Effects of 2'-O-methyl nucleotide on ligation capability of T4 DNA ligase

To further understand the ligation mechanism, effects of 2'-O-methyl nucleotide (2'-OMeN) on the T4 DNA ligation efficiency were investigated. Fluorescence resonance energy transfer assay was used to monitor the nick-joining process by T4 DNA ligase. Results showed that substitutions at 5&...

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Veröffentlicht in:	Acta biochimica et biophysica Sinica 2014-09, Vol.46 (9), p.727-737
Hauptverfasser:	Zhao, Bin, Tong, Zhaoxue, Zhao, Guojie, Mu, Runqing, Shang, Hong, Guan, Yifu
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Pair Mismatch DNA Ligases - metabolism Fluorescence Resonance Energy Transfer Kinetics Nucleotides - metabolism
Online-Zugang:	Volltext
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