Unravelling the in vivo regulation and metabolic role of the alternative oxidase pathway in C3 species under photoinhibitory conditions
The mitochondrial alternative oxidase pathway (AOP) has been suggested to act as a sink for excess reducing power generated in the chloroplast under high-light (HL) stress and thus may reduce photoinhibition. The aim of this study was to compare different species to investigate the in vivo regulatio...
Gespeichert in:
| Veröffentlicht in: | The New phytologist 2016-10, Vol.212 (1), p.66-79 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 79 |
|---|---|
| container_issue | 1 |
| container_start_page | 66 |
| container_title | The New phytologist |
| container_volume | 212 |
| creator | Florez‐Sarasa, Igor Ribas‐Carbo, Miquel Del‐Saz, Néstor Fernández Schwahn, Kevin Nikoloski, Zoran Fernie, Alisdair R. Flexas, Jaume |
| description | The mitochondrial alternative oxidase pathway (AOP) has been suggested to act as a sink for excess reducing power generated in the chloroplast under high-light (HL) stress and thus may reduce photoinhibition. The aim of this study was to compare different species to investigate the in vivo regulation and role of AOP under HL stress.
The in vivo activities of AOP (malt) and the cytochrome oxidase pathway, chlorophyll fluorescence, metabolite profiles, alternative oxidase (AOX) capacity and protein amount were determined in leaves of five C3 species under growth light and after HL treatment.
Differences in respiration and metabolite levels were observed among species under growth light conditions. The HL response of malt was highly species dependent, correlated with the AOP capacity and independent of AOX protein content. Nevertheless, significant correlations were observed between malt, levels of key metabolites and photosynthetic parameters.
The results show that the species-specific response of malt is caused by the differential posttranslational regulation of AOX. Significant correlations between respiration, metabolites and photosynthetic performance across species suggest that AOP may permit stress-related amino acid synthesis, whilst maintaining photosynthetic activity under HL stress. |
| doi_str_mv | 10.1111/nph.14030 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1814655724</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>newphytologist.212.1.66</jstor_id><sourcerecordid>newphytologist.212.1.66</sourcerecordid><originalsourceid>FETCH-LOGICAL-j3010-60ec61bea018321600db64f84b24c3228456114ba01ed226e0a3976d27e58b5d3</originalsourceid><addsrcrecordid>eNpdkc1uEzEUhS0EoqGw4AWQJTYsmNZ_43GWKIIWqQIWVGJnecY3GUeOPdgzCfMEfW2cpHSBN7Z8v3vtcw5Cbym5omVdh6G_ooJw8gwtqJDLSlHePEcLQpiqpJC_LtCrnLeEkGUt2Ut0wRrOKCNqgR7uQzJ78N6FDR57wC7gvdtHnGAzeTO6GLAJFu9gNG30rsMpesBxfYKNHyGFQu3L1R9nTQY8mLE_mPk4aMVxHqBzkPEULCQ89HGMLvSudWNMM-5isO74Rn6NXqyNz_Dmcb9E918-_1zdVnffb76uPt1VW04oqSSBTtIWDKGqSJCE2FaKtRItEx1nTIlaUiraUgfLmARi-LKRljVQq7a2_BJ9OM8dUvw9QR71zuWu6DcB4pQ1VcXAum6YKOj7_9BtnIpaf6K4ahihdaHePVJTuwOrh-R2Js36n8UFuD4DB-dhfqpToo_Z6ZKdPmWnv_24PR1Kx8dzxzYXl546AhyGfh6jjxtXPlOma6ql5H8BA8Scpg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1813872015</pqid></control><display><type>article</type><title>Unravelling the in vivo regulation and metabolic role of the alternative oxidase pathway in C3 species under photoinhibitory conditions</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Jstor Complete Legacy</source><source>Wiley Free Content</source><creator>Florez‐Sarasa, Igor ; Ribas‐Carbo, Miquel ; Del‐Saz, Néstor Fernández ; Schwahn, Kevin ; Nikoloski, Zoran ; Fernie, Alisdair R. ; Flexas, Jaume</creator><creatorcontrib>Florez‐Sarasa, Igor ; Ribas‐Carbo, Miquel ; Del‐Saz, Néstor Fernández ; Schwahn, Kevin ; Nikoloski, Zoran ; Fernie, Alisdair R. ; Flexas, Jaume</creatorcontrib><description>The mitochondrial alternative oxidase pathway (AOP) has been suggested to act as a sink for excess reducing power generated in the chloroplast under high-light (HL) stress and thus may reduce photoinhibition. The aim of this study was to compare different species to investigate the in vivo regulation and role of AOP under HL stress.
