Novel REIC/Dkk-3-encoding adenoviral vector as a promising therapeutic agent for pancreatic cancer
Reduced expression in immortalized cells (REIC)/dickkopf-3 (Dkk-3), a tumor suppressor gene, is downregulated in various cancers. We previously reported the tumor-inhibitory effects of the REIC/Dkk-3 gene, delivered by a conventional adenoviral vector (Ad-CAG-REIC) in pancreatic cancer. Here, we dev...
Gespeichert in:
| Veröffentlicht in: | Cancer gene therapy 2016-08, Vol.23 (8), p.278-283 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 283 |
|---|---|
| container_issue | 8 |
| container_start_page | 278 |
| container_title | Cancer gene therapy |
| container_volume | 23 |
| creator | Sawahara, H Shiraha, H Uchida, D Kato, H Nagahara, T Iwamuro, M Kataoka, J Horiguchi, S Watanabe, M Sakaguchi, M Takaki, A Nouso, K Nasu, Y Kumon, H Okada, H |
| description | Reduced expression in immortalized cells (REIC)/dickkopf-3 (Dkk-3), a tumor suppressor gene, is downregulated in various cancers. We previously reported the tumor-inhibitory effects of the
REIC/Dkk-3
gene, delivered by a conventional adenoviral vector (Ad-CAG-REIC) in pancreatic cancer. Here, we developed an Ad-REIC vector with a novel gene expression system, termed the super gene expression (SGE) system, and assessed its therapeutic effects relative to those of Ad-CAG-REIC in pancreatic cancer cells. Human pancreatic cancer cell lines ASPC1 and MIAPaCa2 were used. REIC/Dkk-3 expression was assessed by western blot analysis. Relative cell viability and apoptotic effects were examined
in vitro
. The anti-tumor effects of Ad-REIC treatment were assessed in the mouse xenograft model. Compared with Ad-CAG-REIC, Ad-SGE-REIC elicited a significant increase in REIC protein expression in the cells studied. Relative to Ad-CAG-REIC, Ad-SGE-REIC reduced cell viability and induced apoptosis in the ASPC1 and MIAPaCa2 cell lines
in vitro
, and achieved superior tumor growth inhibition in the mouse xenograft model. Compared with conventional Ad-REIC agents, Ad-SGE-REIC provided enhanced inhibitory effects against tumor growth. Our results indicate that Ad-SGE-REIC is an innovative therapeutic tool for pancreatic cancer. |
| doi_str_mv | 10.1038/cgt.2016.31 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1819137425</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A461583972</galeid><sourcerecordid>A461583972</sourcerecordid><originalsourceid>FETCH-LOGICAL-c550t-137b76a0da51407f5e32353e1900b2890bc95c6d28705f0f35e8cb6b4ce1ffda3</originalsourceid><addsrcrecordid>eNqNkl1rFDEUhoModq1eeS8DghR0tvmYfMxl2VYtFAXR65DJnMxOO5usycyC_94M29a2lCK5SDjnyXvOSV6E3hK8JJipY9uNS4qJWDLyDC1IJUXJOcbP0QLXtC5JjdkBepXSJcY5KdlLdEBlJZTCaoGab2EHQ_Hj7Hx1fHp1VbISvA1t77vCtODDro9mKHZgxxALkwpTbGPY9GkGxjVEs4Vp7G1hOvBj4TK0Nd5GMHPQ5iPE1-iFM0OCN9f7Ifr1-ezn6mt58f3L-erkorS53bEkTDZSGNwaTiosHQdGGWeQ-8cNVTVubM2taKmSmDvsGAdlG9FUFohzrWGH6Givmzv8PUEade7TwjAYD2FKmihS5yIV5f-DVkLi_EYZff8AvQxT9HkQTUVFJOUiqz5BzVqE10rwf1RnBtC9d2GMxs6l9UklCFesljRTy0eovFrY9DZ4cH2O37vw4c6FNZhhXKcw5H8JPt0HP-5BG0NKEZzexn5j4h9NsJ69pLOX9OwlzeaZ3l3PNDUbaG_ZG_Nk4NMeSDnlO4h3hn5E7y-bV85M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1814159865</pqid></control><display><type>article</type><title>Novel REIC/Dkk-3-encoding adenoviral vector as a promising therapeutic agent for pancreatic cancer</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Sawahara, H ; Shiraha, H ; Uchida, D ; Kato, H ; Nagahara, T ; Iwamuro, M ; Kataoka, J ; Horiguchi, S ; Watanabe, M ; Sakaguchi, M ; Takaki, A ; Nouso, K ; Nasu, Y ; Kumon, H ; Okada, H</creator><creatorcontrib>Sawahara, H ; Shiraha, H ; Uchida, D ; Kato, H ; Nagahara, T ; Iwamuro, M ; Kataoka, J ; Horiguchi, S ; Watanabe, M ; Sakaguchi, M ; Takaki, A ; Nouso, K ; Nasu, Y ; Kumon, H ; Okada, H</creatorcontrib><description>Reduced expression in immortalized cells (REIC)/dickkopf-3 (Dkk-3), a tumor suppressor gene, is downregulated in various cancers. We previously reported the tumor-inhibitory effects of the
