Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake
Mitogen-activated protein kinase kinase kinase 5 (MAP3K5) is essential for apoptosis, proliferation, differentiation, and immune responses, and is a candidate marker for residual feed intake (RFI) in pig. We cloned the full-length cDNA sequence of porcine MAP3K5 by rapid-amplification of cDNA ends....
Gespeichert in:
| Veröffentlicht in: | Genetics and molecular research 2016-08, Vol.15 (3) |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 3 |
| container_start_page | |
| container_title | Genetics and molecular research |
| container_volume | 15 |
| creator | Pu, L Zhang, L C Zhang, J S Song, X Wang, L G Liang, J Zhang, Y B Liu, X Yan, H Zhang, T Yue, J W Li, N Wu, Q Q Wang, L X |
| description | Mitogen-activated protein kinase kinase kinase 5 (MAP3K5) is essential for apoptosis, proliferation, differentiation, and immune responses, and is a candidate marker for residual feed intake (RFI) in pig. We cloned the full-length cDNA sequence of porcine MAP3K5 by rapid-amplification of cDNA ends. The 5451-bp gene contains a 5'-untranslated region (UTR) (718 bp), a coding region (3738 bp), and a 3'-UTR (995 bp), and encodes a peptide of 1245 amino acids, which shares 97, 99, 97, 93, 91, and 84% sequence identity with cattle, sheep, human, mouse, chicken, and zebrafish MAP3K5, respectively. The deduced MAP3K5 protein sequence contains two conserved domains: a DUF4071 domain and a protein kinase domain. Phylogenetic analysis showed that porcine MAP3K5 forms a separate branch to vicugna and camel MAP3K5. Tissue expression analysis using real-time quantitative polymerase chain reaction (qRT-PCR) revealed that MAP3K5 was expressed in the heart, liver, spleen, lung, kidney, muscle, fat, pancrea, ileum, and stomach tissues. Copy number variation was detected for porcine MAP3K5 and validated by qRT-PCR. Furthermore, a significant increase in average copy number was detected in the low RFI group when compared to the high RFI group in a Duroc pig population. These results provide useful information regarding the influence of MAP3K5 on RFI in pigs. |
| doi_str_mv | 10.4238/gmr.15037998 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1812224292</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1812224292</sourcerecordid><originalsourceid>FETCH-LOGICAL-c329t-932edfba294d7f07aa88b26cd15937c01f4979ce44007e39b14e130ecb59fbdf3</originalsourceid><addsrcrecordid>eNpNkUtP3DAQxy3Uil0ot56Rjz3sUj-STcxthXhUUJVDOUcTZwIuiR08CXT5JP24NY-teprXb_7SzJ-xz1IcZUqXX2_7eCRzoQtjyh02l6titcxXpfjwXz5je0S_hFB5VopdNlNFrnKj9Zz9uQ7ROo_8-_paX-YcPHQbcnTM-9ChnTqI3HbBO3-74PYOItgRo3uG0QW_4KMjmpDj7yEiUWrxAcYEpBH4htswbLif-hojf4ToXreIA1GwqcCGP7nxjqdd10zQ8RZTy_kR7vET-9hCR3jwHvfZzdnpz5OL5dWP828n66ul1cqMS6MVNm0NymRN0YoCoCxrtbKNTPcVVsg2M4WxmGVCFKhNLTOUWqCtc9PWTav32Zc33SGGhwlprHpHFrsOPIaJKllKpVSmjEro4g21MRBFbKshuh7ippKievGiSl5UWy8SfviuPNU9Nv_g7fP1X9NtiIk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1812224292</pqid></control><display><type>article</type><title>Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Pu, L ; Zhang, L C ; Zhang, J S ; Song, X ; Wang, L G ; Liang, J ; Zhang, Y B ; Liu, X ; Yan, H ; Zhang, T ; Yue, J W ; Li, N ; Wu, Q Q ; Wang, L X</creator><creatorcontrib>Pu, L ; Zhang, L C ; Zhang, J S ; Song, X ; Wang, L G ; Liang, J ; Zhang, Y B ; Liu, X ; Yan, H ; Zhang, T ; Yue, J W ; Li, N ; Wu, Q Q ; Wang, L X</creatorcontrib><description>Mitogen-activated protein kinase kinase kinase 5 (MAP3K5) is essential for apoptosis, proliferation, differentiation, and immune responses, and is a