A fluorometric assay for alkaline phosphatase activity based on β-cyclodextrin-modified carbon quantum dots through host-guest recognition
A convenient, reliable and highly sensitive assay for alkaline phosphatase (ALP) activity in the real-time manner is developed based on β-cyclodextrin-modified carbon quantum dots (β-CD-CQDs) nanoprobe through specific host-guest recognition. Carbon quantum dots were first functionalized with 3-amin...
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| Veröffentlicht in: | Biosensors & bioelectronics 2016-09, Vol.83, p.274-280 |
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| creator | Tang, Cong Qian, Zhaosheng Huang, Yuanyuan Xu, Jiamin Ao, Hang Zhao, Meizhi Zhou, Jin Chen, Jianrong Feng, Hui |
| description | A convenient, reliable and highly sensitive assay for alkaline phosphatase (ALP) activity in the real-time manner is developed based on β-cyclodextrin-modified carbon quantum dots (β-CD-CQDs) nanoprobe through specific host-guest recognition. Carbon quantum dots were first functionalized with 3-aminophenyl boronic acid to produce boronic acid-functionalized CQDs, and then further modified with hydropropyl β-cyclodextrins (β-CD) through B-O bonds to form β-CD-CQDs nanoprobe. p-Nitrophenol phosphate disodium salt is used as the substrate of ALP, and can hydrolyze to p-nitrophenol under the catalysis of ALP. The resulting p-nitrophenol can enter the cavity of β-CD moiety in the nanoprobe due to their specific host-guest recognition, where photoinduced electron transfer process between p-nitrophenol and CQDs takes place to efficiently quench the fluorescence of the probe. The correlation between quenched fluorescence and ALP level can be used to establish quantitative evaluation of ALP activity in a broad range from 3.4 to 100.0U/L with the detection limit of 0.9U/L. This assay shows a high sensitivity to ALP even in the presence of a very high concentration of glucose. This study demonstrates a good electron donor/acceptor pair, which can be used to design general detection strategy through PET process, and also broadens the application of host-guest recognition for enzymes detection in clinical practice.
•A sensitive assay for ALP activity based on β-CD-CQDs nanoprobe was developed for the first time.•A novel detection strategy based on specific host-guest recognition and PET of CQDs was identified.•This detection approach for ALP level is ultra-sensitive with the detection limit of 0.9U/L. |
| doi_str_mv | 10.1016/j.bios.2016.04.047 |
| format | Article |
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•A sensitive assay for ALP activity based on β-CD-CQDs nanoprobe was developed for the first time.•A novel detection strategy based on specific host-guest recognition and PET of CQDs was identified.•This detection approach for ALP level is ultra-sensitive with the detection limit of 0.9U/L.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2016.04.047</identifier><identifier>PMID: 27132001</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Alkaline phosphatase ; Alkaline phosphatase (ALP) activity ; Alkaline Phosphatase - analysis ; Alkaline Phosphatase - metabolism ; Animals ; Assaying ; beta-Cyclodextrins - chemistry ; beta-Cyclodextrins - metabolism ; Biosensing Techniques - methods ; Carbon ; Carbon - chemistry ; Carbon - metabolism ; Carbon quantum dots ; Cattle ; Electron transfer ; Enzyme Assays - methods ; Fluorescence ; Fluorometry - methods ; Limit of Detection ; Nanostructure ; Nitrophenols - metabolism ; Organophosphorus Compounds - metabolism ; Photoinduced electron transfer (PET) ; Quantum dots ; Quantum Dots - chemistry ; Quantum Dots - metabolism ; Quantum Dots - ultrastructure ; Real-time fluorometric assay ; Recognition</subject><ispartof>Biosensors & bioelectronics, 2016-09, Vol.83, p.274-280</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-6a606dd441e1dea7263a064701a9b086f484b533b8efbd85d5a0d645f44839233</citedby><cites>FETCH-LOGICAL-c422t-6a606dd441e1dea7263a064701a9b086f484b533b8efbd85d5a0d645f44839233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2016.04.047$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27132001$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, Cong</creatorcontrib><creatorcontrib>Qian, Zhaosheng</creatorcontrib><creatorcontrib>Huang, Yuanyuan</creatorcontrib><creatorcontrib>Xu, Jiamin</creatorcontrib><creatorcontrib>Ao, Hang</creatorcontrib><creatorcontrib>Zhao, Meizhi</creatorcontrib><creatorcontrib>Zhou, Jin</creatorcontrib><creatorcontrib>Chen, Jianrong</creatorcontrib><creatorcontrib>Feng, Hui</creatorcontrib><title>A fluorometric assay for alkaline phosphatase activity based on β-cyclodextrin-modified carbon quantum dots through host-guest recognition</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>A convenient, reliable and highly sensitive assay for alkaline phosphatase (ALP) activity in the real-time manner is developed based on β-cyclodextrin-modified carbon quantum dots (β-CD-CQDs) nanoprobe through specific host-guest recognition. Carbon quantum dots were first functionalized with 3-aminophenyl boronic acid to produce boronic acid-functionalized CQDs, and then further modified with hydropropyl β-cyclodextrins (β-CD) through B-O bonds to form β-CD-CQDs nanoprobe. p-Nitrophenol phosphate disodium salt is used as the substrate of ALP, and can hydrolyze to p-nitrophenol under the catalysis of ALP. The resulting p-nitrophenol can enter the cavity of β-CD moiety in the nanoprobe due to their specific host-guest recognition, where photoinduced electron transfer process between p-nitrophenol and CQDs takes place to efficiently quench the fluorescence of the probe. The correlation between quenched fluorescence and ALP level can be used to establish quantitative evaluation of ALP activity in a broad range from 3.4 to 100.0U/L with the detection limit of 0.9U/L. This assay shows a high sensitivity to ALP even in the presence of a very high concentration of glucose. This study demonstrates a good electron donor/acceptor pair, which can be used to design general detection strategy through PET process, and also broadens the application of host-guest recognition for enzymes detection in clinical practice.
