Gray level co-occurrence matrix algorithm as pattern recognition biosensor for oxidopamine-induced changes in lymphocyte chromatin architecture
We demonstrate that a proapoptotic chemical agent, oxidopamine, induces dose dependent changes in chromatin textural patterns which can be quantified using the Gray level co-occurrence matrix (GLCM) method. Peripheral blood (heparin-pretreated) samples were treated with oxidopamine (6-OHDA, 6-hydrox...
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| Veröffentlicht in: | Journal of theoretical biology 2016-10, Vol.406, p.124-128 |
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| creator | Pantic, Igor Dimitrijevic, Draga Nesic, Dejan Petrovic, Danica |
| description | We demonstrate that a proapoptotic chemical agent, oxidopamine, induces dose dependent changes in chromatin textural patterns which can be quantified using the Gray level co-occurrence matrix (GLCM) method. Peripheral blood (heparin-pretreated) samples were treated with oxidopamine (6-OHDA, 6-hydroxydopamine) to achieve effective concentrations of 100, 200 and 300µM. The samples were smeared on microscope slides and fixated in methanol. The smears were stained using a modification of Feulgen method for DNA visualization. For each stained smear, a sample of 30 lymphocyte chromatin structures were visualized and analyzed. This way, textural parameters for a total of 120 nuclei micrographs were calculated. For each chromatin structure, five different GLCM features were calculated: angular second moment, GLCM entropy, inverse difference moment, GLCM correlation, and GLCM variance. Oxidopamine induced the rise of the values of GLCM entropy and variance, and the reduction of angular second moment, correlation, and inverse difference moment. The trends for GLCM parameter changes were found to be highly significant (p |
| doi_str_mv | 10.1016/j.jtbi.2016.07.018 |
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•Peripheral blood was treated with 6-hydroxydopamine.•The smears were stained using a modification of Feulgen method for DNA visualization.•120 chromatin structures were analyzed GLCM mathematical algorithm.•6-hydroxydopamine induced the rise of the values of chromatin GLCM entropy and variance.</description><identifier>ISSN: 0022-5193</identifier><identifier>EISSN: 1095-8541</identifier><identifier>DOI: 10.1016/j.jtbi.2016.07.018</identifier><identifier>PMID: 27424557</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Algorithms ; Biosensing Techniques - methods ; Blood ; Chromatin - chemistry ; Dopamine ; Entropy ; Homogeneity ; Humans ; Image analysis ; Lymphocytes - drug effects ; Lymphocytes - metabolism ; Oxidopamine - pharmacology ; Pattern ; Pattern Recognition, Automated - methods ; ROC Curve</subject><ispartof>Journal of theoretical biology, 2016-10, Vol.406, p.124-128</ispartof><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-d7aadca1a869ef0f7c67a9822a82abb7f3df4aded7cfa1976298b07e077ef3293</citedby><cites>FETCH-LOGICAL-c389t-d7aadca1a869ef0f7c67a9822a82abb7f3df4aded7cfa1976298b07e077ef3293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jtbi.2016.07.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27424557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pantic, Igor</creatorcontrib><creatorcontrib>Dimitrijevic, Draga</creatorcontrib><creatorcontrib>Nesic, Dejan</creatorcontrib><creatorcontrib>Petrovic, Danica</creatorcontrib><title>Gray level co-occurrence matrix algorithm as pattern recognition biosensor for oxidopamine-induced changes in lymphocyte chromatin architecture</title><title>Journal of theoretical biology</title><addtitle>J Theor Biol</addtitle><description>We demonstrate that a proapoptotic chemical agent, oxidopamine, induces dose dependent changes in chromatin textural patterns which can be quantified using the Gray level co-occurrence matrix (GLCM) method. Peripheral blood (heparin-pretreated) samples were treated with oxidopamine (6-OHDA, 6-hydroxydopamine) to achieve effective concentrations of 100, 200 and 300µM. The samples were smeared on microscope slides and fixated in methanol. The smears were stained using a modification of Feulgen method for DNA visualization. For each stained smear, a sample of 30 lymphocyte chromatin structures were visualized and analyzed. This way, textural parameters for a total of 120 nuclei micrographs were calculated. For each chromatin structure, five different GLCM features were calculated: angular second moment, GLCM entropy, inverse difference moment, GLCM correlation, and GLCM variance. Oxidopamine induced the rise of the values of GLCM entropy and variance, and the reduction of angular second moment, correlation, and inverse difference moment. The trends for GLCM parameter changes were found to be highly significant (p<0.001). These results indicate that GLCM mathematical algorithm might be successfully used in detection and evaluation of discrete early apoptotic structural changes in Feulgen-stained chromatin of peripheral blood lymphocytes that are not detectable using conventional microscopy/cell biology techniques.