REIC/Dkk-3
gene, delivered by a conventional adenoviral vector (Ad-CAG-REIC) in pancreatic cancer. Here, we developed an Ad-REIC vector with a novel gene expression system, termed the super gene expression (SGE) system, and assessed its therapeutic effects relative to those of Ad-CAG-REIC in pancreatic cancer cells. Human pancreatic cancer cell lines ASPC1 and MIAPaCa2 were used. REIC/Dkk-3 expression was assessed by western blot analysis. Relative cell viability and apoptotic effects were examined
in vitro
. The anti-tumor effects of Ad-REIC treatment were assessed in the mouse xenograft model. Compared with Ad-CAG-REIC, Ad-SGE-REIC elicited a significant increase in REIC protein expression in the cells studied. Relative to Ad-CAG-REIC, Ad-SGE-REIC reduced cell viability and induced apoptosis in the ASPC1 and MIAPaCa2 cell lines
in vitro
, and achieved superior tumor growth inhibition in the mouse xenograft model. Compared with conventional Ad-REIC agents, Ad-SGE-REIC provided enhanced inhibitory effects against tumor growth. Our results indicate that Ad-SGE-REIC is an innovative therapeutic tool for pancreatic cancer.</description><identifier>ISSN: 0929-1903</identifier><identifier>EISSN: 1476-5500</identifier><identifier>DOI: 10.1038/cgt.2016.31</identifier><identifier>PMID: 27468808</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>631/67/1504/1713 ; 64/110 ; Adenoviridae - genetics ; Analysis ; Animals ; Apoptosis ; Apoptosis - genetics ; Biomedical and Life Sciences ; Biomedicine ; Cancer cells ; Care and treatment ; Cell Line, Tumor ; Cell Survival - genetics ; Cell viability ; Chemotherapy ; Diagnosis ; Disease Models, Animal ; Gene Expression ; Gene Order ; Gene Therapy ; Genetic Therapy ; Genetic Vectors - administration & dosage ; Genetic Vectors - genetics ; Health aspects ; Humans ; Intercellular Signaling Peptides and Proteins - genetics ; Intercellular Signaling Peptides and Proteins - metabolism ; JNK Mitogen-Activated Protein Kinases - metabolism ; Kinases ; Medical innovations ; Mice ; original-article ; Pancreatic cancer ; Pancreatic Neoplasms - genetics ; Pancreatic Neoplasms - metabolism ; Pancreatic Neoplasms - pathology ; Pancreatic Neoplasms - therapy ; Signal Transduction ; Trinucleotide repeats ; Tumor Burden - genetics ; Tumor cell lines ; Tumor suppressor genes ; Tumors ; Xenograft Model Antitumor Assays ; Xenografts</subject><ispartof>Cancer gene therapy, 2016-08, Vol.23 (8), p.278-283</ispartof><rights>Nature America, Inc., part of Springer Nature. 2016</rights><rights>COPYRIGHT 2016 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Aug 2016</rights><rights>Nature America, Inc., part of Springer Nature. 2016.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-137b76a0da51407f5e32353e1900b2890bc95c6d28705f0f35e8cb6b4ce1ffda3</citedby><cites>FETCH-LOGICAL-c550t-137b76a0da51407f5e32353e1900b2890bc95c6d28705f0f35e8cb6b4ce1ffda3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/cgt.2016.31$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/cgt.2016.31$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27468808$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sawahara, H</creatorcontrib><creatorcontrib>Shiraha, H</creatorcontrib><creatorcontrib>Uchida, D</creatorcontrib><creatorcontrib>Kato, H</creatorcontrib><creatorcontrib>Nagahara, T</creatorcontrib><creatorcontrib>Iwamuro, M</creatorcontrib><creatorcontrib>Kataoka, J</creatorcontrib><creatorcontrib>Horiguchi, S</creatorcontrib><creatorcontrib>Watanabe, M</creatorcontrib><creatorcontrib>Sakaguchi, M</creatorcontrib><creatorcontrib>Takaki, A</creatorcontrib><creatorcontrib>Nouso, K</creatorcontrib><creatorcontrib>Nasu, Y</creatorcontrib><creatorcontrib>Kumon, H</creatorcontrib><creatorcontrib>Okada, H</creatorcontrib><title>Novel