Synthesis of l- and d-Ubiquitin by One-Pot Ligation and Metal-Free Desulfurization

Native chemical ligation combined with desulfurization has become a powerful strategy for the chemical synthesis of proteins. Here we describe the use of a new thiol additive, methyl thioglycolate, to accomplish one‐pot native chemical ligation and metal‐free desulfurization for chemical protein syn...

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Veröffentlicht in:	Chemistry : a European journal 2016-05, Vol.22 (22), p.7623-7628
Hauptverfasser:	Huang , Yi-Chao, Chen , Chen-Chen, Gao, Shuai, Wang, Ye-Hai, Xiao, Hua, Wang, Feng, Tian, Chang-Lin, Li, Yi-Ming
Format:	Artikel
Sprache:	eng
Schlagworte:	Additives Chemical synthesis Chemistry Circular dichroism Circularity Coupling (molecular) Crystallography Crystallography, X-Ray Desulfurization Desulfurizing Dichroism Fluorenes - chemistry Folding Ligation Metals methyl thioglycolate Molecular Conformation native chemical ligation Peptide synthesis Peptides Peptides - chemistry Protein biosynthesis Protein folding Protein synthesis Proteins Purity Segments Solid-Phase Synthesis Techniques Spectroscopy Strategy Sulfhydryl Compounds - chemistry Synthesis (chemistry) Thiols Ubiquitin Ubiquitin - chemical synthesis Ubiquitin - chemistry X-ray crystallography X-ray diffraction
Online-Zugang:	Volltext
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