Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells
Objectives The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β 1 (TGF-β 1 ) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED). Mate...
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Veröffentlicht in: | Clinical oral investigations 2016-07, Vol.20 (6), p.1181-1191 |
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creator | Farea, Manal Husein, Adam Halim, Ahmad Sukari Berahim, Zurairah Nurul, Asma Abdullah Mokhtar, Khairani Idah Mokhtar, Kasmawati |
description | Objectives
The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β
1
(TGF-β
1
) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED).
Materials and methods
SHED were co-cultured with ERM with/without TGF-β
1
. Then, SHED proliferation, morphological appearance, alkaline phosphatase (
ALP
) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated.
Results
TGF-β
1
enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β
1
. This activity was demonstrated by high
ALP
activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e.
ALP
, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein).
Conclusions
ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β
1
.
Clinical relevance
The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering. |
doi_str_mv | 10.1007/s00784-015-1601-6 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1811903225</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1798994611</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-28dc46e452bf98597bac71b0fc43b4e6a65b6b59f2bf803b5ed7fb960ff36ffd3</originalsourceid><addsrcrecordid>eNqFkc2OFSEQhYnROOPoA7gxJG7coPw13SzNjX_JGDe6JtBdzGXshivQGfUpfGS5t0djTIwbIKmvTp3iIPSY0eeM0v5FaccgCWUdYYoyou6gcyaFIqLv2d3TmxOlB3aGHpRyTSmTqhf30RlXQnOh1Tn6sYMFYk1utqWGEc8hgr0C7FNebA0p4hBx3QMecyqFVDt_xg7qDUDEpcKCR5jngpPH-3WxEcNXn-ZgK0x4gjFMa1oLrgB1j22cMBxCE2vAjDOUemp8b9vsAt83qYfonrdzgUe39wX69PrVx91bcvnhzbvdy0syStpVwodplApkx53XQ6d7Z8eeOepHKZwEZVXnlOu0b_WBCtfB1HunFfVeKO8ncYGebbqHnL6szYtZQjk6sBGaZ8MGxjQVnHf_R3s9aC0VYw19-hd6ndYc2yInilM2qCPFNur0qRm8OeSw2PzNMGqOyZotWdOSNcdkjWo9T26VV7fA9LvjV5QN4BtQWileQf5j9D9VfwLJq7DA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1798201861</pqid></control><display><type>article</type><title>Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Farea, Manal ; Husein, Adam ; Halim, Ahmad Sukari ; Berahim, Zurairah ; Nurul, Asma Abdullah ; Mokhtar, Khairani Idah ; Mokhtar, Kasmawati</creator><creatorcontrib>Farea, Manal ; Husein, Adam ; Halim, Ahmad Sukari ; Berahim, Zurairah ; Nurul, Asma Abdullah ; Mokhtar, Khairani Idah ; Mokhtar, Kasmawati</creatorcontrib><description>Objectives
The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β
1
(TGF-β
1
) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED).
Materials and methods
SHED were co-cultured with ERM with/without TGF-β
1
. Then, SHED proliferation, morphological appearance, alkaline phosphatase (
ALP
) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated.
Results
TGF-β
1
enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β
1
. This activity was demonstrated by high
ALP
activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e.
ALP
, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein).
Conclusions
ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β
1
.
Clinical relevance
The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-015-1601-6</identifier><identifier>PMID: 26392396</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Cell Differentiation - physiology ; Cell Proliferation - physiology ; Coculture Techniques ; Dental Cementum - cytology ; Dental Enamel Proteins - metabolism ; Dentistry ; Epithelial Cells - cytology ; Gene Expression ; Humans ; Medicine ; Original Article ; Osteogenesis - physiology ; Phenotype ; Stem Cells - cytology ; Tooth, Deciduous - cytology ; Transforming Growth Factor beta1 - pharmacology</subject><ispartof>Clinical oral investigations, 2016-07, Vol.20 (6), p.1181-1191</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-28dc46e452bf98597bac71b0fc43b4e6a65b6b59f2bf803b5ed7fb960ff36ffd3</citedby><cites>FETCH-LOGICAL-c405t-28dc46e452bf98597bac71b0fc43b4e6a65b6b59f2bf803b5ed7fb960ff36ffd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-015-1601-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-015-1601-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26392396$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Farea, Manal</creatorcontrib><creatorcontrib>Husein, Adam</creatorcontrib><creatorcontrib>Halim, Ahmad Sukari</creatorcontrib><creatorcontrib>Berahim, Zurairah</creatorcontrib><creatorcontrib>Nurul, Asma Abdullah</creatorcontrib><creatorcontrib>Mokhtar, Khairani Idah</creatorcontrib><creatorcontrib>Mokhtar, Kasmawati</creatorcontrib><title>Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β
1
(TGF-β
1
) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED).
Materials and methods
SHED were co-cultured with ERM with/without TGF-β
1
. Then, SHED proliferation, morphological appearance, alkaline phosphatase (
ALP
) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated.
Results
TGF-β
1
enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β
1
. This activity was demonstrated by high
ALP
activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e.
ALP
, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein).