The in vivo activities of AOP (malt) and the cytochrome oxidase pathway, chlorophyll fluorescence, metabolite profiles, alternative oxidase (AOX) capacity and protein amount were determined in leaves of five C3 species under growth light and after HL treatment.
Differences in respiration and metabolite levels were observed among species under growth light conditions. The HL response of malt was highly species dependent, correlated with the AOP capacity and independent of AOX protein content. Nevertheless, significant correlations were observed between malt, levels of key metabolites and photosynthetic parameters.
The results show that the species-specific response of malt is caused by the differential posttranslational regulation of AOX. Significant correlations between respiration, metabolites and photosynthetic performance across species suggest that AOP may permit stress-related amino acid synthesis, whilst maintaining photosynthetic activity under HL stress.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.14030</identifier><identifier>PMID: 27321208</identifier><language>eng</language><publisher>England: New Phytologist Trust</publisher><subject>alternative oxidase (AOX) ; C3 species ; Carbon - metabolism ; Cell Respiration - radiation effects ; Chlorophyll - metabolism ; Electron Transport - radiation effects ; Electron Transport Complex IV - metabolism ; Fluorescence ; high light ; Light ; metabolite profiling ; Metabolome - radiation effects ; Metabolomics ; Mitochondrial Proteins - metabolism ; Oxidoreductases - metabolism ; oxygen isotope fractionation ; photoinhibition ; Photosynthesis - radiation effects ; Photosystem II Protein Complex - metabolism ; Plant Leaves - metabolism ; Plant Leaves - radiation effects ; Plant Proteins - metabolism ; Plants - metabolism ; Plants - radiation effects ; Species Specificity</subject><ispartof>The New phytologist, 2016-10, Vol.212 (1), p.66-79</ispartof><rights>2016 New Phytologist Trust</rights><rights>2016 The Authors. New Phytologist © 2016 New Phytologist Trust</rights><rights>2016 The Authors. New Phytologist © 2016 New Phytologist Trust.</rights><rights>Copyright © 2016 New Phytologist Trust</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/newphytologist.212.1.66$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/newphytologist.212.1.66$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,1411,1427,27903,27904,45553,45554,46388,46812,57996,58229</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27321208$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Florez‐Sarasa, Igor</creatorcontrib><creatorcontrib>Ribas‐Carbo, Miquel</creatorcontrib><creatorcontrib>Del‐Saz, Néstor Fernández</creatorcontrib><creatorcontrib>Schwahn, Kevin</creatorcontrib><creatorcontrib>Nikoloski, Zoran</creatorcontrib><creatorcontrib>Fernie, Alisdair R.</creatorcontrib><creatorcontrib>Flexas, Jaume</creatorcontrib><title>Unravelling the in vivo regulation and metabolic role of the alternative oxidase pathway in C3 species under photoinhibitory conditions</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>The mitochondrial alternative oxidase pathway (AOP) has been suggested to act as a sink for excess reducing power generated in the chloroplast under high-light (HL) stress and thus may reduce photoinhibition. The aim of this study was to compare different species to investigate the in vivo regulation and role of AOP under HL stress.
The in vivo activities of AOP (malt) and the cytochrome oxidase pathway, chlorophyll fluorescence, metabolite profiles, alternative oxidase (AOX) capacity and protein amount were determined in leaves of five C3 species under growth light and after HL treatment.
Differences in respiration and metabolite levels were observed among species under growth light conditions. The HL response of malt was highly species dependent, correlated with the AOP capacity and independent of AOX protein content. Nevertheless, significant correlations were observed between malt, levels of key metabolites and photosynthetic parameters.
