Generation of stomach tissue from mouse embryonic stem cells
Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demon...
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| Veröffentlicht in: | Nature cell biology 2015-08, Vol.17 (8), p.984-993 |
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| creator | Noguchi, Taka-aki K. Ninomiya, Naoto Sekine, Mari Komazaki, Shinji Wang, Pi-Chao Asashima, Makoto Kurisaki, Akira |
| description | Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for stomach differentiation from embryonic stem cells by inducing mesenchymal
Barx1
, an essential gene for
in vivo
stomach specification from gut endoderm.
Barx1
-inducing culture conditions generated stomach primordium-like spheroids, which differentiated into mature stomach tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived stomach tissue (e-ST) shared a similar gene expression profile with adult stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Ménétrier disease
in vitro
. Thus,
in vitro
stomach tissue derived from pluripotent stem cells mimics
in vivo
development and can be used for stomach disease models.
Noguchi and colleagues report the generation of stomach-like tissue from mouse embryonic stem cells. They show that the tissue contains all stomach-specific cell types and secretes acid and digestive enzyme. |
| doi_str_mv | 10.1038/ncb3200 |
| format | Article |
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Barx1
, an essential gene for
in vivo
stomach specification from gut endoderm.
Barx1
-inducing culture conditions generated stomach primordium-like spheroids, which differentiated into mature stomach tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived stomach tissue (e-ST) shared a similar gene expression profile with adult stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Ménétrier disease
in vitro
. Thus,
in vitro
stomach tissue derived from pluripotent stem cells mimics
in vivo
development and can be used for stomach disease models.
Noguchi and colleagues report the generation of stomach-like tissue from mouse embryonic stem cells. They show that the tissue contains all stomach-specific cell types and secretes acid and digestive enzyme.</description><identifier>ISSN: 1465-7392</identifier><identifier>EISSN: 1476-4679</identifier><identifier>DOI: 10.1038/ncb3200</identifier><identifier>PMID: 26192439</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/100 ; 13/106 ; 14/1 ; 14/28 ; 14/63 ; 38/61 ; 631/136/2060 ; 631/136/532/1360 ; 631/136/532/2117 ; 631/136/756/1462 ; 64/60 ; Animals ; Cancer Research ; Cell Biology ; Cell culture ; Cell differentiation ; Cell Differentiation - drug effects ; Cell Lineage ; Cell research ; Developmental Biology ; Embryonic Stem Cells - drug effects ; Embryonic Stem Cells - metabolism ; Embryonic Stem Cells - physiology ; Gastric Acid - metabolism ; Gastric Mucosa - metabolism ; Gastritis, Hypertrophic - genetics ; Gastritis, Hypertrophic - metabolism ; Gastritis, Hypertrophic - pathology ; Gastrointestinal diseases ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Hedgehog Proteins - metabolism ; Histamine - pharmacology ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Innovations ; Life Sciences ; Mice ; Mice, Inbred ICR ; Organogenesis ; Pepsinogen A - metabolism ; Phenotype ; Regenerative Medicine - methods ; Spheroids, Cellular ; Stem Cells ; Stomach - cytology ; Stomach - drug effects ; Stomach - physiology ; Time Factors ; Tissue Engineering - methods ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transfection ; Transforming Growth Factor alpha - genetics ; Transforming Growth Factor alpha - metabolism</subject><ispartof>Nature cell biology, 2015-08, Vol.17 (8), p.984-993</ispartof><rights>Springer Nature Limited 2014</rights><rights>COPYRIGHT 2015 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Aug 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c542t-8e47ac8fc0111ad38719c57ddf0dc784ff7d99307076e985a048b6c85b029d323</citedby><cites>FETCH-LOGICAL-c542t-8e47ac8fc0111ad38719c57ddf0dc784ff7d99307076e985a048b6c85b029d323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26192439$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Noguchi, Taka-aki K.</creatorcontrib><creatorcontrib>Ninomiya, Naoto</creatorcontrib><creatorcontrib>Sekine, Mari</creatorcontrib><creatorcontrib>Komazaki, Shinji</creatorcontrib><creatorcontrib>Wang, Pi-Chao</creatorcontrib><creatorcontrib>Asashima, Makoto</creatorcontrib><creatorcontrib>Kurisaki, Akira</creatorcontrib><title>Generation of stomach tissue from mouse embryonic stem cells</title><title>Nature cell biology</title><addtitle>Nat Cell Biol</addtitle><addtitle>Nat Cell Biol</addtitle><description>Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for stomach differentiation from embryonic stem cells by inducing mesenchymal
Barx1
, an essential gene for
in vivo
stomach specification from gut endoderm.
