Ex vivo detection of tumoral lymph nodes of colorectal origin with fluorescence imaging after intraoperative intravenous injection of indocyanine green
Background and Objectives The aim of this study was to investigate the potential role of indocyanine green (ICG) fluorescence imaging after intraoperative intravenous (IV) injection for the “ex vivo” detection of metastatic lymph nodes (mLNs) of colorectal cancer origin. Methods Fresh‐fixed LNs in c...
Gespeichert in:
| Veröffentlicht in: | Journal of surgical oncology 2016-09, Vol.114 (3), p.348-353 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 353 |
|---|---|
| container_issue | 3 |
| container_start_page | 348 |
| container_title | Journal of surgical oncology |
| container_volume | 114 |
| creator | Liberale, Gabriel Galdon, Maria Gomez Moreau, Michel Vankerckhove, Sophie El Nakadi, Issam Larsimont, Denis Donckier, Vincent Bourgeois, Pierre |
| description | Background and Objectives
The aim of this study was to investigate the potential role of indocyanine green (ICG) fluorescence imaging after intraoperative intravenous (IV) injection for the “ex vivo” detection of metastatic lymph nodes (mLNs) of colorectal cancer origin.
Methods
Fresh‐fixed LNs in cassettes and/or paraffin‐embedded LNs of patients included in a study that evaluated the role of ICG in the detection of peritoneal metastases of colorectal origin (Protocol NCT‐01995591) were further explored with a dedicated near‐infrared camera system for their fluorescence. An IV injection of ICG was delivered intraoperatively at 0.25 mg/kg. Signal to background ratios (SBRs) were calculated.
Results
LNs on operative specimens were evaluated for 12 patients (5 males, 7 females). A total of 182 LNs were analyzed. The mean LN number per patient was 15.2 (median: 15.5; range 3–22). SBRs of mLNs were significantly more fluorescent than benign LNs, 1.41 versus 1.04 arbitrary units (P 20 mm2) (P |
| doi_str_mv | 10.1002/jso.24318 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1813900245</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1813900245</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4618-21957ae61d669e005f1ec6308044fe174592c0815fb9705dd344e68d1047222e3</originalsourceid><addsrcrecordid>eNp1kcFu1DAQhi0EokvhwAsgS1zgkHbs2E58pFVpqSp6oKiIi-Umk62XrL21k233Sfq6eElbJCROtme--Wc8PyFvGewxAL6_SGGPi5LVz8iMgVaFBl0_J7Oc44WoNOyQVyktAEBrJV6SHV5xJTjAjNwf3dG1Wwfa4oDN4IKnoaPDuAzR9rTfLFfX1IcW0zbchD7ETOVMiG7uPL11wzXt-jGHU4O-QeqWNifm1HYDRur8EG1YYbSDW-P0XKMPY8r3xd-Gzreh2VjvPNJ5RPSvyYvO9gnfPJy75Pvno4vDk-Ls_PjL4aezohGK1QVnWlYWFWuV0gggO4aNKqEGITpklZCaN1Az2V3pCmTblkKgqlsGouKcY7lLPky6qxhuRkyDWbr8k763HvOUhtWs1HmPQmb0_T_oIozR5-m2FK8rqaXK1MeJamJIKWJnVjHvJG4MA7N1y2S3zB-3MvvuQXG8WmL7RD7ak4H9Cbh1PW7-r2ROv50_ShZThUsD3j1V2PjLqKqspLn8emwO5M-LH6fs0hyUvwEWma-2</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1812875956</pqid></control><display><type>article</type><title>Ex vivo detection of tumoral lymph nodes of colorectal origin with fluorescence imaging after intraoperative intravenous injection of indocyanine green</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Liberale, Gabriel ; Galdon, Maria Gomez ; Moreau, Michel ; Vankerckhove, Sophie ; El Nakadi, Issam ; Larsimont, Denis ; Donckier, Vincent ; Bourgeois, Pierre</creator><creatorcontrib>Liberale, Gabriel ; Galdon, Maria Gomez ; Moreau, Michel ; Vankerckhove, Sophie ; El Nakadi, Issam ; Larsimont, Denis ; Donckier, Vincent ; Bourgeois, Pierre</creatorcontrib><description>Background and Objectives
The aim of this study was to investigate the potential role of indocyanine green (ICG) fluorescence imaging after intraoperative intravenous (IV) injection for the “ex vivo” detection of metastatic lymph nodes (mLNs) of colorectal cancer origin.