REIC/Dkk-3-encoding adenoviral vector as a promising therapeutic agent for pancreatic cancer</title><title>Cancer gene therapy</title><addtitle>Cancer Gene Ther</addtitle><addtitle>Cancer Gene Ther</addtitle><description>Reduced expression in immortalized cells (REIC)/dickkopf-3 (Dkk-3), a tumor suppressor gene, is downregulated in various cancers. We previously reported the tumor-inhibitory effects of the
REIC/Dkk-3
gene, delivered by a conventional adenoviral vector (Ad-CAG-REIC) in pancreatic cancer. Here, we developed an Ad-REIC vector with a novel gene expression system, termed the super gene expression (SGE) system, and assessed its therapeutic effects relative to those of Ad-CAG-REIC in pancreatic cancer cells. Human pancreatic cancer cell lines ASPC1 and MIAPaCa2 were used. REIC/Dkk-3 expression was assessed by western blot analysis. Relative cell viability and apoptotic effects were examined
in vitro
. The anti-tumor effects of Ad-REIC treatment were assessed in the mouse xenograft model. Compared with Ad-CAG-REIC, Ad-SGE-REIC elicited a significant increase in REIC protein expression in the cells studied. Relative to Ad-CAG-REIC, Ad-SGE-REIC reduced cell viability and induced apoptosis in the ASPC1 and MIAPaCa2 cell lines
in vitro
, and achieved superior tumor growth inhibition in the mouse xenograft model. Compared with conventional Ad-REIC agents, Ad-SGE-REIC provided enhanced inhibitory effects against tumor growth. Our results indicate that Ad-SGE-REIC is an innovative therapeutic tool for pancreatic cancer.</description><subject>631/67/1504/1713</subject><subject>64/110</subject><subject>Adenoviridae - genetics</subject><subject>Analysis</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer cells</subject><subject>Care and treatment</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - genetics</subject><subject>Cell viability</subject><subject>Chemotherapy</subject><subject>Diagnosis</subject><subject>Disease Models, Animal</subject><subject>Gene Expression</subject><subject>Gene Order</subject><subject>Gene Therapy</subject><subject>Genetic Therapy</subject><subject>Genetic Vectors - administration & dosage</subject><subject>Genetic Vectors - genetics</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Intercellular Signaling Peptides and Proteins - genetics</subject><subject>Intercellular Signaling Peptides and Proteins - metabolism</subject><subject>JNK Mitogen-Activated Protein Kinases - metabolism</subject><subject>Kinases</subject><subject>Medical innovations</subject><subject>Mice</subject><subject>original-article</subject><subject>Pancreatic cancer</subject><subject>Pancreatic Neoplasms - genetics</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>Pancreatic Neoplasms - therapy</subject><subject>Signal Transduction</subject><subject>Trinucleotide repeats</subject><subject>Tumor Burden - genetics</subject><subject>Tumor cell lines</subject><subject>Tumor suppressor genes</subject><subject>Tumors</subject><subject>Xenograft Model Antitumor