Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells
Radiotherapy (RT) is one main method for the treatment of esophageal squamous cell carcinoma (ESCC), and the radioresistance is the predominant cause of patients with local recurrence. The previous results of gene microarray and subsequent verification showed that NRAGE might be involved in radiatio...
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| Veröffentlicht in: | Tumor biology 2016-07, Vol.37 (7), p.8741-8752 |
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| creator | Zhou, Huandi Zhang, Ge Xue, Xiaoying Yang, Yanling Yang, Ye Chang, Xiaojing Ge, Xiaohui Xiao, Zhiqing Guo, Han Wang, Yanqiang |
| description | Radiotherapy (RT) is one main method for the treatment of esophageal squamous cell carcinoma (ESCC), and the radioresistance is the predominant cause of patients with local recurrence. The previous results of gene microarray and subsequent verification showed that NRAGE might be involved in radiation resistance of ESCC cells. In this study, we reestablished human esophageal carcinoma radioresistant cell lines TE13R120 and ECA109R60 with gradient dose irradiation as previously reported, respectively. NRAGE expression was high in TE13R120 and ECA109R60 cells and was correlative with ionizing radiation (IR) resistance in clinic. However, the radiosensitivity of TE13R120 cells had a remarkable increase detected by colony formation assays after siRNA against NRAGE (siNRG) transfection into TE13R120 cells. Compared with TE13 cells, an increasing number of TE13R120 cells with NRAGE overexpression in S phase and a lower ratio in G2/M were observed by flow cytometry method (FCM). Intriguingly, the above changes were partially reversed in TE13R120 cells treated with siNRG. More importantly, the ectopic subcellular localization of NRAGE mediated nuclear translocation of β-catenin which may be one reason of IR resistance of esophageal carcinoma cell. These data indicate that NRAGE extremely may be a pivotal factor involved in Wnt/β-catenin signal pathway, mediating nuclear translocation of β-catenin and then facilitating the formation of radioresistance of ESCC. |
| doi_str_mv | 10.1007/s13277-015-4747-6 |
| format | Article |
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The previous results of gene microarray and subsequent verification showed that NRAGE might be involved in radiation resistance of ESCC cells. In this study, we reestablished human esophageal carcinoma radioresistant cell lines TE13R120 and ECA109R60 with gradient dose irradiation as previously reported, respectively. NRAGE expression was high in TE13R120 and ECA109R60 cells and was correlative with ionizing radiation (IR) resistance in clinic. However, the radiosensitivity of TE13R120 cells had a remarkable increase detected by colony formation assays after siRNA against NRAGE (siNRG) transfection into TE13R120 cells. Compared with TE13 cells, an increasing number of TE13R120 cells with NRAGE overexpression in S phase and a lower ratio in G2/M were observed by flow cytometry method (FCM). Intriguingly, the above changes were partially reversed in TE13R120 cells treated with siNRG. More importantly, the ectopic subcellular localization of NRAGE mediated nuclear translocation of β-catenin which may be one reason of IR resistance of esophageal carcinoma cell. These data indicate that NRAGE extremely may be a pivotal factor involved in Wnt/β-catenin signal pathway, mediating nuclear translocation of β-catenin and then facilitating the formation of radioresistance of ESCC.</description><identifier>ISSN: 1010-4283</identifier><identifier>EISSN: 1423-0380</identifier><identifier>DOI: 10.1007/s13277-015-4747-6</identifier><identifier>PMID: 26738870</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Antigens, Neoplasm - genetics ; beta Catenin - genetics ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - radiotherapy ; Cell Cycle - genetics ; Cell Line, Tumor ; Clinical outcomes ; Esophageal cancer ; Esophageal Neoplasms - genetics ; Esophageal Neoplasms - radiotherapy ; Esophageal Squamous Cell Carcinoma ; Female ; Gene expression ; Gene Expression - genetics ; Humans ; Male ; Middle Aged ; Neoplasm Proteins - genetics ; Original Article ; Radiation therapy ; Radiation Tolerance - genetics ; Radiation, Ionizing ; Radiotherapy Dosage ; RNA, Small Interfering - genetics ; Wnt Signaling Pathway - genetics ; Young Adult</subject><ispartof>Tumor biology, 2016-07, Vol.37 (7), p.8741-8752</ispartof><rights>International Society of Oncology and BioMarkers (ISOBM) 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-ea49c344b5ba45d4d753ce32c0c29f31890102ce60e3af3c838abf6071d873ca3</citedby><cites>FETCH-LOGICAL-c372t-ea49c344b5ba45d4d753ce32c0c29f31890102ce60e3af3c838abf6071d873ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13277-015-4747-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13277-015-4747-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26738870$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Huandi</creatorcontrib><creatorcontrib>Zhang, Ge</creatorcontrib><creatorcontrib>Xue, Xiaoying</creatorcontrib><creatorcontrib>Yang, Yanling</creatorcontrib><creatorcontrib>Yang, Ye</creatorcontrib><creatorcontrib>Chang, Xiaojing</creatorcontrib><creatorcontrib>Ge, Xiaohui</creatorcontrib><creatorcontrib>Xiao, Zhiqing</creatorcontrib><creatorcontrib>Guo, Han</creatorcontrib><creatorcontrib>Wang, Yanqiang</creatorcontrib><title>Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells</title><title>Tumor biology</title><addtitle>Tumor Biol</addtitle><addtitle>Tumour Biol</addtitle><description>Radiotherapy (RT) is one main method for the treatment of esophageal squamous cell carcinoma (ESCC), and