A non-derivative method for the quantitative analysis of isosteroidal alkaloids from Fritillaria by high performance liquid chromatography combined with charged aerosol detection
A non-derivative method was developed for the qualitative and quantitative analysis of isosteroidal alkaloids from Fritillaria thunbergii. During method development the performance of two universal detectors, the charged aerosol detector (CAD) and evaporative light scattering detector (ELSD), were e...
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| Veröffentlicht in: | Talanta (Oxford) 2016-05, Vol.151, p.239-244 |
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| creator | Long, Zhen Guo, Zhimou Acworth, Ian N. Liu, Xiaoda Jin, Yan Liu, Xingguo Liu, Lvye Liang, Lina |
| description | A non-derivative method was developed for the qualitative and quantitative analysis of isosteroidal alkaloids from Fritillaria thunbergii. During method development the performance of two universal detectors, the charged aerosol detector (CAD) and evaporative light scattering detector (ELSD), were evaluated. The CAD was found to be 30 to 55 times more sensitive than ELSD enabling the measurement of low levels of reference compound impurities that could not be detected by ELSD. The peak area percent of the reference compound, peimisine, obtained by CAD was 50.10%, but 91.66% by ELSD showing that CAD is suitable to estimate the presence of impurities. The CAD showed good reproducibility with overall intra- and inter-day peak area RSD values of less than 1.8% and 2.7%, respectively and had a linear dynamic range of up to 4 orders of magnitude (0.06–44mg/L) for peimine and peiminine. The optimized method was used for the quantitative analysis of peimine and peiminine from F. thunbergii.
A non-derivative method for the quantitative analysis of isosteroidal alkaloids from Fritillaria by high performance liquid chromatography combined with charged aerosol detection. The current method is more sensitive (LOQ |
| doi_str_mv | 10.1016/j.talanta.2016.01.027 |
| format | Article |
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A non-derivative method for the quantitative analysis of isosteroidal alkaloids from Fritillaria by high performance liquid chromatography combined with charged aerosol detection. The current method is more sensitive (LOQ<0.09ppm) and more robust (RSD<2.7%) than reported ELSD method.
[Display omitted]
•A non-derivatization method was optimized for isosteroidal alkaloids.•A Sensitive method for isosteroidal alkaloids (LOQ A method with good intra-day RSD).•The performance of CAD and ELSD were systematically compared.•Isosteroidal alkaloid impurities analyzed, and peimine and peiminine determined.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2016.01.027</identifier><identifier>PMID: 26946033</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aerosols ; Aerosols - analysis ; Alkaloids ; Alkaloids - analysis ; Cevanes - analysis ; Charged aerosol detector ; Charging ; Chromatography, High Pressure Liquid - methods ; Detectors ; Evaporative ; Evaporative light scattering detector ; Fritillaria - chemistry ; Impurities ; Isosteroidal alkaloids ; Low level ; Quantitative ; Quantitative analysis ; Reproducibility of Results ; Weak UV absorbance compound</subject><ispartof>Talanta (Oxford), 2016-05, Vol.151, p.239-244</ispartof><rights>2016</rights><rights>Copyright © 2016. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-2c82cda344b62a45aca62f99dce2ba53a68d4b2655863e38fa979b607e63411e3</citedby><cites>FETCH-LOGICAL-c398t-2c82cda344b62a45aca62f99dce2ba53a68d4b2655863e38fa979b607e63411e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2016.01.027$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26946033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Long, Zhen</creatorcontrib><creatorcontrib>Guo, Zhimou</creatorcontrib><creatorcontrib>Acworth, Ian N.</creatorcontrib><creatorcontrib>Liu, Xiaoda</creatorcontrib><creatorcontrib>Jin, Yan</creatorcontrib><creatorcontrib>Liu, Xingguo</creatorcontrib><creatorcontrib>Liu, Lvye</creatorcontrib><creatorcontrib>Liang, Lina</creatorcontrib><title>A non-derivative method for the quantitative analysis of isosteroidal alkaloids from Fritillaria by high performance liquid chromatography combined with charged aerosol detection</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>A non-derivative method was developed for the qualitative and quantitative analysis of isosteroidal alkaloids from Fritillaria thunbergii. During method development the performance of two universal detectors, the charged aerosol detector (CAD) and evaporative light scattering detector (ELSD), were evaluated. The CAD was found to be 30 to 55 times more sensitive than ELSD enabling the measurement of low levels of reference compound impurities that could not be detected by ELSD. The peak area percent of the reference compound, peimisine, obtained by CAD was 50.10%, but 91.66% by ELSD showing that CAD is suitable to estimate the presence of impurities. The CAD showed good reproducibility with overall intra- and inter-day peak area RSD values of less than 1.8% and 2.7%, respectively and had a linear dynamic range of up to 4 orders of magnitude (0.06–44mg/L) for peimine and peiminine. The optimized method was used for the quantitative analysis of peimine and peiminine from F. thunbergii.
