Treatment for diabetic ulcer wounds using a fern tannin optimized hydrogel formulation with antibacterial and antioxidative properties
Blechnum orientale Linn. (B. orientale) is a fern traditionally used by the natives as a poultice to treat wounds, boils, ulcers, blisters, abscesses, and sores on the skin. To investigate the wound healing ability of a concentrated extract of B. orientale in a hydrogel formulation in healing diabet...
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| Veröffentlicht in: | Journal of ethnopharmacology 2016-08, Vol.189, p.277-289 |
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| creator | Lai, Jordan Chia-Yung Lai, How-Yee Rao, Nalamolu Koteswara Ng, Shiow-Fern |
| description | Blechnum orientale Linn. (B. orientale) is a fern traditionally used by the natives as a poultice to treat wounds, boils, ulcers, blisters, abscesses, and sores on the skin.
To investigate the wound healing ability of a concentrated extract of B. orientale in a hydrogel formulation in healing diabetic ulcer wounds.
The water extract from the leaves of B. orientale was separated from the crude methanolic extract and subjected to flash column chromatography techniques to produce concentrated fractions. These fractions were tested for phytochemical composition, tannin content, antioxidative and antibacterial activity. The bioactive fraction was formulated into a sodium carboxymethylcellulose hydrogel. The extract-loaded hydrogels were then characterized and tested on excision ulcer wounds of streptozotocin-induced diabetic rats. Wound size was measured for 14 days. Histopathological studies were conducted on the healed wound tissues to observe for epithelisation, fibroblast proliferation and angiogenesis. All possible mean values were subjected to statistical analysis using One-way ANOVA and post-hoc with Tukey’s T-test (P |
| doi_str_mv | 10.1016/j.jep.2016.05.032 |
| format | Article |
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To investigate the wound healing ability of a concentrated extract of B. orientale in a hydrogel formulation in healing diabetic ulcer wounds.
The water extract from the leaves of B. orientale was separated from the crude methanolic extract and subjected to flash column chromatography techniques to produce concentrated fractions. These fractions were tested for phytochemical composition, tannin content, antioxidative and antibacterial activity. The bioactive fraction was formulated into a sodium carboxymethylcellulose hydrogel. The extract-loaded hydrogels were then characterized and tested on excision ulcer wounds of streptozotocin-induced diabetic rats. Wound size was measured for 14 days. Histopathological studies were conducted on the healed wound tissues to observe for epithelisation, fibroblast proliferation and angiogenesis. All possible mean values were subjected to statistical analysis using One-way ANOVA and post-hoc with Tukey’s T-test (P<0.05).
One fraction exhibited strong antioxidative and antibacterial activity. The fraction was also highly saturated with tannins, particularly condensed tannins. Fraction W5–1 exhibited stronger antioxidant activity compared to three standards (α-Tocopherol, BHT and Trolox-C). Antibacterial activity was also present, and notably bactericidal towards Methicillin-resistant Staphylococcus aureus (MRSA) at 0.25mg/ml. The extract-loaded hydrogels exhibited shear-thinning properties, with high moisture retention ability. The bioactive fraction at 4% w/w was shown to be able to close diabetic wounds by Day 12 on average. Other groups, including controls, only exhibited wound closure by Day 14 (or not at all). Histopathological studies had also shown that extract-treated wounds exhibited re-epithelisation, higher fibroblast proliferation, collagen synthesis, and angiogenesis.
The ethnopharmacological effects of using B. orientale as a topical treatment for external wounds was validated and was also significantly effective in treating diabetic ulcer wounds. Thus, B. orientale extract hydrogel may be presented as a potential treatment for diabetic ulcer wounds.
