Fascin 1 promoted the growth and migration of non-small cell lung cancer cells by activating YAP/TEAD signaling
Fascin 1 (Fascin actin-bundling protein 1) is an actin-binding protein. Although several studies have reported the dysregulation of Fascin 1 in non-small cell lung cancer (NSCLC), its functions in the progression of NSCLC and the related molecular mechanism were not fully understood. In this study,...
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| Veröffentlicht in: | Tumor biology 2016-08, Vol.37 (8), p.10909-10915 |
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| creator | Liang, Zhigang Wang, Ying Shen, Zhenya Teng, Xiaomei Li, Xinjian Li, Chenwei Wu, Weijie Zhou, Zenghui Wang, Zishan |
| description | Fascin 1 (Fascin actin-bundling protein 1) is an actin-binding protein. Although several studies have reported the dysregulation of Fascin 1 in non-small cell lung cancer (NSCLC), its functions in the progression of NSCLC and the related molecular mechanism were not fully understood. In this study, the expression of Fascin 1 in NSCLC tissues was determined using quantitative PCR (qPCR), and the roles of Fascin 1 in the progression of NSCLC were investigated. It was found that both the messenger RNA (mRNA) level and the protein level of Fascin 1 were upregulated in NSCLC tissues. Forced expression of Fascin 1 promoted the growth and migration of NSCLC cells, while knocking down the expression of Fascin 1 inhibited the growth, migration, and tumorigenesis of NSCLC cells. Mechanism studies showed that Fascin 1 increased the transcriptional activity of the YAP/TEAD (Yes-associated protein/TEA domain transcriptional factor) complex, and knocking down the expression of Fascin 1 attenuated the expression of target genes downstream the YAP/TEAD complex. In addition, MST1 interacted with Fascin 1. Taken together, Fascin 1 plays an oncogenic role in NSCLC by activating the transcriptional activity of the YAP/TEAD complex. |
| doi_str_mv | 10.1007/s13277-016-4934-0 |
| format | Article |
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Although several studies have reported the dysregulation of Fascin 1 in non-small cell lung cancer (NSCLC), its functions in the progression of NSCLC and the related molecular mechanism were not fully understood. In this study, the expression of Fascin 1 in NSCLC tissues was determined using quantitative PCR (qPCR), and the roles of Fascin 1 in the progression of NSCLC were investigated. It was found that both the messenger RNA (mRNA) level and the protein level of Fascin 1 were upregulated in NSCLC tissues. Forced expression of Fascin 1 promoted the growth and migration of NSCLC cells, while knocking down the expression of Fascin 1 inhibited the growth, migration, and tumorigenesis of NSCLC cells. Mechanism studies showed that Fascin 1 increased the transcriptional activity of the YAP/TEAD (Yes-associated protein/TEA domain transcriptional factor) complex, and knocking down the expression of Fascin 1 attenuated the expression of target genes downstream the YAP/TEAD complex. In addition, MST1 interacted with Fascin 1. Taken together, Fascin 1 plays an oncogenic role in NSCLC by activating the transcriptional activity of the YAP/TEAD complex.</description><identifier>ISSN: 1010-4283</identifier><identifier>EISSN: 1423-0380</identifier><identifier>DOI: 10.1007/s13277-016-4934-0</identifier><identifier>PMID: 26886283</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adaptor Proteins, Signal Transducing - metabolism ; Biomedical and Life Sciences ; Biomedicine ; Blotting, Western ; Cancer Research ; Carcinoma, Non-Small-Cell Lung - metabolism ; Carcinoma, Non-Small-Cell Lung - pathology ; Carrier Proteins - metabolism ; Cell adhesion & migration ; Cell growth ; Cell Line, Tumor ; Cell Movement - physiology ; Cell Proliferation - physiology ; DNA-Binding Proteins - metabolism ; Gene Expression Regulation, Neoplastic - physiology ; Gene Knockdown Techniques ; Humans ; Immunohistochemistry ; Immunoprecipitation ; Lung cancer ; Lung Neoplasms - metabolism ; Lung Neoplasms - pathology ; Microfilament Proteins - metabolism ; Nuclear Proteins - metabolism ; Original Article ; Phosphoproteins - metabolism ; Real-Time Polymerase Chain Reaction ; Signal Transduction - physiology ; Studies ; Transcription Factors - metabolism</subject><ispartof>Tumor biology, 2016-08, Vol.37 (8), p.10909-10915</ispartof><rights>International Society of Oncology and BioMarkers (ISOBM) 