Thermoresponsive mesoporous silica nanoparticles as a carrier for skin delivery of quercetin
[Display omitted] Copolymer-grafted MSNs to control the release of quercetin on the skin.Matrix biocompatibility in vitro assessed on human keratinocyte cell line.Q complex antioxidant efficacy confirmed by DPPH and Fe2+-Ferrozine assays.Porcine skin accumulation of Q evaluated by Franz cells. R...
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Veröffentlicht in: | International journal of pharmaceutics 2016-09, Vol.511 (1), p.446-454 |
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creator | Ugazio, Elena Gastaldi, Lucia Brunella, Valentina Scalarone, Dominique Jadhav, Sushilkumar A. Oliaro-Bosso, Simonetta Zonari, Daniele Berlier, Gloria Miletto, Ivana Sapino, Simona |
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Copolymer-grafted MSNs to control the release of quercetin on the skin.Matrix biocompatibility in vitro assessed on human keratinocyte cell line.Q complex antioxidant efficacy confirmed by DPPH and Fe2+-Ferrozine assays.Porcine skin accumulation of Q evaluated by Franz cells.
Recently, mesoporous silica nanoparticles (MSNs) have emerged as promising drug delivery systems able to preserve the integrity of the carried substance and/or to selectively reach a target site; however, they have rarely been explored for skin application. In this study, thermoresponsive MSNs, designed to work at physiologic cutaneous temperature, are proposed as innovative topical carriers for quercetin (Q), a well-known antioxidant. The thermosensitive nanoparticles were prepared by functionalizing two different types of matrices, with pore size of 3.5nm (MSNsmall) and 5.0nm (MSNbig), carrying out a free radical copolymerization of N-isopropylacrylamide (NIPAM) and 3-(methacryloxypropyl)trimethoxysilane (MPS) inside the mesopores. The obtained copolymer-grafted MSNs (copoly-MSNs) were physico-chemically characterized and their biocompatibility was attested on a human keratinocyte cell line (HaCaT). The release profiles were assessed and the functional activity of Q, free or loaded, was evaluated in terms of antiradical and metal chelating activities. Ex vivo accumulation and permeation through porcine skin were also investigated. The characterization confirmed the copolymer functionalization of the MSNs. In addition, both the bare and functionalized silica matrices were found to be biocompatible. Among the copolymer-grafted complexes, Q/copoly-MSNbig exhibited more evident thermoresponsive behavior proving the potential of these thermosensitive systems for advanced dermal delivery. |
doi_str_mv | 10.1016/j.ijpharm.2016.07.024 |
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Copolymer-grafted MSNs to control the release of quercetin on the skin.Matrix biocompatibility in vitro assessed on human keratinocyte cell line.Q complex antioxidant efficacy confirmed by DPPH and Fe2+-Ferrozine assays.Porcine skin accumulation of Q evaluated by Franz cells.
Recently, mesoporous silica nanoparticles (MSNs) have emerged as promising drug delivery systems able to preserve the integrity of the carried substance and/or to selectively reach a target site; however, they have rarely been explored for skin application. In this study, thermoresponsive MSNs, designed to work at physiologic cutaneous temperature, are proposed as innovative topical carriers for quercetin (Q), a well-known antioxidant. The thermosensitive nanoparticles were prepared by functionalizing two different types of matrices, with pore size of 3.5nm (MSNsmall) and 5.0nm (MSNbig), carrying out a free radical copolymerization of N-isopropylacrylamide (NIPAM) and 3-(methacryloxypropyl)trimethoxysilane (MPS) inside the mesopores. The obtained copolymer-grafted MSNs (copoly-MSNs) were physico-chemically characterized and their biocompatibility was attested on a human keratinocyte cell line (HaCaT). The release profiles were assessed and the functional activity of Q, free or loaded, was evaluated in terms of antiradical and metal chelating activities. Ex vivo accumulation and permeation through porcine skin were also investigated. The characterization confirmed the copolymer functionalization of the MSNs. In addition, both the bare and functionalized silica matrices were found to be biocompatible. Among the copolymer-grafted complexes, Q/copoly-MSNbig exhibited more evident thermoresponsive behavior proving the potential of these thermosensitive systems for advanced dermal delivery.