[Display omitted]</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2016.05.032</identifier><identifier>PMID: 27208868</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Anti-Bacterial Agents - isolation & purification ; Anti-Bacterial Agents - pharmacology ; Antioxidants - isolation & purification ; Antioxidants - pharmacology ; Bacteria - drug effects ; Bacteria - growth & development ; Biphenyl Compounds - chemistry ; Blechnum orientale linn ; Cell Proliferation - drug effects ; Collagen - metabolism ; Diabetes ; Diabetes Mellitus, Experimental - chemically induced ; Diabetic Angiopathies - drug therapy ; Diabetic Angiopathies - etiology ; Diabetic Angiopathies - metabolism ; Diabetic Angiopathies - pathology ; Diabetic ulcers ; Ferns - chemistry ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Fibroblasts - pathology ; Hydrogel ; Hydrogels ; Hydroxyproline - metabolism ; Male ; Microbial Sensitivity Tests ; Neovascularization, Physiologic - drug effects ; Phytotherapy ; Picrates - chemistry ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Plants, Medicinal ; Rats, Sprague-Dawley ; Re-Epithelialization - drug effects ; Skin - drug effects ; Skin - metabolism ; Skin - pathology ; Streptozocin ; Tannins - isolation & purification ; Tannins - pharmacology ; Time Factors ; Topical formulation ; Ulcer - drug therapy ; Ulcer - etiology ; Ulcer - metabolism ; Ulcer - pathology ; Wound healing ; Wound Healing - drug effects</subject><ispartof>Journal of ethnopharmacology, 2016-08, Vol.189, p.277-289</ispartof><rights>2016 Elsevier Ireland Ltd</rights><rights>Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-413d9a4bdc2f08bd379138adebcf8d33e63b91f585ae91e5baed2e76d82a83753</citedby><cites>FETCH-LOGICAL-c353t-413d9a4bdc2f08bd379138adebcf8d33e63b91f585ae91e5baed2e76d82a83753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S037887411630304X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27208868$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lai, Jordan Chia-Yung</creatorcontrib><creatorcontrib>Lai, How-Yee</creatorcontrib><creatorcontrib>Rao, Nalamolu Koteswara</creatorcontrib><creatorcontrib>Ng, Shiow-Fern</creatorcontrib><title>Treatment for diabetic ulcer wounds using a fern tannin optimized hydrogel formulation with antibacterial and antioxidative properties</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Blechnum orientale Linn. (B. orientale) is a fern traditionally used by the natives as a poultice to treat wounds, boils, ulcers, blisters, abscesses, and sores on the skin.
To investigate the wound healing ability of a concentrated extract of B. orientale in a hydrogel formulation in healing diabetic ulcer wounds.
The water extract from the leaves of B. orientale was separated from the crude methanolic extract and subjected to flash column chromatography techniques to produce concentrated fractions. These fractions were tested for phytochemical composition, tannin content, antioxidative and antibacterial activity. The bioactive fraction was formulated into a sodium carboxymethylcellulose hydrogel. The extract-loaded hydrogels were then characterized and tested on excision ulcer wounds of streptozotocin-induced diabetic rats. Wound size was measured for 14 days. Histopathological studies were conducted on the healed wound tissues to observe for epithelisation, fibroblast proliferation and angiogenesis. All possible mean values were subjected to statistical analysis using One-way ANOVA and post-hoc with Tukey’s T-test (P<0.05).
One fraction exhibited strong antioxidative and antibacterial activity. The fraction was also highly saturated with tannins, particularly condensed tannins. Fraction W5–1 exhibited stronger antioxidant activity compared to three standards (α-Tocopherol, BHT and Trolox-C). Antibacterial activity was also present, and notably bactericidal towards Methicillin-resistant Staphylococcus aureus (MRSA) at 0.25mg/ml. The extract-loaded hydrogels exhibited shear-thinning properties, with high moisture retention ability. The bioactive fraction at 4% w/w was shown to be able to close diabetic wounds by Day 12 on average. Other groups, including controls, only exhibited wound closure by Day 14 (or not at all). Histopathological studies had also shown that extract-treated wounds exhibited re-epithelisation, higher fibroblast proliferation, collagen synthesis, and angiogenesis.
The ethnopharmacological effects of using B. orientale as a topical treatment for external wounds was validated and was also significantly effective in treating diabetic ulcer wounds. Thus, B. orientale extract hydrogel may be presented as a potential treatment for diabetic ulcer wounds.
