Ternary covalent conjugate (antibody-gold nanoparticle-peroxidase) for signal enhancement in enzyme immunoassay

A synthesis of a new horseradish peroxidase (HRP) conjugate and antibody covalently bound with gold nanoparticles (GNPs) with a high enzyme content was first developed. The synthesis conditions were optimized by varying the concentrations of the reacting compounds. In the conjugate, which was synthe...

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Veröffentlicht in:	RSC advances 2016-01, Vol.6 (54), p.48827-48833
Hauptverfasser:	Vdovenko, M. M, Byzova, N. A, Zherdev, A. V, Dzantiev, B. B, Sakharov, I. Yu
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Antibodies Assaying Chemiluminescence Conjugates Enzymes Gold Synthesis
Online-Zugang:	Volltext
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