</description><identifier>ISSN: 0378-5173</identifier><identifier>EISSN: 1873-3476</identifier><identifier>DOI: 10.1016/j.ijpharm.2016.07.024</identifier><identifier>PMID: 27421910</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Administration, Cutaneous ; Animals ; Antioxidant ; Antioxidants - administration & dosage ; Antioxidants - metabolism ; Biocompatibility ; Cell Line, Transformed ; Controlled release ; Copolymer ; Cutaneous application ; Drug Carriers - administration & dosage ; Drug Carriers - metabolism ; Drug Delivery Systems - methods ; Humans ; MCM-41 ; Nanoparticles - administration & dosage ; Nanoparticles - metabolism ; NIPAM ; Organ Culture Techniques ; Porosity ; Quercetin - administration & dosage ; Quercetin - metabolism ; Silicon Dioxide - administration & dosage ; Skin - drug effects ; Skin - metabolism ; Swine</subject><ispartof>International journal of pharmaceutics, 2016-09, Vol.511 (1), p.446-454</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-13917b3d7e90a309d82bc4af80baf451524bffded9fae8a7bf21190d119de1ef3</citedby><cites>FETCH-LOGICAL-c365t-13917b3d7e90a309d82bc4af80baf451524bffded9fae8a7bf21190d119de1ef3</cites><orcidid>0000-0001-5335-8442</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijpharm.2016.07.024$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27421910$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ugazio, Elena</creatorcontrib><creatorcontrib>Gastaldi, Lucia</creatorcontrib><creatorcontrib>Brunella, Valentina</creatorcontrib><creatorcontrib>Scalarone, Dominique</creatorcontrib><creatorcontrib>Jadhav, Sushilkumar A.</creatorcontrib><creatorcontrib>Oliaro-Bosso, Simonetta</creatorcontrib><creatorcontrib>Zonari, Daniele</creatorcontrib><creatorcontrib>Berlier, Gloria</creatorcontrib><creatorcontrib>Miletto, Ivana</creatorcontrib><creatorcontrib>Sapino, Simona</creatorcontrib><title>Thermoresponsive mesoporous silica nanoparticles as a carrier for skin delivery of quercetin</title><title>International journal of pharmaceutics</title><addtitle>Int J Pharm</addtitle><description>[Display omitted]
Copolymer-grafted MSNs to control the release of quercetin on the skin.Matrix biocompatibility in vitro assessed on human keratinocyte cell line.Q complex antioxidant efficacy confirmed by DPPH and Fe2+-Ferrozine assays.Porcine skin accumulation of Q evaluated by Franz cells.
Recently, mesoporous silica nanoparticles (MSNs) have emerged as promising drug delivery systems able to preserve the integrity of the carried substance and/or to selectively reach a target site; however, they have rarely been explored for skin application. In this study, thermoresponsive MSNs, designed to work at physiologic cutaneous temperature, are proposed as innovative topical carriers for quercetin (Q), a well-known antioxidant. The thermosensitive nanoparticles were prepared by functionalizing two different types of matrices, with pore size of 3.5nm (MSNsmall) and 5.0nm (MSNbig), carrying out a free radical copolymerization of N-isopropylacrylamide (NIPAM) and 3-(methacryloxypropyl)trimethoxysilane (MPS) inside the mesopores. The obtained copolymer-grafted MSNs (copoly-MSNs) were physico-chemically characterized and their biocompatibility was attested on a human keratinocyte cell line (HaCaT). The release profiles were assessed and the functional activity of Q, free or loaded, was evaluated in terms of antiradical and metal chelating activities. Ex vivo accumulation and permeation through porcine skin were also investigated. The characterization confirmed the copolymer functionalization of the MSNs. In addition, both the bare and functionalized silica matrices were found to be biocompatible. Among the copolymer-grafted complexes, Q/copoly-MSNbig exhibited more evident thermoresponsive behavior proving the potential of these thermosensitive systems for advanced dermal delivery.