•A sensitive assay for ALP activity based on β-CD-CQDs nanoprobe was developed for the first time.•A novel detection strategy based on specific host-guest recognition and PET of CQDs was identified.•This detection approach for ALP level is ultra-sensitive with the detection limit of 0.9U/L.</description><subject>Alkaline phosphatase</subject><subject>Alkaline phosphatase (ALP) activity</subject><subject>Alkaline Phosphatase - analysis</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Assaying</subject><subject>beta-Cyclodextrins - chemistry</subject><subject>beta-Cyclodextrins - metabolism</subject><subject>Biosensing Techniques - methods</subject><subject>Carbon</subject><subject>Carbon - chemistry</subject><subject>Carbon - metabolism</subject><subject>Carbon quantum dots</subject><subject>Cattle</subject><subject>Electron transfer</subject><subject>Enzyme Assays - methods</subject><subject>Fluorescence</subject><subject>Fluorometry - methods</subject><subject>Limit of Detection</subject><subject>Nanostructure</subject><subject>Nitrophenols - metabolism</subject><subject>Organophosphorus Compounds - metabolism</subject><subject>Photoinduced electron transfer (PET)</subject><subject>Quantum dots</subject><subject>Quantum Dots - chemistry</subject><subject>Quantum Dots - metabolism</subject><subject>Quantum Dots - ultrastructure</subject><subject>Real-time fluorometric assay</subject><subject>Recognition</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkd9qFDEUh4NY7Fp9AS8kl97MejLJ5A94U4pVoeBNvQ6ZJLObdWayTTKl-wy-TR_EZzLLVi9VOJCE852PcH4IvSGwJkD4-926DzGv23pfA6slnqEVkYI2rKXdc7QC1fGm45yeo5c57wBAEAUv0HkrCG0ByAr9uMTDuMQUJ19SsNjkbA54iAmb8bsZw-zxfhvzfmuKyR4bW8J9KAfc15fDccY_Hxt7sGN0_qEK5maKLgyh9qxJfe3fLWYuy4RdLBmXbYrLZoursTSbxeeCk7dxM4cS4vwKnQ1mzP7103mBvl1_vL363Nx8_fTl6vKmsaxtS8MNB-4cY8QT541oOTXAmQBiVA-SD0yyvqO0l37onexcZ8Bx1g2MSapaSi_Qu5N3n-Ld8RN6Ctn6cTSzj0vWRJJOKUUo_BsVCpQAStl_oFIxJZiSFW1PqE0x5-QHvU9hMumgCehjtHqnj9HqY7QaWC1Rh94--Zd-8u7PyO8sK_DhBPi6u_vgk842-Nl6F-qSi3Yx_M3_CwY-t9c</recordid><startdate>20160915</startdate><enddate>20160915</enddate><creator>Tang, Cong</creator><creator>Qian, Zhaosheng</creator><creator>Huang, Yuanyuan</creator><creator>Xu, Jiamin</creator><creator>Ao, Hang</creator><creator>Zhao, Meizhi</creator><creator>Zhou, Jin</creator><creator>Chen, Jianrong</creator><creator>Feng, Hui</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SP</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>20160915</creationdate><title>A fluorometric assay for alkaline phosphatase activity based on β-cyclodextrin-modified carbon quantum dots through host-guest recognition</title><author>Tang, Cong ; Qian, Zhaosheng ; Huang, Yuanyuan ; Xu, Jiamin ; Ao, Hang ; Zhao, Meizhi ; Zhou, Jin ; Chen, Jianrong ; Feng, Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-6a606dd441e1dea7263a064701a9b086f484b533b8efbd85d5a0d645f44839233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Alkaline phosphatase</topic><topic>Alkaline phosphatase (ALP) activity</topic><topic>Alkaline Phosphatase - analysis</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Assaying</topic><topic>beta-Cyclodextrins - chemistry</topic><topic>beta-Cyclodextrins - metabolism</topic><topic>Biosensing Techniques - methods</topic><topic>Carbon</topic><topic>Carbon - chemistry</topic><topic>Carbon - metabolism</topic><topic>Carbon quantum dots</topic><topic>Cattle</topic><topic>Electron transfer</topic><topic>Enzyme Assays - methods</topic><topic>Fluorescence</topic><topic>Fluorometry - methods</topic><topic>Limit of Detection</topic><topic>Nanostructure</topic><topic>Nitrophenols - metabolism</topic><topic>Organophosphorus Compounds - metabolism</topic><topic>Photoinduced electron transfer (PET)</topic><topic>Quantum dots</topic><topic>Quantum Dots - chemistry</topic><topic>Quantum Dots - metabolism</topic><topic>Quantum Dots - ultrastructure</topic><topic>Real-time