Internalization of Vectored Liposomes in a Culture of Poorly Differentiated Tumor Cells
Internalization of liposomal nanocontainers conjugated with monoclonal antibodies to VEGF, VEGFR2 (KDR), and proteins overproduced in the tumor tissue was studied in vitro on cultures of poorly differentiated tumor cells. Comparative analysis of accumulation of vectored liposomes in the tumor cells...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2016-08, Vol.161 (4), p.593-599 |
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| container_title | Bulletin of experimental biology and medicine |
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| creator | Mel’nikov, P. A. Baklaushev, V. P. Gabashvili, A. N. Nukolova, N. V. Levinsky, A. B. Chehonin, V. P. |
| description | Internalization of liposomal nanocontainers conjugated with monoclonal antibodies to VEGF, VEGFR2 (KDR), and proteins overproduced in the tumor tissue was studied
in vitro
on cultures of poorly differentiated tumor cells. Comparative analysis of accumulation of vectored liposomes in the tumor cells was performed by evaluating co-localization of labeled containers and cell organelles by laser scanning confocal microscopy. We observed nearly 2 times more active penetration and accumulation of liposomes vectored with antibodies in the tumor cells in comparison with non-vectored liposomes. Selective clathrin-dependent penetration of vectored liposomes into tumor cells was demonstrated by using pharmacological agents inhibiting endocytosis. |
| doi_str_mv | 10.1007/s10517-016-3466-3 |
| format | Article |
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in vitro
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in vitro
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Selective clathrin-dependent penetration of vectored liposomes into tumor cells was demonstrated by using pharmacological agents inhibiting endocytosis.</description><subject>Analysis</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cell Line, Tumor</subject><subject>Glioma - metabolism</subject><subject>Gliomas</subject><subject>Internal Medicine</subject><subject>Laboratory Medicine</subject><subject>Liposomes - chemistry</subject><subject>Liposomes - metabolism</subject><subject>Microscopy, Confocal</subject><subject>Monoclonal antibodies</subject><subject>Pathology</subject><subject>Proteins</subject><subject>Rats</subject><subject>Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - immunology</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kVtrFTEUhYMo9lj9Ab7IgCC-TE0mM7k8luOtcEAfqj6GNLPTpmSSY5J5qL_ehFNLK0og128t9s5C6CXBJwRj_i4TPBHeY8J6OrI6PUIbMnHai2Egj9EGV6gfhRBH6FnO1-2IGXmKjgY-ScwZ26AfZ6FACtq7X7q4GLpou-9gSkwwdzu3jzkukDsXOt1tV1_WBA35GmPyN917Zy0kCMXpUvnzdYmp24L3-Tl6YrXP8OJ2PUbfPn44337ud18-nW1Pd70Z5VB6SzmAmKXVgKXFmAphmBGaswuoG2vmkfERhJSTtNzMA5kowzMfuWBSDpYeo7cH332KP1fIRS0um1qBDhDXrIggglLJOK_o67_Q67i21hvFsJBMVvSOutQelAs2lqRNM1WnoxSToKNoXif_oOqYYXEmBrCu3j8QvLknuALty1WOfm1_nh-C5ACaFHNOYNU-uUWnG0WwaqmrQ-qqpq5a6qrV_Oq2s_VigflO8SfmCgwHINencAnpXuv_df0NMfG0yA</recordid><startdate>20160801</startdate><enddate>20160801</enddate><creator>Mel’nikov, P. 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in vitro
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| subjects | Analysis Animals Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Biomedical and Life Sciences Biomedicine Cell Biology Cell Line, Tumor Glioma - metabolism Gliomas Internal Medicine Laboratory Medicine Liposomes - chemistry Liposomes - metabolism Microscopy, Confocal Monoclonal antibodies Pathology Proteins Rats Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine) Vascular endothelial growth factor Vascular Endothelial Growth Factor A - immunology |
| title | Internalization of Vectored Liposomes in a Culture of Poorly Differentiated Tumor Cells |
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