</description><subject>Administration, Cutaneous</subject><subject>Animals</subject><subject>Antioxidant</subject><subject>Antioxidants - administration & dosage</subject><subject>Antioxidants - metabolism</subject><subject>Biocompatibility</subject><subject>Cell Line, Transformed</subject><subject>Controlled release</subject><subject>Copolymer</subject><subject>Cutaneous application</subject><subject>Drug Carriers - administration & dosage</subject><subject>Drug Carriers - metabolism</subject><subject>Drug Delivery Systems - methods</subject><subject>Humans</subject><subject>MCM-41</subject><subject>Nanoparticles - administration & dosage</subject><subject>Nanoparticles - metabolism</subject><subject>NIPAM</subject><subject>Organ Culture Techniques</subject><subject>Porosity</subject><subject>Quercetin - administration & dosage</subject><subject>Quercetin - metabolism</subject><subject>Silicon Dioxide - administration & dosage</subject><subject>Skin - drug effects</subject><subject>Skin - metabolism</subject><subject>Swine</subject><issn>0378-5173</issn><issn>1873-3476</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkFFL5TAQhYOs6NXdn7CSx31pzTRt0z7JIu4qCL7o20JIkwnmbtvUSa_gv9_Ivfq6MMwwcM4c5mPsO4gSBLSX2zJsl2dDU1nltRSqFFV9xDbQKVnIWrVf2EZI1RUNKHnKzlLaCiHaCuQJO61UXUEPYsP-PD4jTZEwLXFO4RX5hCkukeIu8RTGYA2fzRwXQ2uwIyZucnFriAIS95F4-htm7nDMZnrj0fOXHZLFNcxf2bE3Y8Jvh3nOnn7dPF7fFvcPv--uf94XVrbNWoDsQQ3SKeyFkaJ3XTXY2vhODMbXDTRVPXjv0PXeYGfU4CuAXrjcHAJ6ec5-7O8uFHN4WvUUksVxNDPmPzR00Mi2bxqRpc1eaimmROj1QmEy9KZB6HeweqsPYPU7WC2UzmCz7-IQsRsmdJ-uD5JZcLUXYH70NbPRyQacLbpAaFftYvhPxD8E8Y70</recordid><startdate>20160910</startdate><enddate>20160910</enddate><creator>Ugazio, Elena</creator><creator>Gastaldi, Lucia</creator><creator>Brunella, Valentina</creator><creator>Scalarone, Dominique</creator><creator>Jadhav, Sushilkumar A.</creator><creator>Oliaro-Bosso, Simonetta</creator><creator>Zonari, Daniele</creator><creator>Berlier, Gloria</creator><creator>Miletto, Ivana</creator><creator>Sapino, Simona</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5335-8442</orcidid></search><sort><creationdate>20160910</creationdate><title>Thermoresponsive mesoporous silica nanoparticles as a carrier for skin delivery of quercetin</title><author>Ugazio, Elena ; Gastaldi, Lucia ; Brunella, Valentina ; Scalarone, Dominique ; Jadhav, Sushilkumar A. ; Oliaro-Bosso, Simonetta ; Zonari, Daniele ; Berlier, Gloria ; Miletto, Ivana ; Sapino, Simona</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-13917b3d7e90a309d82bc4af80baf451524bffded9fae8a7bf21190d119de1ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Administration, Cutaneous</topic><topic>Animals</topic><topic>Antioxidant</topic><topic>Antioxidants - administration & dosage</topic><topic>Antioxidants - metabolism</topic><topic>Biocompatibility</topic><topic>Cell Line, Transformed</topic><topic>Controlled release</topic><topic>Copolymer</topic><topic>Cutaneous application</topic><topic>Drug Carriers - administration & dosage</topic><topic>Drug Carriers - metabolism</topic><topic>Drug Delivery Systems - methods</topic><topic>Humans</topic><topic>MCM-41</topic><topic>Nanoparticles - administration & dosage</topic><topic>Nanoparticles - metabolism</topic><topic>NIPAM</topic><topic>Organ Culture Techniques</topic><topic>Porosity</topic><topic>Quercetin - administration & dosage</topic><topic>Quercetin - metabolism</topic><topic>Silicon Dioxide - administration & dosage</topic><topic>Skin - drug effects</topic><topic>Skin - metabolism</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ugazio, Elena</creatorcontrib><creatorcontrib>Gastaldi, Lucia</creatorcontrib><creatorcontrib>Brunella, Valentina</creatorcontrib><creatorcontrib>Scalarone, Dominique</creatorcontrib><creatorcontrib>Jadhav, Sushilkumar A.