Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells

Objectives The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β 1 (TGF-β 1 ) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED). Mate...

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Veröffentlicht in:Clinical oral investigations 2016-07, Vol.20 (6), p.1181-1191
Hauptverfasser: Farea, Manal, Husein, Adam, Halim, Ahmad Sukari, Berahim, Zurairah, Nurul, Asma Abdullah, Mokhtar, Khairani Idah, Mokhtar, Kasmawati
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container_end_page 1191
container_issue 6
container_start_page 1181
container_title Clinical oral investigations
container_volume 20
creator Farea, Manal
Husein, Adam
Halim, Ahmad Sukari
Berahim, Zurairah
Nurul, Asma Abdullah
Mokhtar, Khairani Idah
Mokhtar, Kasmawati
description Objectives The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β 1 (TGF-β 1 ) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED). Materials and methods SHED were co-cultured with ERM with/without TGF-β 1 . Then, SHED proliferation, morphological appearance, alkaline phosphatase ( ALP ) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated. Results TGF-β 1 enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β 1 . This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP , collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein). Conclusions ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β 1 . Clinical relevance The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.
doi_str_mv 10.1007/s00784-015-1601-6
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Materials and methods SHED were co-cultured with ERM with/without TGF-β 1 . Then, SHED proliferation, morphological appearance, alkaline phosphatase ( ALP ) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated. Results TGF-β 1 enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β 1 . This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP , collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein). Conclusions ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β 1 . Clinical relevance The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-015-1601-6</identifier><identifier>PMID: 26392396</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Cell Differentiation - physiology ; Cell Proliferation - physiology ; Coculture Techniques ; Dental Cementum - cytology ; Dental Enamel Proteins - metabolism ; Dentistry ; Epithelial Cells - cytology ; Gene Expression ; Humans ; Medicine ; Original Article ; Osteogenesis - physiology ; Phenotype ; Stem Cells - cytology ; Tooth, Deciduous - cytology ; Transforming Growth Factor beta1 - pharmacology</subject><ispartof>Clinical oral investigations, 2016-07, Vol.20 (6), p.1181-1191</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-28dc46e452bf98597bac71b0fc43b4e6a65b6b59f2bf803b5ed7fb960ff36ffd3</citedby><cites>FETCH-LOGICAL-c405t-28dc46e452bf98597bac71b0fc43b4e6a65b6b59f2bf803b5ed7fb960ff36ffd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-015-1601-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-015-1601-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26392396$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Farea, Manal</creatorcontrib><creatorcontrib>Husein, Adam</creatorcontrib><creatorcontrib>Halim, Ahmad Sukari</creatorcontrib><creatorcontrib>Berahim, Zurairah</creatorcontrib><creatorcontrib>Nurul, Asma Abdullah</creatorcontrib><creatorcontrib>Mokhtar, Khairani Idah</creatorcontrib><creatorcontrib>Mokhtar, Kasmawati</creatorcontrib><title>Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β 1 (TGF-β 1 ) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED). Materials and methods SHED were co-cultured with ERM with/without TGF-β 1 . Then, SHED proliferation, morphological appearance, alkaline phosphatase ( ALP ) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated. Results TGF-β 1 enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β 1 . This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP , collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein). Conclusions ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β 1 . 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Materials and methods SHED were co-cultured with ERM with/without TGF-β 1 . Then, SHED proliferation, morphological appearance, alkaline phosphatase ( ALP ) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated. Results TGF-β 1 enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β 1 . This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP , collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein). Conclusions ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β 1 . Clinical relevance The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26392396</pmid><doi>10.1007/s00784-015-1601-6</doi><tpages>11</tpages></addata></record>
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subjects Cell Differentiation - physiology
Cell Proliferation - physiology
Coculture Techniques
Dental Cementum - cytology
Dental Enamel Proteins - metabolism
Dentistry
Epithelial Cells - cytology
Gene Expression
Humans
Medicine
Original Article
Osteogenesis - physiology
Phenotype
Stem Cells - cytology
Tooth, Deciduous - cytology
Transforming Growth Factor beta1 - pharmacology
title Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells
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