Development of gene-based markers for the Turnip mosaic virus resistance gene retr02 in Brassica rapa
Turnip mosaic virus (TuMV) is responsible for a serious disease that affects the production of Chinese cabbage. Previous studies have cloned a series of TuMV resistance genes and developed molecular markers. In this study, a derived cleaved amplified polymorphism sequence (dCAPS) marker and a Kompet...
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| Veröffentlicht in: | Plant breeding 2016-08, Vol.135 (4), p.466-470 |
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| container_title | Plant breeding |
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| creator | Li, Guo-Liang Qian, Wei Zhang, Shu-Jiang Zhang, Shi-Fan Li, Fei Zhang, Hui Wu, Jian Wang, Xiao-Wu Sun, Ri-Fei |
| description | Turnip mosaic virus (TuMV) is responsible for a serious disease that affects the production of Chinese cabbage. Previous studies have cloned a series of TuMV resistance genes and developed molecular markers. In this study, a derived cleaved amplified polymorphism sequence (dCAPS) marker and a Kompetitive Allele Specific PCR (KASP) marker were developed based on a single recessive gene, retr02, which confers broad‐spectrum TuMV resistance in Chinese cabbage by means of an additional G at the junction of exon 1 and intron 1. The two markers were able to detect the retr02 allele in Chinese cabbage accessions used in breeding programmes. Compared with the dCAPS marker, the KASP marker was flexible, cost‐effective and quick to process, which is likely to be beneficial in establishing high‐throughput assays for marker‐assisted selection. |
| doi_str_mv | 10.1111/pbr.12372 |
| format | Article |
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Previous studies have cloned a series of TuMV resistance genes and developed molecular markers. In this study, a derived cleaved amplified polymorphism sequence (dCAPS) marker and a Kompetitive Allele Specific PCR (KASP) marker were developed based on a single recessive gene, retr02, which confers broad‐spectrum TuMV resistance in Chinese cabbage by means of an additional G at the junction of exon 1 and intron 1. The two markers were able to detect the retr02 allele in Chinese cabbage accessions used in breeding programmes. Compared with the dCAPS marker, the KASP marker was flexible, cost‐effective and quick to process, which is likely to be beneficial in establishing high‐throughput assays for marker‐assisted selection.</description><identifier>ISSN: 0179-9541</identifier><identifier>EISSN: 1439-0523</identifier><identifier>DOI: 10.1111/pbr.12372</identifier><language>eng</language><publisher>Berlin: Blackwell Publishing Ltd</publisher><subject>Brassica ; Brassica rapa ; dCAPS ; Genes ; Kompetitive Allele Specific PCR ; molecular marker-assisted selection ; retr02 ; Turnip mosaic virus</subject><ispartof>Plant breeding, 2016-08, Vol.135 (4), p.466-470</ispartof><rights>2016 Blackwell Verlag GmbH</rights><rights>Copyright © 2016 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4342-df41de9ca125f0a5383b8466e20eabad31f2665381fd01a27b5b9a4b090f1c213</citedby><cites>FETCH-LOGICAL-c4342-df41de9ca125f0a5383b8466e20eabad31f2665381fd01a27b5b9a4b090f1c213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fpbr.12372$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fpbr.12372$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><contributor>Snowdon, R.</contributor><creatorcontrib>Li, Guo-Liang</creatorcontrib><creatorcontrib>Qian, Wei</creatorcontrib><creatorcontrib>Zhang, Shu-Jiang</creatorcontrib><creatorcontrib>Zhang, Shi-Fan</creatorcontrib><creatorcontrib>Li, Fei</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Wu, Jian</creatorcontrib><creatorcontrib>Wang, Xiao-Wu</creatorcontrib><creatorcontrib>Sun, Ri-Fei</creatorcontrib><title>Development of gene-based markers for the Turnip mosaic virus resistance gene retr02 in Brassica rapa</title><title>Plant breeding</title><addtitle>Plant Breed</addtitle><description>Turnip mosaic virus (TuMV) is responsible for a serious disease that affects the production of Chinese cabbage. Previous studies have cloned a series of TuMV resistance genes and developed molecular markers. In this study, a derived cleaved amplified polymorphism sequence (dCAPS) marker and a Kompetitive Allele Specific PCR (KASP) marker were developed based on a single recessive gene, retr02, which confers broad‐spectrum TuMV resistance in Chinese cabbage by means of an additional G at the junction of exon 1 and intron 1. The two markers were able to detect the retr02 allele in Chinese cabbage accessions used in breeding programmes. Compared with the dCAPS marker, the KASP marker was flexible, cost‐effective and quick to process, which is likely to be beneficial in establishing high‐throughput assays for marker‐assisted selection.