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-c134ab52da9bdef00f63c0878f0b3e4cf9fa599406e5f82462bbc45b2b1c0de23</citedby><cites>FETCH-LOGICAL-c372t-c134ab52da9bdef00f63c0878f0b3e4cf9fa599406e5f82462bbc45b2b1c0de23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13277-016-4934-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13277-016-4934-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26886283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Zhigang</creatorcontrib><creatorcontrib>Wang, Ying</creatorcontrib><creatorcontrib>Shen, Zhenya</creatorcontrib><creatorcontrib>Teng, Xiaomei</creatorcontrib><creatorcontrib>Li, Xinjian</creatorcontrib><creatorcontrib>Li, Chenwei</creatorcontrib><creatorcontrib>Wu, Weijie</creatorcontrib><creatorcontrib>Zhou, Zenghui</creatorcontrib><creatorcontrib>Wang, Zishan</creatorcontrib><title>Fascin 1 promoted the growth and migration of non-small cell lung cancer cells by activating YAP/TEAD signaling</title><title>Tumor biology</title><addtitle>Tumor Biol</addtitle><addtitle>Tumour Biol</addtitle><description>Fascin 1 (Fascin actin-bundling protein 1) is an actin-binding protein. Although several studies have reported the dysregulation of Fascin 1 in non-small cell lung cancer (NSCLC), its functions in the progression of NSCLC and the related molecular mechanism were not fully understood. In this study, the expression of Fascin 1 in NSCLC tissues was determined using quantitative PCR (qPCR), and the roles of Fascin 1 in the progression of NSCLC were investigated. It was found that both the messenger RNA (mRNA) level and the protein level of Fascin 1 were upregulated in NSCLC tissues. Forced expression of Fascin 1 promoted the growth and migration of NSCLC cells, while knocking down the expression of Fascin 1 inhibited the growth, migration, and tumorigenesis of NSCLC cells. Mechanism studies showed that Fascin 1 increased the transcriptional activity of the YAP/TEAD (Yes-associated protein/TEA domain transcriptional factor) complex, and knocking down the expression of Fascin 1 attenuated the expression of target genes downstream the YAP/TEAD complex. In addition, MST1 interacted with Fascin 1. Taken together, Fascin 1 plays an oncogenic role in NSCLC by activating the transcriptional activity of the YAP/TEAD complex.</description><subject>Adaptor Proteins, Signal Transducing - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blotting, Western</subject><subject>Cancer Research</subject><subject>Carcinoma, Non-Small-Cell Lung - metabolism</subject><subject>Carcinoma, Non-Small-Cell Lung - pathology</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell adhesion & migration</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - physiology</subject><subject>Cell Proliferation - physiology</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Gene Expression Regulation, Neoplastic - physiology</subject><subject>Gene Knockdown Techniques</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Immunoprecipitation</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - pathology</subject><subject>Microfilament Proteins - metabolism</subject><subject>Nuclear Proteins - metabolism</subject><subject>Original Article</subject><subject>Phosphoproteins - metabolism</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Signal Transduction - physiology</subject><subject>Studies</subject><subject>Transcription Factors - metabolism</subject><issn>1010-4283</issn><issn>1423-0380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctO3TAQhq0KVK4PwKayxIaNy_hyEmd5RIFWQioLuujKsh07BCU2tZMi3h6H0ApV6sa2Zr75Z8Y_QicUPlOA-jxTzuqaAK2IaLgg8AHtU8E4AS5hp7yBAhFM8j10kPMDAN00TfUR7bFKyqrE91G80tn2AVP8mOIYJ9fi6d7hLsWn6R7r0OKx75Ke-hhw9DjEQPKohwFbV45hDh22OliXXgMZm2es7dT_LhUl9XN7e353uf2Cc98FPZTQEdr1esju-O0-RD-uLu8uvpKb79ffLrY3xPKaTcRSLrTZsFY3pnUewFfcgqylB8OdsL7xuuwioHIbL5momDFWbAwz1ELrGD9EZ6tuWevX7PKkxj4vI-rg4pwVlVRUknJY0NN_0Ic4pzLuSkEtqFwoulI2xZyT8-ox9aNOz4qCWtxQqxuquKEWNxSUmk9vyrMZXfu34s_3F4CtQC6p0Ln0rvV_VV8AzMOUZQ</recordid><startdate>20160801</startdate><enddate>20160801</enddate><creator>Liang, Zhigang</creator><creator>Wang, Ying</creator><creator>Shen, Zhenya</creator><creator>Teng, Xiaomei</creator><creator>Li, Xinjian</creator><creator>Li, Chenwei</creator><creator>Wu, Weijie</creator><creator>Zhou, Zenghui</creator><creator>Wang, Zishan</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TO</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20160801</creationdate><title>Fascin 1 promoted the growth and migration of non-small cell lung cancer cells by activating YAP/TEAD signaling</title><author>Liang, Zhigang ; Wang, Ying ; Shen, Zhenya ; Teng, Xiaomei ; Li, Xinjian ; Li, Chenwei ; Wu, Weijie ; Zhou, Zenghui ; Wang, Zishan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-c134ab52da9bdef00f63c0878f0b3e4cf9fa599406e5f82462bbc45b2b1c0de23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adaptor