Generation of Rat Induced Pluripotent Stem Cells Using a Plasmid Vector and Possible Application of a Keratan Sulfate Glycan Recognizing Antibody in Discriminating Teratoma Formation Phenotypes
Induced pluripotent stem cells (iPSCs) offer an invaluable tool for biological research and regenerative medicine. We report establishment of rat iPSCs (riPSCs) using a plasmid vector encoding four transcription factors, Oct3/4, Sox2, c-Myc and Klf4. Although all riPSC clones were generated and cult...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2015/01/01, Vol.38(1), pp.127-133 |
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| creator | Makanga, Juliet O. Kobayashi, Misa Ikeda, Hiroki Christianto, Antonius Toyoda, Hidenao Yamada, Mitsunori Kawasaki, Toshisuke Inazu, Tetsuya |
| description | Induced pluripotent stem cells (iPSCs) offer an invaluable tool for biological research and regenerative medicine. We report establishment of rat iPSCs (riPSCs) using a plasmid vector encoding four transcription factors, Oct3/4, Sox2, c-Myc and Klf4. Although all riPSC clones were generated and cultured under the same conditions, expressed hallmark pluripotency markers and differentiated successfully in vitro, the expression of a keratan sulfate glycan epitope with unique properties defined by R-10G antibody varied in the riPSC clones. In contrast, tumor rejection antigen (TRA)-1-81 epitope expression was comparable. A clone highly reactive to R-10G antibody formed teratomas in vivo consisting of cells from all three germ layers. However, clones expressing a lower level of the epitope defined by R-10G resulted in tumors with rapid growth consisting of undifferentiated cells. Additionally, riPSCs could be successfully differentiated into a neuronal lineage including glutamate neurons that responded to agonist stimulation. These observations demonstrate a glycophenotypic difference that may potentially serve as a useful probe for riPSC evaluation and to study the role of glycans in pluripotency and carcinogenesis in these cells. |
| doi_str_mv | 10.1248/bpb.b14-00697 |
| format | Article |
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We report establishment of rat iPSCs (riPSCs) using a plasmid vector encoding four transcription factors, Oct3/4, Sox2, c-Myc and Klf4. Although all riPSC clones were generated and cultured under the same conditions, expressed hallmark pluripotency markers and differentiated successfully in vitro, the expression of a keratan sulfate glycan epitope with unique properties defined by R-10G antibody varied in the riPSC clones. In contrast, tumor rejection antigen (TRA)-1-81 epitope expression was comparable. A clone highly reactive to R-10G antibody formed teratomas in vivo consisting of cells from all three germ layers. However, clones expressing a lower level of the epitope defined by R-10G resulted in tumors with rapid growth consisting of undifferentiated cells. Additionally, riPSCs could be successfully differentiated into a neuronal lineage including glutamate neurons that responded to agonist stimulation. These observations demonstrate a glycophenotypic difference that may potentially serve as a useful probe for riPSC evaluation and to study the role of glycans in pluripotency and carcinogenesis in these cells.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.b14-00697</identifier><identifier>PMID: 25744468</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Animals ; Antibodies - immunology ; Antigens, Surface - immunology ; Cell Differentiation ; glycobiology ; induced pluripotent stem cell ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - metabolism ; Keratan Sulfate - immunology ; Kruppel-Like Transcription Factors - metabolism ; Male ; Mice, Inbred BALB C ; Octamer Transcription Factor-3 - metabolism ; Phenotype ; Plasmids ; Proto-Oncogene Proteins c-myc - metabolism ; R-10G antibody ; Rats, Wistar ; SOXB1 Transcription Factors - metabolism ; Teratoma</subject><ispartof>Biological and Pharmaceutical Bulletin, 2015/01/01, Vol.38(1), pp.127-133</ispartof><rights>2015 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c642t-ae643d9fed44d03232808b02df7eaa1f79a8d8d5cb91e6a39807ee93ea7b4cbb3</citedby><cites>FETCH-LOGICAL-c642t-ae643d9fed44d03232808b02df7eaa1f79a8d8d5cb91e6a39807ee93ea7b4cbb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25744468$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Makanga, Juliet O.</creatorcontrib><creatorcontrib>Kobayashi, Misa</creatorcontrib><creatorcontrib>Ikeda, Hiroki</creatorcontrib><creatorcontrib>Christianto, Antonius</creatorcontrib><creatorcontrib>Toyoda, Hidenao</creatorcontrib><creatorcontrib>Yamada, Mitsunori</creatorcontrib><creatorcontrib>Kawasaki, Toshisuke</creatorcontrib><creatorcontrib>Inazu, Tetsuya</creatorcontrib><creatorcontrib>aLaboratory of Functional Genomics</creatorcontrib><creatorcontrib>bLaboratory of Bio-analytical Chemistry</creatorcontrib><creatorcontrib>College of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>dResearch Center for Glycobiotechnology</creatorcontrib><creatorcontrib>Ritsumeikan University</creatorcontrib><creatorcontrib>NHO</creatorcontrib><creatorcontrib>Saigata Medical Center</creatorcontrib><creatorcontrib>Department of Clinical Research</creatorcontrib><creatorcontrib>cLaboratory of Neuropathology</creatorcontrib><title>Generation of Rat Induced Pluripotent Stem Cells Using a Plasmid Vector and Possible Application of a Keratan Sulfate Glycan Recognizing Antibody in Discriminating Teratoma Formation