Generation and characterization of human B lymphocyte stimulator blocking monoclonal antibody

•A new humanized anti-Blys antibody was developed.•The antibody binds with sub-nanomolar affinity and high specificity.•The antibody significantly inhibited Blys induced B cell proliferation, in vivo. The cytokine, B lymphocyte stimulator (Blys) is essential for activation and proliferation of B cel...

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Veröffentlicht in:Molecular immunology 2016-09, Vol.77, p.141-147
Hauptverfasser: Zhuang, Weiliang, Zhang, Jianjun, Pei, Lili, Fang, Shuping, Liu, Honghao, Wang, Ruixue, Su, Yunpeng
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container_end_page 147
container_issue
container_start_page 141
container_title Molecular immunology
container_volume 77
creator Zhuang, Weiliang
Zhang, Jianjun
Pei, Lili
Fang, Shuping
Liu, Honghao
Wang, Ruixue
Su, Yunpeng
description •A new humanized anti-Blys antibody was developed.•The antibody binds with sub-nanomolar affinity and high specificity.•The antibody significantly inhibited Blys induced B cell proliferation, in vivo. The cytokine, B lymphocyte stimulator (Blys) is essential for activation and proliferation of B cells and is involved in the pathogenesis of B-cell mediated autoimmune diseases. Based on its essential activity, Blys may be a potential therapeutic target for human autoimmune diseases. In this article, we have described the development of a novel humanized anti-Blys antibody, NMB04, that binds with high affinity and specificity to both soluble and membrane bound Blys. This monoclonal antibody has the potential to block Blys binding to all its three receptors, TACI, BCMA and BR-3. Further in vivo studies revealed that NMB04 possessed more potent inhibitory activity against human Blys as compared to an existing antibody, Belimumab. Therefore, NMB04 may have potential as a therapeutic candidate targeting autoimmune diseases.
doi_str_mv 10.1016/j.molimm.2016.08.002
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The cytokine, B lymphocyte stimulator (Blys) is essential for activation and proliferation of B cells and is involved in the pathogenesis of B-cell mediated autoimmune diseases. Based on its essential activity, Blys may be a potential therapeutic target for human autoimmune diseases. In this article, we have described the development of a novel humanized anti-Blys antibody, NMB04, that binds with high affinity and specificity to both soluble and membrane bound Blys. This monoclonal antibody has the potential to block Blys binding to all its three receptors, TACI, BCMA and BR-3. Further in vivo studies revealed that NMB04 possessed more potent inhibitory activity against human Blys as compared to an existing antibody, Belimumab. 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subjects Animals
Antibodies, Monoclonal - immunology
Antibodies, Monoclonal - pharmacology
Antibody
Antibody Affinity - immunology
Antibody Specificity - immunology
Autoimmune
B-Cell Activating Factor - immunology
B-Lymphocytes - drug effects
B-Lymphocytes - immunology
Blys
Enzyme-Linked Immunosorbent Assay
Female
Humans
Lymphocyte Activation - drug effects
Lymphocyte Activation - immunology
Macaca fascicularis
Male
Mice
Mice, Inbred BALB C
Polymerase Chain Reaction
SLE
title Generation and characterization of human B lymphocyte stimulator blocking monoclonal antibody
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