Proteins, Signal Transducing - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blotting, Western</topic><topic>Cancer Research</topic><topic>Carcinoma, Non-Small-Cell Lung - metabolism</topic><topic>Carcinoma, Non-Small-Cell Lung - pathology</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell adhesion & migration</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - physiology</topic><topic>Cell Proliferation - physiology</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Gene Expression Regulation, Neoplastic - physiology</topic><topic>Gene Knockdown Techniques</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Immunoprecipitation</topic><topic>Lung cancer</topic><topic>Lung Neoplasms - metabolism</topic><topic>Lung Neoplasms - pathology</topic><topic>Microfilament Proteins - metabolism</topic><topic>Nuclear Proteins - metabolism</topic><topic>Original Article</topic><topic>Phosphoproteins - metabolism</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Signal Transduction - physiology</topic><topic>Studies</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liang, Zhigang</creatorcontrib><creatorcontrib>Wang, Ying</creatorcontrib><creatorcontrib>Shen, Zhenya</creatorcontrib><creatorcontrib>Teng, Xiaomei</creatorcontrib><creatorcontrib>Li, Xinjian</creatorcontrib><creatorcontrib>Li, Chenwei</creatorcontrib><creatorcontrib>Wu, Weijie</creatorcontrib><creatorcontrib>Zhou, Zenghui</creatorcontrib><creatorcontrib>Wang, Zishan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Tumor biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Zhigang</au><au>Wang, Ying</au><au>Shen, Zhenya</au><au>Teng, Xiaomei</au><au>Li, Xinjian</au><au>Li, Chenwei</au><au>Wu, Weijie</au><au>Zhou, Zenghui</au><au>Wang, Zishan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fascin 1 promoted the growth and migration of non-small cell lung cancer cells by activating YAP/TEAD signaling</atitle><jtitle>Tumor biology</jtitle><stitle>Tumor Biol</stitle><addtitle>Tumour Biol</addtitle><date>2016-08-01</date><risdate>2016</risdate><volume>37</volume><issue>8</issue><spage>10909</spage><epage>10915</epage><pages>10909-10915</pages><issn>1010-4283</issn><eissn>1423-0380</eissn><abstract>Fascin 1 (Fascin actin-bundling protein 1) is an actin-binding protein. Although several studies have reported the dysregulation of Fascin 1 in non-small cell lung cancer (NSCLC), its functions in the progression of NSCLC and the related molecular mechanism were not fully understood. In this study, the expression of Fascin 1 in NSCLC tissues was determined using quantitative PCR (qPCR), and the roles of Fascin 1 in the progression of NSCLC were investigated. It was found that both the messenger RNA (mRNA) level and the protein level of Fascin 1 were upregulated in NSCLC tissues. Forced expression of Fascin 1 promoted the growth and migration of NSCLC cells, while knocking down the expression of Fascin 1 inhibited the growth, migration, and tumorigenesis of NSCLC cells. Mechanism studies showed that Fascin 1 increased the transcriptional activity of the YAP/TEAD (Yes-associated protein/TEA domain transcriptional factor) complex, and knocking down the expression of Fascin 1 attenuated the expression of target genes downstream the YAP/TEAD complex. In addition, MST1 interacted with Fascin 1. Taken together, Fascin 1 plays an oncogenic role in NSCLC by activating the transcriptional activity of the YAP/TEAD complex.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>26886283</pmid><doi>10.1007/s13277-016-4934-0</doi><tpages>7</tpages></addata></record> |
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| subjects | Adaptor Proteins, Signal Transducing - metabolism Biomedical and Life Sciences Biomedicine Blotting, Western Cancer Research Carcinoma, Non-Small-Cell Lung - metabolism Carcinoma, Non-Small-Cell Lung - pathology Carrier Proteins - metabolism Cell adhesion & migration Cell growth Cell Line, Tumor Cell Movement - physiology Cell Proliferation - physiology DNA-Binding Proteins - metabolism Gene Expression Regulation, Neoplastic - physiology Gene Knockdown Techniques Humans Immunohistochemistry Immunoprecipitation Lung cancer Lung Neoplasms - metabolism Lung Neoplasms - pathology Microfilament Proteins - metabolism Nuclear Proteins - metabolism Original Article Phosphoproteins - metabolism Real-Time Polymerase Chain Reaction Signal Transduction - physiology Studies Transcription Factors - metabolism |
| title | Fascin 1 promoted the growth and migration of non-small cell lung cancer cells by activating YAP/TEAD signaling |
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