Flow Confinement Enhancement of Heterogeneous Immunoassays in Microfluidics
This paper aims to enhance the analyte transport toward the sensing area and to enhance biosensors performances. A microchannel-based flow confinement method for rapid delivery of small sample volumes to sensor surface is described. To confine the flow, a sample flow is joined with a perpendicular m...
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| Veröffentlicht in: | IEEE sensors journal 2015-12, Vol.15 (12), p.7321-7328 |
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| creator | Selmi, Marwa Echouchene, Fraj Gazzah, Mohamed Hichem Belmabrouk, Hafedh |
| description | This paper aims to enhance the analyte transport toward the sensing area and to enhance biosensors performances. A microchannel-based flow confinement method for rapid delivery of small sample volumes to sensor surface is described. To confine the flow, a sample flow is joined with a perpendicular makeup flow and is confined into a thin layer above the reaction surface. The numerical simulation of the confinement effect on the binding reaction is performed using the finite-element method. The influence of several parameters on the binding reaction is provided, such as the average flow velocity at the inlet of the microchannel and the velocity of the confinement flow, the characteristics of the flow confinement. The numerical results reveal that the flow confinement enhances mass transport of analytes and increase its velocity. The obtained results could be exploited in flow injection experiments for low volume. |
| doi_str_mv | 10.1109/JSEN.2015.2475610 |
| format | Article |
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A microchannel-based flow confinement method for rapid delivery of small sample volumes to sensor surface is described. To confine the flow, a sample flow is joined with a perpendicular makeup flow and is confined into a thin layer above the reaction surface. The numerical simulation of the confinement effect on the binding reaction is performed using the finite-element method. The influence of several parameters on the binding reaction is provided, such as the average flow velocity at the inlet of the microchannel and the velocity of the confinement flow, the characteristics of the flow confinement. The numerical results reveal that the flow confinement enhances mass transport of analytes and increase its velocity. The obtained results could be exploited in flow injection experiments for low volume.</description><identifier>ISSN: 1530-437X</identifier><identifier>EISSN: 1558-1748</identifier><identifier>DOI: 10.1109/JSEN.2015.2475610</identifier><identifier>CODEN: ISJEAZ</identifier><language>eng</language><publisher>IEEE</publisher><subject>Binding ; Biosensor ; Biosensors ; Confinement ; confinement flow ; Immune system ; Immunoassay ; Mathematical analysis ; Mathematical model ; Mathematical models ; microchannel immunoassay ; Microchannels ; Microfluidics ; Numerical models ; reversible binding ; Sensors ; simulation ; Thin films ; Transport</subject><ispartof>IEEE sensors journal, 2015-12, Vol.15 (12), p.7321-7328</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c298t-aa9f36cd6e6f3497d388904ef46d64b8311b3ac815d5e41096818a05d0ced21a3</citedby><cites>FETCH-LOGICAL-c298t-aa9f36cd6e6f3497d388904ef46d64b8311b3ac815d5e41096818a05d0ced21a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/7234859$$EHTML$$P50$$Gieee$$H</linktohtml><link.rule.ids>314,780,784,796,27924,27925,54758</link.rule.ids><linktorsrc>$$Uhttps://ieeexplore.ieee.org/document/7234859$$EView_record_in_IEEE$$FView_record_in_$$GIEEE</linktorsrc></links><search><creatorcontrib>Selmi, Marwa</creatorcontrib><creatorcontrib>Echouchene, Fraj</creatorcontrib><creatorcontrib>Gazzah, Mohamed Hichem</creatorcontrib><creatorcontrib>Belmabrouk, Hafedh</creatorcontrib><title>Flow Confinement Enhancement of Heterogeneous Immunoassays in Microfluidics</title><title>IEEE sensors journal</title><addtitle>JSEN</addtitle><description>This paper aims to enhance the analyte transport toward the sensing area and to enhance biosensors performances. A microchannel-based flow confinement method for rapid delivery of small sample volumes to sensor surface is described. To confine the flow, a sample flow is joined with a perpendicular makeup flow and is confined into a thin layer above the reaction surface. The numerical simulation of the confinement effect on the binding reaction is performed using the finite-element method. The influence of several parameters on the binding reaction is provided, such as the average flow velocity at the inlet of the microchannel and the velocity of the confinement flow, the characteristics of the flow confinement. The numerical results reveal that the flow confinement enhances mass transport of analytes and increase its velocity. The obtained results could be exploited in flow injection experiments for low volume.