Barx1
-inducing culture conditions generated stomach primordium-like spheroids, which differentiated into mature stomach tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived stomach tissue (e-ST) shared a similar gene expression profile with adult stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Ménétrier disease
in vitro
. Thus,
in vitro
stomach tissue derived from pluripotent stem cells mimics
in vivo
development and can be used for stomach disease models.
Noguchi and colleagues report the generation of stomach-like tissue from mouse embryonic stem cells. They show that the tissue contains all stomach-specific cell types and secretes acid and digestive enzyme.</description><subject>13/100</subject><subject>13/106</subject><subject>14/1</subject><subject>14/28</subject><subject>14/63</subject><subject>38/61</subject><subject>631/136/2060</subject><subject>631/136/532/1360</subject><subject>631/136/532/2117</subject><subject>631/136/756/1462</subject><subject>64/60</subject><subject>Animals</subject><subject>Cancer Research</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Lineage</subject><subject>Cell research</subject><subject>Developmental Biology</subject><subject>Embryonic Stem Cells - drug effects</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Embryonic Stem Cells - physiology</subject><subject>Gastric Acid - metabolism</subject><subject>Gastric Mucosa - metabolism</subject><subject>Gastritis, Hypertrophic - genetics</subject><subject>Gastritis, Hypertrophic - metabolism</subject><subject>Gastritis, Hypertrophic - pathology</subject><subject>Gastrointestinal diseases</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Hedgehog Proteins - metabolism</subject><subject>Histamine - pharmacology</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Innovations</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Organogenesis</subject><subject>Pepsinogen A - metabolism</subject><subject>Phenotype</subject><subject>Regenerative Medicine - methods</subject><subject>Spheroids, Cellular</subject><subject>Stem Cells</subject><subject>Stomach - cytology</subject><subject>Stomach - drug effects</subject><subject>Stomach - physiology</subject><subject>Time Factors</subject><subject>Tissue Engineering - methods</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transfection</subject><subject>Transforming Growth Factor alpha - genetics</subject><subject>Transforming Growth Factor alpha - metabolism</subject><issn>1465-7392</issn><issn>1476-4679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkVtrFTEUhYMovVn8BzLgg-3D1NwmF_CllLYWCoLV55DJ7BynnCQ1mQH7783Q09ZTBMlDQvKtxV5ZCL0j-IRgpj5F1zOK8Su0R7gULRdSv17Oomsl03QX7ZdyizHhHMsdtEsF0ZQzvYc-X0KEbKcxxSb5pkwpWPezmcZSZmh8TqEJaS7QQOjzfYqjqwyExsF6Xd6iN96uCxxu9gP04-L8-9mX9vrr5dXZ6XXrOk6nVgGX1invMCHEDkxJol0nh8HjwUnFvZeD1gxLLAVo1VnMVS-c6npM9cAoO0BHD753Of2aoUwmjGWZwEaowxmiSFfFSqr_oxITpbGWi-uHF-htmnOsQSolBGFCaflMrewazBh9mrJ1i6k55bQTssNUVOrkH1RdA4TRpQh-rPdbguMtQWUm-D2t7FyKubr5ts1-fGBdTqVk8OYuj8Hme0OwWeo3m_or-X4Tae4DDE_cY9_P31PqU1xB_ivzC68_gnSy1w</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Noguchi, Taka-aki K.</creator><creator>Ninomiya, Naoto</creator><creator>Sekine, Mari</creator><creator>Komazaki, Shinji</creator><creator>Wang, Pi-Chao</creator><creator>Asashima, Makoto</creator><creator>Kurisaki, Akira</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20150801</creationdate><title>Generation of stomach tissue from mouse embryonic stem cells</title><author>Noguchi, Taka-aki K. ; Ninomiya, Naoto ; Sekine, Mari ; Komazaki, Shinji ; Wang, Pi-Chao ; Asashima, Makoto ; Kurisaki, Akira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c542t-8e47ac8fc0111ad38719c57ddf0dc784ff7d99307076e985a048b6c85b029d323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>13/100</topic><topic>13/106</topic><topic>14/1</topic><topic>14/28</topic><topic>14/63</topic><topic>38/61</topic><topic>631/136/2060</topic><topic>631/136/532/1360</topic><topic>631/136/532/2117</topic><topic>631/136/756/1462</topic><topic>64/60</topic><topic>Animals</topic><topic>Cancer