[Display omitted]</description><subject>Animals</subject><subject>Anti-Bacterial Agents - isolation & purification</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antioxidants - isolation & purification</subject><subject>Antioxidants - pharmacology</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - growth & development</subject><subject>Biphenyl Compounds - chemistry</subject><subject>Blechnum orientale linn</subject><subject>Cell Proliferation - drug effects</subject><subject>Collagen - metabolism</subject><subject>Diabetes</subject><subject>Diabetes Mellitus, Experimental - chemically induced</subject><subject>Diabetic Angiopathies - drug therapy</subject><subject>Diabetic Angiopathies - etiology</subject><subject>Diabetic Angiopathies - metabolism</subject><subject>Diabetic Angiopathies - pathology</subject><subject>Diabetic ulcers</subject><subject>Ferns - chemistry</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - pathology</subject><subject>Hydrogel</subject><subject>Hydrogels</subject><subject>Hydroxyproline - metabolism</subject><subject>Male</subject><subject>Microbial Sensitivity Tests</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Phytotherapy</subject><subject>Picrates - chemistry</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>Plants, Medicinal</subject><subject>Rats, Sprague-Dawley</subject><subject>Re-Epithelialization - drug effects</subject><subject>Skin - drug effects</subject><subject>Skin - metabolism</subject><subject>Skin - pathology</subject><subject>Streptozocin</subject><subject>Tannins - isolation & purification</subject><subject>Tannins - pharmacology</subject><subject>Time Factors</subject><subject>Topical formulation</subject><subject>Ulcer - drug therapy</subject><subject>Ulcer - etiology</subject><subject>Ulcer - metabolism</subject><subject>Ulcer - pathology</subject><subject>Wound healing</subject><subject>Wound Healing - drug effects</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtu1DAUhi0EokPbB2CDvGST4Esy9ogVqrhJlbopa8uxT9ozSuxgO73wADw3Hqaw7MoXff9_dD5C3nLWcsa3H_btHpZW1GvL-pZJ8YJsuFaiUb2SL8mGSaUbrTp-Qt7kvGeMKd6x1-REKMG03uoN-X2dwJYZQqFjTNSjHaCgo-vkINH7uAaf6Zox3FBLR0iBFhsCBhqXgjP-Ak9vH32KNzAdCuZ1sgVjoPdYbqkNBQfrCiS0U335vz_xAX2F7oAuKS6QCkI-I69GO2U4fzpPyY8vn68vvjWXV1-_X3y6bJzsZWk6Lv3OdoN3YmR68FLtuNTWw-BG7aWErRx2fOx1b2HHoR8seAFq67WwWqpenpL3x946-ucKuZgZs4NpsgHimg3XnOtO9OKA8iPqUsw5wWiWhLNNj4Yzc9Bv9qbqNwf9hvWm6q-Zd0_16zCD_5_457sCH48A1CXvEJLJDiE48JjAFeMjPlP_B1oMmhA</recordid><startdate>20160802</startdate><enddate>20160802</enddate><creator>Lai, Jordan Chia-Yung</creator><creator>Lai, How-Yee</creator><creator>Rao, Nalamolu Koteswara</creator><creator>Ng, Shiow-Fern</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160802</creationdate><title>Treatment for diabetic ulcer wounds using a fern tannin optimized hydrogel formulation with antibacterial and antioxidative properties</title><author>Lai, Jordan Chia-Yung ; Lai, How-Yee ; Rao, Nalamolu Koteswara ; Ng, Shiow-Fern</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-413d9a4bdc2f08bd379138adebcf8d33e63b91f585ae91e5baed2e76d82a83753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - isolation & purification</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antioxidants - isolation & purification</topic><topic>Antioxidants - pharmacology</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - growth & development</topic><topic>Biphenyl Compounds - chemistry</topic><topic>Blechnum orientale linn</topic><topic>Cell Proliferation - drug effects</topic><topic>Collagen - metabolism</topic><topic>Diabetes</topic><topic>Diabetes Mellitus, Experimental - chemically induced</topic><topic>Diabetic Angiopathies - drug therapy</topic><topic>Diabetic Angiopathies - etiology</topic><topic>Diabetic Angiopathies - metabolism</topic><topic>Diabetic Angiopathies - pathology</topic><topic>Diabetic ulcers</topic><topic>Ferns - chemistry</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - pathology</topic><topic>Hydrogel</topic><topic>Hydrogels</topic><topic>Hydroxyproline - metabolism</topic><topic>Male</topic><topic>Microbial Sensitivity Tests</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Phytotherapy</topic><topic>Picrates - chemistry</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>Plants, Medicinal</topic><topic>Rats, Sprague-Dawley</topic><topic>Re-Epithelialization - drug effects</topic><topic>Skin - drug effects</topic><topic>Skin - metabolism</topic><topic>Skin - pathology</topic><topic>Streptozocin</topic><topic>Tannins - isolation & purification</topic><topic>Tannins - pharmacology</topic><topic>Time Factors</topic><topic>Topical formulation</topic><topic>Ulcer - drug therapy</topic><topic>Ulcer - etiology</topic><topic>Ulcer - metabolism</topic><topic>Ulcer - pathology</topic><topic>Wound healing</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lai, Jordan Chia-Yung</creatorcontrib><creatorcontrib>Lai, How-Yee</creatorcontrib><creatorcontrib>Rao, Nalamolu Koteswara</creatorcontrib><creatorcontrib>Ng, Shiow-Fern</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lai, Jordan Chia-Yung</au><au>Lai, How-Yee</au><au>Rao, Nalamolu Koteswara</au><au>Ng, Shiow-Fern</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Treatment for diabetic ulcer wounds using a fern tannin optimized hydrogel formulation with antibacterial and antioxidative properties</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2016-08-02</date><risdate>2016</risdate><volume>189</volume><spage>277</spage><epage>289</epage><pages>277-289</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Blechnum orientale Linn. (B. orientale) is a fern traditionally used by the natives as a poultice to treat wounds, boils, ulcers, blisters, abscesses, and sores on the skin.