</description><subject>Brassica</subject><subject>Brassica rapa</subject><subject>dCAPS</subject><subject>Genes</subject><subject>Kompetitive Allele Specific PCR</subject><subject>molecular marker-assisted selection</subject><subject>retr02</subject><subject>Turnip mosaic virus</subject><issn>0179-9541</issn><issn>1439-0523</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kMtOwzAQRS0EEuWx4A8ssYFFqMdO4mRJeRSkipeKWFpOMgZDmgQ7LfD3uBRYIOGNda1zrZlDyB6wIwhn2BXuCLiQfI0MIBZ5xBIu1smAgcyjPIlhk2x5_8yWWcgBwVNcYN12M2x62hr6iA1GhfZY0Zl2L-g8Na2j_RPS6dw1tqOz1mtb0oV1c08deut73ZT41Qy5d4xT29CR097bUlOnO71DNoyuPe5-39vk_vxsenIRTa7HlyfHk6iMRcyjysRQYV5q4IlhOhGZKLI4TZEz1IWuBBiepuEZTMVAc1kkRa7jguXMQMlBbJOD1b-da1_n6Hs1s77EutYNtnOvIAPI8kRmMqD7f9DnNiwYpltSwVouMx6owxVVutZ7h0Z1zgYxHwqYWgpXQbj6Eh7Y4Yp9szV-_A-qm9HdTyNaNYJDfP9tBO8qlUIm6uFqrO4uRtPb0XmixuITdUuQmg</recordid><startdate>201608</startdate><enddate>201608</enddate><creator>Li, Guo-Liang</creator><creator>Qian, Wei</creator><creator>Zhang, Shu-Jiang</creator><creator>Zhang, Shi-Fan</creator><creator>Li, Fei</creator><creator>Zhang, Hui</creator><creator>Wu, Jian</creator><creator>Wang, Xiao-Wu</creator><creator>Sun, Ri-Fei</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>201608</creationdate><title>Development of gene-based markers for the Turnip mosaic virus resistance gene retr02 in Brassica rapa</title><author>Li, Guo-Liang ; Qian, Wei ; Zhang, Shu-Jiang ; Zhang, Shi-Fan ; Li, Fei ; Zhang, Hui ; Wu, Jian ; Wang, Xiao-Wu ; Sun, Ri-Fei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4342-df41de9ca125f0a5383b8466e20eabad31f2665381fd01a27b5b9a4b090f1c213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Brassica</topic><topic>Brassica rapa</topic><topic>dCAPS</topic><topic>Genes</topic><topic>Kompetitive Allele Specific PCR</topic><topic>molecular marker-assisted selection</topic><topic>retr02</topic><topic>Turnip mosaic virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Guo-Liang</creatorcontrib><creatorcontrib>Qian, Wei</creatorcontrib><creatorcontrib>Zhang, Shu-Jiang</creatorcontrib><creatorcontrib>Zhang, Shi-Fan</creatorcontrib><creatorcontrib>Li, Fei</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Wu, Jian</creatorcontrib><creatorcontrib>Wang, Xiao-Wu</creatorcontrib><creatorcontrib>Sun, Ri-Fei</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Plant breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Guo-Liang</au><au>Qian, Wei</au><au>Zhang, Shu-Jiang</au><au>Zhang, Shi-Fan</au><au>Li, Fei</au><au>Zhang, Hui</au><au>Wu, Jian</au><au>Wang, Xiao-Wu</au><au>Sun, Ri-Fei</au><au>Snowdon, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of gene-based markers for the Turnip mosaic virus resistance gene retr02 in Brassica rapa</atitle><jtitle>Plant breeding</jtitle><addtitle>Plant Breed</addtitle><date>2016-08</date><risdate>2016</risdate><volume>135</volume><issue>4</issue><spage>466</spage><epage>470</epage><pages>466-470</pages><issn>0179-9541</issn><eissn>1439-0523</eissn><abstract>Turnip mosaic virus (TuMV) is responsible for a serious disease that affects the production of Chinese cabbage. Previous studies have cloned a series of TuMV resistance genes and developed molecular markers. In this study, a derived cleaved amplified polymorphism sequence (dCAPS) marker and a Kompetitive Allele Specific PCR (KASP) marker were developed based on a single recessive gene, retr02, which confers broad‐spectrum TuMV resistance in Chinese cabbage by means of an additional G at the junction of exon 1 and intron 1. The two markers were able to detect the retr02 allele in Chinese cabbage accessions used in breeding programmes. Compared with the dCAPS marker, the KASP marker was flexible, cost‐effective and quick to process, which is likely to be beneficial in establishing high‐throughput assays for marker‐assisted selection.</abstract><cop>Berlin</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/pbr.12372</doi><tpages>5</tpages></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Brassica Brassica rapa dCAPS Genes Kompetitive Allele Specific PCR molecular marker-assisted selection retr02 Turnip mosaic virus |
| title | Development of gene-based markers for the Turnip mosaic virus resistance gene retr02 in Brassica rapa |
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