Hydrodynamic Tail Vein Injection as a Simple Tool for Yielding Extended Transgene Expression in Solid Tumors
Hydrodynamic tail vein injection was considered an in vivo transfection method that yields a higher level of gene expression mainly in the liver. This method has been applied to cancer gene therapy targeting both hepatic and non-hepatic cancers. However, intratumor transgene expression in non-hepati...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2016/09/01, Vol.39(9), pp.1555-1558 |
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| container_issue | 9 |
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| container_title | Biological & pharmaceutical bulletin |
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| creator | Takayama, Takuma Ukawa, Masami Kanazawa, Yuki Ando, Hidenori Shimizu, Taro Ishida, Tatsuhiro |
| description | Hydrodynamic tail vein injection was considered an in vivo transfection method that yields a higher level of gene expression mainly in the liver. This method has been applied to cancer gene therapy targeting both hepatic and non-hepatic cancers. However, intratumor transgene expression in non-hepatic tumors has not been well studied. In this study, we showed an extended transgene expression of β-galactosidase (LacZ), a nonsecretory protein, in a subcutaneously implanted murine solid tumor following the hydrodynamic injection of plasmid DNA (LacZ pDNA). Our result may indicate that the hydrodynamic injection method is a powerful tool that can be used to gain transgene expression not only in the liver but also in solid tumors. |
| doi_str_mv | 10.1248/bpb.b16-00283 |
| format | Article |
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This method has been applied to cancer gene therapy targeting both hepatic and non-hepatic cancers. However, intratumor transgene expression in non-hepatic tumors has not been well studied. In this study, we showed an extended transgene expression of β-galactosidase (LacZ), a nonsecretory protein, in a subcutaneously implanted murine solid tumor following the hydrodynamic injection of plasmid DNA (LacZ pDNA). 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Our result may indicate that the hydrodynamic injection method is a powerful tool that can be used to gain transgene expression not only in the liver but also in solid tumors.</description><subject>Animals</subject><subject>beta-Galactosidase - genetics</subject><subject>beta-Galactosidase - metabolism</subject><subject>Cell Line, Tumor</subject><subject>DNA - administration & dosage</subject><subject>gene delivery</subject><subject>Gene Expression</subject><subject>Gene Transfer Techniques</subject><subject>Genetic Therapy - methods</subject><subject>hydrodynamic injection</subject><subject>Injections, Intravenous</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Neoplasms - metabolism</subject><subject>plasmid DNA</subject><subject>Plasmids</subject><subject>Tail</subject><subject>Transgenes - genetics</subject><subject>tumor allograft</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMFv2yAUh9G0as26HXedOO7i9gG2wccp6tpKlXZoNmknhPFzRoQhA1ta_vuRpMvlIfE-vvf4EfKJwS3jtbrr9_1tz9oKgCvxhqyYqGXVcNa8JSvomKpa1qhr8j7nHQBI4OIdueayUVyIZkX842FIcTgEMzlLN8Z5-hNdoE9hh3Z2MVCTqaEvbtp7pJsYPR1jor8c-sGFLb3_O2MYcKCbZELeYsBytU-Y8_FtEb1E70p3mWLKH8jVaHzGj6_nDfnx7X6zfqyevz88rb8-V7aV9VwZZhiXYsTejBxRdYNomG25rGFoFVOq7gdQhgsDYDvZQ816I2HgDC23KMUN-XL27lP8s2Ce9eSyRe9NwLhkzRRrW9FCXRe0OqM2xZwTjnqf3GTSQTPQx4B1CViXgPUp4MJ_flUv_YTDhf6faAEezkDpOmt8DN4F1Lu4pFD-rG2WvYs-ag4nqeig08C5Zk3THIvikregVDGtz6Zdns0WL6NMmp31eFpMdLo7lsuCl679bZLGIP4BKPiljQ</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Takayama, Takuma</creator><creator>Ukawa, Masami</creator><creator>Kanazawa, Yuki</creator><creator>Ando, Hidenori</creator><creator>Shimizu, Taro</creator><creator>Ishida, Tatsuhiro</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2016</creationdate><title>Hydrodynamic