Improved sensitivity of lateral flow assay using paper-based sample concentration technique
Lateral flow assays (LFAs) hold great promise for point-of-care testing, especially in resource-poor settings. However, the poor sensitivity of LFAs limits their widespread applications. To address this, we developed a novel device by integrating dialysis-based concentration method into LFAs. The de...
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| Veröffentlicht in: | Talanta (Oxford) 2016-05, Vol.152, p.269-276 |
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| creator | Tang, Ruihua Yang, Hui Choi, Jane Ru Gong, Yan Hu, Jie Feng, Shangsheng Pingguan-Murphy, Belinda Mei, Qibing Xu, Feng |
| description | Lateral flow assays (LFAs) hold great promise for point-of-care testing, especially in resource-poor settings. However, the poor sensitivity of LFAs limits their widespread applications. To address this, we developed a novel device by integrating dialysis-based concentration method into LFAs. The device successfully achieved 10-fold signal enhancement in Human Immunodeficiency Virus (HIV) nucleic acid detection with a detection limit of 0.1nM and 4-fold signal enhancement in myoglobin (MYO) detection with a detection limit of 1.56ng/mL in less than 25min. This simple, low-cost and portable integrated device holds great potential for highly sensitive detection of various target analytes for medical diagnostics, food safety analysis and environmental monitoring.
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•Dialysis concentration method was firstly applied to paper-based concentration device.•Paper-based concentration device was integrated into LFAs to improve its sensitivity.•The integrated device was low-cost, simple and portable. |
| doi_str_mv | 10.1016/j.talanta.2016.02.017 |
| format | Article |
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[Display omitted]
•Dialysis concentration method was firstly applied to paper-based concentration device.•Paper-based concentration device was integrated into LFAs to improve its sensitivity.•The integrated device was low-cost, simple and portable.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2016.02.017</identifier><identifier>PMID: 26992520</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Assaying ; Biological Assay ; Devices ; Diagnostic systems ; Dialysis ; Dialysis-based concentration ; Environmental monitoring ; Foods ; Gold - chemistry ; HIV ; HIV - chemistry ; Human immunodeficiency virus ; Humans ; Lateral flow assay ; Limit of Detection ; Medical devices ; Metal Nanoparticles - chemistry ; MYO ; Myoglobin - analysis ; Paper-based device ; Point-of-Care Systems ; RNA, Viral - analysis</subject><ispartof>Talanta (Oxford), 2016-05, Vol.152, p.269-276</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-ca102d179423ac3fe4f022a517014aae0f2a8e1b3e8814aac6221c9b6ac35c393</citedby><cites>FETCH-LOGICAL-c464t-ca102d179423ac3fe4f022a517014aae0f2a8e1b3e8814aac6221c9b6ac35c393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2016.02.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26992520$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, Ruihua</creatorcontrib><creatorcontrib>Yang, Hui</creatorcontrib><creatorcontrib>Choi, Jane Ru</creatorcontrib><creatorcontrib>Gong, Yan</creatorcontrib><creatorcontrib>Hu, Jie</creatorcontrib><creatorcontrib>Feng, Shangsheng</creatorcontrib><creatorcontrib>Pingguan-Murphy, Belinda</creatorcontrib><creatorcontrib>Mei, Qibing</creatorcontrib><creatorcontrib>Xu, Feng</creatorcontrib><title>Improved sensitivity of lateral flow assay using paper-based sample concentration technique</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>Lateral flow assays (LFAs) hold great promise for point-of-care testing, especially in resource-poor settings. However, the poor sensitivity of LFAs limits their widespread applications. To address this, we developed a novel device by integrating dialysis-based concentration method into LFAs. The device successfully achieved 10-fold signal enhancement in Human Immunodeficiency Virus (HIV) nucleic acid detection with a detection limit of 0.1nM and 4-fold signal enhancement in myoglobin (MYO) detection with a detection limit of 1.56ng/mL in less than 25min. This simple, low-cost and portable integrated device holds great potential for highly sensitive detection of various target analytes for medical diagnostics, food safety analysis and environmental monitoring.
