no difference between manual and automated cell counting (
p
> 0.05).</description><subject>Barrett Esophagus - metabolism</subject><subject>Barrett Esophagus - pathology</subject><subject>Barrett Esophagus - surgery</subject><subject>Biomarkers - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biopsy</subject><subject>Cancer Research</subject><subject>Cell Cycle</subject><subject>Esophagus - metabolism</subject><subject>Esophagus - pathology</subject><subject>Esophagus - surgery</subject><subject>Humans</subject><subject>Image Interpretation, Computer-Assisted</subject><subject>Immunoenzyme Techniques</subject><subject>Immunology</subject><subject>Intestinal Diseases - metabolism</subject><subject>Intestinal Diseases - pathology</subject><subject>Intestinal Diseases - surgery</subject><subject>Metaplasia - metabolism</subject><subject>Metaplasia - pathology</subject><subject>Metaplasia - surgery</subject><subject>Microscopy - instrumentation</subject><subject>Oncology</subject><subject>Optical Imaging</subject><subject>Pathology</subject><subject>Prognosis</subject><issn>1219-4956</issn><issn>1532-2807</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkV1LHDEYhYNUqlV_QG8k0JveTM33JJe6_RIsC0WvQzbzZhuZzYyTLGX_fTNdLVIQr_LxPueEk4PQe0o-UULai0wZk7whVDRGt7zRB-iYSs4apkn7pu4ZNY0wUh2hdznfk6pRRr1FR0y2oh7oMSqX-Cf0rsQh4SsovwESXkDf48XO94Bd6vB1KpBLTK7HP6C4sXc5OhwTXkIexl9uDXVyFYcxR8j4Lse0xsuxRF-vZ_3nuI5lFkc_DdkP4-4UHQbXZzh7XE_Q3dcvt4vvzc3y2_Xi8qbxgrWl6QwESQJXYUVEqyB0wDsKIGnQzhMauDZadisRguMSIAQufMfNikunlPf8BH3c-47T8LCtIewmZl_TuQTDNluqqaGiZZK8jiqtiNLCiIp--A-9H7ZT_Z6_lKRCSC0rRffUHDpPEOw4xY2bdpYSO7dn9-3Z2p6d27O6as4fnberDXT_FE91VYDtgVxHaQ3Ts6dfdP0DFVilfQ</recordid><startdate>20150701</startdate><enddate>20150701</enddate><creator>Mate, Miklos</creator><creator>Molnar, Bela</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20150701</creationdate><title>A Relation Between Cell Cycle and Intestinal Metaplasia in Oesophageal Biopsies Using Optical and Digital Microscopy</title><author>Mate, Miklos ; Molnar, Bela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-d9ef50f36fb0476efde3d1ee51f8ac01f38985db4ffa35eeff34cd39b35a66cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Barrett Esophagus - metabolism</topic><topic>Barrett Esophagus - pathology</topic><topic>Barrett Esophagus - surgery</topic><topic>Biomarkers - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biopsy</topic><topic>Cancer Research</topic><topic>Cell Cycle</topic><topic>Esophagus - metabolism</topic><topic>Esophagus - pathology</topic><topic>Esophagus - surgery</topic><topic>Humans</topic><topic>Image Interpretation, Computer-Assisted</topic><topic>Immunoenzyme Techniques</topic><topic>Immunology</topic><topic>Intestinal Diseases - metabolism</topic><topic>Intestinal Diseases - pathology</topic><topic>Intestinal Diseases - surgery</topic><topic>Metaplasia - metabolism</topic><topic>Metaplasia - pathology</topic><topic>Metaplasia - surgery</topic><topic>Microscopy - instrumentation</topic><topic>Oncology</topic><topic>Optical Imaging</topic><topic>Pathology</topic><topic>Prognosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mate, Miklos</creatorcontrib><creatorcontrib>Molnar, Bela</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Pathology oncology research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mate, Miklos</au><au>Molnar, Bela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Relation Between Cell Cycle and Intestinal Metaplasia in Oesophageal Biopsies Using Optical and Digital Microscopy</atitle><jtitle>Pathology oncology research</jtitle><stitle>Pathol. Oncol. Res</stitle><addtitle>Pathol Oncol Res</addtitle><date>2015-07-01</date><risdate>2015</risdate><volume>21</volume><issue>3</issue><spage>669</spage><epage>673</epage><pages>669-673</pages><issn>1219-4956</issn><eissn>1532-2807</eissn><abstract>Protein expression changes in relation to cell cycles provide important information, and it may represent a new method for an early diagnosis of metaplasia – dysplasia – adenocarcinoma sequence. We investigated potential changes in cell cycle genes such as protooncogenes (PCNA, EGFR), tumour suppressor gene (p53), apoptotic TUNNEL (Tdt mediated dUTP nick and labelling) gene, as well as small intestinal mucus antigen (SIMA) and large intestinal mucus antigen (LIMA), which accumulates in metaplastic epithelium due to the inflammatory process in routine oesophageal biopsies using immunohistochemistry. Oesophageal biopsies were taken from patients with Barrett’s oesophagus (
n
= 30), reflux oesophagitis (
n
= 30), healthy oesophagus (
n
= 30) and healthy cardia (
n
= 10). Immunohistochemical signalling was carried out by Streptavidin-Biotin-AEC (aminoetil-carbazol). Expression of PCNA was statistically significantly lower in healthy oesophagus (
p
< 0.05) versus reflux oesophagitis and Barrett’s oesophagus. However, no significant change was detected in the expression of SIMA and LIMA in intestinal metaplasia. Further, EGFR, p53 and TUNNEL levels were significantly different in healthy versus Barrett’s oesophagus. Manual counting using virtual microscopy was comparable with the result using conventional light microscopy, but the former is significantly quicker. There was no difference between manual and automated cell counting (
p
> 0.05).</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>25740071</pmid><doi>10.1007/s12253-014-9873-8</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1219-4956 |
| ispartof | Pathology oncology research, 2015-07, Vol.21 (3), p.669-673 |
| issn | 1219-4956 1532-2807 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1819147250 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Barrett Esophagus - metabolism Barrett Esophagus - pathology Barrett Esophagus - surgery Biomarkers - metabolism Biomedical and Life Sciences Biomedicine Biopsy Cancer Research Cell Cycle Esophagus - metabolism Esophagus - pathology Esophagus - surgery Humans Image Interpretation, Computer-Assisted Immunoenzyme Techniques Immunology Intestinal Diseases - metabolism Intestinal Diseases - pathology Intestinal Diseases - surgery Metaplasia - metabolism Metaplasia - pathology Metaplasia - surgery Microscopy - instrumentation Oncology Optical Imaging Pathology Prognosis |
| title | A Relation Between Cell Cycle and Intestinal Metaplasia in Oesophageal Biopsies Using Optical and Digital Microscopy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T23%3A56%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Relation%20Between%20Cell%20Cycle%20and%20Intestinal%20Metaplasia%20in%20Oesophageal%20Biopsies%20Using%20Optical%20and%20Digital%20Microscopy&rft.jtitle=Pathology%20oncology%20research&rft.au=Mate,%20Miklos&rft.date=2015-07-01&rft.volume=21&rft.issue=3&rft.spage=669&rft.epage=673&rft.pages=669-673&rft.issn=1219-4956&rft.eissn=1532-2807&rft_id=info:doi/10.1007/s12253-014-9873-8&rft_dat=%3Cproquest_cross%3E1819147250%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1685144585&rft_id=info:pmid/25740071&rfr_iscdi=true |