First evidence of polar flagella in Klebsiella pneumoniae isolated from a patient with neonatal sepsis
The genus Klebsiella belongs to the family Enterobacteriaceae, and is currently considered to be non-motile and non-flagellated. In the present work, 25 Klebsiella strains isolated from nosocomial infections were assessed for motility under different growth conditions. One Klebsiella isolate, KpBUAP...
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| Veröffentlicht in: | Journal of medical microbiology 2016-08, Vol.65 (8), p.729-737 |
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| creator | Carabarin-Lima, Alejandro León-Izurieta, Libia Rocha-Gracia, Rosa Del Carmen Castañeda-Lucio, Miguel Torres, Carmen Gutiérrez-Cazarez, Zita González-Posos, Sirenia Martínez de la Peña, Claudia F Martinez-Laguna, Ygnacio Lozano-Zarain, Patricia |
| description | The genus Klebsiella belongs to the family Enterobacteriaceae, and is currently considered to be non-motile and non-flagellated. In the present work, 25 Klebsiella strains isolated from nosocomial infections were assessed for motility under different growth conditions. One Klebsiella isolate, KpBUAP021, demonstrated a swim-like motility phenotype. The K. pneumoniae genotype was confirmed by 16S rRNA and rpoB gene sequence analysis. Multilocus sequence typing analysis also revealed that the KpBUAP021 strain places it in the ST345 sequence type, and belongs to the phylogenetic Kpl group. Transmission electron microscopy and the Ryu staining technique revealed that KpBUAP021 expresses polar flagella. Finally, the presence of fliC, fliA and flgH genes in this K. pneumoniae strain was confirmed. This report presents the first evidence for flagella-mediated motility in a K. pneumoniae clinical isolate, and represents an important finding related to its evolution and pathogenic potential. |
| doi_str_mv | 10.1099/jmm.0.000291 |
| format | Article |
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In the present work, 25 Klebsiella strains isolated from nosocomial infections were assessed for motility under different growth conditions. One Klebsiella isolate, KpBUAP021, demonstrated a swim-like motility phenotype. The K. pneumoniae genotype was confirmed by 16S rRNA and rpoB gene sequence analysis. Multilocus sequence typing analysis also revealed that the KpBUAP021 strain places it in the ST345 sequence type, and belongs to the phylogenetic Kpl group. Transmission electron microscopy and the Ryu staining technique revealed that KpBUAP021 expresses polar flagella. Finally, the presence of fliC, fliA and flgH genes in this K. pneumoniae strain was confirmed. 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In the present work, 25 Klebsiella strains isolated from nosocomial infections were assessed for motility under different growth conditions. One Klebsiella isolate, KpBUAP021, demonstrated a swim-like motility phenotype. The K. pneumoniae genotype was confirmed by 16S rRNA and rpoB gene sequence analysis. Multilocus sequence typing analysis also revealed that the KpBUAP021 strain places it in the ST345 sequence type, and belongs to the phylogenetic Kpl group. Transmission electron microscopy and the Ryu staining technique revealed that KpBUAP021 expresses polar flagella. Finally, the presence of fliC, fliA and flgH genes in this K. pneumoniae strain was confirmed. This report presents the first evidence for flagella-mediated motility in a K. pneumoniae clinical isolate, and represents an important finding related to its evolution and pathogenic potential.</description><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>Flagella - ultrastructure</subject><subject>Flagellin - genetics</subject><subject>Humans</subject><subject>Klebsiella Infections - microbiology</subject><subject>Klebsiella pneumoniae - cytology</subject><subject>Klebsiella pneumoniae - genetics</subject><subject>Klebsiella pneumoniae - isolation & purification</subject><subject>Klebsiella pneumoniae - physiology</subject><subject>Locomotion</subject><subject>Microscopy, Electron, Transmission</subject><subject>Multilocus Sequence Typing</subject><subject>Neonatal Sepsis - microbiology</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sigma Factor - genetics</subject><subject>Staining and Labeling</subject><issn>0022-2615</issn><issn>1473-5644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kD1PwzAQhi0EoqWwMSOPDKTYcZzEI6ooICqxwGxdnDO4yhd2AuLfY2hhOr13j16dHkLOOVtyptT1tm2XbMkYSxU_IHOeFSKReZYdknncpUmaczkjJyFsGeOFEOqYzNIiLQVX2ZzYtfNhpPjhauwM0t7SoW_AU9vAKzYNUNfRxwar4H7T0OHU9p0DpC5EcMSaWt-3NJ5gdNiN9NONb7TDvoMRGhpwCC6ckiMLTcCz_VyQl_Xt8-o-2TzdPaxuNokRqRoTKcrCAipW5JJzMLaUlZUSTclNiUKZGDGtGAOE3NYZRN4AliyDQighxIJc7noH379PGEbdumB-Po8PTUHzkktVcCFlRK92qPF9CB6tHrxrwX9pzvSPWR3NaqZ3ZiN-sW-eqhbrf_hPpfgGD_11xQ</recordid><startdate>201608</startdate><enddate>201608</enddate><creator>Carabarin-Lima, Alejandro</creator><creator>León-Izurieta, Libia</creator><creator>Rocha-Gracia, Rosa