Characteristics of extracellular polymeric substances from sludge and biofilm in a simultaneous nitrification and denitrification system under high salinity stress
The composition and distribution of extracellular polymeric substance (EPS) both from suspended sludge and attached biofilm were investigated in a simultaneous nitrification and denitrification (SND) system with the increase of the salinity from 1.0 to 3.0 %. Fourier-transform infrared (FTIR) spectr...
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| creator | Zhao, Linting She, Zonglian Jin, Chunji Yang, Shiying Guo, Liang Zhao, Yangguo Gao, Mengchun |
| description | The composition and distribution of extracellular polymeric substance (EPS) both from suspended sludge and attached biofilm were investigated in a simultaneous nitrification and denitrification (SND) system with the increase of the salinity from 1.0 to 3.0 %. Fourier-transform infrared (FTIR) spectroscopy and three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectroscopy were used to examine proteins (PN), polysaccharides (PS) and humic substances (HS) present in EPS. High total nitrogen removal (above 83.9 %) via SND was obtained in the salinity range of 1.0–2.5 %. Total EPS in the sludge increased from 150.2 to 200.6 mg/gVSS with the increase of salinity from 1.0 to 3.0 %, whereas the corresponding values in the biofilm achieved the maximum of 288.6 mg/g VSS at 2.0 % salinity. Dominant composition of EPS was detected as HS in both sludge and biofilm, having the percentages of 50.6–68.6 and 41.1–69.9 % in total EPS, respectively. Both PN and PS contents in soluble EPS (S-EPS), loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) of sludge and biofilm increased with the increased salinity. The FTIR spectrum and 3D-EEM fluorescence spectroscopy of S-EPS, LB-EPS and TB-EPS in the sludge and biofilm showed the changes of functional groups and conformations of the compositions in EPS with the increase of salinity. The results demonstrated that the characteristics of EPS varied from sludge to biofilm. The obtained results could provide a better understanding of the salinity effect on the EPS characteristics in a SND system. |
| doi_str_mv | 10.1007/s00449-016-1613-x |
| format | Article |
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Fourier-transform infrared (FTIR) spectroscopy and three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectroscopy were used to examine proteins (PN), polysaccharides (PS) and humic substances (HS) present in EPS. High total nitrogen removal (above 83.9 %) via SND was obtained in the salinity range of 1.0–2.5 %. Total EPS in the sludge increased from 150.2 to 200.6 mg/gVSS with the increase of salinity from 1.0 to 3.0 %, whereas the corresponding values in the biofilm achieved the maximum of 288.6 mg/g VSS at 2.0 % salinity. Dominant composition of EPS was detected as HS in both sludge and biofilm, having the percentages of 50.6–68.6 and 41.1–69.9 % in total EPS, respectively. Both PN and PS contents in soluble EPS (S-EPS), loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) of sludge and biofilm increased with the increased salinity. The FTIR spectrum and 3D-EEM fluorescence spectroscopy of S-EPS, LB-EPS and TB-EPS in the sludge and biofilm showed the changes of functional groups and conformations of the compositions in EPS with the increase of salinity. The results demonstrated that the characteristics of EPS varied from sludge to biofilm. The obtained results could provide a better understanding of the salinity effect on the EPS characteristics in a SND system.</description><identifier>ISSN: 1615-7591</identifier><identifier>EISSN: 1615-7605</identifier><identifier>DOI: 10.1007/s00449-016-1613-x</identifier><identifier>PMID: 27126502</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Bioengineering ; Biofilms ; Biotechnology ; Chemistry ; Chemistry and Materials Science ; Denitrification ; Environmental Engineering/Biotechnology ; Extracellular matrix ; Fluorescence ; Food Science ; Industrial and Production Engineering ; Industrial Chemistry/Chemical Engineering ; Nitrification ; Nitrogen removal ; Original Paper ; Polymers - chemistry ; Saccharides ; Salinity ; Sewage - chemistry ; Sludge ; Spectroscopy ; Spectroscopy, Fourier Transform Infrared ; Stress, Physiological</subject><ispartof>Bioprocess and biosystems engineering, 2016-09, Vol.39 (9), p.1375-1389</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-b00ddb6451979e858ef1cc88f5b06a679e744c229122eea9cc0e9a47d439778e3</citedby><cites>FETCH-LOGICAL-c508t-b00ddb6451979e858ef1cc88f5b06a679e744c229122eea9cc0e9a47d439778e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00449-016-1613-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00449-016-1613-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27126502$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Linting</creatorcontrib><creatorcontrib>She, Zonglian</creatorcontrib><creatorcontrib>Jin, Chunji</creatorcontrib><creatorcontrib>Yang, Shiying</creatorcontrib><creatorcontrib>Guo, Liang</creatorcontrib><creatorcontrib>Zhao, Yangguo</creatorcontrib><creatorcontrib>Gao, Mengchun</creatorcontrib><title>Characteristics of extracellular polymeric substances from sludge and biofilm in a simultaneous nitrification and denitrification system under high salinity stress</title><title>Bioprocess and biosystems engineering</title><addtitle>Bioprocess Biosyst Eng</addtitle><addtitle>Bioprocess Biosyst Eng</addtitle><description>The composition and distribution of extracellular polymeric substance (EPS) both from suspended sludge and attached biofilm were investigated in a simultaneous nitrification and denitrification (SND) system with the increase of the salinity from 1.0 to 3.0 %. Fourier-transform infrared (FTIR) spectroscopy and three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectroscopy were used to examine proteins (PN), polysaccharides (PS) and humic substances (HS) present in EPS. High total nitrogen removal (above 83.9 %) via SND was obtained in the salinity range of 1.0–2.5 %. Total EPS in the sludge increased from 150.2 to 200.6 mg/gVSS with the increase of salinity from 1.0 to 3.0 %, whereas the corresponding values in the biofilm achieved the maximum of 288.6 mg/g VSS at 2.0 % salinity. Dominant composition of EPS was detected as HS in both sludge and biofilm, having the percentages of 50.6–68.6 and 41.1–69.9 % in total EPS, respectively. Both PN and PS contents in soluble EPS (S-EPS), loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) of sludge and biofilm increased with the increased salinity. The FTIR spectrum and 3D-EEM fluorescence spectroscopy of S-EPS, LB-EPS and TB-EPS in the sludge and biofilm showed the changes of functional groups and conformations of the compositions in EPS with the increase of salinity. The results demonstrated that the characteristics of EPS varied from sludge to biofilm. The obtained results could provide a better understanding of the salinity effect on the EPS characteristics in a SND system.</description><subject>Bioengineering</subject><subject>Biofilms</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Denitrification</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Extracellular matrix</subject><subject>Fluorescence</subject><subject>Food Science</subject><subject>Industrial and Production Engineering</subject><subject>Industrial Chemistry/Chemical Engineering</subject><subject>Nitrification</subject><subject>Nitrogen removal</subject><subject>Original Paper</subject><subject>Polymers - chemistry</subject><subject>Saccharides</subject><subject>Salinity</subject><subject>Sewage - chemistry</subject><subject>Sludge</subject><subject>Spectroscopy</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Stress, Physiological</subject><issn>1615-7591</issn><issn>1615-7605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc9u1DAQxiNERUvhAbggS1y4hI6dOI6PaFX-SJW4wNlynMmuKydZPI60-zx90XqbFgESEiePvvnNNxp_RfGGwwcOoK4IoK51CbwpecOr8vCsuMiFLFUD8vlTLTU_L14S3QJw2Qp4UZwLxUUjQVwUd5udjdYljJ6Sd8TmgeEhZQlDWIKNbD-H45jbjtHSUbKTQ2JDnEdGYem3yOzUs87Pgw8j8xOzjPy4hAzivBCbfIp-8M4mP08PbI9_anSkhCNbph4j2_ntjpENPjNHRiki0avibLCB8PXje1n8-HT9ffOlvPn2-evm403pJLSp7AD6vmtqybXS2MoWB-5c2w6yg8Y2WVN17YTQXAhEq50D1LZWfV1ppVqsLov3q-8-zj8XpGRGT6d_WE8xvOVSga5V-z8oF7rmUGX03V_o7bzEKR_yQIGsKi0zxVfKxZko4mD20Y82Hg0HcwrbrGGbHLY5hW0Oeebto_PSjdj_mnhKNwNiBSi3pi3G31b_0_UejCC5GA</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Zhao, Linting</creator><creator>She, Zonglian</creator><creator>Jin, Chunji</creator><creator>Yang, Shiying</creator><creator>Guo, Liang</creator><creator>Zhao, Yangguo</creator><creator>Gao, Mengchun</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20160901</creationdate><title>Characteristics