Combinatorial alanine-scanning

Combinatorial alanine-scanning

Combinatorial libraries of alanine-substituted proteins can be used to rapidly identify residues important for protein function, stability and shape. Each alanine substitution examines the contribution of an individual amino acid sidechain to the functionality of the protein. The recently described...

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Veröffentlicht in:Current Opinion in Chemical Biology 2001-06, Vol.5 (3), p.302-307
Hauptverfasser: Morrison, Kim L, Weiss, Gregory A
Format: Artikel
Sprache:eng
Schlagworte:
alanine
Alanine - chemistry
Amino Acid Substitution
binomial mutagenesis
Combinatorial alanine mutagenesis
combinatorial chemistry
Combinatorial Chemistry Techniques
functional epitopes
hot spot
Models, Molecular
Mutagenesis
receptor-ligand interactions
shotgun scanning
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Zusammenfassung:Combinatorial libraries of alanine-substituted proteins can be used to rapidly identify residues important for protein function, stability and shape. Each alanine substitution examines the contribution of an individual amino acid sidechain to the functionality of the protein. The recently described method of shotgun scanning uses phage-displayed libraries of alanine-substituted proteins for high-throughput analysis.
ISSN:1367-5931
1879-0402
DOI:10.1016/S1367-5931(00)00206-4