•Peripheral blood was treated with 6-hydroxydopamine.•The smears were stained using a modification of Feulgen method for DNA visualization.•120 chromatin structures were analyzed GLCM mathematical algorithm.•6-hydroxydopamine induced the rise of the values of chromatin GLCM entropy and variance.</description><subject>Algorithms</subject><subject>Biosensing Techniques - methods</subject><subject>Blood</subject><subject>Chromatin - chemistry</subject><subject>Dopamine</subject><subject>Entropy</subject><subject>Homogeneity</subject><subject>Humans</subject><subject>Image analysis</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - metabolism</subject><subject>Oxidopamine - pharmacology</subject><subject>Pattern</subject><subject>Pattern Recognition, Automated - methods</subject><subject>ROC Curve</subject><issn>0022-5193</issn><issn>1095-8541</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-KFDEQh4Mo7rj6Ah4kRy_dJuk_SYMXWdZVWPCi51CdVM9k6E7aJL3sPIWvbIZZPYqHIkXx1S9QHyFvOas54_2HY33Mo6tF6Wsma8bVM7LjbOgq1bX8OdkxJkTV8aG5Iq9SOjLGhrbpX5IrIVvRdp3ckV93EU50xgecqQlVMGaLEb1BukCO7pHCvA_R5cNCIdEVcsboaUQT9t5lFzwdXUjoU4h0KhUenQ0rLM5j5bzdDFpqDuD3mKjzdD4t6yGYU8YyjaH8UYYQzcFlNHmL-Jq8mGBO-ObpvSY_Pt9-v_lS3X-7-3rz6b4yjRpyZSWANcBB9QNObJKmlzAoIUAJGEc5NXZqwaKVZgI-yF4MamQSmZQ4NWJorsn7S-4aw88NU9aLSwbnGTyGLWmueCc5H5j6H5Srtpy0Lai4oCaGlCJOeo1ugXjSnOmzM33UZ2f67EwzqYuzsvTuKX8bF7R_V_5IKsDHC4DlIA8Oo07GnR1ZV0RkbYP7V_5vbpus_A</recordid><startdate>20161007</startdate><enddate>20161007</enddate><creator>Pantic, Igor</creator><creator>Dimitrijevic, Draga</creator><creator>Nesic, Dejan</creator><creator>Petrovic, Danica</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20161007</creationdate><title>Gray level co-occurrence matrix algorithm as pattern recognition biosensor for oxidopamine-induced changes in lymphocyte chromatin architecture</title><author>Pantic, Igor ; Dimitrijevic, Draga ; Nesic, Dejan ; Petrovic, Danica</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-d7aadca1a869ef0f7c67a9822a82abb7f3df4aded7cfa1976298b07e077ef3293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Algorithms</topic><topic>Biosensing Techniques - methods</topic><topic>Blood</topic><topic>Chromatin - chemistry</topic><topic>Dopamine</topic><topic>Entropy</topic><topic>Homogeneity</topic><topic>Humans</topic><topic>Image analysis</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - metabolism</topic><topic>Oxidopamine - pharmacology</topic><topic>Pattern</topic><topic>Pattern Recognition, Automated - methods</topic><topic>ROC Curve</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pantic, Igor</creatorcontrib><creatorcontrib>Dimitrijevic, Draga</creatorcontrib><creatorcontrib>Nesic, Dejan</creatorcontrib><creatorcontrib>Petrovic, Danica</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of theoretical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pantic, Igor</au><au>Dimitrijevic, Draga</au><au>Nesic, Dejan</au><au>Petrovic, Danica</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gray level co-occurrence matrix algorithm as pattern recognition biosensor for oxidopamine-induced changes in lymphocyte chromatin architecture</atitle><jtitle>Journal of theoretical biology</jtitle><addtitle>J Theor Biol</addtitle><date>2016-10-07</date><risdate>2016</risdate><volume>406</volume><spage>124</spage><epage>128</epage><pages>124-128</pages><issn>0022-5193</issn><eissn>1095-8541</eissn><abstract>We demonstrate that a proapoptotic chemical agent, oxidopamine, induces dose dependent changes in chromatin textural patterns which can be quantified using the Gray level co-occurrence matrix (GLCM) method. Peripheral blood (heparin-pretreated) samples were treated with oxidopamine (6-OHDA, 6-hydroxydopamine) to achieve effective concentrations of 100, 200 and 300µM. The samples were smeared on microscope slides and fixated in methanol. The smears were stained using a modification of Feulgen method for DNA visualization. For each stained smear, a sample of 30 lymphocyte chromatin structures were visualized and analyzed. This way, textural parameters for a total of 120 nuclei micrographs were calculated. For each chromatin structure, five different GLCM features were calculated: angular second moment, GLCM entropy, inverse difference moment, GLCM correlation, and GLCM variance. Oxidopamine induced the rise of the values of GLCM entropy and variance, and the reduction of angular second moment, correlation, and inverse difference moment. The trends for GLCM parameter changes were found to be highly significant (p<0.001). These results indicate that GLCM mathematical algorithm might be successfully used in detection and evaluation of discrete early apoptotic structural changes in Feulgen-stained chromatin of peripheral blood lymphocytes that are not detectable using conventional microscopy/cell biology techniques.
•Peripheral blood was treated with 6-hydroxydopamine.•The smears were stained using a modification of Feulgen method for DNA visualization.•120 chromatin structures were analyzed GLCM mathematical algorithm.•6-hydroxydopamine induced the rise of the values of chromatin GLCM entropy and variance.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>27424557</pmid><doi>10.1016/j.jtbi.2016.07.018</doi><tpages>5</tpages></addata></record> |
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| subjects | Algorithms Biosensing Techniques - methods Blood Chromatin - chemistry Dopamine Entropy Homogeneity Humans Image analysis Lymphocytes - drug effects Lymphocytes - metabolism Oxidopamine - pharmacology Pattern Pattern Recognition, Automated - methods ROC Curve |
| title | Gray level co-occurrence matrix algorithm as pattern recognition biosensor for oxidopamine-induced changes in lymphocyte chromatin architecture |
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