Three-Dimensional Control of DNA Hybridization by Orthogonal Two-Color Two-Photon Uncaging
We successfully introduced two‐photon‐sensitive photolabile groups ([7‐(diethylamino)coumarin‐4‐yl]methyl and p‐dialkylaminonitrobiphenyl) into DNA strands and demonstrated their suitability for three‐dimensional photorelease. To visualize the uncaging, we used a fluorescence readout based on double...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2016-07, Vol.55 (31), p.8948-8952 |
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| creator | Fichte, Manuela A. H. Weyel, Xenia M. M. Junek, Stephan Schäfer, Florian Herbivo, Cyril Goeldner, Maurice Specht, Alexandre Wachtveitl, Josef Heckel, Alexander |
| description | We successfully introduced two‐photon‐sensitive photolabile groups ([7‐(diethylamino)coumarin‐4‐yl]methyl and p‐dialkylaminonitrobiphenyl) into DNA strands and demonstrated their suitability for three‐dimensional photorelease. To visualize the uncaging, we used a fluorescence readout based on double‐strand displacement in a hydrogel and in neurons. Orthogonal two‐photon uncaging of the two cages is possible, thus enabling complex scenarios of three‐dimensional control of hybridization with light.
Now also in 3D! Oligonucleotides with different sequences could be photoactivated with exact three‐dimensional control when different two‐photon‐sensitive photolabile groups were introduced into the strands. Uncaging was visualized by fluorescence as a result of double‐strand displacement. By using distinct irradiation conditions, either one of two sequences could be addressed selectively (see left‐hand images, which are overlayed on the right). |
| doi_str_mv | 10.1002/anie.201603281 |
| format | Article |
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Now also in 3D! Oligonucleotides with different sequences could be photoactivated with exact three‐dimensional control when different two‐photon‐sensitive photolabile groups were introduced into the strands. Uncaging was visualized by fluorescence as a result of double‐strand displacement. By using distinct irradiation conditions, either one of two sequences could be addressed selectively (see left‐hand images, which are overlayed on the right).</description><edition>International ed. in English</edition><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.201603281</identifier><identifier>PMID: 27294300</identifier><identifier>CODEN: ACIEAY</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Deoxyribonucleic acid ; DNA ; hydrogels ; photochemistry ; two-photon uncaging ; wavelength selectivity</subject><ispartof>Angewandte Chemie International Edition, 2016-07, Vol.55 (31), p.8948-8952</ispartof><rights>2016 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5471-d090b98a156cc53565d1161da3a3424a185383130a46a2250f9d2aa6de0617423</citedby><cites>FETCH-LOGICAL-c5471-d090b98a156cc53565d1161da3a3424a185383130a46a2250f9d2aa6de0617423</cites><orcidid>0000-0003-3541-4548</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fanie.201603281$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fanie.201603281$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27294300$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fichte, Manuela A. H.</creatorcontrib><creatorcontrib>Weyel, Xenia M. M.</creatorcontrib><creatorcontrib>Junek, Stephan</creatorcontrib><creatorcontrib>Schäfer, Florian</creatorcontrib><creatorcontrib>Herbivo, Cyril</creatorcontrib><creatorcontrib>Goeldner, Maurice</creatorcontrib><creatorcontrib>Specht, Alexandre</creatorcontrib><creatorcontrib>Wachtveitl, Josef</creatorcontrib><creatorcontrib>Heckel, Alexander</creatorcontrib><title>Three-Dimensional Control of DNA Hybridization by Orthogonal Two-Color Two-Photon Uncaging</title><title>Angewandte Chemie International Edition</title><addtitle>Angew. Chem. Int. Ed</addtitle><description>We successfully introduced two‐photon‐sensitive photolabile groups ([7‐(diethylamino)coumarin‐4‐yl]methyl and p‐dialkylaminonitrobiphenyl) into DNA strands and demonstrated their suitability for three‐dimensional photorelease. To visualize the uncaging, we used a fluorescence readout based on double‐strand displacement in a hydrogel and in neurons. Orthogonal two‐photon uncaging of the two cages is possible, thus enabling complex scenarios of three‐dimensional control of hybridization with light.