The results show that the species-specific response of malt is caused by the differential posttranslational regulation of AOX. Significant correlations between respiration, metabolites and photosynthetic performance across species suggest that AOP may permit stress-related amino acid synthesis, whilst maintaining photosynthetic activity under HL stress.</description><subject>alternative oxidase (AOX)</subject><subject>C3 species</subject><subject>Carbon - metabolism</subject><subject>Cell Respiration - radiation effects</subject><subject>Chlorophyll - metabolism</subject><subject>Electron Transport - radiation effects</subject><subject>Electron Transport Complex IV - metabolism</subject><subject>Fluorescence</subject><subject>high light</subject><subject>Light</subject><subject>metabolite profiling</subject><subject>Metabolome - radiation effects</subject><subject>Metabolomics</subject><subject>Mitochondrial Proteins - metabolism</subject><subject>Oxidoreductases - metabolism</subject><subject>oxygen isotope fractionation</subject><subject>photoinhibition</subject><subject>Photosynthesis - radiation effects</subject><subject>Photosystem II Protein Complex - metabolism</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Leaves - radiation effects</subject><subject>Plant Proteins - metabolism</subject><subject>Plants - metabolism</subject><subject>Plants - radiation effects</subject><subject>Species Specificity</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1uEzEUhS0EoqGw4AWQJTYsmNZ_43GWKIIWqQIWVGJnecY3GUeOPdgzCfMEfW2cpHSBN7Z8v3vtcw5Cbym5omVdh6G_ooJw8gwtqJDLSlHePEcLQpiqpJC_LtCrnLeEkGUt2Ut0wRrOKCNqgR7uQzJ78N6FDR57wC7gvdtHnGAzeTO6GLAJFu9gNG30rsMpesBxfYKNHyGFQu3L1R9nTQY8mLE_mPk4aMVxHqBzkPEULCQ89HGMLvSudWNMM-5isO74Rn6NXqyNz_Dmcb9E918-_1zdVnffb76uPt1VW04oqSSBTtIWDKGqSJCE2FaKtRItEx1nTIlaUiraUgfLmARi-LKRljVQq7a2_BJ9OM8dUvw9QR71zuWu6DcB4pQ1VcXAum6YKOj7_9BtnIpaf6K4ahihdaHePVJTuwOrh-R2Js36n8UFuD4DB-dhfqpToo_Z6ZKdPmWnv_24PR1Kx8dzxzYXl546AhyGfh6jjxtXPlOma6ql5H8BA8Scpg</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Florez‐Sarasa, Igor</creator><creator>Ribas‐Carbo, Miquel</creator><creator>Del‐Saz, Néstor Fernández</creator><creator>Schwahn, Kevin</creator><creator>Nikoloski, Zoran</creator><creator>Fernie, Alisdair R.</creator><creator>Flexas, Jaume</creator><general>New Phytologist Trust</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201610</creationdate><title>Unravelling the in vivo regulation and metabolic role of the alternative oxidase pathway in C3 species under photoinhibitory conditions</title><author>Florez‐Sarasa, Igor ; Ribas‐Carbo, Miquel ; Del‐Saz, Néstor Fernández ; Schwahn, Kevin ; Nikoloski, Zoran ; Fernie, Alisdair R. ; Flexas, Jaume</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j3010-60ec61bea018321600db64f84b24c3228456114ba01ed226e0a3976d27e58b5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>alternative oxidase (AOX)</topic><topic>C3 species</topic><topic>Carbon - metabolism</topic><topic>Cell Respiration - radiation effects</topic><topic>Chlorophyll - metabolism</topic><topic>Electron Transport - radiation effects</topic><topic>Electron Transport Complex IV - metabolism</topic><topic>Fluorescence</topic><topic>high light</topic><topic>Light</topic><topic>metabolite profiling</topic><topic>Metabolome - radiation effects</topic><topic>Metabolomics</topic><topic>Mitochondrial Proteins - metabolism</topic><topic>Oxidoreductases - metabolism</topic><topic>oxygen isotope fractionation</topic><topic>photoinhibition</topic><topic>Photosynthesis - radiation effects</topic><topic>Photosystem II Protein Complex - metabolism</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Leaves - radiation effects</topic><topic>Plant Proteins - metabolism</topic><topic>Plants - metabolism</topic><topic>Plants - radiation effects</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Florez‐Sarasa, Igor</creatorcontrib><creatorcontrib>Ribas‐Carbo, Miquel</creatorcontrib><creatorcontrib>Del‐Saz, Néstor Fernández</creatorcontrib><creatorcontrib>Schwahn, Kevin</creatorcontrib><creatorcontrib>Nikoloski, Zoran</creatorcontrib><creatorcontrib>Fernie, Alisdair R.