Fluorescent labelling of in situ hybridisation probes through the copper-catalysed azide-alkyne cycloaddition reaction

In situ hybridisation is a powerful tool to investigate the genome and chromosome architecture. Nick translation (NT) is widely used to label DNA probes for fluorescence in situ hybridisation (FISH). However, NT is limited to the use of long double-stranded DNA and does not allow the labelling of si...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Chromosome research 2016-09, Vol.24 (3), p.299-307
Hauptverfasser:	Hesse, Susann, Manetto, Antonio, Cassinelli, Valentina, Fuchs, Jörg, Ma, Lu, Raddaoui, Nada, Houben, Andreas
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkynes - chemistry Animal Genetics and Genomics Arabidopsis - genetics Azides - chemistry Biomedical and Life Sciences Catalysis Cell Biology Cell Proliferation - genetics Chromosomes Chromosomes, Plant - genetics Copper Copper - chemistry Cycloaddition Reaction - methods Deoxyuracil Nucleotides DNA Probes - chemistry Fluorescence in situ hybridization Hordeum - genetics Human Genetics In Situ Hybridization, Fluorescence - methods Life Sciences Plant Genetics and Genomics Secale - genetics Triticum - genetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!