of extracellular polymeric substances from sludge and biofilm in a simultaneous nitrification and denitrification system under high salinity stress</title><author>Zhao, Linting ; She, Zonglian ; Jin, Chunji ; Yang, Shiying ; Guo, Liang ; Zhao, Yangguo ; Gao, Mengchun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-b00ddb6451979e858ef1cc88f5b06a679e744c229122eea9cc0e9a47d439778e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bioengineering</topic><topic>Biofilms</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Denitrification</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Extracellular matrix</topic><topic>Fluorescence</topic><topic>Food Science</topic><topic>Industrial and Production Engineering</topic><topic>Industrial Chemistry/Chemical Engineering</topic><topic>Nitrification</topic><topic>Nitrogen removal</topic><topic>Original Paper</topic><topic>Polymers - 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Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Bioprocess and biosystems engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Linting</au><au>She, Zonglian</au><au>Jin, Chunji</au><au>Yang, Shiying</au><au>Guo, Liang</au><au>Zhao, Yangguo</au><au>Gao, Mengchun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characteristics of extracellular polymeric substances from sludge and biofilm in a simultaneous nitrification and denitrification system under high salinity stress</atitle><jtitle>Bioprocess and biosystems engineering</jtitle><stitle>Bioprocess Biosyst Eng</stitle><addtitle>Bioprocess Biosyst Eng</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>39</volume><issue>9</issue><spage>1375</spage><epage>1389</epage><pages>1375-1389</pages><issn>1615-7591</issn><eissn>1615-7605</eissn><abstract>The composition and distribution of extracellular polymeric substance (EPS) both from suspended sludge and attached biofilm were investigated in a simultaneous nitrification and denitrification (SND) system with the increase of the salinity from 1.0 to 3.0 %. Fourier-transform infrared (FTIR) spectroscopy and three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectroscopy were used to examine proteins (PN), polysaccharides (PS) and humic substances (HS) present in EPS. High total nitrogen removal (above 83.9 %) via SND was obtained in the salinity range of 1.0–2.5 %. Total EPS in the sludge increased from 150.2 to 200.6 mg/gVSS with the increase of salinity from 1.0 to 3.0 %, whereas the corresponding values in the biofilm achieved the maximum of 288.6 mg/g VSS at 2.0 % salinity. Dominant composition of EPS was detected as HS in both sludge and biofilm, having the percentages of 50.6–68.6 and 41.1–69.9 % in total EPS, respectively. Both PN and PS contents in soluble EPS (S-EPS), loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) of sludge and biofilm increased with the increased salinity. The FTIR spectrum and 3D-EEM fluorescence spectroscopy of S-EPS, LB-EPS and TB-EPS in the sludge and biofilm showed the changes of functional groups and conformations of the compositions in EPS with the increase of salinity. The results demonstrated that the characteristics of EPS varied from sludge to biofilm. The obtained results could provide a better understanding of the salinity effect on the EPS characteristics in a SND system.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27126502</pmid><doi>10.1007/s00449-016-1613-x</doi><tpages>15</tpages></addata></record> |
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| subjects | Bioengineering Biofilms Biotechnology Chemistry Chemistry and Materials Science Denitrification Environmental Engineering/Biotechnology Extracellular matrix Fluorescence Food Science Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Nitrification Nitrogen removal Original Paper Polymers - chemistry Saccharides Salinity Sewage - chemistry Sludge Spectroscopy Spectroscopy, Fourier Transform Infrared Stress, Physiological |
| title | Characteristics of extracellular polymeric substances from sludge and biofilm in a simultaneous nitrification and denitrification system under high salinity stress |
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