Tail Vein Injection as a Simple Tool for Yielding Extended Transgene Expression in Solid Tumors</title><author>Takayama, Takuma ; Ukawa, Masami ; Kanazawa, Yuki ; Ando, Hidenori ; Shimizu, Taro ; Ishida, Tatsuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c674t-a1a1273febaf2ee89d351c62740d681884bd08a23a00c97b041ba70d21ec2ce73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>beta-Galactosidase - genetics</topic><topic>beta-Galactosidase - metabolism</topic><topic>Cell Line, Tumor</topic><topic>DNA - administration & dosage</topic><topic>gene delivery</topic><topic>Gene Expression</topic><topic>Gene Transfer Techniques</topic><topic>Genetic Therapy - methods</topic><topic>hydrodynamic injection</topic><topic>Injections, Intravenous</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Neoplasms - metabolism</topic><topic>plasmid DNA</topic><topic>Plasmids</topic><topic>Tail</topic><topic>Transgenes - genetics</topic><topic>tumor allograft</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takayama, Takuma</creatorcontrib><creatorcontrib>Ukawa, Masami</creatorcontrib><creatorcontrib>Kanazawa, Yuki</creatorcontrib><creatorcontrib>Ando, Hidenori</creatorcontrib><creatorcontrib>Shimizu, Taro</creatorcontrib><creatorcontrib>Ishida, Tatsuhiro</creatorcontrib><creatorcontrib>Tokushima University</creatorcontrib><creatorcontrib>bDepartment of Cancer Metabolism and Therapy</creatorcontrib><creatorcontrib>aDepartment of Pharmacokinetics and Biopharmaceutics</creatorcontrib><creatorcontrib>Subdivision of Biopharmaceutical Sciences</creatorcontrib><creatorcontrib>Institute of Biomedical Sciences</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takayama, Takuma</au><au>Ukawa, Masami</au><au>Kanazawa, Yuki</au><au>Ando, Hidenori</au><au>Shimizu, Taro</au><au>Ishida, Tatsuhiro</au><aucorp>Tokushima University</aucorp><aucorp>bDepartment of Cancer Metabolism and Therapy</aucorp><aucorp>aDepartment of Pharmacokinetics and Biopharmaceutics</aucorp><aucorp>Subdivision of Biopharmaceutical Sciences</aucorp><aucorp>Institute of Biomedical Sciences</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrodynamic Tail Vein Injection as a Simple Tool for Yielding Extended Transgene Expression in Solid Tumors</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2016</date><risdate>2016</risdate><volume>39</volume><issue>9</issue><spage>1555</spage><epage>1558</epage><pages>1555-1558</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>Hydrodynamic tail vein injection was considered an in vivo transfection method that yields a higher level of gene expression mainly in the liver. This method has been applied to cancer gene therapy targeting both hepatic and non-hepatic cancers. However, intratumor transgene expression in non-hepatic tumors has not been well studied. In this study, we showed an extended transgene expression of β-galactosidase (LacZ), a nonsecretory protein, in a subcutaneously implanted murine solid tumor following the hydrodynamic injection of plasmid DNA (LacZ pDNA). Our result may indicate that the hydrodynamic injection method is a powerful tool that can be used to gain transgene expression not only in the liver but also in solid tumors.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>27582335</pmid><doi>10.1248/bpb.b16-00283</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Animals beta-Galactosidase - genetics beta-Galactosidase - metabolism Cell Line, Tumor DNA - administration & dosage gene delivery Gene Expression Gene Transfer Techniques Genetic Therapy - methods hydrodynamic injection Injections, Intravenous Liver - metabolism Male Mice Mice, Inbred BALB C Neoplasms - metabolism plasmid DNA Plasmids Tail Transgenes - genetics tumor allograft |
| title | Hydrodynamic Tail Vein Injection as a Simple Tool for Yielding Extended Transgene Expression in Solid Tumors |
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