candidate marker for residual feed intake (RFI) in pig. We cloned the full-length cDNA sequence of porcine MAP3K5 by rapid-amplification of cDNA ends. The 5451-bp gene contains a 5'-untranslated region (UTR) (718 bp), a coding region (3738 bp), and a 3'-UTR (995 bp), and encodes a peptide of 1245 amino acids, which shares 97, 99, 97, 93, 91, and 84% sequence identity with cattle, sheep, human, mouse, chicken, and zebrafish MAP3K5, respectively. The deduced MAP3K5 protein sequence contains two conserved domains: a DUF4071 domain and a protein kinase domain. Phylogenetic analysis showed that porcine MAP3K5 forms a separate branch to vicugna and camel MAP3K5. Tissue expression analysis using real-time quantitative polymerase chain reaction (qRT-PCR) revealed that MAP3K5 was expressed in the heart, liver, spleen, lung, kidney, muscle, fat, pancrea, ileum, and stomach tissues. Copy number variation was detected for porcine MAP3K5 and validated by qRT-PCR. Furthermore, a significant increase in average copy number was detected in the low RFI group when compared to the high RFI group in a Duroc pig population. These results provide useful information regarding the influence of MAP3K5 on RFI in pigs.</description><identifier>ISSN: 1676-5680</identifier><identifier>EISSN: 1676-5680</identifier><identifier>DOI: 10.4238/gmr.15037998</identifier><identifier>PMID: 27525933</identifier><language>eng</language><publisher>Brazil</publisher><subject>Amino Acid Sequence ; Animals ; Catalytic Domain ; Cloning, Molecular ; DNA Copy Number Variations ; Gene Dosage ; Gene Expression ; Hydrophobic and Hydrophilic Interactions ; MAP Kinase Kinase Kinase 5 - chemistry ; MAP Kinase Kinase Kinase 5 - genetics ; MAP Kinase Kinase Kinase 5 - metabolism ; Models, Molecular ; Organ Specificity ; Phylogeny ; Protein Conformation, alpha-Helical ; Sus scrofa - genetics ; Sus scrofa - metabolism</subject><ispartof>Genetics and molecular research, 2016-08, Vol.15 (3)</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c329t-932edfba294d7f07aa88b26cd15937c01f4979ce44007e39b14e130ecb59fbdf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27525933$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pu, L</creatorcontrib><creatorcontrib>Zhang, L C</creatorcontrib><creatorcontrib>Zhang, J S</creatorcontrib><creatorcontrib>Song, X</creatorcontrib><creatorcontrib>Wang, L G</creatorcontrib><creatorcontrib>Liang, J</creatorcontrib><creatorcontrib>Zhang, Y B</creatorcontrib><creatorcontrib>Liu, X</creatorcontrib><creatorcontrib>Yan, H</creatorcontrib><creatorcontrib>Zhang, T</creatorcontrib><creatorcontrib>Yue, J W</creatorcontrib><creatorcontrib>Li, N</creatorcontrib><creatorcontrib>Wu, Q Q</creatorcontrib><creatorcontrib>Wang, L X</creatorcontrib><title>Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake</title><title>Genetics and molecular research</title><addtitle>Genet Mol Res</addtitle><description>Mitogen-activated protein kinase kinase kinase 5 (MAP3K5) is essential for apoptosis, proliferation, differentiation, and immune responses, and is a candidate marker for residual feed intake (RFI) in pig. We cloned the full-length cDNA sequence of porcine MAP3K5 by rapid-amplification of cDNA ends. The 5451-bp gene contains a 5'-untranslated region (UTR) (718 bp), a coding region (3738 bp), and a 3'-UTR (995 bp), and encodes a peptide of 1245 amino acids, which shares 97, 99, 97, 93, 91, and 84% sequence identity with cattle, sheep, human, mouse, chicken, and zebrafish MAP3K5, respectively. The deduced MAP3K5 protein sequence contains two conserved domains: a DUF4071 domain and a protein kinase domain. Phylogenetic analysis showed that porcine MAP3K5 forms a separate branch to vicugna and camel MAP3K5. Tissue expression analysis using real-time quantitative polymerase chain reaction (qRT-PCR) revealed that MAP3K5 was expressed in the heart, liver, spleen, lung, kidney, muscle, fat, pancrea, ileum, and stomach tissues. Copy number variation was detected for porcine MAP3K5 and validated by qRT-PCR. Furthermore, a significant increase in average copy number was detected in the low RFI group when compared to the high RFI group in a Duroc pig population. These results provide useful information regarding the influence of MAP3K5 on RFI in pigs.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Catalytic Domain</subject><subject>Cloning, Molecular</subject><subject>DNA Copy Number Variations</subject><subject>Gene Dosage</subject><subject>Gene Expression</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>MAP Kinase Kinase Kinase 5 - chemistry</subject><subject>MAP Kinase Kinase Kinase 5 - genetics</subject><subject>MAP Kinase Kinase Kinase 5 - metabolism</subject><subject>Models, Molecular</subject><subject>Organ Specificity</subject><subject>Phylogeny</subject><subject>Protein Conformation, alpha-Helical</subject><subject>Sus scrofa - genetics</subject><subject>Sus scrofa - metabolism</subject><issn>1676-5680</issn><issn>1676-5680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkUtP3DAQxy3Uil0ot56Rjz3sUj-STcxthXhUUJVDOUcTZwIuiR08CXT5JP24NY-teprXb_7SzJ-xz1IcZUqXX2_7eCRzoQtjyh02l6titcxXpfjwXz5je0S_hFB5VopdNlNFrnKj9Zz9uQ7ROo_8-_paX-YcPHQbcnTM-9ChnTqI3HbBO3-74PYOItgRo3uG0QW_4KMjmpDj7yEiUWrxAcYEpBH4htswbLif-hojf4ToXreIA1GwqcCGP7nxjqdd10zQ8RZTy_kR7vET-9hCR3jwHvfZzdnpz5OL5dWP828n66ul1cqMS6MVNm0NymRN0YoCoCxrtbKNTPcVVsg2M4WxmGVCFKhNLTOUWqCtc9PWTav32Zc33SGGhwlprHpHFrsOPIaJKllKpVSmjEro4g21MRBFbKshuh7ippKievGiSl5UWy8SfviuPNU9Nv_g7fP1X9NtiIk</recordid><startdate>20160812</startdate><enddate>20160812</enddate><creator>Pu, L</creator><creator>Zhang, L C</creator><creator>Zhang, J S</creator><creator>Song, X</creator><creator>Wang, L G</creator><creator>Liang, J</creator><creator>Zhang, Y B</creator><creator>Liu, X</creator><creator>Yan, H</creator><creator>Zhang, T</creator><creator>Yue, J W</creator><creator>Li, N</creator><creator>Wu, Q Q</creator><creator>Wang, L X</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160812</creationdate><title>Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake</title><author>Pu, L ; Zhang, L C ; Zhang, J S ; Song, X ; Wang, L G ; Liang, J ; Zhang, Y B ; Liu, X ; Yan, H ; Zhang, T ; Yue, J W ; Li, N ; Wu, Q Q ; Wang, L X</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-932edfba294d7f07aa88b26cd15937c01f4979ce44007e39b14e130ecb59fbdf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Catalytic Domain</topic><topic>Cloning, Molecular</topic><topic>DNA Copy Number Variations</topic><topic>Gene Dosage</topic><topic>Gene Expression</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>MAP Kinase Kinase Kinase 5 - chemistry</topic><topic>MAP Kinase Kinase Kinase 5 - genetics</topic><topic>MAP Kinase Kinase Kinase 5 - metabolism</topic><topic>Models, Molecular</topic><topic>Organ Specificity</topic><topic>Phylogeny</topic><topic>Protein Conformation, alpha-Helical</topic><topic>Sus scrofa - genetics</topic><topic>Sus scrofa - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pu, L</creatorcontrib><creatorcontrib>Zhang, L C</creatorcontrib><creatorcontrib>Zhang, J S</creatorcontrib><creatorcontrib>Song, X</creatorcontrib><creatorcontrib>Wang, L