[Display omitted]
•Dialysis concentration method was firstly applied to paper-based concentration device.•Paper-based concentration device was integrated into LFAs to improve its sensitivity.•The integrated device was low-cost, simple and portable.</description><subject>Assaying</subject><subject>Biological Assay</subject><subject>Devices</subject><subject>Diagnostic systems</subject><subject>Dialysis</subject><subject>Dialysis-based concentration</subject><subject>Environmental monitoring</subject><subject>Foods</subject><subject>Gold - chemistry</subject><subject>HIV</subject><subject>HIV - chemistry</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Lateral flow assay</subject><subject>Limit of Detection</subject><subject>Medical devices</subject><subject>Metal Nanoparticles - chemistry</subject><subject>MYO</subject><subject>Myoglobin - analysis</subject><subject>Paper-based device</subject><subject>Point-of-Care Systems</subject><subject>RNA, Viral - analysis</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS1ERZfCRwD5yCVhPE7i5IRQRUulSlzoiYM160zAq_zD9i7ab49Xu3DtyRrr9-Zp3hPinYJSgWo-7spEI82JSsxjCViCMi_ERrVGF7o2-qXYAOiu6FQF1-J1jDsAQA36lbjGpuuwRtiIHw_TGpYD9zLyHH3yB5-OchnkSIkDjXIYlz-SYqSj3Ec__5QrrRyKLcWThqZ1ZOmW2fGcAiW_zDKx-zX733t-I64GGiO_vbw34unuy_fbr8Xjt_uH28-PhauaKhWOFGCvTFehJqcHrgZApFoZUBURw4DUstpqbtvTh2sQleu2TYZrpzt9Iz6c9-ZLsm1MdvLR8Zjj4WUfrWpVA6atDT6PGlPVGhGqjNZn1IUlxsCDXYOfKBytAnuqwO7spQJ7qsAC2lxB1r2_WOy3E_f_Vf8yz8CnM8A5k4PnYKPznBPsfWCXbL_4Zyz-AkohmzQ</recordid><startdate>20160515</startdate><enddate>20160515</enddate><creator>Tang, Ruihua</creator><creator>Yang, Hui</creator><creator>Choi, Jane Ru</creator><creator>Gong, Yan</creator><creator>Hu, Jie</creator><creator>Feng, Shangsheng</creator><creator>Pingguan-Murphy, Belinda</creator><creator>Mei, Qibing</creator><creator>Xu, Feng</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QQ</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20160515</creationdate><title>Improved sensitivity of lateral flow assay using paper-based sample concentration technique</title><author>Tang, Ruihua ; 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However, the poor sensitivity of LFAs limits their widespread applications. To address this, we developed a novel device by integrating dialysis-based concentration method into LFAs. The device successfully achieved 10-fold signal enhancement in Human Immunodeficiency Virus (HIV) nucleic acid detection with a detection limit of 0.1nM and 4-fold signal enhancement in myoglobin (MYO) detection with a detection limit of 1.56ng/mL in less than 25min. This simple, low-cost and portable integrated device holds great potential for highly sensitive detection of various target analytes for medical diagnostics, food safety analysis and environmental monitoring.
[Display omitted]
•Dialysis concentration method was firstly applied to paper-based concentration device.•Paper-based concentration device was integrated into LFAs to improve its sensitivity.•The integrated device was low-cost, simple and portable.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26992520</pmid><doi>10.1016/j.talanta.2016.02.017</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Assaying Biological Assay Devices Diagnostic systems Dialysis Dialysis-based concentration Environmental monitoring Foods Gold - chemistry HIV HIV - chemistry Human immunodeficiency virus Humans Lateral flow assay Limit of Detection Medical devices Metal Nanoparticles - chemistry MYO Myoglobin - analysis Paper-based device Point-of-Care Systems RNA, Viral - analysis |
| title | Improved sensitivity of lateral flow assay using paper-based sample concentration technique |
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