A non-derivative method for the quantitative analysis of isosteroidal alkaloids from Fritillaria by high performance liquid chromatography combined with charged aerosol detection. The current method is more sensitive (LOQ<0.09ppm) and more robust (RSD<2.7%) than reported ELSD method.
[Display omitted]
•A non-derivatization method was optimized for isosteroidal alkaloids.•A Sensitive method for isosteroidal alkaloids (LOQ A method with good intra-day RSD).•The performance of CAD and ELSD were systematically compared.•Isosteroidal alkaloid impurities analyzed, and peimine and peiminine determined.</description><subject>Aerosols</subject><subject>Aerosols - analysis</subject><subject>Alkaloids</subject><subject>Alkaloids - analysis</subject><subject>Cevanes - analysis</subject><subject>Charged aerosol detector</subject><subject>Charging</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Detectors</subject><subject>Evaporative</subject><subject>Evaporative light scattering detector</subject><subject>Fritillaria - chemistry</subject><subject>Impurities</subject><subject>Isosteroidal alkaloids</subject><subject>Low level</subject><subject>Quantitative</subject><subject>Quantitative analysis</subject><subject>Reproducibility of Results</subject><subject>Weak UV absorbance compound</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEotvCTwD5yCXBH_k8oaqiFKkSFzhbE3uymSWJd21nq_1b_EJc7cK1J3s0z8yred8s-yB4IbioP--KCBMsEQqZyoKLgsvmVbYRbaNyVTXqdbbhXHV5J0p-lV2HsOOcS8XV2-xK1l1Zc6U22Z9btrglt-jpCJGOyGaMo7NscJ7FEdlhTSIUzz1YYDoFCswNjIILEb0jCxOD6TdM6RvY4N3M7j1FmibwBKw_sZG2I9ujTztnWAyyiQ4rWWbGBEN0Ww_78cSMm3ta0LInimNqgt-mApJGcBOzGNFEcsu77M0AU8D3l_cm-3X_9efdQ_7449v3u9vH3Kiujbk0rTQWVFn2tYSyAgO1HLrOGpQ9VArq1pa9rKuqrRWqdoCu6fqaN1irUghUN9mn8969d4cVQ9QzBYPprAXdGrRoRaKlEu3LaNOIsuKyEgmtzqhJZwWPg957msGftOD6OVm905dk9XOymgudkk1zHy8Saz-j_T_1L8oEfDkDmDw5EnodDGFy25JPxmnr6AWJv_jhvFA</recordid><startdate>20160501</startdate><enddate>20160501</enddate><creator>Long, Zhen</creator><creator>Guo, Zhimou</creator><creator>Acworth, Ian N.</creator><creator>Liu, Xiaoda</creator><creator>Jin, Yan</creator><creator>Liu, Xingguo</creator><creator>Liu, Lvye</creator><creator>Liang, Lina</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QQ</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20160501</creationdate><title>A non-derivative method for the quantitative analysis of isosteroidal alkaloids from Fritillaria by high performance liquid chromatography combined with charged aerosol detection</title><author>Long, Zhen ; Guo, Zhimou ; Acworth, Ian N. ; Liu, Xiaoda ; Jin, Yan ; Liu, Xingguo ; Liu, Lvye ; Liang, Lina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-2c82cda344b62a45aca62f99dce2ba53a68d4b2655863e38fa979b607e63411e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aerosols</topic><topic>Aerosols - analysis</topic><topic>Alkaloids</topic><topic>Alkaloids - analysis</topic><topic>Cevanes - analysis</topic><topic>Charged aerosol detector</topic><topic>Charging</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Detectors</topic><topic>Evaporative</topic><topic>Evaporative light scattering detector</topic><topic>Fritillaria - chemistry</topic><topic>Impurities</topic><topic>Isosteroidal alkaloids</topic><topic>Low level</topic><topic>Quantitative</topic><topic>Quantitative analysis</topic><topic>Reproducibility of Results</topic><topic>Weak UV absorbance compound</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Long, Zhen</creatorcontrib><creatorcontrib>Guo, Zhimou</creatorcontrib><creatorcontrib>Acworth, Ian N.