Enhanced activity of Thermomyces lanuginosus lipase by site-saturation mutagenesis for efficient biosynthesis of chiral intermediate of pregabalin

Thermomyces lanuginosus lipase (TLL) variants with enhanced activity for kinetic resolution of 2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester (CNDE) were constructed by site-saturation mutagenesis. Single mutant S83T and double mutant S58L/S83T exhibited 2.69 and 5.46-fold improvement in t...

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Veröffentlicht in:	Biochemical engineering journal 2016-09, Vol.113, p.12-18
Hauptverfasser:	Zheng, Ren-Chao, Ruan, Li-Tao, Ma, Hong-Ye, Tang, Xiao-Ling, Zheng, Yu-Guo
Format:	Artikel
Sprache:	eng
Schlagworte:	Escherichia coli Thermomyces lanuginosus
Online-Zugang:	Volltext
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