Research</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - 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methods</topic><topic>Spheroids, Cellular</topic><topic>Stem Cells</topic><topic>Stomach - cytology</topic><topic>Stomach - drug effects</topic><topic>Stomach - physiology</topic><topic>Time Factors</topic><topic>Tissue Engineering - methods</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transfection</topic><topic>Transforming Growth Factor alpha - genetics</topic><topic>Transforming Growth Factor alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Noguchi, Taka-aki K.</creatorcontrib><creatorcontrib>Ninomiya, Naoto</creatorcontrib><creatorcontrib>Sekine, Mari</creatorcontrib><creatorcontrib>Komazaki, Shinji</creatorcontrib><creatorcontrib>Wang, Pi-Chao</creatorcontrib><creatorcontrib>Asashima, Makoto</creatorcontrib><creatorcontrib>Kurisaki, Akira</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Noguchi, Taka-aki K.</au><au>Ninomiya, Naoto</au><au>Sekine, Mari</au><au>Komazaki, Shinji</au><au>Wang, Pi-Chao</au><au>Asashima, Makoto</au><au>Kurisaki, Akira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of stomach tissue from mouse embryonic stem cells</atitle><jtitle>Nature cell biology</jtitle><stitle>Nat Cell Biol</stitle><addtitle>Nat Cell Biol</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>17</volume><issue>8</issue><spage>984</spage><epage>993</epage><pages>984-993</pages><issn>1465-7392</issn><eissn>1476-4679</eissn><abstract>Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for stomach differentiation from embryonic stem cells by inducing mesenchymal
Barx1
, an essential gene for
in vivo
stomach specification from gut endoderm.
Barx1
-inducing culture conditions generated stomach primordium-like spheroids, which differentiated into mature stomach tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived stomach tissue (e-ST) shared a similar gene expression profile with adult stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Ménétrier disease
in vitro
. Thus,
in vitro
stomach tissue derived from pluripotent stem cells mimics
in vivo
development and can be used for stomach disease models.
Noguchi and colleagues report the generation of stomach-like tissue from mouse embryonic stem cells. They show that the tissue contains all stomach-specific cell types and secretes acid and digestive enzyme.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26192439</pmid><doi>10.1038/ncb3200</doi><tpages>10</tpages></addata></record> |
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| ispartof | Nature cell biology, 2015-08, Vol.17 (8), p.984-993 |
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| subjects | 13/100 13/106 14/1 14/28 14/63 38/61 631/136/2060 631/136/532/1360 631/136/532/2117 631/136/756/1462 64/60 Animals Cancer Research Cell Biology Cell culture Cell differentiation Cell Differentiation - drug effects Cell Lineage Cell research Developmental Biology Embryonic Stem Cells - drug effects Embryonic Stem Cells - metabolism Embryonic Stem Cells - physiology Gastric Acid - metabolism Gastric Mucosa - metabolism Gastritis, Hypertrophic - genetics Gastritis, Hypertrophic - metabolism Gastritis, Hypertrophic - pathology Gastrointestinal diseases Gene Expression Profiling Gene Expression Regulation, Developmental Hedgehog Proteins - metabolism Histamine - pharmacology Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Innovations Life Sciences Mice Mice, Inbred ICR Organogenesis Pepsinogen A - metabolism Phenotype Regenerative Medicine - methods Spheroids, Cellular Stem Cells Stomach - cytology Stomach - drug effects Stomach - physiology Time Factors Tissue Engineering - methods Transcription Factors - genetics Transcription Factors - metabolism Transfection Transforming Growth Factor alpha - genetics Transforming Growth Factor alpha - metabolism |
| title | Generation of stomach tissue from mouse embryonic stem cells |
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