fluorometric assay</topic><topic>Recognition</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Cong</creatorcontrib><creatorcontrib>Qian, Zhaosheng</creatorcontrib><creatorcontrib>Huang, Yuanyuan</creatorcontrib><creatorcontrib>Xu, Jiamin</creatorcontrib><creatorcontrib>Ao, Hang</creatorcontrib><creatorcontrib>Zhao, Meizhi</creatorcontrib><creatorcontrib>Zhou, Jin</creatorcontrib><creatorcontrib>Chen, Jianrong</creatorcontrib><creatorcontrib>Feng, Hui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Cong</au><au>Qian, Zhaosheng</au><au>Huang, Yuanyuan</au><au>Xu, Jiamin</au><au>Ao, Hang</au><au>Zhao, Meizhi</au><au>Zhou, Jin</au><au>Chen, Jianrong</au><au>Feng, Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A fluorometric assay for alkaline phosphatase activity based on β-cyclodextrin-modified carbon quantum dots through host-guest recognition</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2016-09-15</date><risdate>2016</risdate><volume>83</volume><spage>274</spage><epage>280</epage><pages>274-280</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>A convenient, reliable and highly sensitive assay for alkaline phosphatase (ALP) activity in the real-time manner is developed based on β-cyclodextrin-modified carbon quantum dots (β-CD-CQDs) nanoprobe through specific host-guest recognition. Carbon quantum dots were first functionalized with 3-aminophenyl boronic acid to produce boronic acid-functionalized CQDs, and then further modified with hydropropyl β-cyclodextrins (β-CD) through B-O bonds to form β-CD-CQDs nanoprobe. p-Nitrophenol phosphate disodium salt is used as the substrate of ALP, and can hydrolyze to p-nitrophenol under the catalysis of ALP. The resulting p-nitrophenol can enter the cavity of β-CD moiety in the nanoprobe due to their specific host-guest recognition, where photoinduced electron transfer process between p-nitrophenol and CQDs takes place to efficiently quench the fluorescence of the probe. The correlation between quenched fluorescence and ALP level can be used to establish quantitative evaluation of ALP activity in a broad range from 3.4 to 100.0U/L with the detection limit of 0.9U/L. This assay shows a high sensitivity to ALP even in the presence of a very high concentration of glucose. This study demonstrates a good electron donor/acceptor pair, which can be used to design general detection strategy through PET process, and also broadens the application of host-guest recognition for enzymes detection in clinical practice.
•A sensitive assay for ALP activity based on β-CD-CQDs nanoprobe was developed for the first time.•A novel detection strategy based on specific host-guest recognition and PET of CQDs was identified.•This detection approach for ALP level is ultra-sensitive with the detection limit of 0.9U/L.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>27132001</pmid><doi>10.1016/j.bios.2016.04.047</doi><tpages>7</tpages></addata></record> |
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| subjects | Alkaline phosphatase Alkaline phosphatase (ALP) activity Alkaline Phosphatase - analysis Alkaline Phosphatase - metabolism Animals Assaying beta-Cyclodextrins - chemistry beta-Cyclodextrins - metabolism Biosensing Techniques - methods Carbon Carbon - chemistry Carbon - metabolism Carbon quantum dots Cattle Electron transfer Enzyme Assays - methods Fluorescence Fluorometry - methods Limit of Detection Nanostructure Nitrophenols - metabolism Organophosphorus Compounds - metabolism Photoinduced electron transfer (PET) Quantum dots Quantum Dots - chemistry Quantum Dots - metabolism Quantum Dots - ultrastructure Real-time fluorometric assay Recognition |
| title | A fluorometric assay for alkaline phosphatase activity based on β-cyclodextrin-modified carbon quantum dots through host-guest recognition |
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