A Model of Cadmium Uptake and Transport in Caco-2 Cells
We created a physiologically substantiated kinetic model of cadmium transport and toxicity in intestinal cell model (Caco-2 cells). Transcriptome profiling of Caco-2 cells revealed high content of transporter DMT1 and ZIP14 and intensive expression of some calcium channels of the CACN family. The ma...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2016-05, Vol.161 (1), p.187-192 |
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| container_title | Bulletin of experimental biology and medicine |
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| creator | Gerasimenko, T. N. Senyavina, N. V. Anisimov, N. U. Tonevitskaya, S. A. |
| description | We created a physiologically substantiated kinetic model of cadmium transport and toxicity in intestinal cell model (Caco-2 cells). Transcriptome profiling of Caco-2 cells revealed high content of transporter DMT1 and ZIP14 and intensive expression of some calcium channels of the CACN family. The mathematical model describing three types of transporters, as well as intracellular cadmium binding with metallothionein and excretion through the basolateral membrane allowed us to construct cadmium uptake and transport curves that approximated the previously obtained experimental data. Using the proposed model, we determined toxic intracellular cadmium concentration leading to cell death and impairing the integrity of cell monolayer and described cadmium transport in this case. |
| doi_str_mv | 10.1007/s10517-016-3373-7 |
| format | Article |
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N. ; Senyavina, N. V. ; Anisimov, N. U. ; Tonevitskaya, S. A.</creator><creatorcontrib>Gerasimenko, T. N. ; Senyavina, N. V. ; Anisimov, N. U. ; Tonevitskaya, S. A.</creatorcontrib><description>We created a physiologically substantiated kinetic model of cadmium transport and toxicity in intestinal cell model (Caco-2 cells). Transcriptome profiling of Caco-2 cells revealed high content of transporter DMT1 and ZIP14 and intensive expression of some calcium channels of the CACN family. The mathematical model describing three types of transporters, as well as intracellular cadmium binding with metallothionein and excretion through the basolateral membrane allowed us to construct cadmium uptake and transport curves that approximated the previously obtained experimental data. 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Using the proposed model, we determined toxic intracellular cadmium concentration leading to cell death and impairing the integrity of cell monolayer and described cadmium transport in this case.</description><subject>Analysis</subject><subject>Biological Transport</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Caco-2 Cells</subject><subject>Cadmium</subject><subject>Cadmium - metabolism</subject><subject>Calcium channels</subject><subject>Calcium Channels - metabolism</subject><subject>Cation Transport Proteins - metabolism</subject><subject>Cell Biology</subject><subject>Cell death</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Kinetics</subject><subject>Laboratory Medicine</subject><subject>Metallothionein</subject><subject>Models, Biological</subject><subject>Pathology</subject><subject>Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kUtLxDAUhYMoOo7-ADdSEMRNNY-mSZbD4AsUN-M6pEmq1TYZk3bhvzdlRh1FySKP-53DvTkAHCF4jiBkFxFBilgOUZkTwkjOtsAE0XTgGKNtMIEJygvO-R7Yj_FlvMIS7YI9zDAVhWATwGbZvTe2zXydzZXpmqHLHpe9erWZciZbBOXi0oc-a1yqa5_jbG7bNh6AnVq10R6u9yl4vLpczG_yu4fr2_nsLtcUkj6vLWXccFjB0mCsGTVM16giFS9VVVlMlaaUk7KiqNBUY1aU1AhRWF7DwmBNpuBs5bsM_m2wsZddE3XqQDnrhygRR0hAwgVP6Mkv9MUPwaXuElVCLrBA4pt6Uq2Vjat9H5QeTeWsEDw1Q9IPTsH5H1RaxnaN9s7WTXr_ITjdEDxb1fbP0bdD33gXf4JoBergYwy2lsvQdCq8SwTlmKpcpSpTqnJMVY6a4_VkQ9VZ86X4jDEBeAXEVHJPNmyM_q_rB4bzpvg</recordid><startdate>20160501</startdate><enddate>20160501</enddate><creator>Gerasimenko, T. 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N. ; Senyavina, N. V. ; Anisimov, N. U. ; Tonevitskaya, S. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-fe578d80b06d22c75d7cf1b3b86abbe25ac55836b514c5c27465d994e8f04d2c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis</topic><topic>Biological Transport</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Caco-2 Cells</topic><topic>Cadmium</topic><topic>Cadmium - metabolism</topic><topic>Calcium channels</topic><topic>Calcium Channels - metabolism</topic><topic>Cation Transport Proteins - metabolism</topic><topic>Cell Biology</topic><topic>Cell death</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Kinetics</topic><topic>Laboratory Medicine</topic><topic>Metallothionein</topic><topic>Models, Biological</topic><topic>Pathology</topic><topic>Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gerasimenko, T. 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| subjects | Analysis Biological Transport Biomedical and Life Sciences Biomedicine Caco-2 Cells Cadmium Cadmium - metabolism Calcium channels Calcium Channels - metabolism Cation Transport Proteins - metabolism Cell Biology Cell death Humans Internal Medicine Kinetics Laboratory Medicine Metallothionein Models, Biological Pathology Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine) |
| title | A Model of Cadmium Uptake and Transport in Caco-2 Cells |
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