</creatorcontrib><creatorcontrib>Liu, Xiaoda</creatorcontrib><creatorcontrib>Jin, Yan</creatorcontrib><creatorcontrib>Liu, Xingguo</creatorcontrib><creatorcontrib>Liu, Lvye</creatorcontrib><creatorcontrib>Liang, Lina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Ceramic Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Long, Zhen</au><au>Guo, Zhimou</au><au>Acworth, Ian N.</au><au>Liu, Xiaoda</au><au>Jin, Yan</au><au>Liu, Xingguo</au><au>Liu, Lvye</au><au>Liang, Lina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A non-derivative method for the quantitative analysis of isosteroidal alkaloids from Fritillaria by high performance liquid chromatography combined with charged aerosol detection</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2016-05-01</date><risdate>2016</risdate><volume>151</volume><spage>239</spage><epage>244</epage><pages>239-244</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><abstract>A non-derivative method was developed for the qualitative and quantitative analysis of isosteroidal alkaloids from Fritillaria thunbergii. During method development the performance of two universal detectors, the charged aerosol detector (CAD) and evaporative light scattering detector (ELSD), were evaluated. The CAD was found to be 30 to 55 times more sensitive than ELSD enabling the measurement of low levels of reference compound impurities that could not be detected by ELSD. The peak area percent of the reference compound, peimisine, obtained by CAD was 50.10%, but 91.66% by ELSD showing that CAD is suitable to estimate the presence of impurities. The CAD showed good reproducibility with overall intra- and inter-day peak area RSD values of less than 1.8% and 2.7%, respectively and had a linear dynamic range of up to 4 orders of magnitude (0.06–44mg/L) for peimine and peiminine. The optimized method was used for the quantitative analysis of peimine and peiminine from F. thunbergii.
A non-derivative method for the quantitative analysis of isosteroidal alkaloids from Fritillaria by high performance liquid chromatography combined with charged aerosol detection. The current method is more sensitive (LOQ<0.09ppm) and more robust (RSD<2.7%) than reported ELSD method.
[Display omitted]
•A non-derivatization method was optimized for isosteroidal alkaloids.•A Sensitive method for isosteroidal alkaloids (LOQ A method with good intra-day RSD).•The performance of CAD and ELSD were systematically compared.•Isosteroidal alkaloid impurities analyzed, and peimine and peiminine determined.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26946033</pmid><doi>10.1016/j.talanta.2016.01.027</doi><tpages>6</tpages></addata></record> |
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| ispartof | Talanta (Oxford), 2016-05, Vol.151, p.239-244 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Aerosols Aerosols - analysis Alkaloids Alkaloids - analysis Cevanes - analysis Charged aerosol detector Charging Chromatography, High Pressure Liquid - methods Detectors Evaporative Evaporative light scattering detector Fritillaria - chemistry Impurities Isosteroidal alkaloids Low level Quantitative Quantitative analysis Reproducibility of Results Weak UV absorbance compound |
| title | A non-derivative method for the quantitative analysis of isosteroidal alkaloids from Fritillaria by high performance liquid chromatography combined with charged aerosol detection |
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