Methods
Fresh‐fixed LNs in cassettes and/or paraffin‐embedded LNs of patients included in a study that evaluated the role of ICG in the detection of peritoneal metastases of colorectal origin (Protocol NCT‐01995591) were further explored with a dedicated near‐infrared camera system for their fluorescence. An IV injection of ICG was delivered intraoperatively at 0.25 mg/kg. Signal to background ratios (SBRs) were calculated.
Results
LNs on operative specimens were evaluated for 12 patients (5 males, 7 females). A total of 182 LNs were analyzed. The mean LN number per patient was 15.2 (median: 15.5; range 3–22). SBRs of mLNs were significantly more fluorescent than benign LNs, 1.41 versus 1.04 arbitrary units (P < 0.0002). On univariate analysis, fluorescence was statistically correlated with LN surface area (>20 mm2) (P < 0.0004).
Conclusion
Ex vivo ICG fluorescence imaging after intraoperative IV injection represents a potential method for detecting invaded LN's of colorectal cancer origin on operative specimens. Further clinical studies are needed to better define optimal techniques. J. Surg. Oncol. 2016;114:348–353. © 2016 Wiley Periodicals, Inc.</description><identifier>ISSN: 0022-4790</identifier><identifier>EISSN: 1096-9098</identifier><identifier>DOI: 10.1002/jso.24318</identifier><identifier>PMID: 27264200</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Aged ; colorectal neoplasms ; Colorectal Neoplasms - pathology ; Colorectal Neoplasms - surgery ; ex vivo ; Female ; fluorescence imaging ; Fluorescent Dyes ; Humans ; Indocyanine Green ; Lymph Node Excision ; lymph nodes ; Lymph Nodes - diagnostic imaging ; Male ; metastatic ; Middle Aged ; Optical Imaging ; Peritoneal Neoplasms - diagnostic imaging ; Peritoneal Neoplasms - secondary ; Peritoneal Neoplasms - surgery ; Sensitivity and Specificity</subject><ispartof>Journal of surgical oncology, 2016-09, Vol.114 (3), p.348-353</ispartof><rights>2016 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4618-21957ae61d669e005f1ec6308044fe174592c0815fb9705dd344e68d1047222e3</citedby><cites>FETCH-LOGICAL-c4618-21957ae61d669e005f1ec6308044fe174592c0815fb9705dd344e68d1047222e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjso.24318$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjso.24318$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27264200$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liberale, Gabriel</creatorcontrib><creatorcontrib>Galdon, Maria Gomez</creatorcontrib><creatorcontrib>Moreau, Michel</creatorcontrib><creatorcontrib>Vankerckhove, Sophie</creatorcontrib><creatorcontrib>El Nakadi, Issam</creatorcontrib><creatorcontrib>Larsimont, Denis</creatorcontrib><creatorcontrib>Donckier, Vincent</creatorcontrib><creatorcontrib>Bourgeois, Pierre</creatorcontrib><title>Ex vivo detection of tumoral lymph nodes of colorectal origin with fluorescence imaging after intraoperative intravenous injection of indocyanine green</title><title>Journal of surgical oncology</title><addtitle>J. Surg. Oncol</addtitle><description>Background and Objectives
The aim of this study was to investigate the potential role of indocyanine green (ICG) fluorescence imaging after intraoperative intravenous (IV) injection for the “ex vivo” detection of metastatic lymph nodes (mLNs) of colorectal cancer origin.
Methods
Fresh‐fixed LNs in cassettes and/or paraffin‐embedded LNs of patients included in a study that evaluated the role of ICG in the detection of peritoneal metastases of colorectal origin (Protocol NCT‐01995591) were further explored with a dedicated near‐infrared camera system for their fluorescence. An IV injection of ICG was delivered intraoperatively at 0.25 mg/kg. Signal to background ratios (SBRs) were calculated.
