Accelerated follicle growth during the culture of isolated caprine preantral follicles is detrimental to follicular survival and oocyte meiotic resumption
This study investigated the effect of androstenedione (A4) alone or in association with different concentrations of bovine recombinant FSH on the IVC of isolated goat preantral follicles. Follicles were mechanically isolated from ovarian tissue and cultured for 18 days in α–minimum essential medium...
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| Veröffentlicht in: | Theriogenology 2016-10, Vol.86 (6), p.1530-1540 |
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| creator | Apolloni, Livia Brunetti Bruno, Jamily Bezerra Alves, Benner Geraldo Ferreira, Anna Clara Accioly Paes, Victor Macêdo Moreno, Jesus de los Reyes Cadenas de Aguiar, Francisco Léo Nascimento Brandão, Felipe Zandonadi Smitz, Johan Apgar, Gary de Figueiredo, José Ricardo |
| description | This study investigated the effect of androstenedione (A4) alone or in association with different concentrations of bovine recombinant FSH on the IVC of isolated goat preantral follicles. Follicles were mechanically isolated from ovarian tissue and cultured for 18 days in α–minimum essential medium supplemented or not with A4 (10 ng/mL) alone or in association with fixed (A4 + FixFSH: 100 ng/mL) or sequential (A4 + SeqFSH: Day 0, 100 ng/mL; Day 6, 500 ng/mL; Day 12, 1000 ng/mL) concentrations of FSH. After 18 days, the oocytes were recovered for IVM and fluorescence analysis. At Day 18 of culture, only A4 + SeqFSH treatment showed a lower (P 0.05). In summary, this study found that accelerated follicle growth negatively impacted the morphology of caprine preantral follicle cultured in vitro. In addition, the association of androstenedione with increasing concentration of FSH was detrimental to follicular survival and oocyte meiotic resumption. |
| doi_str_mv | 10.1016/j.theriogenology.2016.05.012 |
| format | Article |
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Follicles were mechanically isolated from ovarian tissue and cultured for 18 days in α–minimum essential medium supplemented or not with A4 (10 ng/mL) alone or in association with fixed (A4 + FixFSH: 100 ng/mL) or sequential (A4 + SeqFSH: Day 0, 100 ng/mL; Day 6, 500 ng/mL; Day 12, 1000 ng/mL) concentrations of FSH. After 18 days, the oocytes were recovered for IVM and fluorescence analysis. At Day 18 of culture, only A4 + SeqFSH treatment showed a lower (P < 0.05) rate of intact follicles, survival probability, and meiotic resumption, as well as higher (P < 0.05) percentage of degeneration and/or extrusion after antrum formation. Taken together, these results reported a positive correlation between fast-growing follicles and follicles that degenerated and/or extruded after antrum formation. When compared with control, the addition of A4 alone or in association of FSH did not increase (P > 0.05) the estradiol production or androstenedione levels on Day 6. However, on Day 18, the androstenedione levels were significantly lower in A4 + SeqFSH treatment when compared with A4 alone or to A4 + FixFSH treatments, whereas the estradiol production did not differ (P > 0.05). In summary, this study found that accelerated follicle growth negatively impacted the morphology of caprine preantral follicle cultured in vitro. In addition, the association of androstenedione with increasing concentration of FSH was detrimental to follicular survival and oocyte meiotic resumption.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2016.05.012</identifier><identifier>PMID: 27371972</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Androgens ; Androstenedione - biosynthesis ; Androstenedione - pharmacology ; Animals ; Caprine ; Cattle ; Culture Media ; Estradiol - biosynthesis ; Female ; Follicle Stimulating Hormone - administration & dosage ; Follicle Stimulating Hormone - analysis ; FSH ; Goats ; Growth rates ; In Vitro Oocyte Maturation Techniques - veterinary ; Meiosis - drug effects ; Meiosis - physiology ; Oocytes - cytology ; Ovarian Follicle - drug effects ; Ovarian Follicle - growth & development ; Ovarian Follicle - metabolism ; Recombinant Proteins ; Secondary follicles ; Tissue Culture Techniques - veterinary</subject><ispartof>Theriogenology, 2016-10, Vol.86 (6), p.1530-1540</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-10a891ec0d266a20a404a6ad1e7dc130472f54f05d5e81bc58a6522582c547363</citedby><cites>FETCH-LOGICAL-c440t-10a891ec0d266a20a404a6ad1e7dc130472f54f05d5e81bc58a6522582c547363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2016.05.012$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27371972$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Apolloni, Livia Brunetti</creatorcontrib><creatorcontrib>Bruno, Jamily Bezerra</creatorcontrib><creatorcontrib>Alves, Benner Geraldo</creatorcontrib><creatorcontrib>Ferreira, Anna Clara Accioly</creatorcontrib><creatorcontrib>Paes, Victor