</description><subject>Binding</subject><subject>Biosensor</subject><subject>Biosensors</subject><subject>Confinement</subject><subject>confinement flow</subject><subject>Immune system</subject><subject>Immunoassay</subject><subject>Mathematical analysis</subject><subject>Mathematical model</subject><subject>Mathematical models</subject><subject>microchannel immunoassay</subject><subject>Microchannels</subject><subject>Microfluidics</subject><subject>Numerical models</subject><subject>reversible binding</subject><subject>Sensors</subject><subject>simulation</subject><subject>Thin films</subject><subject>Transport</subject><issn>1530-437X</issn><issn>1558-1748</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>RIE</sourceid><recordid>eNo9kM1OwzAQhC0EEqXwAIhLjlxSvPFP7COqWloocAAkbpbrbCAocUqcCPXtSUjFaecwM5r9CLkEOgOg-ub-ZfE0SyiIWcJTIYEekQkIoWJIuToeNKMxZ-n7KTkL4YtS0KlIJ-RhWdY_0bz2eeGxQt9GC_9pvRt1nUcrbLGpP9Bj3YVoXVWdr20Idh-iwkePhWvqvOyKrHDhnJzktgx4cbhT8rZcvM5X8eb5bj2_3cQu0aqNrdU5ky6TKHPGdZoxpTTlmHOZSb5VDGDLrFMgMoG8f04qUJaKjDrMErBsSq7H3l1Tf3cYWlMVwWFZ2r-RBhRIKjUo3lthtPYzQ2gwN7umqGyzN0DNAM4M4MwAzhzA9ZmrMVMg4r8_TRhXQrNfxipqTw</recordid><startdate>201512</startdate><enddate>201512</enddate><creator>Selmi, Marwa</creator><creator>Echouchene, Fraj</creator><creator>Gazzah, Mohamed Hichem</creator><creator>Belmabrouk, Hafedh</creator><general>IEEE</general><scope>97E</scope><scope>RIA</scope><scope>RIE</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>L7M</scope></search><sort><creationdate>201512</creationdate><title>Flow Confinement Enhancement of Heterogeneous Immunoassays in Microfluidics</title><author>Selmi, Marwa ; Echouchene, Fraj ; Gazzah, Mohamed Hichem ; Belmabrouk, Hafedh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c298t-aa9f36cd6e6f3497d388904ef46d64b8311b3ac815d5e41096818a05d0ced21a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Binding</topic><topic>Biosensor</topic><topic>Biosensors</topic><topic>Confinement</topic><topic>confinement flow</topic><topic>Immune system</topic><topic>Immunoassay</topic><topic>Mathematical analysis</topic><topic>Mathematical model</topic><topic>Mathematical models</topic><topic>microchannel immunoassay</topic><topic>Microchannels</topic><topic>Microfluidics</topic><topic>Numerical models</topic><topic>reversible binding</topic><topic>Sensors</topic><topic>simulation</topic><topic>Thin films</topic><topic>Transport</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Selmi, Marwa</creatorcontrib><creatorcontrib>Echouchene, Fraj</creatorcontrib><creatorcontrib>Gazzah, Mohamed Hichem</creatorcontrib><creatorcontrib>Belmabrouk, Hafedh</creatorcontrib><collection>IEEE All-Society Periodicals Package (ASPP) 2005-present</collection><collection>IEEE All-Society Periodicals Package (ASPP) 1998-Present</collection><collection>IEEE Electronic Library (IEL)</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>IEEE sensors journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Selmi, Marwa</au><au>Echouchene, Fraj</au><au>Gazzah, Mohamed Hichem</au><au>Belmabrouk, Hafedh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flow Confinement Enhancement of Heterogeneous Immunoassays in Microfluidics</atitle><jtitle>IEEE sensors journal</jtitle><stitle>JSEN</stitle><date>2015-12</date><risdate>2015</risdate><volume>15</volume><issue>12</issue><spage>7321</spage><epage>7328</epage><pages>7321-7328</pages><issn>1530-437X</issn><eissn>1558-1748</eissn><coden>ISJEAZ</coden><abstract>This paper aims to enhance the analyte transport toward the sensing area and to enhance biosensors performances. A microchannel-based flow confinement method for rapid delivery of small sample volumes to sensor surface is described. To confine the flow, a sample flow is joined with a perpendicular makeup flow and is confined into a thin layer above the reaction surface. The numerical simulation of the confinement effect on the binding reaction is performed using the finite-element method. The influence of several parameters on the binding reaction is provided, such as the average flow velocity at the inlet of the microchannel and the velocity of the confinement flow, the characteristics of the flow confinement. The numerical results reveal that the flow confinement enhances mass transport of analytes and increase its velocity. The obtained results could be exploited in flow injection experiments for low volume.</abstract><pub>IEEE</pub><doi>10.1109/JSEN.2015.2475610</doi><tpages>8</tpages></addata></record> |
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| subjects | Binding Biosensor Biosensors Confinement confinement flow Immune system Immunoassay Mathematical analysis Mathematical model Mathematical models microchannel immunoassay Microchannels Microfluidics Numerical models reversible binding Sensors simulation Thin films Transport |
| title | Flow Confinement Enhancement of Heterogeneous Immunoassays in Microfluidics |
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