the radioresistance is the predominant cause of patients with local recurrence. The previous results of gene microarray and subsequent verification showed that NRAGE might be involved in radiation resistance of ESCC cells. In this study, we reestablished human esophageal carcinoma radioresistant cell lines TE13R120 and ECA109R60 with gradient dose irradiation as previously reported, respectively. NRAGE expression was high in TE13R120 and ECA109R60 cells and was correlative with ionizing radiation (IR) resistance in clinic. However, the radiosensitivity of TE13R120 cells had a remarkable increase detected by colony formation assays after siRNA against NRAGE (siNRG) transfection into TE13R120 cells. Compared with TE13 cells, an increasing number of TE13R120 cells with NRAGE overexpression in S phase and a lower ratio in G2/M were observed by flow cytometry method (FCM). Intriguingly, the above changes were partially reversed in TE13R120 cells treated with siNRG. More importantly, the ectopic subcellular localization of NRAGE mediated nuclear translocation of β-catenin which may be one reason of IR resistance of esophageal carcinoma cell. These data indicate that NRAGE extremely may be a pivotal factor involved in Wnt/β-catenin signal pathway, mediating nuclear translocation of β-catenin and then facilitating the formation of radioresistance of ESCC.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antigens, Neoplasm - genetics</subject><subject>beta Catenin - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - radiotherapy</subject><subject>Cell Cycle - genetics</subject><subject>Cell Line, Tumor</subject><subject>Clinical outcomes</subject><subject>Esophageal cancer</subject><subject>Esophageal Neoplasms - genetics</subject><subject>Esophageal Neoplasms - radiotherapy</subject><subject>Esophageal Squamous Cell Carcinoma</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression - genetics</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasm Proteins - genetics</subject><subject>Original Article</subject><subject>Radiation therapy</subject><subject>Radiation Tolerance - genetics</subject><subject>Radiation, Ionizing</subject><subject>Radiotherapy Dosage</subject><subject>RNA, Small Interfering - genetics</subject><subject>Wnt Signaling Pathway - genetics</subject><subject>Young Adult</subject><issn>1010-4283</issn><issn>1423-0380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LxDAQhoMofv8AL1Lw4qU6yaRNehRZP0AURMFbyKZTrXSbNeku-O9NXRURPGVInnkz8zB2wOGEA6jTyFEolQMvcqmkyss1ts2lwBxQw3qqgUMuhcYtthPjKySwqspNtiVKhVor2GZP1zX1Q9u0zg6t7zPfZL1fUpfd3p9dTrK2X_puSXUqsuGFsmDr1geKbRxs72jEKfr5i30m22VuvAuZo66Le2yjsV2k_a9zlz1eTB7Or_Kbu8vr87Ob3KESQ05WVg6lnBZTK4ta1qpARygcOFE1yHWVdhCOSiC0DTqN2k6bEhSvtUJncZcdr3Lnwb8tKA5m1sZxAtuTX0TDNRdp1VJjQo_-oK9-Efo03SeFUBQAieIrygUfY6DGzEM7s-HdcDCjdrPSbpJNM2o3Zeo5_EpeTGdU_3R8e06AWAExPfXPFH59_W_qB3KSjHM</recordid><startdate>20160701</startdate><enddate>20160701</enddate><creator>Zhou, Huandi</creator><creator>Zhang, Ge</creator><creator>Xue, Xiaoying</creator><creator>Yang, Yanling</creator><creator>Yang, Ye</creator><creator>Chang, Xiaojing</creator><creator>Ge, Xiaohui</creator><creator>Xiao, Zhiqing</creator><creator>Guo, Han</creator><creator>Wang, Yanqiang</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TO</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20160701</creationdate><title>Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells</title><author>Zhou, Huandi ; 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The previous results of gene microarray and subsequent verification showed that NRAGE might be involved in radiation resistance of ESCC cells. In this study, we reestablished human esophageal carcinoma radioresistant cell lines TE13R120 and ECA109R60 with gradient dose irradiation as previously reported, respectively. NRAGE expression was high in TE13R120 and ECA109R60 cells and was correlative with ionizing radiation (IR) resistance in clinic. However, the radiosensitivity of TE13R120 cells had a remarkable increase detected by colony formation assays after siRNA against NRAGE (siNRG) transfection into TE13R120 cells. Compared with TE13 cells, an increasing number of TE13R120 cells with NRAGE overexpression in S phase and a lower ratio in G2/M were observed by flow cytometry method (FCM). Intriguingly, the above changes were partially reversed in TE13R120 cells treated with siNRG. More importantly, the ectopic subcellular localization of NRAGE mediated nuclear translocation of β-catenin which may be one reason of IR resistance of esophageal carcinoma cell. These data indicate that NRAGE extremely may be a pivotal factor involved in Wnt/β-catenin signal pathway, mediating nuclear translocation of β-catenin and then facilitating the formation of radioresistance of ESCC.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>26738870</pmid><doi>10.1007/s13277-015-4747-6</doi><tpages>12</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Antigens, Neoplasm - genetics beta Catenin - genetics Biomedical and Life Sciences Biomedicine Cancer Research Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - radiotherapy Cell Cycle - genetics Cell Line, Tumor Clinical outcomes Esophageal cancer Esophageal Neoplasms - genetics Esophageal Neoplasms - radiotherapy Esophageal Squamous Cell Carcinoma Female Gene expression Gene Expression - genetics Humans Male Middle Aged Neoplasm Proteins - genetics Original Article Radiation therapy Radiation Tolerance - genetics Radiation, Ionizing Radiotherapy Dosage RNA, Small Interfering - genetics Wnt Signaling Pathway - genetics Young Adult |
| title | Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells |
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