Macêdo</creatorcontrib><creatorcontrib>Moreno, Jesus de los Reyes Cadenas</creatorcontrib><creatorcontrib>de Aguiar, Francisco Léo Nascimento</creatorcontrib><creatorcontrib>Brandão, Felipe Zandonadi</creatorcontrib><creatorcontrib>Smitz, Johan</creatorcontrib><creatorcontrib>Apgar, Gary</creatorcontrib><creatorcontrib>de Figueiredo, José Ricardo</creatorcontrib><title>Accelerated follicle growth during the culture of isolated caprine preantral follicles is detrimental to follicular survival and oocyte meiotic resumption</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>This study investigated the effect of androstenedione (A4) alone or in association with different concentrations of bovine recombinant FSH on the IVC of isolated goat preantral follicles. Follicles were mechanically isolated from ovarian tissue and cultured for 18 days in α–minimum essential medium supplemented or not with A4 (10 ng/mL) alone or in association with fixed (A4 + FixFSH: 100 ng/mL) or sequential (A4 + SeqFSH: Day 0, 100 ng/mL; Day 6, 500 ng/mL; Day 12, 1000 ng/mL) concentrations of FSH. After 18 days, the oocytes were recovered for IVM and fluorescence analysis. At Day 18 of culture, only A4 + SeqFSH treatment showed a lower (P < 0.05) rate of intact follicles, survival probability, and meiotic resumption, as well as higher (P < 0.05) percentage of degeneration and/or extrusion after antrum formation. Taken together, these results reported a positive correlation between fast-growing follicles and follicles that degenerated and/or extruded after antrum formation. When compared with control, the addition of A4 alone or in association of FSH did not increase (P > 0.05) the estradiol production or androstenedione levels on Day 6. However, on Day 18, the androstenedione levels were significantly lower in A4 + SeqFSH treatment when compared with A4 alone or to A4 + FixFSH treatments, whereas the estradiol production did not differ (P > 0.05). In summary, this study found that accelerated follicle growth negatively impacted the morphology of caprine preantral follicle cultured in vitro. In addition, the association of androstenedione with increasing concentration of FSH was detrimental to follicular survival and oocyte meiotic resumption.</description><subject>Androgens</subject><subject>Androstenedione - biosynthesis</subject><subject>Androstenedione - pharmacology</subject><subject>Animals</subject><subject>Caprine</subject><subject>Cattle</subject><subject>Culture Media</subject><subject>Estradiol - biosynthesis</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - administration & dosage</subject><subject>Follicle Stimulating Hormone - analysis</subject><subject>FSH</subject><subject>Goats</subject><subject>Growth rates</subject><subject>In Vitro Oocyte Maturation Techniques - veterinary</subject><subject>Meiosis - drug effects</subject><subject>Meiosis - physiology</subject><subject>Oocytes - cytology</subject><subject>Ovarian Follicle - drug effects</subject><subject>Ovarian Follicle - growth & development</subject><subject>Ovarian Follicle - metabolism</subject><subject>Recombinant Proteins</subject><subject>Secondary follicles</subject><subject>Tissue Culture Techniques - veterinary</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2LFDEQhoMo7rj6FyQHD166raST_gAvy-KqsOBFwVvIJtWzGdKdMR8j81f8tWadccGbp4J6n6qXqpeQNwxaBqx_t2vzPUYXtrgGH7bHltduC7IFxp-QDRuHqel4x56SDcDUNf3Evl-QFyntAKDre_acXPChG9g08A35dWUMeow6o6Vz8N4Zj3Qbw898T22Jbt3S6kdN8blEpGGmLgX_Bzd6X3Wk-4h6zVH7xwWpQtRijm7BNVchh7NWvI40lXhwh9rWq6UhmGNGuqAL2RkaMZVln11YX5Jns_YJX53rJfl28-Hr9afm9svHz9dXt40RAnLDQI8TQwOW973moAUI3WvLcLCGdSAGPksxg7QSR3Zn5Kh7ybkcuZFi6Prukrw97d3H8KNgympxqT7F6xVDSYqNjAvBhISKvj-hJoaUIs6qfmDR8agYqId01E79m456SEeBVDWdOv767FTuFrSPw3_jqMDNCcB678FhVMk4XA1aF9FkZYP7P6ffIVSuHA</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Apolloni, Livia Brunetti</creator><creator>Bruno, Jamily Bezerra</creator><creator>Alves, Benner Geraldo</creator><creator>Ferreira, Anna Clara Accioly</creator><creator>Paes, Victor Macêdo</creator><creator>Moreno, Jesus de los Reyes Cadenas</creator><creator>de Aguiar, Francisco Léo Nascimento</creator><creator>Brandão, Felipe Zandonadi</creator><creator>Smitz, Johan</creator><creator>Apgar, Gary</creator><creator>de Figueiredo, José Ricardo</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20161001</creationdate><title>Accelerated follicle growth during the culture of isolated caprine preantral follicles is detrimental to follicular survival and oocyte meiotic resumption</title><author>Apolloni, Livia Brunetti ; Bruno, Jamily Bezerra ; Alves, Benner Geraldo ; Ferreira, Anna Clara Accioly ; Paes, Victor Macêdo ; Moreno, Jesus de los Reyes Cadenas ; de Aguiar, Francisco Léo Nascimento ; Brandão, Felipe Zandonadi ; Smitz, Johan ; Apgar, Gary ; de Figueiredo, José Ricardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-10a891ec0d266a20a404a6ad1e7dc130472f54f05d5e81bc58a6522582c547363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Androgens</topic><topic>Androstenedione - biosynthesis</topic><topic>Androstenedione - pharmacology</topic><topic>Animals</topic><topic>Caprine</topic><topic>Cattle</topic><topic>Culture Media</topic><topic>Estradiol - biosynthesis</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - administration & dosage</topic><topic>Follicle Stimulating Hormone - analysis</topic><topic>FSH</topic><topic>Goats</topic><topic>Growth rates</topic><topic>In Vitro Oocyte Maturation Techniques - veterinary</topic><topic>Meiosis - drug effects</topic><topic>Meiosis - physiology</topic><topic>Oocytes - cytology</topic><topic>Ovarian Follicle - drug effects</topic><topic>Ovarian Follicle - growth & development</topic><topic>Ovarian Follicle - metabolism</topic><topic>Recombinant Proteins</topic><topic>Secondary follicles</topic><topic>Tissue Culture Techniques - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Apolloni, Livia Brunetti</creatorcontrib><creatorcontrib>Bruno, Jamily Bezerra</creatorcontrib><creatorcontrib>Alves, Benner Geraldo</creatorcontrib><creatorcontrib>Ferreira, Anna Clara Accioly</creatorcontrib><creatorcontrib>Paes, Victor Macêdo</creatorcontrib><creatorcontrib>Moreno, Jesus de los Reyes Cadenas</creatorcontrib><creatorcontrib>de Aguiar, Francisco Léo Nascimento</creatorcontrib><creatorcontrib>Brandão, Felipe Zandonadi</creatorcontrib><creatorcontrib>Smitz, Johan</creatorcontrib><creatorcontrib>Apgar, Gary</creatorcontrib><creatorcontrib>de Figueiredo, José Ricardo</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Apolloni, Livia Brunetti</au><au>Bruno, Jamily Bezerra</au><au>Alves, Benner Geraldo</au><au>Ferreira, Anna Clara Accioly</au><au>Paes, Victor Macêdo</au><au>Moreno, Jesus de los Reyes Cadenas</au><au>de Aguiar, Francisco Léo Nascimento</au><au>Brandão, Felipe Zandonadi</au><au>Smitz, Johan</au><au>Apgar, Gary</au><au>de Figueiredo, José Ricardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accelerated follicle growth during the culture of isolated caprine preantral follicles is detrimental to follicular survival and oocyte meiotic resumption</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>86</volume><issue>6</issue><spage>1530</spage><epage>1540</epage><pages>1530-1540</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>This study investigated the effect of androstenedione (A4) alone or in association with different concentrations of bovine recombinant FSH on the IVC of isolated goat preantral follicles. Follicles were mechanically isolated from ovarian tissue and cultured for 18 days in α–minimum essential medium supplemented or not with A4 (10 ng/mL) alone or in association with fixed (A4 + FixFSH: 100 ng/mL) or sequential (A4 + SeqFSH: Day 0, 100 ng/mL; Day 6, 500 ng/mL; Day 12, 1000 ng/mL) concentrations of FSH. After 18 days, the oocytes were recovered for IVM and fluorescence analysis. At Day 18 of culture, only A4 + SeqFSH treatment showed a lower (P < 0.05) rate of intact follicles, survival probability, and meiotic resumption, as well as higher (P < 0.05) percentage of degeneration and/or extrusion after antrum formation. Taken together, these results reported a positive correlation between fast-growing follicles and follicles that degenerated and/or extruded after antrum formation. When compared with control, the addition of A4 alone or in association of FSH did not increase (P > 0.05) the estradiol production or androstenedione levels on Day 6. However, on Day 18, the androstenedione levels were significantly lower in A4 + SeqFSH treatment when compared with A4 alone or to A4 + FixFSH treatments, whereas the estradiol production did not differ (P > 0.05). In summary, this study found that accelerated follicle growth negatively impacted the morphology of caprine preantral follicle cultured in vitro. In addition, the association of androstenedione with increasing concentration of FSH was detrimental to follicular survival and oocyte meiotic resumption.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27371972</pmid><doi>10.1016/j.theriogenology.2016.05.012</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Androgens Androstenedione - biosynthesis Androstenedione - pharmacology Animals Caprine Cattle Culture Media Estradiol - biosynthesis Female Follicle Stimulating Hormone - administration & dosage Follicle Stimulating Hormone - analysis FSH Goats Growth rates In Vitro Oocyte Maturation Techniques - veterinary Meiosis - drug effects Meiosis - physiology Oocytes - cytology Ovarian Follicle - drug effects Ovarian Follicle - growth & development Ovarian Follicle - metabolism Recombinant Proteins Secondary follicles Tissue Culture Techniques - veterinary |
| title | Accelerated follicle growth during the culture of isolated caprine preantral follicles is detrimental to follicular survival and oocyte meiotic resumption |
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