Applying CRISPR–Cas9 tools to identify and characterize transcriptional enhancers
CRISPR–Cas9-based genome editing tools have been developed recently to study non-coding transcriptional regulatory elements, enabling the characterization of enhancers in their endogenous context. The applications, current limitations and future development of such CRISPR–Cas9 tools are discussed, w...
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| Veröffentlicht in: | Nature reviews. Molecular cell biology 2016-09, Vol.17 (9), p.597-604 |
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| container_title | Nature reviews. Molecular cell biology |
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| creator | Lopes, Rui Korkmaz, Gozde Agami, Reuven |
| description | CRISPR–Cas9-based genome editing tools have been developed recently to study non-coding transcriptional regulatory elements, enabling the characterization of enhancers in their endogenous context. The applications, current limitations and future development of such CRISPR–Cas9 tools are discussed, with emphasis on identifying and characterizing enhancer elements in a high-throughput manner.
The development of the CRISPR–Cas9 system triggered a revolution in the field of genome engineering. Initially, the use of this system was focused on the study of protein-coding genes but, recently, a number of CRISPR–Cas9-based tools have been developed to study non-coding transcriptional regulatory elements. These technological advances offer unprecedented opportunities for elucidating the functions of enhancers in their endogenous context. Here, we discuss the application, current limitations and future development of CRISPR–Cas9 systems to identify and characterize enhancer elements in a high-throughput manner. |
| doi_str_mv | 10.1038/nrm.2016.79 |
| format | Article |
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The development of the CRISPR–Cas9 system triggered a revolution in the field of genome engineering. Initially, the use of this system was focused on the study of protein-coding genes but, recently, a number of CRISPR–Cas9-based tools have been developed to study non-coding transcriptional regulatory elements. These technological advances offer unprecedented opportunities for elucidating the functions of enhancers in their endogenous context. Here, we discuss the application, current limitations and future development of CRISPR–Cas9 systems to identify and characterize enhancer elements in a high-throughput manner.</description><identifier>ISSN: 1471-0072</identifier><identifier>EISSN: 1471-0080</identifier><identifier>DOI: 10.1038/nrm.2016.79</identifier><identifier>PMID: 27381243</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647/2163 ; 631/337/4041/3196 ; 631/337/572/2102 ; Biochemistry ; Cancer Research ; Cell Biology ; CRISPR ; CRISPR-Cas Systems ; Developmental Biology ; Enhancer Elements, Genetic ; Epigenesis, Genetic ; Epigenetics ; Epigenomics - methods ; Gene Editing - methods ; Gene expression ; Genetic engineering ; Genetic research ; Genetic transcription ; Genomes ; Hemoglobin ; innovation ; Innovations ; Life Sciences ; Proteins ; RNA polymerase ; Stem Cells ; Transcription factors</subject><ispartof>Nature reviews. Molecular cell biology, 2016-09, Vol.17 (9), p.597-604</ispartof><rights>Springer Nature Limited 2016</rights><rights>COPYRIGHT 2016 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Sep 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-800fb7426a14ae643f851fd436978ad7bede1949c3d2a049ca28ce5c4cf6cb413</citedby><cites>FETCH-LOGICAL-c554t-800fb7426a14ae643f851fd436978ad7bede1949c3d2a049ca28ce5c4cf6cb413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nrm.2016.79$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nrm.2016.79$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27381243$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lopes, Rui</creatorcontrib><creatorcontrib>Korkmaz, Gozde</creatorcontrib><creatorcontrib>Agami, Reuven</creatorcontrib><title>Applying CRISPR–Cas9 tools to identify and characterize transcriptional enhancers</title><title>Nature reviews. Molecular cell biology</title><addtitle>Nat Rev Mol Cell Biol</addtitle><addtitle>Nat Rev Mol Cell Biol</addtitle><description>CRISPR–Cas9-based genome editing tools have been developed recently to study non-coding transcriptional regulatory elements, enabling the characterization of enhancers in their endogenous context. The applications, current limitations and future development of such CRISPR–Cas9 tools are discussed, with emphasis on identifying and characterizing enhancer elements in a high-throughput manner.