Assays</subject><subject>Xenografts</subject><issn>0929-1903</issn><issn>1476-5500</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkl1rFDEUhoModq1eeS8DghR0tvmYfMxl2VYtFAXR65DJnMxOO5usycyC_94M29a2lCK5SDjnyXvOSV6E3hK8JJipY9uNS4qJWDLyDC1IJUXJOcbP0QLXtC5JjdkBepXSJcY5KdlLdEBlJZTCaoGab2EHQ_Hj7Hx1fHp1VbISvA1t77vCtODDro9mKHZgxxALkwpTbGPY9GkGxjVEs4Vp7G1hOvBj4TK0Nd5GMHPQ5iPE1-iFM0OCN9f7Ifr1-ezn6mt58f3L-erkorS53bEkTDZSGNwaTiosHQdGGWeQ-8cNVTVubM2taKmSmDvsGAdlG9FUFohzrWGH6Givmzv8PUEade7TwjAYD2FKmihS5yIV5f-DVkLi_EYZff8AvQxT9HkQTUVFJOUiqz5BzVqE10rwf1RnBtC9d2GMxs6l9UklCFesljRTy0eovFrY9DZ4cH2O37vw4c6FNZhhXKcw5H8JPt0HP-5BG0NKEZzexn5j4h9NsJ69pLOX9OwlzeaZ3l3PNDUbaG_ZG_Nk4NMeSDnlO4h3hn5E7y-bV85M</recordid><startdate>20160801</startdate><enddate>20160801</enddate><creator>Sawahara, H</creator><creator>Shiraha, H</creator><creator>Uchida, D</creator><creator>Kato, H</creator><creator>Nagahara, T</creator><creator>Iwamuro, M</creator><creator>Kataoka, J</creator><creator>Horiguchi, S</creator><creator>Watanabe, M</creator><creator>Sakaguchi, M</creator><creator>Takaki, A</creator><creator>Nouso, K</creator><creator>Nasu, Y</creator><creator>Kumon, H</creator><creator>Okada, H</creator><general>Nature Publishing Group US</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20160801</creationdate><title>Novel REIC/Dkk-3-encoding adenoviral vector as a promising therapeutic agent for pancreatic cancer</title><author>Sawahara, H ; Shiraha, H ; Uchida, D ; Kato, H ; Nagahara, T ; Iwamuro, M ; Kataoka, J ; Horiguchi, S ; Watanabe, M ; Sakaguchi, M ; Takaki, A ; Nouso, K ; Nasu, Y ; Kumon, H ; Okada, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c550t-137b76a0da51407f5e32353e1900b2890bc95c6d28705f0f35e8cb6b4ce1ffda3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>631/67/1504/1713</topic><topic>64/110</topic><topic>Adenoviridae - genetics</topic><topic>Analysis</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer cells</topic><topic>Care and treatment</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - genetics</topic><topic>Cell viability</topic><topic>Chemotherapy</topic><topic>Diagnosis</topic><topic>Disease Models, Animal</topic><topic>Gene Expression</topic><topic>Gene Order</topic><topic>Gene Therapy</topic><topic>Genetic Therapy</topic><topic>Genetic Vectors - administration & dosage</topic><topic>Genetic Vectors - genetics</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Intercellular Signaling Peptides and Proteins - genetics</topic><topic>Intercellular Signaling Peptides and Proteins - metabolism</topic><topic>JNK Mitogen-Activated Protein Kinases - metabolism</topic><topic>Kinases</topic><topic>Medical innovations</topic><topic>Mice</topic><topic>original-article</topic><topic>Pancreatic cancer</topic><topic>Pancreatic Neoplasms - genetics</topic><topic>Pancreatic Neoplasms - metabolism</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>Pancreatic Neoplasms - therapy</topic><topic>Signal Transduction</topic><topic>Trinucleotide repeats</topic><topic>Tumor Burden - genetics</topic><topic>Tumor cell lines</topic><topic>Tumor suppressor genes</topic><topic>Tumors</topic><topic>Xenograft Model Antitumor Assays</topic><topic>Xenografts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sawahara, H</creatorcontrib><creatorcontrib>Shiraha, H</creatorcontrib><creatorcontrib>Uchida, D</creatorcontrib><creatorcontrib>Kato, H</creatorcontrib><creatorcontrib>Nagahara, T</creatorcontrib><creatorcontrib>Iwamuro, M</creatorcontrib><creatorcontrib>Kataoka, J</creatorcontrib><creatorcontrib>Horiguchi, S</creatorcontrib><creatorcontrib>Watanabe, M</creatorcontrib><creatorcontrib>Sakaguchi, M</creatorcontrib><creatorcontrib>Takaki, A</creatorcontrib><creatorcontrib>Nouso, K</creatorcontrib><creatorcontrib>Nasu, Y</creatorcontrib><creatorcontrib>Kumon, H</creatorcontrib><creatorcontrib>Okada, H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Cancer gene therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sawahara, H</au><au>Shiraha, H</au><au>Uchida, D</au><au>Kato, H</au><au>Nagahara, T</au><au>Iwamuro, M</au><au>Kataoka, J</au><au>Horiguchi, S</au><au>Watanabe, M</au><au>Sakaguchi, M</au><au>Takaki, A</au><au>Nouso, K</au><au>Nasu, Y</au><au>Kumon, H</au><au>Okada, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel REIC/Dkk-3-encoding adenoviral vector as a promising therapeutic agent for pancreatic cancer</atitle><jtitle>Cancer gene therapy</jtitle><stitle>Cancer Gene Ther</stitle><addtitle>Cancer Gene Ther</addtitle><date>2016-08-01</date><risdate>2016</risdate><volume>23</volume><issue>8</issue><spage>278</spage><epage>283</epage><pages>278-283</pages><issn>0929-1903</issn><eissn>1476-5500</eissn><abstract>Reduced expression in immortalized cells (REIC)/dickkopf-3 (Dkk-3), a tumor suppressor gene, is downregulated in various cancers. We previously reported the tumor-inhibitory effects of the
REIC/Dkk-3
gene, delivered by a conventional adenoviral vector (Ad-CAG-REIC) in pancreatic cancer. Here, we developed an Ad-REIC vector with a novel gene expression system, termed the super gene expression (SGE) system, and assessed its therapeutic effects relative to those of Ad-CAG-REIC in pancreatic cancer cells. Human pancreatic cancer cell lines ASPC1 and MIAPaCa2 were used. REIC/Dkk-3 expression was assessed by western blot analysis. Relative cell viability and apoptotic effects were examined
in vitro
. The anti-tumor effects of Ad-REIC treatment were assessed in the mouse xenograft model. Compared with Ad-CAG-REIC, Ad-SGE-REIC elicited a significant increase in REIC protein expression in the cells studied. Relative to Ad-CAG-REIC, Ad-SGE-REIC reduced cell viability and induced apoptosis in the ASPC1 and MIAPaCa2 cell lines
in vitro
, and achieved superior tumor growth inhibition in the mouse xenograft model. Compared with conventional Ad-REIC agents, Ad-SGE-REIC provided enhanced inhibitory effects against tumor growth. Our results indicate that Ad-SGE-REIC is an innovative therapeutic tool for pancreatic cancer.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>27468808</pmid><doi>10.1038/cgt.2016.31</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0929-1903 |
| ispartof | Cancer gene therapy, 2016-08, Vol.23 (8), p.278-283 |
| issn | 0929-1903 1476-5500 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1819137425 |
| source | MEDLINE; SpringerLink Journals |
| subjects | 631/67/1504/1713 64/110 Adenoviridae - genetics Analysis Animals Apoptosis Apoptosis - genetics Biomedical and Life Sciences Biomedicine Cancer cells Care and treatment Cell Line, Tumor Cell Survival - genetics Cell viability Chemotherapy Diagnosis Disease Models, Animal Gene Expression Gene Order Gene Therapy Genetic Therapy Genetic Vectors - administration & dosage Genetic Vectors - genetics Health aspects Humans Intercellular Signaling Peptides and Proteins - genetics Intercellular Signaling Peptides and Proteins - metabolism JNK Mitogen-Activated Protein Kinases - metabolism Kinases Medical innovations Mice original-article Pancreatic cancer Pancreatic Neoplasms - genetics Pancreatic Neoplasms - metabolism Pancreatic Neoplasms - pathology Pancreatic Neoplasms - therapy Signal Transduction Trinucleotide repeats Tumor Burden - genetics Tumor cell lines Tumor suppressor genes Tumors Xenograft Model Antitumor Assays Xenografts |
| title | Novel REIC/Dkk-3-encoding adenoviral vector as a promising therapeutic agent for pancreatic cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T12%3A29%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Novel%20REIC/Dkk-3-encoding%20adenoviral%20vector%20as%20a%20promising%20therapeutic%20agent%20for%20pancreatic%20cancer&rft.jtitle=Cancer%20gene%20therapy&rft.au=Sawahara,%20H&rft.date=2016-08-01&rft.volume=23&rft.issue=8&rft.spage=278&rft.epage=283&rft.pages=278-283&rft.issn=0929-1903&rft.eissn=1476-5500&rft_id=info:doi/10.1038/cgt.2016.31&rft_dat=%3Cgale_proqu%3EA461583972%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1814159865&rft_id=info:pmid/27468808&rft_galeid=A461583972&rfr_iscdi=true |