Results
LNs on operative specimens were evaluated for 12 patients (5 males, 7 females). A total of 182 LNs were analyzed. The mean LN number per patient was 15.2 (median: 15.5; range 3–22). SBRs of mLNs were significantly more fluorescent than benign LNs, 1.41 versus 1.04 arbitrary units (P < 0.0002). On univariate analysis, fluorescence was statistically correlated with LN surface area (>20 mm2) (P < 0.0004).
Conclusion
Ex vivo ICG fluorescence imaging after intraoperative IV injection represents a potential method for detecting invaded LN's of colorectal cancer origin on operative specimens. Further clinical studies are needed to better define optimal techniques. J. Surg. Oncol. 2016;114:348–353. © 2016 Wiley Periodicals, Inc.</description><subject>Aged</subject><subject>colorectal neoplasms</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Colorectal Neoplasms - surgery</subject><subject>ex vivo</subject><subject>Female</subject><subject>fluorescence imaging</subject><subject>Fluorescent Dyes</subject><subject>Humans</subject><subject>Indocyanine Green</subject><subject>Lymph Node Excision</subject><subject>lymph nodes</subject><subject>Lymph Nodes - diagnostic imaging</subject><subject>Male</subject><subject>metastatic</subject><subject>Middle Aged</subject><subject>Optical Imaging</subject><subject>Peritoneal Neoplasms - diagnostic imaging</subject><subject>Peritoneal Neoplasms - secondary</subject><subject>Peritoneal Neoplasms - surgery</subject><subject>Sensitivity and Specificity</subject><issn>0022-4790</issn><issn>1096-9098</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFu1DAQhi0EokvhwAsgS1zgkHbs2E58pFVpqSp6oKiIi-Umk62XrL21k233Sfq6eElbJCROtme--Wc8PyFvGewxAL6_SGGPi5LVz8iMgVaFBl0_J7Oc44WoNOyQVyktAEBrJV6SHV5xJTjAjNwf3dG1Wwfa4oDN4IKnoaPDuAzR9rTfLFfX1IcW0zbchD7ETOVMiG7uPL11wzXt-jGHU4O-QeqWNifm1HYDRur8EG1YYbSDW-P0XKMPY8r3xd-Gzreh2VjvPNJ5RPSvyYvO9gnfPJy75Pvno4vDk-Ls_PjL4aezohGK1QVnWlYWFWuV0gggO4aNKqEGITpklZCaN1Az2V3pCmTblkKgqlsGouKcY7lLPky6qxhuRkyDWbr8k763HvOUhtWs1HmPQmb0_T_oIozR5-m2FK8rqaXK1MeJamJIKWJnVjHvJG4MA7N1y2S3zB-3MvvuQXG8WmL7RD7ak4H9Cbh1PW7-r2ROv50_ShZThUsD3j1V2PjLqKqspLn8emwO5M-LH6fs0hyUvwEWma-2</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Liberale, Gabriel</creator><creator>Galdon, Maria Gomez</creator><creator>Moreau, Michel</creator><creator>Vankerckhove, Sophie</creator><creator>El Nakadi, Issam</creator><creator>Larsimont, Denis</creator><creator>Donckier, Vincent</creator><creator>Bourgeois, Pierre</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20160901</creationdate><title>Ex vivo detection of tumoral lymph nodes of colorectal origin with fluorescence imaging after intraoperative intravenous injection of indocyanine green</title><author>Liberale, Gabriel ; Galdon, Maria Gomez ; Moreau, Michel ; Vankerckhove, Sophie ; El Nakadi, Issam ; Larsimont, Denis ; Donckier, Vincent ; Bourgeois, Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4618-21957ae61d669e005f1ec6308044fe174592c0815fb9705dd344e68d1047222e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aged</topic><topic>colorectal neoplasms</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Colorectal Neoplasms - surgery</topic><topic>ex vivo</topic><topic>Female</topic><topic>fluorescence imaging</topic><topic>Fluorescent Dyes</topic><topic>Humans</topic><topic>Indocyanine Green</topic><topic>Lymph Node Excision</topic><topic>lymph nodes</topic><topic>Lymph Nodes - diagnostic imaging</topic><topic>Male</topic><topic>metastatic</topic><topic>Middle Aged</topic><topic>Optical Imaging</topic><topic>Peritoneal Neoplasms - diagnostic imaging</topic><topic>Peritoneal Neoplasms - secondary</topic><topic>Peritoneal Neoplasms - surgery</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liberale, Gabriel</creatorcontrib><creatorcontrib>Galdon, Maria Gomez</creatorcontrib><creatorcontrib>Moreau, Michel</creatorcontrib><creatorcontrib>Vankerckhove, Sophie</creatorcontrib><creatorcontrib>El