To investigate the wound healing ability of a concentrated extract of B. orientale in a hydrogel formulation in healing diabetic ulcer wounds.
The water extract from the leaves of B. orientale was separated from the crude methanolic extract and subjected to flash column chromatography techniques to produce concentrated fractions. These fractions were tested for phytochemical composition, tannin content, antioxidative and antibacterial activity. The bioactive fraction was formulated into a sodium carboxymethylcellulose hydrogel. The extract-loaded hydrogels were then characterized and tested on excision ulcer wounds of streptozotocin-induced diabetic rats. Wound size was measured for 14 days. Histopathological studies were conducted on the healed wound tissues to observe for epithelisation, fibroblast proliferation and angiogenesis. All possible mean values were subjected to statistical analysis using One-way ANOVA and post-hoc with Tukey’s T-test (P<0.05).
One fraction exhibited strong antioxidative and antibacterial activity. The fraction was also highly saturated with tannins, particularly condensed tannins. Fraction W5–1 exhibited stronger antioxidant activity compared to three standards (α-Tocopherol, BHT and Trolox-C). Antibacterial activity was also present, and notably bactericidal towards Methicillin-resistant Staphylococcus aureus (MRSA) at 0.25mg/ml. The extract-loaded hydrogels exhibited shear-thinning properties, with high moisture retention ability. The bioactive fraction at 4% w/w was shown to be able to close diabetic wounds by Day 12 on average. Other groups, including controls, only exhibited wound closure by Day 14 (or not at all). Histopathological studies had also shown that extract-treated wounds exhibited re-epithelisation, higher fibroblast proliferation, collagen synthesis, and angiogenesis.
The ethnopharmacological effects of using B. orientale as a topical treatment for external wounds was validated and was also significantly effective in treating diabetic ulcer wounds. Thus, B. orientale extract hydrogel may be presented as a potential treatment for diabetic ulcer wounds.
[Display omitted]</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>27208868</pmid><doi>10.1016/j.jep.2016.05.032</doi><tpages>13</tpages></addata></record> |
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| ispartof | Journal of ethnopharmacology, 2016-08, Vol.189, p.277-289 |
| issn | 0378-8741 1872-7573 |
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| subjects | Animals Anti-Bacterial Agents - isolation & purification Anti-Bacterial Agents - pharmacology Antioxidants - isolation & purification Antioxidants - pharmacology Bacteria - drug effects Bacteria - growth & development Biphenyl Compounds - chemistry Blechnum orientale linn Cell Proliferation - drug effects Collagen - metabolism Diabetes Diabetes Mellitus, Experimental - chemically induced Diabetic Angiopathies - drug therapy Diabetic Angiopathies - etiology Diabetic Angiopathies - metabolism Diabetic Angiopathies - pathology Diabetic ulcers Ferns - chemistry Fibroblasts - drug effects Fibroblasts - metabolism Fibroblasts - pathology Hydrogel Hydrogels Hydroxyproline - metabolism Male Microbial Sensitivity Tests Neovascularization, Physiologic - drug effects Phytotherapy Picrates - chemistry Plant Extracts - isolation & purification Plant Extracts - pharmacology Plant Leaves - chemistry Plants, Medicinal Rats, Sprague-Dawley Re-Epithelialization - drug effects Skin - drug effects Skin - metabolism Skin - pathology Streptozocin Tannins - isolation & purification Tannins - pharmacology Time Factors Topical formulation Ulcer - drug therapy Ulcer - etiology Ulcer - metabolism Ulcer - pathology Wound healing Wound Healing - drug effects |
| title | Treatment for diabetic ulcer wounds using a fern tannin optimized hydrogel formulation with antibacterial and antioxidative properties |
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