The development of the CRISPR–Cas9 system triggered a revolution in the field of genome engineering. Initially, the use of this system was focused on the study of protein-coding genes but, recently, a number of CRISPR–Cas9-based tools have been developed to study non-coding transcriptional regulatory elements. These technological advances offer unprecedented opportunities for elucidating the functions of enhancers in their endogenous context. Here, we discuss the application, current limitations and future development of CRISPR–Cas9 systems to identify and characterize enhancer elements in a high-throughput manner.</description><subject>631/1647/2163</subject><subject>631/337/4041/3196</subject><subject>631/337/572/2102</subject><subject>Biochemistry</subject><subject>Cancer Research</subject><subject>Cell Biology</subject><subject>CRISPR</subject><subject>CRISPR-Cas Systems</subject><subject>Developmental Biology</subject><subject>Enhancer Elements, Genetic</subject><subject>Epigenesis, Genetic</subject><subject>Epigenetics</subject><subject>Epigenomics - methods</subject><subject>Gene Editing - methods</subject><subject>Gene expression</subject><subject>Genetic engineering</subject><subject>Genetic research</subject><subject>Genetic transcription</subject><subject>Genomes</subject><subject>Hemoglobin</subject><subject>innovation</subject><subject>Innovations</subject><subject>Life Sciences</subject><subject>Proteins</subject><subject>RNA polymerase</subject><subject>Stem Cells</subject><subject>Transcription factors</subject><issn>1471-0072</issn><issn>1471-0080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqN0kGLEzEUAOAgiruunrzLgBcXbU0ymcnkWMqqhQWl1XNIMy_dLDPJmGTAevI_-A_9JWbsulrZgwTyQvK9By88hJ4SPCe4bF670M8pJvWci3volDBOZhg3-P7tmdMT9CjGa5wR4dVDdEJ52RDKylO0WQxDt7duVyzXq82H9Y9v35cqiiJ538W8F7YFl6zZF8q1hb5SQekEwX6FIgXlog52SNY71RXgrpTTEOJj9MCoLsKTm3iGPr25-Lh8N7t8_3a1XFzOdFWxNGswNlvOaK0IU1Cz0jQVMS0ra8Eb1fIttEAEE7psqcI5KtpoqDTTptZbRsoz9OJQdwj-8wgxyd5GDV2nHPgxStKQimMh8H_RsqakwSLT5__Qaz-G3OAvRQWlFeZ_1E51IK0zPn-HnorKBasJpQzTqdb8DpVXC73V3oGx-f4o4fwoIZsEX9JOjTHK1WZ9bF8erA4-xgBGDsH2KuwlwXKaDJknQ06TIfmkn920NW57aG_t71HI4NUBxPzkdhD-6vuOej8BApC_7g</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Lopes, Rui</creator><creator>Korkmaz, Gozde</creator><creator>Agami, Reuven</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20160901</creationdate><title>Applying CRISPR–Cas9 tools to identify and characterize transcriptional enhancers</title><author>Lopes, Rui ; Korkmaz, Gozde ; Agami, Reuven</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c554t-800fb7426a14ae643f851fd436978ad7bede1949c3d2a049ca28ce5c4cf6cb413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>631/1647/2163</topic><topic>631/337/4041/3196</topic><topic>631/337/572/2102</topic><topic>Biochemistry</topic><topic>Cancer Research</topic><topic>Cell Biology</topic><topic>CRISPR</topic><topic>CRISPR-Cas Systems</topic><topic>Developmental Biology</topic><topic>Enhancer Elements, Genetic</topic><topic>Epigenesis, Genetic</topic><topic>Epigenetics</topic><topic>Epigenomics - methods</topic><topic>Gene Editing - methods</topic><topic>Gene expression</topic><topic>Genetic engineering</topic><topic>Genetic research</topic><topic>Genetic transcription</topic><topic>Genomes</topic><topic>Hemoglobin</topic><topic>innovation</topic><topic>Innovations</topic><topic>Life Sciences</topic><topic>Proteins</topic><topic>RNA polymerase</topic><topic>Stem Cells</topic><topic>Transcription factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lopes, Rui</creatorcontrib><creatorcontrib>Korkmaz, Gozde</creatorcontrib><creatorcontrib>Agami, Reuven</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature reviews. Molecular cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lopes, Rui</au><au>Korkmaz, Gozde</au><au>Agami, Reuven</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Applying CRISPR–Cas9 tools to identify and characterize transcriptional enhancers</atitle><jtitle>Nature reviews. Molecular cell biology</jtitle><stitle>Nat Rev Mol Cell Biol</stitle><addtitle>Nat Rev Mol Cell Biol</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>17</volume><issue>9</issue><spage>597</spage><epage>604</epage><pages>597-604</pages><issn>1471-0072</issn><eissn>1471-0080</eissn><abstract>CRISPR–Cas9-based genome editing tools have been developed recently to study non-coding transcriptional regulatory elements, enabling the characterization of enhancers in their endogenous context. The applications, current limitations and future development of such CRISPR–Cas9 tools are discussed, with emphasis on identifying and characterizing enhancer elements in a high-throughput manner.
The development of the CRISPR–Cas9 system triggered a revolution in the field of genome engineering. Initially, the use of this system was focused on the study of protein-coding genes but, recently, a number of CRISPR–Cas9-based tools have been developed to study non-coding transcriptional regulatory elements. These technological advances offer unprecedented opportunities for elucidating the functions of enhancers in their endogenous context. Here, we discuss the application, current limitations and future development of CRISPR–Cas9 systems to identify and characterize enhancer elements in a high-throughput manner.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27381243</pmid><doi>10.1038/nrm.2016.79</doi><tpages>8</tpages></addata></record> |
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| subjects | 631/1647/2163 631/337/4041/3196 631/337/572/2102 Biochemistry Cancer Research Cell Biology CRISPR CRISPR-Cas Systems Developmental Biology Enhancer Elements, Genetic Epigenesis, Genetic Epigenetics Epigenomics - methods Gene Editing - methods Gene expression Genetic engineering Genetic research Genetic transcription Genomes Hemoglobin innovation Innovations Life Sciences Proteins RNA polymerase Stem Cells Transcription factors |
| title | Applying CRISPR–Cas9 tools to identify and characterize transcriptional enhancers |
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