</creatorcontrib><creatorcontrib>Oliaro-Bosso, Simonetta</creatorcontrib><creatorcontrib>Zonari, Daniele</creatorcontrib><creatorcontrib>Berlier, Gloria</creatorcontrib><creatorcontrib>Miletto, Ivana</creatorcontrib><creatorcontrib>Sapino, Simona</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of pharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ugazio, Elena</au><au>Gastaldi, Lucia</au><au>Brunella, Valentina</au><au>Scalarone, Dominique</au><au>Jadhav, Sushilkumar A.</au><au>Oliaro-Bosso, Simonetta</au><au>Zonari, Daniele</au><au>Berlier, Gloria</au><au>Miletto, Ivana</au><au>Sapino, Simona</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thermoresponsive mesoporous silica nanoparticles as a carrier for skin delivery of quercetin</atitle><jtitle>International journal of pharmaceutics</jtitle><addtitle>Int J Pharm</addtitle><date>2016-09-10</date><risdate>2016</risdate><volume>511</volume><issue>1</issue><spage>446</spage><epage>454</epage><pages>446-454</pages><issn>0378-5173</issn><eissn>1873-3476</eissn><abstract>[Display omitted]
Copolymer-grafted MSNs to control the release of quercetin on the skin.Matrix biocompatibility in vitro assessed on human keratinocyte cell line.Q complex antioxidant efficacy confirmed by DPPH and Fe2+-Ferrozine assays.Porcine skin accumulation of Q evaluated by Franz cells.
Recently, mesoporous silica nanoparticles (MSNs) have emerged as promising drug delivery systems able to preserve the integrity of the carried substance and/or to selectively reach a target site; however, they have rarely been explored for skin application. In this study, thermoresponsive MSNs, designed to work at physiologic cutaneous temperature, are proposed as innovative topical carriers for quercetin (Q), a well-known antioxidant. The thermosensitive nanoparticles were prepared by functionalizing two different types of matrices, with pore size of 3.5nm (MSNsmall) and 5.0nm (MSNbig), carrying out a free radical copolymerization of N-isopropylacrylamide (NIPAM) and 3-(methacryloxypropyl)trimethoxysilane (MPS) inside the mesopores. The obtained copolymer-grafted MSNs (copoly-MSNs) were physico-chemically characterized and their biocompatibility was attested on a human keratinocyte cell line (HaCaT). The release profiles were assessed and the functional activity of Q, free or loaded, was evaluated in terms of antiradical and metal chelating activities. Ex vivo accumulation and permeation through porcine skin were also investigated. The characterization confirmed the copolymer functionalization of the MSNs. In addition, both the bare and functionalized silica matrices were found to be biocompatible. Among the copolymer-grafted complexes, Q/copoly-MSNbig exhibited more evident thermoresponsive behavior proving the potential of these thermosensitive systems for advanced dermal delivery.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27421910</pmid><doi>10.1016/j.ijpharm.2016.07.024</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-5335-8442</orcidid></addata></record> |
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subjects | Administration, Cutaneous Animals Antioxidant Antioxidants - administration & dosage Antioxidants - metabolism Biocompatibility Cell Line, Transformed Controlled release Copolymer Cutaneous application Drug Carriers - administration & dosage Drug Carriers - metabolism Drug Delivery Systems - methods Humans MCM-41 Nanoparticles - administration & dosage Nanoparticles - metabolism NIPAM Organ Culture Techniques Porosity Quercetin - administration & dosage Quercetin - metabolism Silicon Dioxide - administration & dosage Skin - drug effects Skin - metabolism Swine |
title | Thermoresponsive mesoporous silica nanoparticles as a carrier for skin delivery of quercetin |
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