</creatorcontrib><creatorcontrib>Flexas, Jaume</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Florez‐Sarasa, Igor</au><au>Ribas‐Carbo, Miquel</au><au>Del‐Saz, Néstor Fernández</au><au>Schwahn, Kevin</au><au>Nikoloski, Zoran</au><au>Fernie, Alisdair R.</au><au>Flexas, Jaume</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Unravelling the in vivo regulation and metabolic role of the alternative oxidase pathway in C3 species under photoinhibitory conditions</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2016-10</date><risdate>2016</risdate><volume>212</volume><issue>1</issue><spage>66</spage><epage>79</epage><pages>66-79</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>The mitochondrial alternative oxidase pathway (AOP) has been suggested to act as a sink for excess reducing power generated in the chloroplast under high-light (HL) stress and thus may reduce photoinhibition. The aim of this study was to compare different species to investigate the in vivo regulation and role of AOP under HL stress.
The in vivo activities of AOP (malt) and the cytochrome oxidase pathway, chlorophyll fluorescence, metabolite profiles, alternative oxidase (AOX) capacity and protein amount were determined in leaves of five C3 species under growth light and after HL treatment.
Differences in respiration and metabolite levels were observed among species under growth light conditions. The HL response of malt was highly species dependent, correlated with the AOP capacity and independent of AOX protein content. Nevertheless, significant correlations were observed between malt, levels of key metabolites and photosynthetic parameters.
The results show that the species-specific response of malt is caused by the differential posttranslational regulation of AOX. Significant correlations between respiration, metabolites and photosynthetic performance across species suggest that AOP may permit stress-related amino acid synthesis, whilst maintaining photosynthetic activity under HL stress.</abstract><cop>England</cop><pub>New Phytologist Trust</pub><pmid>27321208</pmid><doi>10.1111/nph.14030</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-646X |
| ispartof | The New phytologist, 2016-10, Vol.212 (1), p.66-79 |
| issn | 0028-646X 1469-8137 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1814655724 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Jstor Complete Legacy; Wiley Free Content |
| subjects | alternative oxidase (AOX) C3 species Carbon - metabolism Cell Respiration - radiation effects Chlorophyll - metabolism Electron Transport - radiation effects Electron Transport Complex IV - metabolism Fluorescence high light Light metabolite profiling Metabolome - radiation effects Metabolomics Mitochondrial Proteins - metabolism Oxidoreductases - metabolism oxygen isotope fractionation photoinhibition Photosynthesis - radiation effects Photosystem II Protein Complex - metabolism Plant Leaves - metabolism Plant Leaves - radiation effects Plant Proteins - metabolism Plants - metabolism Plants - radiation effects Species Specificity |
| title | Unravelling the in vivo regulation and metabolic role of the alternative oxidase pathway in C3 species under photoinhibitory conditions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T17%3A36%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Unravelling%20the%20in%20vivo%20regulation%20and%20metabolic%20role%20of%20the%20alternative%20oxidase%20pathway%20in%20C3%20species%20under%20photoinhibitory%20conditions&rft.jtitle=The%20New%20phytologist&rft.au=Florez%E2%80%90Sarasa,%20Igor&rft.date=2016-10&rft.volume=212&rft.issue=1&rft.spage=66&rft.epage=79&rft.pages=66-79&rft.issn=0028-646X&rft.eissn=1469-8137&rft_id=info:doi/10.1111/nph.14030&rft_dat=%3Cjstor_proqu%3Enewphytologist.212.1.66%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1813872015&rft_id=info:pmid/27321208&rft_jstor_id=newphytologist.212.1.66&rfr_iscdi=true |