Advances in the Development of Gene-Targeting Vectors to Increase the Efficiency of Genetic Modification
Gene targeting via homologous recombination, albeit highly inefficient in human cells, is considered a powerful tool for analyzing gene functions. Despite recent progress in the application of artificial nucleases for genome editing, safety issues remain a concern, particularly when genetic modifica...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2016/01/01, Vol.39(1), pp.25-32 |
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| creator | Saito, Shinta Adachi, Noritaka |
| description | Gene targeting via homologous recombination, albeit highly inefficient in human cells, is considered a powerful tool for analyzing gene functions. Despite recent progress in the application of artificial nucleases for genome editing, safety issues remain a concern, particularly when genetic modification is used for therapeutic purposes. Therefore, the development of gene-targeting vectors is necessary for safe and sophisticated genetic modification. In this paper, we describe the effect of vector structure on random integration, which is a major obstacle in efficient gene targeting. In addition, we focus on the features of exon-trapping-type gene-targeting vectors, and discuss a novel strategy for negative selection to enhance gene targeting in human cells. |
| doi_str_mv | 10.1248/bpb.b15-00701 |
| format | Article |
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| ispartof | Biological and Pharmaceutical Bulletin, 2016/01/01, Vol.39(1), pp.25-32 |
| issn | 0918-6158 1347-5215 |
| language | eng |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | exon-trap Gene Expression Regulation - physiology gene targeting Gene Targeting - methods Genetic Engineering - methods Genetic Vectors Humans random integration targeting vector |
| title | Advances in the Development of Gene-Targeting Vectors to Increase the Efficiency of Genetic Modification |
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