G</creatorcontrib><creatorcontrib>Liang, J</creatorcontrib><creatorcontrib>Zhang, Y B</creatorcontrib><creatorcontrib>Liu, X</creatorcontrib><creatorcontrib>Yan, H</creatorcontrib><creatorcontrib>Zhang, T</creatorcontrib><creatorcontrib>Yue, J W</creatorcontrib><creatorcontrib>Li, N</creatorcontrib><creatorcontrib>Wu, Q Q</creatorcontrib><creatorcontrib>Wang, L X</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pu, L</au><au>Zhang, L C</au><au>Zhang, J S</au><au>Song, X</au><au>Wang, L G</au><au>Liang, J</au><au>Zhang, Y B</au><au>Liu, X</au><au>Yan, H</au><au>Zhang, T</au><au>Yue, J W</au><au>Li, N</au><au>Wu, Q Q</au><au>Wang, L X</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2016-08-12</date><risdate>2016</risdate><volume>15</volume><issue>3</issue><issn>1676-5680</issn><eissn>1676-5680</eissn><abstract>Mitogen-activated protein kinase kinase kinase 5 (MAP3K5) is essential for apoptosis, proliferation, differentiation, and immune responses, and is a candidate marker for residual feed intake (RFI) in pig. We cloned the full-length cDNA sequence of porcine MAP3K5 by rapid-amplification of cDNA ends. The 5451-bp gene contains a 5'-untranslated region (UTR) (718 bp), a coding region (3738 bp), and a 3'-UTR (995 bp), and encodes a peptide of 1245 amino acids, which shares 97, 99, 97, 93, 91, and 84% sequence identity with cattle, sheep, human, mouse, chicken, and zebrafish MAP3K5, respectively. The deduced MAP3K5 protein sequence contains two conserved domains: a DUF4071 domain and a protein kinase domain. Phylogenetic analysis showed that porcine MAP3K5 forms a separate branch to vicugna and camel MAP3K5. Tissue expression analysis using real-time quantitative polymerase chain reaction (qRT-PCR) revealed that MAP3K5 was expressed in the heart, liver, spleen, lung, kidney, muscle, fat, pancrea, ileum, and stomach tissues. Copy number variation was detected for porcine MAP3K5 and validated by qRT-PCR. Furthermore, a significant increase in average copy number was detected in the low RFI group when compared to the high RFI group in a Duroc pig population. These results provide useful information regarding the influence of MAP3K5 on RFI in pigs.</abstract><cop>Brazil</cop><pmid>27525933</pmid><doi>10.4238/gmr.15037998</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1676-5680 |
| ispartof | Genetics and molecular research, 2016-08, Vol.15 (3) |
| issn | 1676-5680 1676-5680 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1812224292 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence Animals Catalytic Domain Cloning, Molecular DNA Copy Number Variations Gene Dosage Gene Expression Hydrophobic and Hydrophilic Interactions MAP Kinase Kinase Kinase 5 - chemistry MAP Kinase Kinase Kinase 5 - genetics MAP Kinase Kinase Kinase 5 - metabolism Models, Molecular Organ Specificity Phylogeny Protein Conformation, alpha-Helical Sus scrofa - genetics Sus scrofa - metabolism |
| title | Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T12%3A53%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Porcine%20MAP3K5%20analysis:%20molecular%20cloning,%20characterization,%20tissue%20expression%20pattern,%20and%20copy%20number%20variations%20associated%20with%20residual%20feed%20intake&rft.jtitle=Genetics%20and%20molecular%20research&rft.au=Pu,%20L&rft.date=2016-08-12&rft.volume=15&rft.issue=3&rft.issn=1676-5680&rft.eissn=1676-5680&rft_id=info:doi/10.4238/gmr.15037998&rft_dat=%3Cproquest_cross%3E1812224292%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1812224292&rft_id=info:pmid/27525933&rfr_iscdi=true |