Improvement in adiposity with oligofructose is modified by antibiotics in obese rats
ABSTRACT Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health. Using antibiotics and the prebiotic oligofructose, which has been shown to counteract exc...
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| Veröffentlicht in: | The FASEB journal 2016-08, Vol.30 (8), p.2720-2732 |
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| creator | Bomhof, Marc R. Paul, Heather A. Geuking, Markus B. Eller, Lindsay K. Reimer, Raylene A. |
| description | ABSTRACT
Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health. Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota‐dependent effects of oligofructose on body composition and host metabolism. Diet‐induced obese male Sprague Dawley rats, fed a background high‐fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high‐energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose. Combining oligofructose with ampicillin treatment blunted the decrease in adiposity seen with oligofructose. Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose‐induced increases in Bifidobacterium and Lactobacillus. In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose‐induced decrease in adiposity. Oligofructose‐mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.—Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats. FASEB J. 30, 2720‐2732 (2016). www.fasebj.org |
| doi_str_mv | 10.1096/fj.201600151R |
| format | Article |
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Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health. Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota‐dependent effects of oligofructose on body composition and host metabolism. Diet‐induced obese male Sprague Dawley rats, fed a background high‐fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high‐energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose. Combining oligofructose with ampicillin treatment blunted the decrease in adiposity seen with oligofructose. Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose‐induced increases in Bifidobacterium and Lactobacillus. In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose‐induced decrease in adiposity. Oligofructose‐mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.—Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats. FASEB J. 30, 2720‐2732 (2016). www.fasebj.org</description><identifier>ISSN: 0892-6638</identifier><identifier>EISSN: 1530-6860</identifier><identifier>DOI: 10.1096/fj.201600151R</identifier><identifier>PMID: 27059718</identifier><language>eng</language><publisher>United States: Federation of American Societies for Experimental Biology</publisher><subject>Adiposity - drug effects ; Ampicillin - administration & dosage ; Ampicillin - pharmacokinetics ; Animals ; Anti-Bacterial Agents - administration & dosage ; Anti-Bacterial Agents - pharmacokinetics ; Bacteria - drug effects ; Bacteria - genetics ; Bacteria - isolation & purification ; Bifidobacterium ; Blood Glucose ; Energy Intake ; Glucose Tolerance Test ; gut microbiota ; Lactobacillus ; Male ; Neomycin - administration & dosage ; Neomycin - pharmacokinetics ; Obesity - drug therapy ; Oligosaccharides - administration & dosage ; Oligosaccharides - pharmacokinetics ; Oligosaccharides - pharmacology ; prebiotics ; Rats ; Rats, Sprague-Dawley ; RNA, Ribosomal, 16S</subject><ispartof>The FASEB journal, 2016-08, Vol.30 (8), p.2720-2732</ispartof><rights>FASEB</rights><rights>FASEB.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417R-2f83960ad2dce0e048d124b8aa5210f2b207001c136accdfa72e0086a215603a3</citedby><cites>FETCH-LOGICAL-c417R-2f83960ad2dce0e048d124b8aa5210f2b207001c136accdfa72e0086a215603a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1096%2Ffj.201600151R$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1096%2Ffj.201600151R$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27059718$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bomhof, Marc R.</creatorcontrib><creatorcontrib>Paul, Heather A.</creatorcontrib><creatorcontrib>Geuking, Markus B.</creatorcontrib><creatorcontrib>Eller, Lindsay K.</creatorcontrib><creatorcontrib>Reimer, Raylene A.</creatorcontrib><title>Improvement in adiposity with oligofructose is modified by antibiotics in obese rats</title><title>The FASEB journal</title><addtitle>FASEB J</addtitle><description>ABSTRACT
Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health. Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota‐dependent effects of oligofructose on body composition and host metabolism. Diet‐induced obese male Sprague Dawley rats, fed a background high‐fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high‐energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose. Combining oligofructose with ampicillin treatment blunted the decrease in adiposity seen with oligofructose. Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose‐induced increases in Bifidobacterium and Lactobacillus. In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose‐induced decrease in adiposity. Oligofructose‐mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.—Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats. FASEB J. 30, 2720‐2732 (2016). www.fasebj.org</description><subject>Adiposity - drug effects</subject><subject>Ampicillin - administration & dosage</subject><subject>Ampicillin - pharmacokinetics</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - administration & dosage</subject><subject>Anti-Bacterial Agents - pharmacokinetics</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bifidobacterium</subject><subject>Blood Glucose</subject><subject>Energy Intake</subject><subject>Glucose Tolerance Test</subject><subject>gut microbiota</subject><subject>Lactobacillus</subject><subject>Male</subject><subject>Neomycin - administration & dosage</subject><subject>Neomycin - pharmacokinetics</subject><subject>Obesity - drug therapy</subject><subject>Oligosaccharides - administration & dosage</subject><subject>Oligosaccharides - pharmacokinetics</subject><subject>Oligosaccharides - pharmacology</subject><subject>prebiotics</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Ribosomal, 16S</subject><issn>0892-6638</issn><issn>1530-6860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0M9PwjAYxvHGaATRo1ezo5fh-3as6-JJiSiGxATxvHRdqyUbxXWT7L-3BPxxMp56-fSb9iHkHGGIkLIrvRxSQAaAMc4PSB_jCELGGRySPvCUhoxFvEdOnFuCR54ekx5NIE4T5H2ymFbr2n6oSq2awKwCUZi1dabpgo1p3gJbmler61Y21qnAuKCyhdFGFUHeBWLVmNzYxki3vWpz5U0tGndKjrQonTrbnwPyMrlbjB_C2dP9dHwzC-UIk3lINY9SBqKghVSgYMQLpKOcCxFTBE1zCol_ssSICSkLLRKqADgTFGMGkYgG5HLX9V94b5Vrsso4qcpSrJRtXYYckXNOOfsH9WGgyFJPwx2VtXWuVjpb16YSdZchZNvNM73Mfjb3_mKfbvNKFd_6a2QPrndgY0rV_V3LJs-3dPL4K_8JhLeNXA</recordid><startdate>201608</startdate><enddate>201608</enddate><creator>Bomhof, Marc R.</creator><creator>Paul, Heather A.</creator><creator>Geuking, Markus B.</creator><creator>Eller, Lindsay K.</creator><creator>Reimer, Raylene A.</creator><general>Federation of American Societies for Experimental Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201608</creationdate><title>Improvement in adiposity with oligofructose is modified by antibiotics in obese rats</title><author>Bomhof, Marc R. ; Paul, Heather A. ; Geuking, Markus B. ; Eller, Lindsay K. ; Reimer, Raylene A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417R-2f83960ad2dce0e048d124b8aa5210f2b207001c136accdfa72e0086a215603a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adiposity - drug effects</topic><topic>Ampicillin - administration & dosage</topic><topic>Ampicillin - pharmacokinetics</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - administration & dosage</topic><topic>Anti-Bacterial Agents - pharmacokinetics</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Bifidobacterium</topic><topic>Blood Glucose</topic><topic>Energy Intake</topic><topic>Glucose Tolerance Test</topic><topic>gut microbiota</topic><topic>Lactobacillus</topic><topic>Male</topic><topic>Neomycin - administration & dosage</topic><topic>Neomycin - pharmacokinetics</topic><topic>Obesity - drug therapy</topic><topic>Oligosaccharides - administration & dosage</topic><topic>Oligosaccharides - pharmacokinetics</topic><topic>Oligosaccharides - pharmacology</topic><topic>prebiotics</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Ribosomal, 16S</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bomhof, Marc R.</creatorcontrib><creatorcontrib>Paul, Heather A.</creatorcontrib><creatorcontrib>Geuking, Markus B.</creatorcontrib><creatorcontrib>Eller, Lindsay K.</creatorcontrib><creatorcontrib>Reimer, Raylene A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>The FASEB journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bomhof, Marc R.</au><au>Paul, Heather A.</au><au>Geuking, Markus B.</au><au>Eller, Lindsay K.</au><au>Reimer, Raylene A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improvement in adiposity with oligofructose is modified by antibiotics in obese rats</atitle><jtitle>The FASEB journal</jtitle><addtitle>FASEB J</addtitle><date>2016-08</date><risdate>2016</risdate><volume>30</volume><issue>8</issue><spage>2720</spage><epage>2732</epage><pages>2720-2732</pages><issn>0892-6638</issn><eissn>1530-6860</eissn><abstract>ABSTRACT
Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health. Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota‐dependent effects of oligofructose on body composition and host metabolism. Diet‐induced obese male Sprague Dawley rats, fed a background high‐fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high‐energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose. Combining oligofructose with ampicillin treatment blunted the decrease in adiposity seen with oligofructose. Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose‐induced increases in Bifidobacterium and Lactobacillus. In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose‐induced decrease in adiposity. Oligofructose‐mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.—Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats. FASEB J. 30, 2720‐2732 (2016). www.fasebj.org</abstract><cop>United States</cop><pub>Federation of American Societies for Experimental Biology</pub><pmid>27059718</pmid><doi>10.1096/fj.201600151R</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adiposity - drug effects Ampicillin - administration & dosage Ampicillin - pharmacokinetics Animals Anti-Bacterial Agents - administration & dosage Anti-Bacterial Agents - pharmacokinetics Bacteria - drug effects Bacteria - genetics Bacteria - isolation & purification Bifidobacterium Blood Glucose Energy Intake Glucose Tolerance Test gut microbiota Lactobacillus Male Neomycin - administration & dosage Neomycin - pharmacokinetics Obesity - drug therapy Oligosaccharides - administration & dosage Oligosaccharides - pharmacokinetics Oligosaccharides - pharmacology prebiotics Rats Rats, Sprague-Dawley RNA, Ribosomal, 16S |
| title | Improvement in adiposity with oligofructose is modified by antibiotics in obese rats |
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