Phenotypes</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Induced pluripotent stem cells (iPSCs) offer an invaluable tool for biological research and regenerative medicine. We report establishment of rat iPSCs (riPSCs) using a plasmid vector encoding four transcription factors, Oct3/4, Sox2, c-Myc and Klf4. Although all riPSC clones were generated and cultured under the same conditions, expressed hallmark pluripotency markers and differentiated successfully in vitro, the expression of a keratan sulfate glycan epitope with unique properties defined by R-10G antibody varied in the riPSC clones. In contrast, tumor rejection antigen (TRA)-1-81 epitope expression was comparable. A clone highly reactive to R-10G antibody formed teratomas in vivo consisting of cells from all three germ layers. However, clones expressing a lower level of the epitope defined by R-10G resulted in tumors with rapid growth consisting of undifferentiated cells. Additionally, riPSCs could be successfully differentiated into a neuronal lineage including glutamate neurons that responded to agonist stimulation. These observations demonstrate a glycophenotypic difference that may potentially serve as a useful probe for riPSC evaluation and to study the role of glycans in pluripotency and carcinogenesis in these cells.</description><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Antigens, Surface - immunology</subject><subject>Cell Differentiation</subject><subject>glycobiology</subject><subject>induced pluripotent stem cell</subject><subject>Induced Pluripotent Stem Cells - cytology</subject><subject>Induced Pluripotent Stem Cells - metabolism</subject><subject>Keratan Sulfate - immunology</subject><subject>Kruppel-Like Transcription Factors - metabolism</subject><subject>Male</subject><subject>Mice, Inbred BALB C</subject><subject>Octamer Transcription Factor-3 - metabolism</subject><subject>Phenotype</subject><subject>Plasmids</subject><subject>Proto-Oncogene Proteins c-myc - metabolism</subject><subject>R-10G antibody</subject><subject>Rats, Wistar</subject><subject>SOXB1 Transcription Factors - metabolism</subject><subject>Teratoma</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhiMEoqVw5IosceGSYsdx4hxXC10qKlH1g6tlO5OtV44dbOew_Dv-GU63LBIXDh7LmtfPjF9PUbwl-JxUNf-oJnWuSF1i3HTts-KU0LotWUXY8-IUd4SXDWH8pHgV4w5j3OKKvixOKtbWdd3w0-LXBhwEmYx3yA_oRiZ06fpZQ4-u7RzM5BO4hG4TjGgN1kZ0H43bIpnTMo6mR99BJx-QdPmGj9EoC2g1TdboI1Wir0sN6dDtbAeZAG3sXufjDWi_debnQly5ZJTv98g49MlEHcxoXEbk1N1y248SXfgwHqjXD-B82k8QXxcvBmkjvHnaz4r7i8936y_l1bfN5Xp1VeqmrlIpoalp3w3Q13WPaUUrjrnCVT-0ICUZ2k7ynvdMq45AI2nHcQvQUZCtqrVS9Kz4cOBOwf-YISYx5i6zJdKBn6MgnLCmw9n__0ubhlBKCa-y9P0_0p2fg8sPEaRllLGOkgVYHlQ6ZIsDDGLK9siwFwSLZQxEHgORx0A8jkHWv3uizmqE_qj-8-9ZsDkIcjZ_lPXOGgd_a-vYKuOtFxUmLEMpx0Rg0uVVtTnQbCDnDLNMWh9Iu5jkFo6lZEhGW3hsjHJBlnBs8JjVDzIIcPQ3Xo7fsA</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Makanga, Juliet O.</creator><creator>Kobayashi, Misa</creator><creator>Ikeda, Hiroki</creator><creator>Christianto, Antonius</creator><creator>Toyoda, Hidenao</creator><creator>Yamada, Mitsunori</creator><creator>Kawasaki, Toshisuke</creator><creator>Inazu, Tetsuya</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><scope>RC3</scope></search><sort><creationdate>20150101</creationdate><title>Generation of Rat Induced Pluripotent Stem Cells Using a Plasmid Vector and Possible Application of a Keratan Sulfate Glycan Recognizing Antibody in Discriminating Teratoma Formation Phenotypes</title><author>Makanga, Juliet O. ; 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We report establishment of rat iPSCs (riPSCs) using a plasmid vector encoding four transcription factors, Oct3/4, Sox2, c-Myc and Klf4. Although all riPSC clones were generated and cultured under the same conditions, expressed hallmark pluripotency markers and differentiated successfully in vitro, the expression of a keratan sulfate glycan epitope with unique properties defined by R-10G antibody varied in the riPSC clones. In contrast, tumor rejection antigen (TRA)-1-81 epitope expression was comparable. A clone highly reactive to R-10G antibody formed teratomas in vivo consisting of cells from all three germ layers. However, clones expressing a lower level of the epitope defined by R-10G resulted in tumors with rapid growth consisting of undifferentiated cells. Additionally, riPSCs could be successfully differentiated into a neuronal lineage including glutamate neurons that responded to agonist stimulation. These observations demonstrate a glycophenotypic difference that may potentially serve as a useful probe for riPSC evaluation and to study the role of glycans in pluripotency and carcinogenesis in these cells.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>25744468</pmid><doi>10.1248/bpb.b14-00697</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies - immunology Antigens, Surface - immunology Cell Differentiation glycobiology induced pluripotent stem cell Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Keratan Sulfate - immunology Kruppel-Like Transcription Factors - metabolism Male Mice, Inbred BALB C Octamer Transcription Factor-3 - metabolism Phenotype Plasmids Proto-Oncogene Proteins c-myc - metabolism R-10G antibody Rats, Wistar SOXB1 Transcription Factors - metabolism Teratoma |
| title | Generation of Rat Induced Pluripotent Stem Cells Using a Plasmid Vector and Possible Application of a Keratan Sulfate Glycan Recognizing Antibody in Discriminating Teratoma Formation Phenotypes |
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