Conclusions
ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β
1
.
Clinical relevance
The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.</description><subject>Cell Differentiation - physiology</subject><subject>Cell Proliferation - physiology</subject><subject>Coculture Techniques</subject><subject>Dental Cementum - cytology</subject><subject>Dental Enamel Proteins - metabolism</subject><subject>Dentistry</subject><subject>Epithelial Cells - cytology</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Medicine</subject><subject>Original Article</subject><subject>Osteogenesis - physiology</subject><subject>Phenotype</subject><subject>Stem Cells - cytology</subject><subject>Tooth, Deciduous - cytology</subject><subject>Transforming Growth Factor beta1 - pharmacology</subject><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc2OFSEQhYnROOPoA7gxJG7coPw13SzNjX_JGDe6JtBdzGXshivQGfUpfGS5t0djTIwbIKmvTp3iIPSY0eeM0v5FaccgCWUdYYoyou6gcyaFIqLv2d3TmxOlB3aGHpRyTSmTqhf30RlXQnOh1Tn6sYMFYk1utqWGEc8hgr0C7FNebA0p4hBx3QMecyqFVDt_xg7qDUDEpcKCR5jngpPH-3WxEcNXn-ZgK0x4gjFMa1oLrgB1j22cMBxCE2vAjDOUemp8b9vsAt83qYfonrdzgUe39wX69PrVx91bcvnhzbvdy0syStpVwodplApkx53XQ6d7Z8eeOepHKZwEZVXnlOu0b_WBCtfB1HunFfVeKO8ncYGebbqHnL6szYtZQjk6sBGaZ8MGxjQVnHf_R3s9aC0VYw19-hd6ndYc2yInilM2qCPFNur0qRm8OeSw2PzNMGqOyZotWdOSNcdkjWo9T26VV7fA9LvjV5QN4BtQWileQf5j9D9VfwLJq7DA</recordid><startdate>20160701</startdate><enddate>20160701</enddate><creator>Farea, Manal</creator><creator>Husein, Adam</creator><creator>Halim, Ahmad Sukari</creator><creator>Berahim, Zurairah</creator><creator>Nurul, Asma Abdullah</creator><creator>Mokhtar, Khairani Idah</creator><creator>Mokhtar, Kasmawati</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>20160701</creationdate><title>Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells</title><author>Farea, Manal ; Husein, Adam ; Halim, Ahmad Sukari ; Berahim, Zurairah ; Nurul, Asma Abdullah ; Mokhtar, Khairani Idah ; Mokhtar, Kasmawati</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-28dc46e452bf98597bac71b0fc43b4e6a65b6b59f2bf803b5ed7fb960ff36ffd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Cell Differentiation - physiology</topic><topic>Cell Proliferation - physiology</topic><topic>Coculture Techniques</topic><topic>Dental Cementum - cytology</topic><topic>Dental Enamel Proteins - metabolism</topic><topic>Dentistry</topic><topic>Epithelial Cells - cytology</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Medicine</topic><topic>Original Article</topic><topic>Osteogenesis - physiology</topic><topic>Phenotype</topic><topic>Stem Cells - cytology</topic><topic>Tooth, Deciduous - cytology</topic><topic>Transforming Growth Factor beta1 - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Farea, Manal</creatorcontrib><creatorcontrib>Husein, Adam</creatorcontrib><creatorcontrib>Halim, Ahmad Sukari</creatorcontrib><creatorcontrib>Berahim, Zurairah</creatorcontrib><creatorcontrib>Nurul, Asma Abdullah</creatorcontrib><creatorcontrib>Mokhtar, Khairani Idah</creatorcontrib><creatorcontrib>Mokhtar, Kasmawati</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Farea, Manal</au><au>Husein, Adam</au><au>Halim, Ahmad Sukari</au><au>Berahim, Zurairah</au><au>Nurul, Asma Abdullah</au><au>Mokhtar, Khairani Idah</au><au>Mokhtar, Kasmawati</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2016-07-01</date><risdate>2016</risdate><volume>20</volume><issue>6</issue><spage>1181</spage><epage>1191</epage><pages>1181-1191</pages><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β
1
(TGF-β
1
) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED).
Materials and methods
SHED were co-cultured with ERM with/without TGF-β
1
. Then, SHED proliferation, morphological appearance, alkaline phosphatase (
ALP
) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated.
Results
TGF-β
1
enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β
1
. This activity was demonstrated by high
ALP
activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e.
ALP
, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein).
Conclusions
ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β
1
.
Clinical relevance
The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26392396</pmid><doi>10.1007/s00784-015-1601-6</doi><tpages>11</tpages></addata></record> |
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source | MEDLINE; Springer Nature - Complete Springer Journals |
subjects | Cell Differentiation - physiology Cell Proliferation - physiology Coculture Techniques Dental Cementum - cytology Dental Enamel Proteins - metabolism Dentistry Epithelial Cells - cytology Gene Expression Humans Medicine Original Article Osteogenesis - physiology Phenotype Stem Cells - cytology Tooth, Deciduous - cytology Transforming Growth Factor beta1 - pharmacology |
title | Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells |
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