Now also in 3D! Oligonucleotides with different sequences could be photoactivated with exact three‐dimensional control when different two‐photon‐sensitive photolabile groups were introduced into the strands. Uncaging was visualized by fluorescence as a result of double‐strand displacement. By using distinct irradiation conditions, either one of two sequences could be addressed selectively (see left‐hand images, which are overlayed on the right).</description><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>hydrogels</subject><subject>photochemistry</subject><subject>two-photon uncaging</subject><subject>wavelength selectivity</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqN0U1vEzEQBmALgWgpXDmilbhw2TBjr7-O0bY0laoUiVSgXixn10lcNuvW3qiEX4-blAhxgZNH8jOvNHoJeYswQgD60fbejSigAEYVPiPHyCmWTEr2PM8VY6VUHI_Iq5Rus1cKxEtyRCXVFQM4JjezVXSuPPVr1ycfetsVdeiHGLoiLIrT6biYbOfRt_6nHfJ3Md8WV3FYheWOzh5CWYcuxN30eRWGTK77xi59v3xNXixsl9ybp_eEXH86m9WT8vLq_KIeX5YNrySWLWiYa2WRi6bhjAveIgpsLbOsopVFxZliyMBWwlLKYaFbaq1oHQiUFWUn5MM-9y6G-41Lg1n71Lius70Lm2RQoUam8sH_QUGAFJqKTN__RW_DJuajd4pXTCPwrEZ71cSQUnQLcxf92satQTCPBZnHgsyhoLzw7il2M1-79sB_N5KB3oMH37ntP-LMeHpx9md4ud_1aXA_Drs2fjdCMsnN1-m5-aJvphP5rTbAfgF6Jaiw</recordid><startdate>20160725</startdate><enddate>20160725</enddate><creator>Fichte, Manuela A. H.</creator><creator>Weyel, Xenia M. M.</creator><creator>Junek, Stephan</creator><creator>Schäfer, Florian</creator><creator>Herbivo, Cyril</creator><creator>Goeldner, Maurice</creator><creator>Specht, Alexandre</creator><creator>Wachtveitl, Josef</creator><creator>Heckel, Alexander</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3541-4548</orcidid></search><sort><creationdate>20160725</creationdate><title>Three-Dimensional Control of DNA Hybridization by Orthogonal Two-Color Two-Photon Uncaging</title><author>Fichte, Manuela A. H. ; Weyel, Xenia M. M. ; Junek, Stephan ; Schäfer, Florian ; Herbivo, Cyril ; Goeldner, Maurice ; Specht, Alexandre ; Wachtveitl, Josef ; Heckel, Alexander</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5471-d090b98a156cc53565d1161da3a3424a185383130a46a2250f9d2aa6de0617423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>hydrogels</topic><topic>photochemistry</topic><topic>two-photon uncaging</topic><topic>wavelength selectivity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fichte, Manuela A. H.</creatorcontrib><creatorcontrib>Weyel, Xenia M. M.</creatorcontrib><creatorcontrib>Junek, Stephan</creatorcontrib><creatorcontrib>Schäfer, Florian</creatorcontrib><creatorcontrib>Herbivo, Cyril</creatorcontrib><creatorcontrib>Goeldner, Maurice</creatorcontrib><creatorcontrib>Specht, Alexandre</creatorcontrib><creatorcontrib>Wachtveitl, Josef</creatorcontrib><creatorcontrib>Heckel, Alexander</creatorcontrib><collection>Istex</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fichte, Manuela A. H.</au><au>Weyel, Xenia M. M.</au><au>Junek, Stephan</au><au>Schäfer, Florian</au><au>Herbivo, Cyril</au><au>Goeldner, Maurice</au><au>Specht, Alexandre</au><au>Wachtveitl, Josef</au><au>Heckel, Alexander</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Three-Dimensional Control of DNA Hybridization by Orthogonal Two-Color Two-Photon Uncaging</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angew. Chem. Int. Ed</addtitle><date>2016-07-25</date><risdate>2016</risdate><volume>55</volume><issue>31</issue><spage>8948</spage><epage>8952</epage><pages>8948-8952</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><coden>ACIEAY</coden><abstract>We successfully introduced two‐photon‐sensitive photolabile groups ([7‐(diethylamino)coumarin‐4‐yl]methyl and p‐dialkylaminonitrobiphenyl) into DNA strands and demonstrated their suitability for three‐dimensional photorelease. To visualize the uncaging, we used a fluorescence readout based on double‐strand displacement in a hydrogel and in neurons. Orthogonal two‐photon uncaging of the two cages is possible, thus enabling complex scenarios of three‐dimensional control of hybridization with light.
Now also in 3D! Oligonucleotides with different sequences could be photoactivated with exact three‐dimensional control when different two‐photon‐sensitive photolabile groups were introduced into the strands. Uncaging was visualized by fluorescence as a result of double‐strand displacement. By using distinct irradiation conditions, either one of two sequences could be addressed selectively (see left‐hand images, which are overlayed on the right).</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>27294300</pmid><doi>10.1002/anie.201603281</doi><tpages>5</tpages><edition>International ed. in English</edition><orcidid>https://orcid.org/0000-0003-3541-4548</orcidid></addata></record> |
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| subjects | Deoxyribonucleic acid DNA hydrogels photochemistry two-photon uncaging wavelength selectivity |
| title | Three-Dimensional Control of DNA Hybridization by Orthogonal Two-Color Two-Photon Uncaging |
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