Nakadi, Issam</creatorcontrib><creatorcontrib>Larsimont, Denis</creatorcontrib><creatorcontrib>Donckier, Vincent</creatorcontrib><creatorcontrib>Bourgeois, Pierre</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of surgical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liberale, Gabriel</au><au>Galdon, Maria Gomez</au><au>Moreau, Michel</au><au>Vankerckhove, Sophie</au><au>El Nakadi, Issam</au><au>Larsimont, Denis</au><au>Donckier, Vincent</au><au>Bourgeois, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ex vivo detection of tumoral lymph nodes of colorectal origin with fluorescence imaging after intraoperative intravenous injection of indocyanine green</atitle><jtitle>Journal of surgical oncology</jtitle><addtitle>J. Surg. Oncol</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>114</volume><issue>3</issue><spage>348</spage><epage>353</epage><pages>348-353</pages><issn>0022-4790</issn><eissn>1096-9098</eissn><abstract>Background and Objectives
The aim of this study was to investigate the potential role of indocyanine green (ICG) fluorescence imaging after intraoperative intravenous (IV) injection for the “ex vivo” detection of metastatic lymph nodes (mLNs) of colorectal cancer origin.
Methods
Fresh‐fixed LNs in cassettes and/or paraffin‐embedded LNs of patients included in a study that evaluated the role of ICG in the detection of peritoneal metastases of colorectal origin (Protocol NCT‐01995591) were further explored with a dedicated near‐infrared camera system for their fluorescence. An IV injection of ICG was delivered intraoperatively at 0.25 mg/kg. Signal to background ratios (SBRs) were calculated.
Results
LNs on operative specimens were evaluated for 12 patients (5 males, 7 females). A total of 182 LNs were analyzed. The mean LN number per patient was 15.2 (median: 15.5; range 3–22). SBRs of mLNs were significantly more fluorescent than benign LNs, 1.41 versus 1.04 arbitrary units (P < 0.0002). On univariate analysis, fluorescence was statistically correlated with LN surface area (>20 mm2) (P < 0.0004).
Conclusion
Ex vivo ICG fluorescence imaging after intraoperative IV injection represents a potential method for detecting invaded LN's of colorectal cancer origin on operative specimens. Further clinical studies are needed to better define optimal techniques. J. Surg. Oncol. 2016;114:348–353. © 2016 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>27264200</pmid><doi>10.1002/jso.24318</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-4790 |
| ispartof | Journal of surgical oncology, 2016-09, Vol.114 (3), p.348-353 |
| issn | 0022-4790 1096-9098 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1813900245 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Aged colorectal neoplasms Colorectal Neoplasms - pathology Colorectal Neoplasms - surgery ex vivo Female fluorescence imaging Fluorescent Dyes Humans Indocyanine Green Lymph Node Excision lymph nodes Lymph Nodes - diagnostic imaging Male metastatic Middle Aged Optical Imaging Peritoneal Neoplasms - diagnostic imaging Peritoneal Neoplasms - secondary Peritoneal Neoplasms - surgery Sensitivity and Specificity |
| title | Ex vivo detection of tumoral lymph nodes of colorectal origin with fluorescence imaging after intraoperative intravenous injection of indocyanine green |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T17%3A18%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ex%20vivo%20detection%20of%20tumoral%20lymph%20nodes%20of%20colorectal%20origin%20with%20fluorescence%20imaging%20after%20intraoperative%20intravenous%20injection%20of%20indocyanine%20green&rft.jtitle=Journal%20of%20surgical%20oncology&rft.au=Liberale,%20Gabriel&rft.date=2016-09-01&rft.volume=114&rft.issue=3&rft.spage=348&rft.epage=353&rft.pages=348-353&rft.issn=0022-4790&rft.eissn=1096-9098&rft_id=info:doi/10.1002/jso.24318&rft_dat=%3Cproquest_cross%3E1813900245%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1812875956&rft_id=info:pmid/27264200&rfr_iscdi=true |