Del Carmen</creator><creator>Castañeda-Lucio, Miguel</creator><creator>Torres, Carmen</creator><creator>Gutiérrez-Cazarez, Zita</creator><creator>González-Posos, Sirenia</creator><creator>Martínez de la Peña, Claudia F</creator><creator>Martinez-Laguna, Ygnacio</creator><creator>Lozano-Zarain, Patricia</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201608</creationdate><title>First evidence of polar flagella in Klebsiella pneumoniae isolated from a patient with neonatal sepsis</title><author>Carabarin-Lima, Alejandro ; León-Izurieta, Libia ; Rocha-Gracia, Rosa Del Carmen ; Castañeda-Lucio, Miguel ; Torres, Carmen ; Gutiérrez-Cazarez, Zita ; González-Posos, Sirenia ; Martínez de la Peña, Claudia F ; Martinez-Laguna, Ygnacio ; Lozano-Zarain, Patricia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-5387fae9076511acf85bf55ec81c8e39c5bfe2b00aea6fd4a387cae804a739333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bacterial Outer Membrane Proteins - genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>DNA-Directed RNA Polymerases - genetics</topic><topic>Flagella - ultrastructure</topic><topic>Flagellin - genetics</topic><topic>Humans</topic><topic>Klebsiella Infections - microbiology</topic><topic>Klebsiella pneumoniae - cytology</topic><topic>Klebsiella pneumoniae - genetics</topic><topic>Klebsiella pneumoniae - isolation & purification</topic><topic>Klebsiella pneumoniae - physiology</topic><topic>Locomotion</topic><topic>Microscopy, Electron, Transmission</topic><topic>Multilocus Sequence Typing</topic><topic>Neonatal Sepsis - microbiology</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sigma Factor - genetics</topic><topic>Staining and Labeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carabarin-Lima, Alejandro</creatorcontrib><creatorcontrib>León-Izurieta, Libia</creatorcontrib><creatorcontrib>Rocha-Gracia, Rosa Del Carmen</creatorcontrib><creatorcontrib>Castañeda-Lucio, Miguel</creatorcontrib><creatorcontrib>Torres, Carmen</creatorcontrib><creatorcontrib>Gutiérrez-Cazarez, Zita</creatorcontrib><creatorcontrib>González-Posos, Sirenia</creatorcontrib><creatorcontrib>Martínez de la Peña, Claudia F</creatorcontrib><creatorcontrib>Martinez-Laguna, Ygnacio</creatorcontrib><creatorcontrib>Lozano-Zarain, Patricia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carabarin-Lima, Alejandro</au><au>León-Izurieta, Libia</au><au>Rocha-Gracia, Rosa Del Carmen</au><au>Castañeda-Lucio, Miguel</au><au>Torres, Carmen</au><au>Gutiérrez-Cazarez, Zita</au><au>González-Posos, Sirenia</au><au>Martínez de la Peña, Claudia F</au><au>Martinez-Laguna, Ygnacio</au><au>Lozano-Zarain, Patricia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First evidence of polar flagella in Klebsiella pneumoniae isolated from a patient with neonatal sepsis</atitle><jtitle>Journal of medical microbiology</jtitle><addtitle>J Med Microbiol</addtitle><date>2016-08</date><risdate>2016</risdate><volume>65</volume><issue>8</issue><spage>729</spage><epage>737</epage><pages>729-737</pages><issn>0022-2615</issn><eissn>1473-5644</eissn><abstract>The genus Klebsiella belongs to the family Enterobacteriaceae, and is currently considered to be non-motile and non-flagellated. In the present work, 25 Klebsiella strains isolated from nosocomial infections were assessed for motility under different growth conditions. One Klebsiella isolate, KpBUAP021, demonstrated a swim-like motility phenotype. The K. pneumoniae genotype was confirmed by 16S rRNA and rpoB gene sequence analysis. Multilocus sequence typing analysis also revealed that the KpBUAP021 strain places it in the ST345 sequence type, and belongs to the phylogenetic Kpl group. Transmission electron microscopy and the Ryu staining technique revealed that KpBUAP021 expresses polar flagella. Finally, the presence of fliC, fliA and flgH genes in this K. pneumoniae strain was confirmed. 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| subjects | Bacterial Outer Membrane Proteins - genetics Bacterial Proteins - genetics Child Child, Preschool DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics DNA-Directed RNA Polymerases - genetics Flagella - ultrastructure Flagellin - genetics Humans Klebsiella Infections - microbiology Klebsiella pneumoniae - cytology Klebsiella pneumoniae - genetics Klebsiella pneumoniae - isolation & purification Klebsiella pneumoniae - physiology Locomotion Microscopy, Electron, Transmission Multilocus Sequence Typing Neonatal Sepsis - microbiology Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Sigma Factor - genetics Staining and Labeling |
| title | First evidence of polar flagella in Klebsiella pneumoniae isolated from a patient with neonatal sepsis |
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